| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Here we report the identification of the Cdk activating kinase ( Cak ) complex ( Cdk 7 and cyclin H ) as a component of TFIIH after extensive purification of TFIIH by chromatography . ^^^ Cak is present in at least two distinct complexes , TFIIH and a smaller complex that is unable to phosphorylate RNAPII in the preinitiation complex . ^^^ We find that affinity purified antibodies directed against cyclin H inhibit TFIIH dependent transcription and that both cyclin H and Cdk 7 antibodies inhibit phosphorylation of the CTD of the largest subunit of the RNAPII in the preinitiation complex . ^^^ Cdk activating kinase complex is a component of human transcription factor TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| We report here that two polypeptides of the purified mammalian TFIIH are the MO15 / Cdk7 kinase and cyclin H subunits of the Cdk activating kinase Cak , previously identified as a positive regulator of Cdc 2 and Cdk 2 . ^^^ TFIIH and Cak preparations are each capable of phosphorylating recombinant CTD and recombinant Cdk 2 proteins . ^^^ The presence of Cak in TFIIH indicates that Cak may have roles in transcriptional regulation and in cell cycle control . . ^^^ Association of Cdk activating kinase subunits with transcription factor TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| We show that human CAK possesses the CTD kinase activity characteristic of TFIIH . . ^^^ Relationship of CDK activating kinase and RNA polymerase 2 CTD kinase TFIIH / TFIIK . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Mammalian CDK 7 is a protein kinase identified as the catalytic subunit of cyclin dependent kinase ( CDK ) activating kinase and as an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair . ^^^ One class of proteins present in CDK 7 immunocomplexes as a minor fraction contains components of the TFIIH transcription complex such as p 62 and p89ERCC3 , whereas the other fraction contains four polypeptides ( p 35 , p37Cyclin H , p 75 , and p 95 ) that are stoichiometrically associated with CDK 7 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| MAT 1 , cdk 7 and cyclin H form a kinase complex which is UV light sensitive upon association with TFIIH . ^^^ MAT 1 , cyclin H and cdk 7 are part of TFIIH , a class 2 transcription factor which possesses numerous subunits of which several have been shown to be involved in processes other than transcription . ^^^ MAT 1 , cyclin H and cdk 7 exist as a ternary complex either free or associated with TFIIH from which the latter can be dissociated at high salt concentration . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Isolation and characterization of two human transcription factor IIH ( TFIIH ) related complexes : ERCC2 / CAK and TFIIH . ^^^ Recent studies have shown that TFIIH copurifies with the cyclin dependent kinase ( cdk ) activating kinase complex ( CAK ) that includes cdk 7 , cyclin H , and p36 / MAT1 . ^^^ Here we report the isolation of two TFIIH related complexes : TFIIH * and ERCC2 / CAK . ^^^ TFIIH * consists of a subset of the TFIIH complex proteins including ERCC 3 ( XPB ) , p 62 , p 44 , p 41 , and p 34 but is devoid of detectable levels of ERCC 2 ( XPD ) and CAK . ^^^ This TFIIH * dependent transcription reaction was stimulated by ERCC2 / CAK . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Here we report the isolation and characterization of three distinct CAK containing complexes from HeLa nuclear extracts : CAK , a novel CAK ERCC 2 complex , and TFIIH . ^^^ CAK ERCC 2 can efficiently associate with core TFIIH to reconstitute holo TFIIH transcription activity . ^^^ We present evidence proposing a critical role for ERCC 2 in mediating the association of CAK with core TFIIH subunits . . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Subsequently , both CDK 7 and its partner , cyclin H , were found to be associated with the general transcription factor TFIIH , suggesting additional roles for CDK 7 in transcription and DNA repair . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| CAK in TFIIH : crucial connection or confounding coincidence . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| It has been suggested that the stimulation of transcript elongation by conventional DNA binding activators may involve phosphorylation of the carboxy terminal domain ( CTD ) of Pol 2 by the transcription factor TFIIH through the associated CAK kinase . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Substrate specificity of the cdk activating kinase ( CAK ) is altered upon association with TFIIH . ^^^ Three subunits of TFIIH , cdk 7 , cyclin H and MAT 1 , form a ternary complex , cdk activating kinase ( CAK ) , found either on its own or as part of TFIIH . ^^^ Using these fractions , we demonstrate that TFIIH lacking the CAK subcomplex completely recovers its transcriptional activity in the presence of free CAK . ^^^ Furthermore , studies examining the interactions between TFIIH subunits provide evidence that CAK is integrated within TFIIH via XPB and XPD . . ^^^ TFIIH was resolved into four subcomplexes : the kinase complex composed of cdk 7 , cyclin H and MAT 1 ; the core TFIIH which contains XPB , p 62 , p 52 , p 44 and p 34 ; and two other subcomplexes in which XPD is found associated with either the kinase complex or with the core TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| This tripartite CAK occurs in a free form and in association with ' core ' TFIIH , which functions in both pol 2 transcription and DNA repair . ^^^ The substrate specificities of natural TFIIH and free CAK were also compared . ^^^ TFIIH had a strong preference for the CTD over CDK 2 relative to free CAK . ^^^ TFIIH , but not free CAK , could efficiently hyperphosphorylate the CTD . ^^^ In the context of TFIIH , the kinase also acquired specificity for the general transcription factors TFIIE and TFIIF which were not recognized by free CAK . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Both free CAK ( cdk 7 , cyclin H , MAT 1 ) and the CAK containing general transcription factor TFIIH phosphorylated Ser 77 in vitro . ^^^ Furthermore RAR alpha bound free CAK and purified TFIIH in vitro , and RAR alpha TFIIH complexes could be isolated from HeLa nuclear extracts . ^^^ Stimulation of RAR alpha activation function AF 1 through binding to the general transcription factor TFIIH and phosphorylation by CDK 7 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The deduced amino acid sequence of Tfb 2 is similar to that of the 52 kDa subunit of human TFIIH , while Tfb 3 is identified as a RING finger protein homologous to the 36 kDa subunit of murine CAK ( cyclin dependent kinase activating kinase ) and to the 32 kDa subunit of human TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The cdk 7 cyclin H MAT 1 complex associated with TFIIH is localized in coiled bodies . ^^^ TFIIH is composed of eight or nine subunits and we show that at least four of them , namely cdk 7 , cyclin H , MAT 1 , and p 62 are localized in the coiled body , a distinct subnuclear structure that is transcription dependent and highly enriched in small nuclear ribonucleoproteins . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| In budding yeast , the closest homologs of cdk 7 and cyclin H , KIN 28 and CCL 1 , respectively , also associate with TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrate that Tat binds directly to the cyclin dependent kinase 7 ( CDK 7 ) , which leads to productive interactions between Tat and the CDK activating kinase ( CAK ) complex and between Tat and TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| MAT 1 is known to be required for the assembly of cyclin dependent kinase ( CDK ) activating kinase ( CAK ) , which is functionally associated with the general transcription factor IIH ( TFIIH ) . ^^^ These results suggest ( 1 ) that interactions between POU domains and MAT 1 can target CAK to Oct factors and result in their phosphorylation , ( 2 ) that MAT 1 not only functions as a CAK assembly factor but also acts to alter the spectrum of CAK substrates , and ( 3 ) that a POU MAT 1 interaction may play a role in the recruitment of TFIIH to the preinitiation complex or in subsequent initiation and elongation reactions . . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Furthermore Kin 28 , the budding yeast Cdk 7 homolog , functions not as a CAK but as the catalytic subunit of TFIIH . ^^^ Vertebrate Cdk 7 is also known to be part of TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The CDK 7 cyclin H MAT 1 complex is tightly associated with a multiprotein complex TFIIH , which plays a dual role in transcription and DNA repair . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Repression of TFIIH transcriptional activity and TFIIH associated cdk 7 kinase activity at mitosis . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| General transcription factor TFIIH , which contains CDK 7 , phosphorylates the CTD in vitro . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| New evidence now suggests that cdk 7 is also involved in the processes of transcription initiation and DNA repair , associating with the general transcription factor TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The CDK activating kinase in TFIIH was unable to activate the CTD kinase activity of P TEFb . . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| TFIIH , a multisubunit complex was shown to be involved in several biological fundamental mechanisms of the cell : transcription , nucleotide excision repair and cell cycle regulation . p 62 is one of the six subunits that constitutes the core of TFIIH versus the holoenzyme , which contains , in addition , the ternary kinase CAK complex . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| This phosphorylation is resistant to DRB treatment , suggesting that not only phosphorylation but also transcription of heat shock genes is DRB resistant and that CDK 7 in heat shock cells is not associated with TFIIH . . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Human CAK has been identified as a p 40 ( MO 15 ) / cyclin H / MAT1 complex that also functions as part of transcription factor IIH ( TFIIH ) where it phosphorylates multiple transcriptional components including the C terminal domain ( CTD ) of the large subunit of RNA polymerase 2 . ^^^ In contrast , CAK from budding yeast consists of a single polypeptide ( Cak1p ) , is not a component of TFIIH , and lacks CTD kinase activity . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The molecular mechanism of mitotic inhibition of TFIIH is mediated by phosphorylation of CDK 7 . ^^^ A member of the cyclin dependent kinase family , CDK 7 , is the kinase subunit of TFIIH . ^^^ Here we show that in mitosis the CDK 7 subunit of TFIIH and the largest subunit of RNAPII become hyperphosphorylated . ^^^ MPF induced phosphorylation of CDK 7 results in inhibition of the TFIIH associated kinase and transcription activities . ^^^ Negative and positive regulation of TFIIH requires phosphorylation within the T loop of CDK 7 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| A mammalian RNA polymerase 2 complex containing all of the components required for promoter specific transcription initiation can be isolated by immunopurification with a monoclonal antibody directed against the cyclin dependent kinase CDK 7 , a subunit of the general transcription factor TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Designing mutated recombinant subunits , we show that the XPB helicase is absolutely required for transcription to open the promoter around the start site whereas the XPD helicase , which is dispensable , stimulates transcription and allows the CAK complex to be anchored to TFIIH . ^^^ Reconstitution of the transcription factor TFIIH : assignment of functions for the three enzymatic subunits , XPB , XPD , and cdk 7 . ^^^ Having succeeded in reconstituting a functionally active TFIIH from baculovirus recombinant polypeptides , we were able to analyze the role of XPB , XPD , and cdk 7 subunits in the transcription reaction . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Recent reports have shown that Tat can also interact with the multisubunit transcription factor TFIIH complex and increase the phosphorylation of CTD by the Cdk activating kinase ( CAK ) complex associated with the core TFIIH . ^^^ Furthermore , immunodepletion of CAK under high salt conditions , which allow CAK to be dissociated from core TFIIH , has no effect on either basal HIV 1 transcription or Tat activation of polymerase elongation in vitro . ^^^ Therefore , unlike the P TEFb kinase activity that is essential for Tat activation of HIV 1 transcriptional elongation , the CAK kinase associated with TFIIH appears to be dispensable for Tat function . . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The catalytic subunit of mammalian CAK , MO15 / Cdk7 , also functions as a subunit of the general transcription factor TFIIH . ^^^ However , these functions are split in budding yeast , where Kin28p functions as the kinase subunit of TFIIH and Cak1p functions as a CAK . ^^^ These findings indicate that although MO 15 and Cak1p constitute different forms of CAK , both control the cell cycle and the phosphorylation of the C terminal domain of the large subunit of RNA polymerase 2 by TFIIH . . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| TFIIH possesses three known ATP dependent activities : a 3 ' > 5 ' DNA helicase catalyzed by its XPB subunit , a 5 ' > 3 ' DNA helicase catalyzed by its XPD subunit , and a carboxyl terminal domain ( CTD ) kinase activity catalyzed by its CDK 7 subunit . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| To provide an explanation of some clinical features observed within rare xeroderma pigmentosum ( XP ) patients and to further define the role of XPB , XPD , and cdk 7 , the three enzymatic subunits of TFIIH , in the transcription reaction , we have examined two defined enzymatic steps : phosphodiester bond formation and promoter escape . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Nucleotide excision repair of DNA with recombinant human proteins : definition of the minimal set of factors , active forms of TFIIH , and modulation by CAK . ^^^ The most complex and difficult to analyze factor is TFIIH , which has a 6 subunit core ( XPB , XPD , p 44 , p 34 , p 52 , p 62 ) and a 3 subunit kinase ( CAK ) . ^^^ Recombinant TFIIH also functioned in repair , both a 6 and a 9 subunit form containing CAK . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Co transfection of cdk activating kinase ( CAK ) , the kinase moiety of TFIIH , enhanced AR mediated transcription in a ligand dependent manner in human prostate cancer PC 3 and LNCaP cells , and in a ligand independent manner in human prostate cancer DU 145 cells . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Cdk 7 is also found in the transcription factor complex TFIIH , suggesting that it participates in vivo in the control of RNA polymerase 2 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| RARgamma is more efficiently phosphorylated by TFIIH than by CAK and interacts not only with cdk 7 but also with several additional subunits of TFIIH . ^^^ TFIIH interacts with the retinoic acid receptor gamma and phosphorylates its AF 1 activating domain through cdk 7 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The transcription / DNA repair factor TFIIH may be resolved into at least two subcomplexes : the core TFIIH and the cdk activating kinase ( CAK ) complex . ^^^ The median portion of MAT 1 , which contains a coiled coil motif , allows the binding of CAK to the TFIIH core through interactions with both XPD and XPB helicases . ^^^ Distinct regions of MAT 1 regulate cdk 7 kinase and TFIIH transcription activities . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Our results demonstrate that cyclin dependent kinase 7 ( CDK 7 ) ( TFIIH ) and CDK 9 ( P TEFb ) both associate with the HIV 1 preinitiation complex . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We prepared TFIIH and two Cdk 7 containing kinase complexes , Cdk7 / Cyclin H and CAK ( Cdk7 / Cyclin H / MAT1 ) . ^^^ The kinase activity of TFIIH displayed stronger substrate preference for Cdk 4 than did CAK . ^^^ In addition , TFIIH phosphorylation of PolII was stimulated by TFIIE both in solution and during preinitiation complex formation , whereas Cdk7 / Cyclin H and CAK phosphorylation of PolII was not . ^^^ In combination with other general transcription factors , TFIIH , but not Cdk7 / CycH or CAK , promoted transcription on a linear DNA template . ^^^ TFIIH consists of nine subunits , and one of them , Cdk 7 , possesses kinase activity . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Activation of estrogen receptor alpha by S 118 phosphorylation involves a ligand dependent interaction with TFIIH and participation of CDK 7 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Cyclin dependent kinase 7 ( Cdk 7 ) forms a trimeric complex with cyclin H and Mat 1 to form the mammalian Cdk activating kinase , CAK , as well as a part of the basal transcription factor TFIIH , where Cdk 7 phosphorylates the C terminal domain ( CTD ) of the large subunit of RNA polymerase 2 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| TFIIH contains a CDK 7 kinase subunit and phosphorylates the CTD . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| TFIIH is a multifunctional RNA polymerase 2 general initiation factor that includes two DNA helicases encoded by the Xeroderma pigmentosum complementation group B ( XPB ) and D ( XPD ) genes and a cyclin dependent protein kinase encoded by the CDK 7 gene . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| As biochemists , we have characterized each component of TFIIH , three of which are XPB and XPD helicases and cdk 7 , a cyclin dependent kinase . ^^^ We now know how the XPB helicase opens the promoter region for RNA synthesis and that one of the roles of XPD helicase is to anchor the cdk 7 kinase to the core TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| When the ternary Cdk activating kinase ( CAK ) complex composed of Cdk 7 , cyclin H , and MAT 1 was used as bait , the xeroderma pigmentosum ( XP ) D helicase of transcription factor IIH ( TFIIH ) , among other proteins , was identified as an interacting partner . ^^^ Using immunoprecipitates from cells coinfected with baculoviruses , we further validated the bridging function of XPD , which anchors CAK to the core TFIIH . ^^^ XPD and / or the role of CAK within TFIIH and , consequently , explaining the variety of the XP phenotypes . . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| However , with cyclin dependent kinase ( Cdk 2 ) and TFIIH complex associated kinase CAK , the phosphorylation of p 53 was increased at later time points ( 25 h ) . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Loss of Hnt 1 enzyme activity also leads to hypersensitivity to mutations in Ccl 1 , Tfb 3 , and Kin 28 , which constitute the TFIIK kinase subcomplex of general transcription factor TFIIH and to mutations in Cak 1 , which phosphorylates Kin 28 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Together with TFIIH subunits cyclin H and Mat 1 , Cdk 7 kinase is also found in a trimer complex known as Cdk activating kinase ( CAK ) . ^^^ The catalytic kinase subunit of TFIIH is a member of the cyclin dependent kinase ( Cdk ) family , designated Kin 28 in Saccharomyces cerevisiae and Cdk 7 in higher eukaryotes . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Thus , we demonstrate that the cdk 7 kinase of TFIIH phosphorylates the nuclear receptor , then allowing ligand dependent control of the activation of the hormone responsive genes . . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| CDK 7 is the kinase subunit of the general transcription factor TFIIH that phosphorylates the C terminal domain ( CTD ) of RNA polymerase 2 , and has been shown to be broadly required for transcription in Saccharomyces cerevisiae . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The kinase subunits of TFIIH in animal cells , Cdk 7 , cyclin H , and MAT 1 , were independently isolated as an activity termed CAK ( Cdk activating kinase ) , which phosphorylates and activates cell cycle kinases . ^^^ However , CAK activity of TFIIH subunits could not be demonstrated in budding yeast . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The mammalian Mat 1 protein has been implicated in cell cycle regulation as part of the Cdk activating kinase ( CAK ) , and in regulation of transcription as a subunit of transcription factor TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| A CDK 7 mutant defective in kinase activity for the C terminal tail of RNA polymerase 2 , which can not form a functional TFIIH complex , did not enhance Vpr coactivator activity . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| MAT 1 is an assembly / targeting factor for cyclin dependent kinase activating kinase ( CAK ) , which has been shown to functionally interact with general transcriptional factor TFIIH , a known inducer of ER transactivation . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Often , phosphorylation of NRs by kinases that are associated with general transcription factors ( e . g . cdk 7 within TFIIH ) , or activated in response to a variety of signals ( MAPKs , Akt , PKA , PKC ) , facilitates the recruitment of coactivators or of components of the transcription machinery and , therefore , cooperates with the ligand to enhance transcription activation . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The antibody study indicated that they thus altered the conformation of one side of TFIIH , consisting of p 44 , XPD , and Cdk activating kinase subunits , that is essential for the transition stage . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| General transcription factor IIH ( TFIIH ) consists of nine subunits : cyclin dependent kinase 7 ( Cdk 7 ) , cyclin H and MAT 1 ( forming the Cdk activating kinase or CAK complex ) , the two helicases Xpb / Hay and Xpd , and p 34 , p 44 , p 52 and p 62 ( refs 1 3 ) . ^^^ Here we show that the Drosophila TFIIH component Xpd negatively regulates the cell cycle function of Cdk 7 , the CAK activity . ^^^ As the kinase subunit of TFIIH , Cdk 7 participates in basal transcription by phosphorylating the carboxy terminal domain of the largest subunit of RNA polymerase 2 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| In transcription , the helicase activity of TFIIH functions to melt promoter DNA ; however , the in vivo function of the Cdk 7 kinase subunit of TFIIH , which has been hypothesized to be involved in RNA polymerase 2 ( Pol 2 ) phosphorylation , is not clearly understood . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| XPB is part of the core of TFIIH and has a central role in transcription , whereas XPD connects the core to the CAK subcomplex , and can tolerate many different mutations . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Besides MAPK , cyclin dependent kinase 7 ( Cdk 7 ) in the TFIIH complex has also been found to potentiate hERalpha transactivation in vitro through Ser ( 118 ) phosphorylation . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Contrary to other nuclear receptors , VDR , which lacks a functional A / B domain , is not phosphorylated and consequently not regulated by the cdk 7 kinase of TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| CDK 7 also assists in the regulation of transcription as part of the transcription factor TFIIH complex . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Impairment of the TFIIH associated CDK activating kinase selectively affects cell cycle regulated gene expression in fission yeast . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Together with six other subunits , CAK is also part of the general transcription factor TFIIH where it is involved in promoter clearance and progression of transcription from the preinitiation to the initiation stage . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| In addition , other substrates of the CAK complex have been identified when CAK is assembled with the TFIIH core proteins , thereby regulating transcription and nucleotide excision repair . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The defect in transactivation by PPARs is likely attributable to their weaker phosphorylation by the cdk 7 kinase of TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The N terminal AF 1 domain of RARalpha is phosphorylated at S 77 by the cyclin dependent kinase ( cdk ) activating kinase ( CAK ) subcomplex ( cdk7 / cyclin H / MAT1 ) of the general transcription factor TFIIH . ^^^ We propose a model in which the structural constraints of this region control the architecture of the RAR / RXR / TFIIH complex and therefore the efficiency of RARalpha phosphorylation by cdk 7 . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| In metazoans , cyclin dependent kinase 7 ( CDK 7 ) has essential roles in both the cell division cycle and transcription , as a CDK activating kinase ( CAK ) and as a component of the general transcription factor TFIIH , respectively . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| The other CAK Mcs 6 , the kinase component of transcription factor IIH ( TFIIH ) cannot activate Cdk 9 . ^^^ Cyclin dependent kinase 9 ( Cdk 9 ) of fission yeast is activated by the CDK activating kinase Csk 1 , overlaps functionally with the TFIIH associated kinase Mcs 6 , and associates with the mRNA cap methyltransferase Pcm 1 in vivo . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| Similarly , recombinant p 65 activates HIV transcription in vitro and stimulates phosphorylation of the RNAP 2 CTD by the CDK 7 kinase module of TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P50613 |
Pubmed |
SVM Score :0.0 |
| NA |
|