Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The ERCC 3 protein has a dual function : it is a component of the transcription factor TFIIH and is an essential participant in the cellular nucleotide excision repair pathway . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Three unusual repair deficiencies associated with transcription factor BTF 2 ( TFIIH ) : evidence for the existence of a transcription syndrome . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The incision reaction was then reconstituted with the purified proteins RPA , XPA , TFIIH ( containing XPB and XPD ) , XPC , UV DDB , XPG , partially purified ERCC1 / XPF complex , and a factor designated IF 7 . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The p 89 ( ERCC 3 ) , p 80 ( ERCC 2 ) , and p 62 subunits of TFIIH were among the proteins retained by GST EBNA 2 . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The ERCC 3 protein was found to be part of a multiprotein complex ( TFIIH ) required for transcription initiation of most structural genes and for NER . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Protein affinity assays demonstrate that TFIIE binds directly to ERCC 3 , a DNA repair protein associated with TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
A protein kinase activity that phosphorylates the C terminal domain ( CTD ) of RNA polymerase 2 and is associated with the basal transcription repair factor TFIIH ( also called BTF 2 ) resides with MO 15 , a cyclin dependent protein kinase that was first found to be involved in cell cycle regulation . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The observation that the largest subunit of TFIIH is the excision repair protein XPB / ERCC3 ( ref . 1 ) , a helicase implicated in the human DNA repair disorders xeroderma pigmentosum ( XP ) and Cockayne ' s syndrome , suggests a functional link between transcription and DNA repair . ^^^ TFIIH is shown to complement three different cell extracts deficient in excision repair : XPB / ERCC3 , XPC and XPD / ERCC2 . ^^^ The complementation of XPB and XPD is a consequence of ERCC 3 and ERCC 2 being integral subunits of TFIIH , whereas complementation of XPC is due to an association of this polypeptide with TFIIH . ^^^ We found that the general transcription factor IIE negatively modulates the helicase activity of TFIIH through a direct interaction between TFIIE and the ERCC 3 subunit of TFIIH . . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Correction of xeroderma pigmentosum repair defect by basal transcription factor BTF 2 ( TFIIH ) . ^^^ The recent finding that its gene product is identical to the p 89 subunit of basal transcription factor BTF 2 ( TFIIH ) , opened the possibility that it is not directly involved in NER but that it regulates the transcription of one or more NER genes . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The human BTF 2 ( TFIIH ) transcription factor is a multisubunit protein involved in transcription initiation by RNA polymerase 2 ( B ) as well as in DNA repair . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
In addition , delta ' s human homolog , BTF 2 ( TFIIH ) , was recently shown to have an associated DNA helicase activity ( Schaeffer , L . , Roy , R . , Humbert , S . , Moncollin , V . , Vermeulen , W . , Hoeijmakers , J . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Phosphorylation of the heptapeptide repeats in the C terminal domain ( CTD ) of the largest subunit of RNA polymerase 2 has been widely proposed as an essential step in transcription initiation on the basis of findings indicating ( 1 ) that the CTDs of RNA polymerase 2 molecules actively engaged in transcription are highly phosphorylated ; ( 2 ) that polymerase molecules containing non phosphorylated CTDs preferentially enter the preinitiation complex where they are subsequently phosphorylated ; and ( 3 ) that essential initiation factors b from yeast , delta from rat , and BTF 2 ( TFIIH ) from human cells have closely associated CTD kinase activities . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Alternatively , HBx could affect p 53 binding to the TFIIH transcription nucleotide excision repair complex as HBx binds to the COOH terminus of p 53 and inhibits its binding to XPB or XPD . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
There are multiple protein protein contacts , involving two regions of p 53 and three subunits of TFIIH , ERCC 2 ( XPD ) , ERCC 3 ( XPB ) and p 62 . p 53 and its C terminus ( amino acids 320 393 ) inhibit both of the TFIIH helicases and in vitro transcription in the absence of TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Several proteins , including Rad 3 and Rad 25 ( Ssl 2 ) , are essential for nucleotide excision repair ( NER ) and function in the RNA polymerase 2 transcription initiation complex TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Reconstitution of TFIIH and requirement of its DNA helicase subunits , Rad 3 and Rad 25 , in the incision step of nucleotide excision repair . ^^^ Yeast TFIIH is composed of six subunits : Rad 3 , Rad 25 , TFB 1 , SSL 1 , p 55 , and p 38 . ^^^ In addition to TFIIH , we have purified a subassembly of the factor that lacks Rad 3 and Rad 25 and which we refer to as TFIIHi . ^^^ The NER efficacy of TFIIH is greatly diminished or abolished upon substitution of Rad 3 with the rad 3 Arg 48 mutant protein or Rad 25 with the rad 25 Arg 392 mutant protein , respectively , thus indicating a role of the Rad 3 and Rad 25 DNA helicase functions in the incision of damaged DNA . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
