| Interacting proteins: Q96AE4 and Q9UHX1 |
Pubmed |
SVM Score :0.6379485 |
| Recruited through FBP ' s nucleic acid binding domain , FIR formed a ternary complex with FBP and FUSE . 0.6379485^^^ |
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| Interacting proteins: Q96AE4 and Q9UHX1 |
Pubmed |
SVM Score :0.0 |
| Here , XPB and XPD mutations are shown to block transcription activation by the FUSE Binding Protein ( FBP ) , a regulator of c myc expression , and repression by the FBP Interacting Repressor ( FIR ) . ^^^ Through TFIIH , FBP facilitates transcription until promoter escape , whereas after initiation , FIR uses TFIIH to delay promoter escape . ^^^ Mutations in TFIIH that impair regulation by FBP and FIR affect proper regulation of c myc expression and have implications in the development of malignancy . . ^^^ |
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| Interacting proteins: Q96AE4 and Q9UHX1 |
Pubmed |
SVM Score :0.0 |
| METHODS : FUSE binding protein ( FBP ) interacting repressor ( FIR ) and FBP are c myc transcription factors and are known to interact physically . ^^^ To examine their interaction within viable cells , FIR and the binding motif of FBP , the FBP central domain ( FBPcd ) , were fused with CFP and YFP , respectively , and this pair of fluorescently tagged proteins was used to detect FRET in vivo . ^^^ |
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| Interacting proteins: Q96AE4 and Q9UHX1 |
Pubmed |
SVM Score :0.0 |
| Those features include the use of an expanded proximal promoter , the averaging of input from dozens of transcription factors , and real time feedback using the supercoil deformable Far UpStream Element ( FUSE ) as physical sensor of ongoing transcriptional activity , and the FUSE binding protein ( FBP ) as well as the FBP interacting repressor ( FIR ) as effectors to enforce normal transcription from the c myc promoter . . ^^^ |
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| Interacting proteins: Q96AE4 and Q9UHX1 |
Pubmed |
SVM Score :0.0 |
| The FUSE / FBP / FIR / TFIIH system is a molecular machine programming a pulse of c myc expression . ^^^ Because transcriptionally generated torsion melts FUSE in vitro even in linear DNA , and FBP / FBP Interacting Repressor ( FIR ) regulates transcription through TFIIH , these components have been speculated to be the mechanosensor ( FUSE ) and effectors ( FBP / FIR ) of a real time mechanism controlling c myc transcription . ^^^ To ascertain whether the FUSE / FBP / FIR system operates according to this hypothesis in vivo , the flux of activators , repressors and chromatin remodeling complexes on the c myc promoter was monitored throughout the serum induced pulse of transcription . ^^^ After transcription was switched on by conventional factors and chromatin regulators , FBP and FIR were recruited and established a dynamically remodeled loop with TFIIH at the P 2 promoter . ^^^ The in vitro recruitment of FBP and FIR to dynamically stressed c myc DNA paralleled the in vivo process . . ^^^ |
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| Interacting proteins: Q96AE4 and Q9UHX1 |
Pubmed |
SVM Score :0.0 |
| Although some differences , such as weakened FBP 3 nuclear localization , were predictable from primary sequence differences , the unexpected failure of FBP 3 to bind the FBP interacting repressor ( FIR ) was traced to seemingly conservative substitutions within a small patch of an N terminal alpha helix . ^^^ |
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