| Interacting proteins: Q969D9 and P42229 |
Pubmed |
SVM Score :0.0 |
| In fact , TSLP failed to activate any of the four known Janus family kinases , suggesting that Stat 5 phosphorylation is mediated by a novel mechanism . ^^^ |
|
| Interacting proteins: Q969D9 and P42229 |
Pubmed |
SVM Score :0.0 |
| Although TSLP and IL 7 both induce tyrosine phosphorylation of the transcription factor Stat 5 , only IL 7 mediated signal transduction could be associated with activation of Janus family kinases ( Jaks ) . ^^^ Because Stat 5 phosphorylation following cytokine stimulation is generally mediated by Jaks , the lack of Jak activation after TSLP treatment suggested the possibility that tyrosine phosphorylated Stat 5 may be nonfunctional . ^^^ Herein , we demonstrate that TSLP induces a functional Stat 5 transcription factor in that TSLP stimulation results in Stat 5 DNA complex formation and transcription of the Stat 5 responsive gene CIS . ^^^ We also show that the TSLP receptor complex is functionally reconstituted using TSLP R and IL 7Ralpha and that TSLP mediated signal transduction requires Stat 5 . ^^^ |
|
| Interacting proteins: Q969D9 and P42229 |
Pubmed |
SVM Score :0.0 |
| Cells transfected with both receptor subunits proliferated in response to purified , recombinant human TSLP , with induced phosphorylation of Stat 3 and Stat 5 . ^^^ |
|
| Interacting proteins: Q969D9 and P42229 |
Pubmed |
SVM Score :0.0 |
| In contrast to IL 7 , TSLP triggered STAT 5 phosphorylation was not preceded by activation of janus kinase ( JAK ) 3 . ^^^ These findings would be in accordance with the notion , raised previously for the mouse system , that TSLP leads to STAT 5 phosphorylation by activating other kinases than the JAKs . ^^^ TSLP induced proliferation is preceded by phosphorylation of STAT 5 , but not of JAK 3 . . ^^^ |
|
| Interacting proteins: Q969D9 and P42229 |
Pubmed |
SVM Score :0.0 |
| TSLP mediated signaling is unique among members of the cytokine receptor family in that activation of the transcription factor Stat 5 occurs without detectable Janus kinase activation . ^^^ Using a variety of biological systems we demonstrate here that TSLP mediated Stat 5 activation can be uncoupled from proliferation . ^^^ We also show that the single tyrosine residue in the cytoplasmic domain of the TSLP receptor is critical for TSLP mediated proliferation , but is dispensable for Stat 5 activation . ^^^ Our data demonstrate that TSLP mediated Stat 5 activation is insufficient for cell proliferation and identifies residues within the TSLP receptor complex required to mediate these downstream events . . ^^^ |
|
| Interacting proteins: Q969D9 and P42229 |
Pubmed |
SVM Score :0.0 |
| NA |
|