At least two of the subunits of TFIIH ( XPB and XPD proteins ) are implicated in the disease xeroderma pigmentosum ( XP ) . ^^^ We have exploited the availability of the cloned XPB , XPD , p 62 , p 44 , and p 34 genes ( all of which encode polypeptide subunits of TFIIH ) to examine interactions between in vitro translated polypeptides by co immunoprecipitation . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
A 3 ' > 5 ' XPB helicase defect in repair / transcription factor TFIIH of xeroderma pigmentosum group B affects both DNA repair and transcription . ^^^ XPB is a subunit of the basal transcription factor TFIIH , which is also involved in nucleotide excision repair ( NER ) and potentially in cell cycle regulation . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Because p 53 binds to the basal transcription repair complex TFIIH and modulates its DNA helicase activities , we hypothesized that TFIIH DNA helicases XPB and XPD are members of the p 53 mediated apoptotic pathway . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
TFIIH * consists of a subset of the TFIIH complex proteins including ERCC 3 ( XPB ) , p 62 , p 44 , p 41 , and p 34 but is devoid of detectable levels of ERCC 2 ( XPD ) and CAK . ^^^ The ERCC2 / CAK and TFIIH * complexes are each active in DNA repair as shown by their ability to complement extracts prepared from ERCC 2 ( XPD ) and ERCC 3 ( XPB ) deficient cells , respectively , in supporting the excision of DNA containing a cholesterol lesion . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The DNA helicases XPB and XPD , components of transcription factor TFIIH , have been implicated in a p 53 induced apoptotic pathway . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We have identified ERCC 3 and ERCC 2 DNA helicase subunits of holoenzyme TFIIH as targets of HBx interactions . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Besides XPD and TTDA , the XPB gene product is also part of TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
TFIIH was resolved into four subcomplexes : the kinase complex composed of cdk 7 , cyclin H and MAT 1 ; the core TFIIH which contains XPB , p 62 , p 52 , p 44 and p 34 ; and two other subcomplexes in which XPD is found associated with either the kinase complex or with the core TFIIH . ^^^ Furthermore , studies examining the interactions between TFIIH subunits provide evidence that CAK is integrated within TFIIH via XPB and XPD . . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The XPB subunit of repair / transcription factor TFIIH directly interacts with SUG 1 , a subunit of the 26S proteasome and putative transcription factor . ^^^ To search for proteins whose interaction with TFIIH subunits is disturbed by mutations in patients we used the yeast two hybrid system and report the isolation of a novel XPB interacting protein , SUG 1 . ^^^ The interaction was validated in vivo and in vitro in the following manner . ( 1 ) SUG 1 interacts with XPB but not with the other core TFIIH subunits in the two hybrid assay . ( 2 ) Physical interaction is observed in a baculovirus co expression system . ( 3 ) In fibroblasts under non overexpression conditions a portion of SUG 1 is bound to the TFIIH holocomplex as deduced from co purification , immunopurification and nickel chelate affinity chromatography using functional tagged TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The XPB and XPD proteins are subunits of RNA polymerase 2 ( RNAP 2 ) transcription factor IIH ( TFIIH ) . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Previously , p 53 was shown to interact with three components of transcription factor IIH ( TFIIH ) : excision repair cross complementing types 2 and 3 ( ERCC 2 and ERCC 3 ) and p 62 . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We investigated the mechanism of open complex formation and find that mutations in XPB or XPD , the DNA helicase subunits of the transcription and repair factor TFIIH , can completely prevent opening and dual incision in cell free extracts . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We conclude that the predominant active form of TFIIH is composed of nine subunits and that there is one molecule of XPB per TFIIH complex . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Of these , the XPB and XPD genes encode proteins that are subunits of a general transcription factor , TFIIH , involved in both nucleotide excision repair ( NER ) and initiation of mRNA transcription by RNA polymerase 2 . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
