| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , we now found that the human homologue APH 1a carrying the equivalent mutation G122D is fully active in yeast co expressing PS 1 , NCT , and PEN 2 . ^^^ Separate overexpression of PS 1 , NCT , or PEN 2 together with APH 1a G122D allowed the formation of heterodimers lacking the other endogenous components . ^^^ Only the combined overexpression of PS 1 and NCT together with APH 1a G122D facilitated the formation of a fully active gamma secretase complex . ^^^ We conclude that cooperative effects may stabilize a trim eric complex of APH 1a G122D together with PS 1 and NCT . ^^^ |
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| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| When we co infected Sf 9 cells with human presenilin 1 ( PS 1 ) , nicastrin , APH 1a , and PEN 2 , a high molecular weight membrane protein complex that contained PS 1 exclusively in its fragment form associated with three other cofactor proteins was reconstituted and recovered in a highly gamma secretase active state in budded virus particles , whereas nonfunctional PS 1 holoproteins massively contaminated the parental Sf 9 cell membranes . ^^^ |
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| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| We report here that mouse brain contains a complex composed of gamma secretase subunits presenilin 1 N terminal fragment , presenilin 1 C terminal fragment , Nicastrin , Aph 1a and Pen 2 . ^^^ |
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| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| We found that knock down of APH 1a , but not APH 1b , resulted in impaired maturation of nicastrin and reduced expression of presenilin 1 , presenilin 2 , and PEN 2 proteins . ^^^ |
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| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| Characterization of the reconstituted gamma secretase complex from Sf 9 cells co expressing presenilin 1 , nicastrin [ correction of nacastrin ] , aph 1a , and pen 2 . ^^^ The native gamma secretase is a heterogeneous population of large membrane protein complexes containing presenilin 1 ( PS 1 ) or presenilin 2 ( PS 2 ) , aph 1a or aph 1b , nicastrin , and pen 2 . ^^^ Here we report the reconstitution of a gamma secretase complex in Sf 9 cells by co infection with baculoviruses carrying the PS 1 , nicastrin , pen 2 , and aph 1a genes . ^^^ |
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| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| Effects of RNAi mediated silencing of PEN 2 , APH 1a , and nicastrin on wild type vs FAD mutant forms of presenilin 1 . ^^^ In contrast , RNAi for nicastrin and APH 1a decreased PS 1 FL in H 4 cells expressing either wt PS 1 or Delta 9 PS1 . ^^^ |
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| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| Active gamma secretase is a tetrameric protein complex consisting of presenilin 1 ( or 2 ) , nicastrin , PEN 2 , and Aph 1a ( or 1b ) . ^^^ |
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| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| Recent genetic studies have identified a number of additional genes encoding APH1a , APH1b , PEN 2 , and Nicastrin proteins , which are part of the gamma secretase complex with PS 1 . ^^^ |
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| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q96BI3 and P49768 |
Pubmed |
SVM Score :0.0 |
| NA |
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