| A close correlation in the expression patterns of Af 6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovary . ^^^ Af 6 , a cell junction protein , has been identified as a substrate of Usp9x , suggesting a possible association between Usp9x and Af 6 in spermatogenesis and oogenesis . ^^^ In this study , we examined the expression pattern of Af 6 and Usp9x and their intracellular localization in testes and ovaries of mice treated with or without pregnant mare serum gonadotropin ( PMSG ) , an FSH like hormone . ^^^ In Sertoli cells , Af 6 was continuously expressed throughout postnatal and adult stages , where both Af 6 and Usp9x were enriched at the sites of Sertoli Sertoli and Sertoli spermatid junctions especially at stages 11 6 . ^^^ In the granulosa cells , Af 6 , as well as Usp9x , was highly expressed in primordial and primary follicles , but its expression rapidly decreased after the late secondary follicle stage . ^^^ |