A procedure to immunoaffinity purify the human transcription factor IIH ( TFIIH ) was developed using a monoclonal antibody that recognizes an epitope in ERCC 3 ( XPB ) , the largest subunit of TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We also observed weak constitutive fragility of the RNU 1 and RNU 2 loci in cells belonging to xeroderma pigmentosum complementation groups B and D ( XPB and XPD ) which are partially defective in the ERCC 2 ( XPD ) and ERCC 3 ( XPB ) helicase activities shared between the repairosome and the RNA polymerase H basal transcription factor TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The RTT 4 gene is allelic with SSL 2 ( RAD 25 ) , which encodes a DNA helicase present in basal transcription ( TFIIH ) and nucleotide excision repair ( NER ) complexes . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The sun sensitive form of the severe neurodevelopmental , brittle hair disorder trichothiodystrophy ( TTD ) is caused by point mutations in the essential XPB and XPD helicase subunits of the dual functional DNA repair / basal transcription factor TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Some NER genes , including the DNA helicases XPB and XPD , have been shown to function in transcription as well as repair , by virtue of being an integral part of the transcription initiation factor TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
TTD is a rare human genetic disease caused by mutations in XPB and XPD , two subunits of the transcription / repair factor TFIIH , and whose outstanding clinical characteristic is a lack of most human UHS proteins resulting in sulfur deficient brittle hair . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Reconstitution of the transcription factor TFIIH : assignment of functions for the three enzymatic subunits , XPB , XPD , and cdk 7 . ^^^ Having succeeded in reconstituting a functionally active TFIIH from baculovirus recombinant polypeptides , we were able to analyze the role of XPB , XPD , and cdk 7 subunits in the transcription reaction . ^^^ Designing mutated recombinant subunits , we show that the XPB helicase is absolutely required for transcription to open the promoter around the start site whereas the XPD helicase , which is dispensable , stimulates transcription and allows the CAK complex to be anchored to TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Mutations in XPB and XPD helicases found in xeroderma pigmentosum patients impair the transcription function of TFIIH . ^^^ As part of TFIIH , XPB and XPD helicases have been shown to play a role in nucleotide excision repair ( NER ) . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Our previous work has shown that HBx protein inhibits p 53 sequence specific transcriptional activation , p 53 mediated apoptosis and p 53 binding to the TFIIH transcription nucleotide excision repair ( NER ) factors , including XPB and XPD . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Initial recognition of the distortion is the most likely function for XPC hHR23B and perhaps XPA and RPA , whereas TFIIH is well suited to locate the damaged DNA strand by locating altered DNA chemistry that blocks translocation of the XPB and XPD components . . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The human XPB DNA helicase is a subunit of the DNA repair / basal transcription factor TFIIH , involved in early steps of the nucleotide excision repair pathway . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
GST XPB ( 203 782 ) was localized predominantly in the cytoplasm and bound to BCR but not to p 62 , one of the other components in TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The Rad 25 protein in yeast is a DNA helicase and a subunit of the general transcription factor TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
A role for the TFIIH XPB DNA helicase in promoter escape by RNA polymerase 2 . ^^^ TFIIH possesses three known ATP dependent activities : a 3 ' > 5 ' DNA helicase catalyzed by its XPB subunit , a 5 ' > 3 ' DNA helicase catalyzed by its XPD subunit , and a carboxyl terminal domain ( CTD ) kinase activity catalyzed by its CDK 7 subunit . ^^^ Our findings argue that the TFIIH XPB DNA helicase is primarily responsible for preventing premature arrest of early elongation intermediates during exit of polymerase from the promoter . . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We previously reported that p 53 mediated apoptosis is attenuated in primary human fibroblasts from individuals with Xeroderma Pigmentosum ( XP ) that harbor mutations in the TFIIH DNA helicases XPD or XPB . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
To provide an explanation of some clinical features observed within rare xeroderma pigmentosum ( XP ) patients and to further define the role of XPB , XPD , and cdk 7 , the three enzymatic subunits of TFIIH , in the transcription reaction , we have examined two defined enzymatic steps : phosphodiester bond formation and promoter escape . ^^^ We demonstrate that XPB patient derived mutants in TFIIH suffer from defects in initiation . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Mutations in the DNA dependent ATPase / helicase subunits of TFIIH , XPB and XPD , are associated with three inherited syndromes as follows : xeroderma pigmentosum with or without Cockayne syndrome and trichothiodystrophy . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The most complex and difficult to analyze factor is TFIIH , which has a 6 subunit core ( XPB , XPD , p 44 , p 34 , p 52 , p 62 ) and a 3 subunit kinase ( CAK ) . ^^^ TFIIH from cells with XPB or XPD mutations was defective in supporting repair , whereas TFIIH from spinal muscular atrophy cells with a deletion of one p 44 gene was active . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
In contrast , mutations in two members of the TFIIH complex , the XPB and XPD genes are generally very severe with both skin and CNS disorders . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Here we show that certain mutant alleles of the nucleotide excision repair ( NER ) / TFIIH helicase genes RAD 3 and SSL 2 ( RAD 25 ) confer synthetic lethality and destabilize the Saccharomyces cerevisiae genome by increasing both short sequence recombination and Ty 1 retrotransposition . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
