| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.84291641 |
| We propose that BRF 1 plays a role in Pol 3 initiation analogous to the role played by TFIIB for Pol 2 in its interaction with TBP and polymerase . 0.84291641^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.95569322 |
| Using recombinant TFIIIB subunits , we found that TFIIIB 90 interacts weakly with TBP in the absence of BRF 1 , and that this interaction is enhanced at least 25 fold by BRF 1 . 0.95569322^^^ In addition , TFIIIB 90 showed pol 3 specificity as it could not interact with the pol 2 specific TFIIB TBP DNA complex . 0.90205962^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.82162672 |
| For yeast Pol 3 transcription , TBP associates with Brf ( from TFIIB related factor ) and B ' ' , two Pol 3 TAFs , to form Pol 3 transcription factor TFIIIB . 0.82162672^^^ Polymerase ( Pol ) 3 TATA box binding protein ( TBP ) associated factor Brf binds to a surface on TBP also required for activated Pol 2 transcription . 0.53010197^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The TATA binding protein , TBP , and the TFIIB related BRF 1 gene product BRF , together reconstitute the transcription factor activity and TFIIC dependent DNA binding activity of the B ' component of TFIIIB . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Yeast contain a single TFIIIB activity that is comprised of the TATA binding protein ( TBP ) , TFIIB related factor 1 ( BRF 1 ) , and TFIIIB ' ' , whereas two distinct TFIIIB activities , TFIIIB alpha and TFIIIB beta , have been described in human cells . ^^^ Human TFIIIB beta is required for transcription of genes with internal promoter elements , and contains TBP , a TFIIIB ' ' homologue ( TFIIIB 150 ) , and a BRF 1 homologue ( TFIIIB 90 ) , whereas TFIIIB alpha is required for transcription of genes with promoter elements upstream of the initiation site . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Although this surface is important for interactions with TFIIA and Brf 1 , TBP interactions with these two factors do not appear to be responsible for this dependence on the N terminal region . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The essential Saccharomyces cerevisiae gene BDP 1 encodes a subunit of RNA polymerase 3 ( Pol 3 ) transcription factor ( TFIIIB ) ; TATA box binding protein ( TBP ) and Brf 1 are the other subunits of this three protein complex . ^^^ Suppression of bdp 1 conditional viability by overexpressing SPT 15 and BRF 1 identified functional interactions of specific Bdp 1 segments with TBP and Brf 1 , respectively . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Transcription factor IIIB ( TFIIIB ) , consisting of the TATA binding protein ( TBP ) , TFIIB related factor ( Brf 1 ) and Bdp 1 , is a central component in basal and regulated transcription by RNA polymerase 3 . ^^^ The amino terminal half of Brf 1 shares a high degree of sequence similarity with the polymerase 2 general transcription factor TFIIB , but it is the carboxy terminal half of Brf 1 that contributes most of its binding affinity with TBP . ^^^ We report here a 2 . 95 A resolution crystal structure of the ternary complex containing Brf 1 homology domain 2 , the conserved region of TBP and 19 base pairs of U 6 promoter DNA . ^^^ The structure reveals the core interface for assembly of TFIIIB and demonstrates how the loosely packed Brf 1 domain achieves remarkable binding specificity with the convex and lateral surfaces of TBP . . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| This is accomplished by the ability of the TFIIIB subunit , TFIIB related factor ( Brf 1 ) , to make stable protein protein interactions with TATA binding protein ( TBP ) and place it on the promoter less upstream DNA . ^^^ In this study we demonstrate that S . pombe ( Sp ) Brf does not form stable interactions with TBP in the absence of DNA using approaches that do reveal stable association of TBP and S . cerevisiae ( Sc ) Brf 1 . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Unlike RNA pol 2 dependent promoter repression , overexpressing TBP can reverse inhibition of tRNA gene transcription by p 53 . p 53 does not disrupt the direct interaction between the TFIIIB subunits TBP and Brf 1 , but prevents the association of Brf 1 complexes with TFIIIC 2 and RNA pol 3 . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| TBP , the transcription factor IIB ( TFIIB ) related factor Brf 1 and the pol 3 specific factor Bdp 1 constitute TFIIIB . ^^^ A photochemical cross linking approach was used to survey a collection of human TBP surface residue mutants for their ability to form TFIIIB DNA complexes reliant on only the TFIIB related part of Brf 1 . ^^^ Structural modeling of the Brf 1 TBP complex and comparison with its TFIIB TBP analog further rationalizes the close resemblance of the TBP interaction with the N proximal part of Brf 1 and TFIIB , and establishes the conserved usage of a TBP surface in pol 2 and pol 3 transcription for a conserved function in the initiation of transcription . . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| In contrast , the third TFIIB family member , Brf 1 , which is required for transcription from TATA less Pol 3 promoters , does not stimulate TBP binding to the TATA box . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| In the case of RNA polymerase 3 transcription , this is due to phosphorylation mediated inactivation of TFIIIB , an essential complex comprising the TATA binding protein TBP and the TAF subunits Brf 1 and Bdp 1 . ^^^ Brf 1 is hyperphosphorylated in metaphase arrested cells , but remains associated with promoters in condensed chromosomes , along with TBP . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The Brf 1 subunit of TFIIIB plays an important role in recruiting the TATA binding protein ( TBP ) to the up stream region of genes transcribed by RNA polymerase 3 . ^^^ Here we show that Saccharomyces cerevisiae Brf 1 induces TBP dimers to dissociate . ^^^ The high affinity TBP binding domain of Brf 1 is not sufficient to promote TBP dimer dissociation but in addition requires the TFIIB homology domain of Brf 1 . ^^^ A model is proposed to explain how two distinct functional domains of Brf 1 work in concert to dissociate TBP into monomers . . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The TBP mutations that are lethal in the absence of Nhp 6 cluster in three regions : on the upper surface of TBP that may have a regulatory role , near residues that contact Spt 3 , or near residues known to contact either TFIIA or Brf 1 ( in TFIIIB ) . ^^^ The latter set of mutations suggests that Nhp 6 becomes essential when a TBP mutant compromises its ability to interact with either TFIIA or Brf 1 . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| A TFIIIC less transcription system , in which TFIIIB was reconstituted from recombinant TATA box binding protein and Brf 1 proteins and a crude fraction containing the Bdp 1 component , was sufficient to direct efficient Pol 3 recycling on short ( approximately 100 bp ) class 3 genes . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Transcription factor ( TF ) IIIB , the central transcription initiation factor of RNA polymerase 3 ( pol 3 ) , is composed of three subunits , Bdp 1 , Brf 1 and TATA binding protein ( TBP ) , all essential for normal function in vivo and in vitro . ^^^ Brf 1 is a modular protein : Its N proximal half is related to TFIIB and binds similarly to the C terminal stirrup of TBP ; its C proximal one third provides most of the affinity for TBP by binding along the entire length of the convex surface and N terminal lateral face of TBP . ^^^ A structure informed triple fusion protein , with TBP core placed between the N and C proximal domains of Brf 1 , has been constructed . ^^^ The Brf 1 TBP triple fusion protein effectively replaces both Brf 1 and TBP in TFIIIC dependent and independent transcription in vitro , and forms extremely stable TFIIIB DNA complexes that are indistinguishable from wild type TFIIIB DNA complexes by chemical nuclease footprinting . ^^^ Unlike Brf 1 and TBP , the triple fusion protein is able to recruit pol 3 for TATA box directed transcription of linear and supercoiled DNA in the absence of Bdp 1 . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| TFIIB bridges RNA polymerase 2 ( Pol 2 ) with the promoter bound preinitiation complex , whereas Brf 1 and Brf 2 are involved , as part of activities also containing TBP and Bdp 1 and referred to here as Brf 1 TFIIIB and Brf 2 TFIIIB , in the recruitment of Pol 3 . ^^^ In yeast Brf 1 , the C terminal extension interacts with the TBP / TATA box complex and contributes to the recruitment of Bdp 1 . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Transcription of the box B less tRNA ( Asn ) genes was strongly influenced by their 5 ' flanking regions , and it was stimulated by TBP and Brf 1 proteins synergistically . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| We provide evidence that large T antigen can interact with the TBP containing pol 3 transcription factor human TFIIB related factor ( hBRF ) , as well as with at least two of the three TAFs in the pol 3 specific small nuclear RNA activating protein complex ( SNAPc ) . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| We report the purification of a human TFIIIB derived complex containing only the TATA binding polypeptide ( TBP ) and a 90 kDa subunit ( TFIIIB 90 ) and the isolation of a cDNA clone encoding the 90 kDa subunit . ^^^ The N terminal half of TFIIIB 90 exhibits sequence similarity to the yeast TFIIIB 70 ( BRF ) and the class 2 transcription factor TFIIB and interacts weakly with TBP . ^^^ The C terminal half of TFIIIB 90 contains a high mobility group protein 2 ( HMG 2 ) related domain and interacts strongly with TBP . ^^^ Recombinant TFIIIB 90 plus recombinant human TBP substitute for human TFIIIB in a complementation assay for transcription of 5S , tRNA , and VA 1 RNA genes , and both the TFIIB related domain and the HMG 2 related domain are required for this activity . ^^^ TFIIIB 90 is also required for transcription of human 7SK and U 6 RNA genes by RNA polymerase 3 , but apparently within a complex distinct from the TBP / TFIIIB90 complex . . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| In addition , immunoprecipitation analyses demonstrate that substantial and similar molar amounts of TATA binding protein ( TBP ) and TFIIIB 90 can weakly associate with PTF at low salt conditions , but this association is dramatically reduced at high salt concentrations . ^^^ Along with our previous demonstration of both physical interactions between PTF gamma / PTF delta and TBP and the involvement of TFIIIB 90 in the transcription of class 3 snRNA genes , these results are consistent with the notion that a TBP containing complex related to TFIIIB is required for the transcription of class 3 snRNA genes , and acts through weak interaction with the four subunit PTF . . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Transcription by RNA polymerase 3 ( pol 3 ) in yeast requires the assembly of an initiation complex comprising the TATA binding protein ( TBP ) , a 90 kDa polypeptide ( TFIIIB 90 ) , and a 70 kDa polypeptide ( TFIIIB 70 ) . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| TFIIIB , the initiation factor for transcription by RNA polymerase 3 ( pol 3 ) is , in yeast , composed of three subunits : TBP , TFIIIB70 / Brf1 and TFIIIB 90 . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The yeast TFIIIB transcription factor is composed of three components , TBP , TFIIIB 90 or B ' ' , and TFIIIB 70 or BRF . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| TFIIIB DNA complexes in yeast comprise the TATA binding protein ( TBP ) , the TFIIB related factor TFIIIB 70 , and TFIIIB 90 . ^^^ The high stability of the TFIIIB DNA complex is conferred by TFIIIB 90 binding to TFIIIB 70 TBP DNA complexes . ^^^ This stability is thought to result from compound bends introduced in the DNA by TBP and TFIIIB 90 and by protein protein interactions that obstruct DNA dissociation . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| In yeast , this factor is composed of three essential polypeptides TBP , TFIIIB 70 and TFIIIB 90 , that are sufficient as recombinant proteins , together with TFIIIC , to promote accurate transcription in vitro . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The central RNA polymerase 3 ( Pol 3 ) transcription factor TFIIIB is composed of the TATA binding protein ( TBP ) , Brf , a protein related to TFIIB , and the product of the newly cloned TFC 5 gene . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The DNase 1 footprints of entirely recombinant TFIIIB , composed of B ' ' , the 67 kDa Brf , and TATA box binding protein , and TFIIIB reconstituted with natural B ' ' are indistinguishable . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| In Saccharomyces cerevisiae , two components of the RNA polymerase 3 ( Pol 3 ) general transcription factor TFIIIB are the TATA binding protein ( TBP ) and the B related factor ( BRF ) , so called because its amino terminal half is homologous to the Pol 2 transcription factor IIB ( TFIIB ) . ^^^ By assaying for interactions between BRF and other Pol 3 transcription factors , we show that it is able to bind to the 135 kD subunit of TFIIIC and also to TBP . ^^^ Surprisingly , in addition to binding the TFIIB homologous amino terminal portion of BRF , TBP also interacts strongly with the carboxy terminal half . ^^^ Furthermore , TBP mutations that selectively inhibit Pol 3 transcription in vivo impair interactions between TBP and the BRF carboxy terminal domain . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The same three components of TFIIIB that are necessary for TFIIIC dependent transcription of tRNA genes ( recombinant TBP and Brf and the denaturing gel purified 90 kDa subunit ) are required and sufficient for TATA box directed U 6 transcription . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| TFIIIB is a multisubunit complex that includes the TATA binding protein ( TBP ) and a TFIIB related factor called BRF . ^^^ We show that RB itself contains regions of homology to both TBP and BRF and propose a model in which RB disrupts TFIIIB by mimicking these two components . . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Even deletion of the entire TFIIB homologous half of the protein , as in Brf ( 317 596 ) and Brf ( 352 596 ) , allows some interaction with DNA bound TBP and with the B ' ' component of TFIIIB to be retained . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| TFIIIB is composed of three subunits , the TATA binding protein ( TBP ) , TFIIB related factor ( BRF ) , and B ' ' . ^^^ Chemical footprinting , as well as mutagenesis of TBP , BRF , and promoter DNA , was used to probe the architecture of TFIIIB subunits bound to DNA . ^^^ BRF bound to TBP DNA through the nonconserved C terminal region and required 15 bp downstream of the TATA box and as little as 1 bp upstream of the TATA box for stable complex formation . ^^^ Hydroxyl radical footprinting of TFIIIB complexes and modeling the results to the TBP DNA structure suggest that BRF and B ' ' surround TBP on both faces of the TBP DNA complex and provide an explanation for the exceptional stability of this complex . ^^^ Competition for binding to TBP by BRF and either TFIIB or TFIIA suggests that BRF binds on the opposite face of the TBP DNA complex from TFIIB and that the binding sites for TFIIA and BRF overlap . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Two essential components of TFIIIB are the TATA binding protein ( TBP ) and an associated polypeptide called BRF that is specific to this complex . ^^^ The abundance of both TBP and BRF decreases during F 9 cell differentiation . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Transcription factor ( TF ) IIIB , which directs RNA polymerase ( pol ) 3 to its promoters , is made up of three components : the TATA box binding protein , the TFIIB related Brf , and the pol 3 specific B ' ' . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| We also identified sites of interaction of Brf fragments with TATA binding protein ( TBP ) , taking advantage of a recently completed mutational analysis of the TBP surface . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The B ' ' and TFIIB related factor ( BRF ) subunits of TFIIIB are positioned on opposite sides of the TBP DNA core of the TFIIIB complex , as indicated by correlation of cross linking data to the crystal structure of the TBP TATA box complex . ^^^ Evidence is given for BRF binding near the C terminal stirrup of TBP , similar to that of TFIIB near the TBP TATA box complex . ^^^ The protein clamp formed around the TBP DNA complex by BRF and B ' ' would help explain the long half life of the TFIIIB DNA complex and its resistance to polyanions and high salt . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| All three subunits of TFIIIB , TBP , Brf ( the TFIIB related subunit ) and B ' ' , are required for transcription of supercoiled and linear duplex DNA , but we show here that B ' ' is non essential on a promoter that has been partly pre opened by unpairing a short segment of the transcription bubble . ^^^ |
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| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| These data suggest that in Drosophila , TRF 1 rather than TBP forms a complex with BRF that plays a major role in RNA pol 3 transcription . . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that the recombinant Brf and TBP subunits of TFIIIB , which interact over the SNR 6 TATA box , direct integration at the SNR 6 transcription initiation site in the absence of detectable TFIIIC or TFIIIB subunit B ' ' . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| It consists of three subunits : the TATA box binding protein ( TBP ) , a TFIIB related factor , BRF , and B ' ' . ^^^ We have isolated cDNAs encoding alternatively spliced forms of human BRF that can complex with TBP . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| RNA polymerase from the hyperthermophile archaeon Pyrococcus furiosus ( Pfu ) forms specific and transcriptionally active complexes with its conjugate transcription factors TBP ( the archaeal TATA binding protein homolog ) and TFB ( the archaeal homolog of eukaryotic RNA polymerase 2 and 3 transcription factors TFIIB and Brf ) at the Pfu glutamate dehydrogenase promoter . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| Although human homologs for two of the TFIIIB subunits , the TATA box binding protein TBP and the TFIIB related factor BRF , have been characterized , a human homolog of yeast B ( `` ) has not been described . ^^^ Saccharomyces cerevisiae TFIIIB is a complex of three subunits , TBP , the TFIIB related factor BRF , and the more loosely associated polypeptide beta ( `` ) . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| The Pol 3 initiation factor Brf is found in complexes with TFIIIC and Pol 3 in S . pombe , while TBP is not , consistent with independent recruitment of TBP by TATA . ^^^ |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92994 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|