| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| In vitro translated purified Vav activated by phorbol myristate acetate ( PMA ) or phosphorylation with recombinant p56lck displayed GEF activity against Ras but not against recombinant RacI , RacII , Ral , or RhoA proteins . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Ectopic expression of a hematopoietic specific guanine nucleotide exchange factor ( GEF ) , Vav , reconstituted the Tacgamma induced , Syk and Rac 1 dependent JNK activation ; and tyrosine phosphorylation of Vav by Syk stimulated its GEF activity for Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Human Duo contains a guanine nucleotide exchange factor ( GEF ) domain that is likely to be rac 1 specific , a pleckstrin homology ( PH ) domain and spectrin like repeat units . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| The invasion inducing T lymphoma invasion and metastasis 1 ( Tiam 1 ) protein functions as a guanine nucleotide exchange factor ( GEF ) for the small GTPase Rac 1 . ^^^ Differentiation dependent expression of Tiam 1 in the developing brain suggests a role for this GEF and its effector Rac 1 in the control of neuronal morphology . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| In vitro , GEF domain 1 ( GEFD 1 ) is specifically active on Rac 1 , while GEF domain 2 ( GEFD 2 ) targets RhoA . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| N terminal truncation of mNET 1 generates an activated transforming form of the protein , mNET1DeltaN , which acts as a GEF for RhoA but not Cdc 42 or Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| We have shown in vitro and in vivo that the first GEF domain ( GEFD 1 ) activates Rac 1 , while the second GEF domain ( GEFD 2 ) acts on RhoA . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Here we show that Ras GRF 1 exhibits Rac 1 specific GEF activity when recovered from cells overexpressing G protein beta gamma subunits ( Gbeta gamma ) . ^^^ Evidence for the involvement of tyrosine kinase ( s ) in Gbeta gamma mediated induction of Rac 1 specific GEF activity was provided by the use of specific inhibitors . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| We report here that KIAA 0380 , a protein with a tandem DH / PH domain , an amino terminal PDZ domain and a regulator of G protein signalling ( RGS ) homology domain , is a specific GEF for RhoA , but not for Rac 1 and Cdc 42 , as determined by GDP release , guanosine 5 ' O ( 3 thio ) triphosphate ( GTPgammaS ) binding and protein binding assays . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Using a classical in vitro exchange assay , TrioD 1 displays a higher GEF activity on RhoG than on Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| In addition , we identified some of the upstream events accompanying the beta 1 integrin mediated p 38 MAPK activation , namely , the activation of the Rac guanine nucleotide exchange factor ( GEF ) p 95 Vav , the small G protein Rac 1 , and the cytoplasmic kinases Pak 1 and MKK 3 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Whereas Vav displayed GEF activity for Rac 1 , Cdc 42 , RhoA , and RhoG , Vav 2 was reported to exhibit GEF activity for RhoA , RhoB , and RhoG but not for Rac 1 or Cdc 42 . ^^^ In contrast to the previous findings , we found that Vav 2 can act as a potent GEF for Cdc 42 , Rac 1 , and RhoA in vitro . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| In contrast , GEF activity toward Rac 1 remained latent , implying that ACK 1 does not represent a tyrosine kinase that acts downstream of G protein coupled receptors . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| The Rac 1 and RhoG specific GEF domain of Trio targets filamin to remodel cytoskeletal actin . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| The GEF domain displayed selective activity on Rac 1 related GTPases . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Both Vav 1 and Tec , however , were capable of independent engagement of a bipartite intracellular domain of the PRLR : Deletion mapping studies confined this interaction to residues 323 to 527 of the intracellular domain of the PRLR : Furthermore , Tec enhanced the GEF activity of Vav 1 as evidenced by an increase in GTP bound Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| For example , Trio , GEF H 1 , and Tiam 1 are a subset of GEFs that specifically activate Rac 1 but not the closely related Cdc 42 . ^^^ The Rac 1 specificity of these GEFs appears to be governed by Rac 1 GEF binding interaction . ^^^ To understand the detailed mechanism underlying the GEF specificity issue , we have analyzed a panel of chimeras made between Rac 1 and Cdc 42 and examined a series of point mutants of Rac 1 made at the switch 1 , switch 2 , and beta ( 2 ) / beta ( 3 ) regions for their ability to interact with and to be activated by the GEFs . ^^^ The results reveal that Rac 1 residues of both the switch 1 and switch 2 regions are involved in GEF docking and GEF mediated nucleotide disruption , because mutation of Asp ( 38 ) , Asn ( 39 ) , Gln ( 61 ) , Tyr ( 64 ) , or Arg ( 66 ) / Leu ( 67 ) into Ala results in the loss of GEF binding , whereas mutation at Tyr ( 32 ) , Asp ( 65 ) , or Leu ( 70 ) / Ser ( 71 ) leads to the loss of GEF catalysis while retaining the binding capability . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Expression of the first GEF domain alone results in drastically shortened axons and excessive growth cones , mediated by Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Here , we utilized the crystal structure of the DH / PH domains of the Rac specific GEF Tiam 1 in complex with Rac 1 to determine the structural elements of Rac 1 that regulate the specificity of this interaction . ^^^ Our findings identified unique GEF specificity determinants in Rac 1 and provide important insights into the mechanism of DH / PH selection of GTPase targets . . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Activation of endogenous Rac 1 by expression of constitutively active Rac guanine nucleotide exchange factor ( GEF ) derivatives was sufficient to induce high level NADPH oxidase activity in COS ( phox ) cells . ^^^ The constitutively active form of the hematopoietic specific GEF , Vav 1 , was the most effective at activating superoxide production , despite detection of higher levels of Rac 1 GTP upon expression of constitutively active Vav 2 or Tiam 1 derivatives . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the association of DOCK 2 and Vav , the guanine nucleotide exchanging factor ( GEF ) for Rac 1 , was demonstrated in Jurkat cells . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| An assay for GEF activity revealed that the var . 2 exhibits decreased GEF activity towards Cdc 42 , var . 3 exhibits a weak but significant activity toward Rac 1 and Cdc 42 , var . 4 exhibits significant activity toward RhoA and Cdc 42 , while var . 1 exhibits no activity toward RhoA , Rac 1 , or Cdc 42 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Indeed Vav 1 is a GEF for Rac 1 , Rac 2 and RhoG , and it is activated following tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| FERM domain including Rho GEF ( FIR ) belongs to Dbl family of guanine nucleotide exchange factors and specifically activates biochemical pathways specific for Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that IBP , a homologue of SWAP 70 , is a novel GEF for Rac 1 and Cdc 42 in T lymphocytes , which is recruited to the immunological synapse upon engagement of the antigen receptor . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| FERM domain including Rho GEF ( FIR ) is one of the guanine nucleotide exchange factors for Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Both wild type and mutant p 85 betaPIX displaying negative GEF activity ( L238R / L239S ) are similarly recruited to growth cones , suggesting that Rac 1 activation is not essential for translocation of the PIX complex ( PAK 2 p85 betaPIX Rac 1 ) . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Based on the available structural information , we have identified the highly conserved amino acid pairing of Asn ( 1406 ) Trio Asp ( 65 ) Rac 1 of the GEF Rho GTPase interaction as the critical catalytic machinery required for the Rac 1 GDP / GTP exchange reaction . ^^^ The N1406A / D1407A mutant of Trio acted dominant negatively in vitro by retaining Rac 1 binding activity but losing GEF catalytic activity and competitively inhibited Rac 1 activation by wild type Trio . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Salmonella enterica invasion of host cells requires the reversible activation of the Rho family GTPases Cdc 42 and Rac 1 by the bacterially encoded GEF SopE and the GAP SptP , which exert their function at different times during infection and are delivered into host cells by a type 3 secretion system . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| The chemical compound NSC 23766 was identified by a structure based virtual screening of compounds that fit into a surface groove of Rac 1 known to be critical for GEF specification . ^^^ In vitro it could effectively inhibit Rac 1 binding and activation by the Rac specific GEF Trio or Tiam 1 in a dose dependent manner without interfering with the closely related Cdc 42 or RhoA binding or activation by their respective GEFs or with Rac 1 interaction with BcrGAP or effector PAK 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Although total Rac 1 protein was not changed in KO VSMCs , the level of the Rac guanine exchange factor ( GEF ) , Sos 1 , was decreased . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Cdc 42 activated through c Src then enhanced the GEF activity of tyrosine phosphorylated Vav 2 on Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Here it is shown that L 1 clustering activates the guanine nucleotide exchange factor ( GEF ) Vav 2 and the Rac 1 effector p 21 associated kinase 1 ( Pak 1 ) . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| In this work we have characterized the structural and biological determinants for cytoskeletal regulation and cell transformation by the neuroepithelioma transforming gene 1 ( NET 1 ) , which is a GEF specific for RhoA , but not Cdc 42 or Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We show that the binding of activated Cdc 42 to the Cool 2 dimer markedly enhances its ability to associate with GDP bound Rac 1 , resulting in a significant activation of Rac GEF activity . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Mechanistically , the effects of Vav 1 require its GEF activity and the activation of Rac 1 , PAK 1 , and NF kappaB and involve cyclin D 1 upregulation . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Kalirin is a large neuronal dual Rho GEF that activates Rac 1 , RhoA , and RhoG via its two Rho GEF domains . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of ephexin 1 enhances ephexin 1 ' s GEF activity toward RhoA while not altering its activity toward Rac 1 or Cdc 42 , thus changing the balance of GTPase activities . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Here we report that TrioN , a Dbl family GEF , activates Rac 1 by facilitating GTP binding to , as well as stimulating GDP dissociation from , Rac 1 . ^^^ The TrioN stimulated nucleotide exchange may undergo a novel two nucleotide one G protein intermediate involving two cryptic subsites on Rac 1 induced by the GEF , with one subsite contributing to the recognition of the beta / gamma phosphates of the incoming GTP and another to the binding of the guanine base of the leaving GDP . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Moreover , the beta3beta4 loop of the PH domain of P Rex 2 is the determinant for Rac 1 recognition , as substitution of the beta3beta4 loop of the PH domain of Dbs ( a RhoA and Cdc 42 specific GEF ) with that of P Rex 2 confers Rac 1 specific binding capability to the PH domain of Dbs . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| As expected , Kal GEF 1 , an efficient GEF for Rac 1 and RhoG , induced the formation of lamellipodia resembling those induced by active Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| In this study , we characterized a mouse guanine nucleotide exchange factor ( GEF ) P Rex2b for its regulation by Gbetagamma and PI3K and its role in S1P induced Rac 1 activation and cell migration in ECs . ^^^ Together with the finding that co expression of Gbetagamma and / or PI3K ( AC ) increased the levels of active Rac 1 , we conclude that P Rex2b is a Rac GEF that can be regulated by Gbetagamma and PI3K . ^^^ Therefore , P Rex2b is a physiologically significant Rac 1 GEF that has an important role in the regulation of EC migration . . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting from S1P treated EC revealed S1P mediated rapid recruitment ( 1 microM , 5 min ) to CEMs of the S1P receptors S1P1 and S1P3 , p 110 PI3 kinase alpha and beta catalytic subunits , the Rac 1 GEF , Tiam 1 , and alpha actinin isoforms 1 and 4 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| We found that overexpression of the Rac 1 guanine nucleotide exchange factor ( GEF ) Tiam 1 markedly enhanced Rac 1 activity , whereas a dominant negative Tiam 1 mutant significantly attenuated S1P mediated Rac 1 activation . ^^^ Taken together , these studies identify G protein betagamma subunits , Src kinase and the GEF Tiam 1 as upstream modulators of S1P mediated Rac 1 activation , and establish a central role for Rac 1 in S1P mediated activation of PI 3 kinase / Akt / endothelial nitric oxide synthase signaling in vascular endothelial cells . . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| In fibroblast cells , NSC 23766 inhibited Rac 1 GTP loading without affecting Cdc 42 or RhoA activity and suppressed cell proliferation induced by a Rac GEF Tiam 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| The presence of both Rac 1 and Rab 5 GEF activities makes Alsin a unique dual exchange factor that may couple endocytosis ( via Rab 5 activation ) to cytoskeletal modulation ( via Rac 1 activation ) . . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| The Ras GRF 1 exchange factor has regulated guanine nucleotide exchange factor ( GEF ) activity for H Ras and Rac 1 through separate domains . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| The Son of Sevenless 1 protein ( sos 1 ) is a guanine nucleotide exchange factor ( GEF ) for either the ras or rac 1 GTPase . ^^^ We show that p66shc , an adaptor protein that promotes oxidative stress , increases the rac 1 specific GEF activity of sos 1 , resulting in rac 1 activation . ^^^ P66shc induced dissociation of sos 1 from grb 2 , formation of the sos 1 eps8 e3b1 complex , rac 1 specific GEF activity of sos 1 , rac 1 activation , and oxidative stress are also mediated by the PPLP motif in the CH 2 domain . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| The Rac specific GEF ( guanine nucleotide exchange factor ) Tiam 1 ( T lymphoma invasion and metastasis 1 ) regulates migration , cell matrix and cell cell adhesion by modulating the actin cytoskeleton through the GTPase , Rac 1 . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| In fibroblast cells NSC 23766 inhibited Rac 1 GTP loading without affecting Cdc 42 or RhoA activity and suppressed the Rac GEF , Tiam 1 , and oncogenic Ras induced cell growth and transformation . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| Indeed , EspG and Orf 3 induced stress fiber formation was inhibited by the expression of dominant negative forms of GEF H 1 and RhoA , but not of Rac 1 and Cdc 42 , and by treatment with a ROCK inhibitor . ^^^ |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92974 and P63000 |
Pubmed |
SVM Score :0.0 |
| NA |
|