| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The basal promoter , a template containing a TATA box devoid of upstream regulatory sequences , has been used to identify and characterize the factors which , together with RNA polymerase 2 , govern transcription in mammalian systems : TFIIA , TFIIB , TFIID , TFIIE , TFIIF , TFIIG , TFIIH , and TFIIJ . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| These factors , TFIIH and TFIIJ , were required together with the previously described general factors ( TFIIA , TFIIB , TFIID , TFIIE , and TFIIF ) and RNA polymerase 2 for transcription of different class 2 genes . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The immunoprecipitate includes RNA polymerase 2 , TFIID , TFIIB , TFIIH , TFIIF , and TFIIE , but is devoid of transcriptional activator proteins , such as HNF 1 , HNF 4 , and C / EBP alpha . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| During this transition , TFIID remains promoter bound whereas TFIIB , TFIIE , TFIIF , and TFIIH are released . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Interaction of HBx with p 53 did not prevent the activation domain of p 53 from binding two general initiation factors , the TATA box binding protein subunit of TFIID and the p 62 subunit of TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Although little is known about their function , recent data demonstrate the ability of these proteins , previously called class 2 transcription factors , to participate in other reactions : TBP , the TATA box binding factor , is involved in class 1 and 3 transcription , while TFIIH has been shown to possess components that are involved in the DNA repair mechanism . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The mechanism by which site specific transcription factors increase specific initiation at polymerase 2 dependent promoters is thought to occur via recruitment and stabilization of components that form the initiation complex , i . e . , TFIID , TFIIA , TFIIB , TFIIE , TFIIG , TFIIH , TFIIJ and pol 2 . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Using fluorescence in situ hybridization ( FISH ) , we show here that the genes encoding the TATA box binding protein ( TBP ) , TFIIB , TFIIE alpha , TFIIE beta , RAP 30 , RAP 74 and the 62 kDa subunit , of TFIIH are located at the human chromosomal bands 6q26 27 , 1p21 22 , 3q21 24 , 8p12 , 13q14 , 19p13 . 3 and 11p14 15 . 1 , respectively . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| We also show that TFIIH specifically phosphorylates three general transcription factors , human TFIID tau ( TBP ) , TFIIE alpha and TFIIF alpha ( RAP 74 ) . . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| First , we observe that synthesis of the first phosphodiester bond of transcripts initiated at the adenovirus 2 major late promoter depends strongly on ATP , TFIIE , and TFIIH in a transcription system reconstituted with RNA polymerase 2 , TFIIH , and recombinant TBP , TFIIB , TFIIE , and TFIIF . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Here we report that Gal11p stimulates basal transcription in a reconstituted transcription system composed of recombinant or highly purified transcription factors , TFIIB , TFIIE , TFIIF , TFIIH , and TATA box binding protein and core RNA polymerase 2 . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The cDNAs encoding human basal transcription factors TBP , TAFII 55 and the p 62 subunit of TFIIH were individually cloned into these vectors and introduced into a HeLa derived cell line that constitutively expresses a tetracycline regulated transactivator ( tTA ) . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Instead , general initiation factors [ transcription factor ( TF ) IIB , TFIID , TFIIE , TFIIF , and TFIIH ] assemble on promoter DNA with polymerase 2 , creating a large multiprotein DNA complex that supports accurate initiation . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The Cdc73p Paf1p RNA polymerase 2 containing complex also includes Gal11p , and the general initiation factors TFIIB and TFIIF , but lacks TBP , TFIIH , and transcription elongation factor TFIIS as well as the Srbps . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| With the availability of the general initiation factors ( TFIIB , TFIID , TFIIE , TFIIF , and TFIIH ) , it is now possible to investigate aspects of the mechanism of eukaryotic messenger RNA synthesis in purified , reconstituted RNA polymerase 2 transcription systems . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| This holoenzyme contained nearstoichiometric amounts of all the general transcription factors , TFIIB , TFIID ( TBP + TAFIIs ) , TFIIE , TFIIF , and TFIIH , required to accurately initiate transcription in vitro at the adenovirus major late promoter . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| We have characterized interactions of the E2F1 activation domain with two general transcription factors , the TATA box binding protein ( TBP ) and TFIIH . ^^^ Modular organization of the E2F1 activation domain and its interaction with general transcription factors TBP and TFIIH . ^^^ We have demonstrated that recombinant Rb can compete with TBP and the p 62 subunit of TFIIH for binding to immobilized E2F1 . ^^^ We propose that competition between Rb and both TBP and TFIIH for binding to E2F1 is a mechanism by which Rb inhibits transactivation by E2F1 . . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Using filter binding as well as in vitro transcription challenge competition assays , we now show that the promoter recognition factor TATA box binding protein ( TBP ) / TFIID binds selectively to and is sequestered by cisplatin or UV damaged DNA , either alone or in the context of a larger protein complex including TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Here we show that PC 4 coactivator function , in contrast to basal ( activator independent ) transcription , is dependent both on TATA binding protein ( TBP ) associated factors ( TAFs ) in TFIID and on TFIIH . ^^^ Furthermore , TFIIH and TAFII 250 , the largest subunit of TFIID , can both phosphorylate PC 4 . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| For an activated promoter , targets of repression are TFIID and TFIIH , while for a basal promoter , TFIIH is the major target for mitotic inactivation of transcription . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The core promoter is the site for assembly of the transcription preinitiation complex , which includes RNA pol 2 and the general transcription fctors TBP , TFIIB , TFIIE , TFIIF , and TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| These studies suggest an alternative PIC assembly pathway in which TFIIE affects both TBP and TFIIH functions during initiation of RNA synthesis . . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Using transcription competition , nitrocellulose filter binding , and DNase 1 footprinting assays , we show that , although the general transcription factor TFIIH is able to target any kind of lesion which can be repaired by the nucleotide excision repair pathway , TATA binding protein ( TBP ) TFIID is more selective in damage recognition . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| In contrast , general transcription factors TFIIH and TFIIA play a significant role in TBP mediated activation , which can be detected in vitro with Gal 4 fusion proteins containing various transcriptional activation domains . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| By exploiting adenovirus 2 major late promoter derivatives that contain premelted transcriptional start sites and do not require TFIIF , TFIIE , or TFIIH for transcription initiation , we observe ( 1 ) that Elongin is capable of strongly stimulating the rate of synthesis of trinucleotide transcripts by a subcomplex of RNA polymerase 2 , TBP , and TFIIB and ( 2 ) that the ability of Elongin to stimulate synthesis of these short transcripts is substantially reduced by addition of TFIIF to transcription reactions . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Interestingly , repression of basal transcription by the positive cofactor PC 4 is alleviated by increasing amounts of TFIID , TFIIH , and pol 2 holoenzyme , suggesting that phosphorylation of PC 4 by these proteins may cause a conformational change in the structure of PC 4 that allows for preinitiation complex formation and initiation of transcription . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| These data imply that Archaea do not possess a functional homologue of transcription factor TFIIH , and that for the promoters studied , transcription is mediated by TATA box binding protein , transcription factor TFB , and RNA polymerase alone . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| However , additional amino acid substitutions for phenylalanine 429 demonstrated a strong correlation between activation of the dhfr promoter and binding of CBP , but not TFIIH or TBP . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The DNA wrapping model describes specific roles for general RNA polymerase 2 transcription factors ( TATA binding protein [ TBP ] , TFIIB , TFIIF , TFIIE , and TFIIH ) , provides a plausible explanation for preinitiation complex isomerization , suggests mechanisms underlying the synergy between transcriptional activators , and suggests an unforseen role for TBP associating factors in transcription . . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| TBP association depends on the Pol 2 holoenzyme component Srb 4 , but not on the Kin 28 subunit of the transcription factor TFIIH , even though both proteins are generally required for transcription . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The enzyme is also involved in transcriptional regulation through its ability to associate with other proteins in the TFIID , and possibly TFIIH , transcription complexes , and is implicated in the recognition of DNA lesions . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Our results show that a system consisting of purified recombinant TFIIA , TFIIB , TFIIE , TFIIF , CREB , and Tax , along with highly purified RNA polymerase 2 , affinity purified epitope tagged TFIID , and semipurified TFIIH , supports basal transcription of the HTLV 1 promoter but is not responsive to Tax . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| These studies led to the discovery of the class of general initiation factors ( TFIIB , TFIID , TFIIE , TFIIF , and TFIIH ) , which function in intimate association with RNA polymerase 2 and are required for selective binding of polymerase to its promoters , formation of the open complex , and synthesis of the first few phosphodiester bonds of nascent transcripts . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| While new atomic structures of different domains of general transcription factors , such as the double bromodomain of TAF 250 , have become available by means of 10 ray crystallography and NMR studies , more global pictures of multisubunit transcription complexes , such as TFIID , TFIIH or the yeast mediator , have now been obtained by electron microscopy and image reconstruction techniques . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Here we describe the isolation of a reinitiation intermediate that includes transcription factors TFIID , TFIIA , TFIIH , TFIIE and Mediator . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Interestingly , multiple basal transcription factors for RNA polymerase 2 , including TBP , TFIIB , TFIIH , and TFIIF , were found to be associated with MSS 1 . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Using the human basal transcription factors TFIID and TFIIH as examples , we show that pairwise coexpression of polypeptides in Escherichia coli can be used as a tool for the identification of specifically interacting subunits within multiprotein complexes . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The involvement of individual components , including TFIID , TFIIH , RNA polymerase 2 and xeroderma pigmentosum group A ( XPA ) , in the triplex mediated TCR process was demonstrated in HeLa nuclear extracts using immunodepletion assays . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Using order of addition and template challenge assays performed in a human cell free transcription system reconstituted with recombinant general transcription factors ( TFIIB , TBP , TFIIE , TFIIF ) , a recombinant general cofactor ( PC 4 ) , and highly purified epitope tagged multiprotein complexes ( TFIID , TFIIH , pol 2 ) , we demonstrate that when TBP is used as the TATA binding factor transcriptional activators such as Gal 4 VP16 and human papillomavirus E 2 mainly function by facilitating pol 2 entry to the promoter region . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| A mouse in vitro transcription system reconstituted from highly purified RNA polymerase 2 , TFIIH and recombinant TBP , TFIIB , TFIIE and TFIIF . ^^^ Unregulated transcription of protein encoding genes in vitro is dependent on 12 subunit core RNA polymerase 2 and five general transcription factors ; TATA binding protein ( TBP ) , transcription factor ( TF ) IIB , TFIIE , TFIIF , and TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| This model stipulates that wrapping of the promoter DNA around RNAPII and the general initiation factors TBP , TFIIB , TFIIE , TFIIF and TFIIH induces a torsional strain in the DNA double helix that facilitates strand separation and open complex formation . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| The measurements showed comparable amounts of RNA polymerase 2 , TFIIE , and TFIIF , lower levels of TBP and TFIIB , and still lower levels of Mediator and TFIIH . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| Here , it is shown that the APIS complex is physically associated with many general transcription factors , including components of yeast FACT ( Cdc68 / Pob3 ) , TFIID , TFIIH , and the RNA polymerase 2 holoenzyme . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| In this report , we describe a reconstituted in vitro transcription system that consists of the following preparations of factors from the yeast Schizosaccharomyces pombe : affinity purified RNAPII , TFIIH , and recombinant TBP , TFIIB , TFIIE and TFIIF . ^^^ RNA polymerase 2 ( RNAPII ) requires a set of general transcription factors TFIIA , TFIIB , TFIID , TFIIE , TFIIF and TFIIH to initiate transcription from a gene promoter in vitro . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| We have studied repair and transcription activities at 8 oxoG lesions with a reconstituted transcription system ( RTS ; RNA polymerase 2 , TBP , TFIIA , TFIIB , TFIIE , TFIIF and TFIIH ) , as well as in cellular extracts and in vivo . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| However , the dissection of this mechanism requires the addition of well characterized general transcription factors ; with the exception of TFIID and TFIIH , all others can easily be expressed in a recombinant form . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| We have analyzed an interaction of the general transcription complex RNA polymerase 2 proteins ( RNA polymerase 2 , factors TBP , TFIIB , TFIIF , TFIIE and TFIIH ) S . cerevisiae with the oligoribonucleotides . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| These studies indicate that the hsp 70 promoter is already occupied by TATA binding protein ( TBP ) and several TBP associated factors ( TAFs ) , TFIIB , TFIIF ( RAP 30 ) , TFIIH ( XPB ) , TBP free / TAF containg complex ( GCN 5 and TRRAP ) , and the Mediator complex subunit 13 before heat shock . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| In eukaryotes , the core promoter serves as a platform for the assembly of transcription preinitiation complex ( PIC ) that includes TFIIA , TFIIB , TFIID , TFIIE , TFIIF , TFIIH , and RNA polymerase 2 ( pol 2 ) , which function collectively to specify the transcription start site . ^^^ |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q92759 and P20226 |
Pubmed |
SVM Score :0.0 |
| NA |
|