This interaction is thought to be mediated through the specific affinity of XPC for the TFIIH subunits XPB and / or p 62 , which are essential for both basal transcription and nucleotide excision repair . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The median portion of MAT 1 , which contains a coiled coil motif , allows the binding of CAK to the TFIIH core through interactions with both XPD and XPB helicases . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
This reaction requires the damage binding factors Rad 14 , RPA , and the Rad 4 Rad23 complex , the transcription factor TFIIH which contains the two DNA helicases Rad 3 and Rad 25 , essential for creating a bubble structure , and the two endonucleases , the Rad 1 Rad10 complex and Rad 2 , which incise the damaged DNA strand on the 5 ' and 3 ' side of the lesion , respectively . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The p89 / xeroderma pigmentosum complementation group B ( XPB ) ATPase helicase of transcription factor IIH ( TFIIH ) is essential for promoter melting prior to transcription initiation by RNA polymerase 2 ( RNAPII ) . ^^^ A mutation in p89 / XPB found in a xeroderma pigmentosum patient impairs the ability of TFIIH to associate correctly with the complex and thereby melt promoter DNA . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The two helicases , XPB and XPD , are components of the basal transcription factor TFIIH , which has a dual role in NER and initiation of transcription . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The repair deficient form of trichothiodystrophy ( TTD ) most often results from mutations in the genes XPB or XPD , encoding helicases of the transcription / repair factor TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Cloning of a human homolog of the yeast nucleotide excision repair gene MMS 19 and interaction with transcription repair factor TFIIH via the XPB and XPD helicases . ^^^ Co immunoprecipitation experiments revealed that hMMS 19 directly interacts with the XPB and XPD subunits of NER transcription factor TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
In both processes , TFIIH is implicated with local DNA unwinding , which is attributed to its helicase subunits XPB and XPD . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We report here the different ways in which four subunits of the basal transcription / repair factor TFIIH ( XPB , XPD , p 62 and p 44 ) and the damage recognition XPC repair protein can enter the nucleus . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Transcriptional regulation of the TFIIH transcription repair components XPB and XPD by the hepatitis B virus 10 protein in liver cells and transgenic liver tissue . ^^^ Herein we report that HBx represses two components of the transcription repair factor TFIIH , XPB ( p 89 ) , and XPD ( p 80 ) , both in p 53 proficient and p 53 deficient liver cells . ^^^ Expression of HBx in liver cells results in down regulation of endogenous XPB and XPD mRNAs and proteins ; this inhibition is not observed with other TFIIH subunits , XPA or PCNA . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
TFIIH is a multifunctional RNA polymerase 2 general initiation factor that includes two DNA helicases encoded by the Xeroderma pigmentosum complementation group B ( XPB ) and D ( XPD ) genes and a cyclin dependent protein kinase encoded by the CDK 7 gene . ^^^ Previous studies have shown that the TFIIH XPB DNA helicase plays critical roles not only in transcription initiation , where it catalyzes ATP dependent formation of the open complex , but also in efficient promoter escape , where it suppresses arrest of very early RNA polymerase 2 elongation intermediates . ^^^ In this report , we present evidence that ATP dependent TFIIH action in transcription initiation and promoter escape requires distinct regions of the DNA template ; these regions are well separated from the promoter region unwound by the XPB DNA helicase and extend , respectively , approximately 23 39 and approximately 39 50 bp downstream from the transcriptional start site . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
In UV sensitive TTD , the TFIIH transcription factor containing XPB and XPD helicase activities necessary for both transcription initiation and DNA repair is damaged . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
As biochemists , we have characterized each component of TFIIH , three of which are XPB and XPD helicases and cdk 7 , a cyclin dependent kinase . ^^^ We now know how the XPB helicase opens the promoter region for RNA synthesis and that one of the roles of XPD helicase is to anchor the cdk 7 kinase to the core TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
By using recombinant TFIIH subcomplexes , our results suggest that the XPB subunit of TFIIH is responsible for this inhibition of CDK 9 phosphorylation . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The regulatory role for the ERCC 3 helicase of general transcription factor TFIIH during promoter escape in transcriptional activation . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Mediates XPB function within the transcription / repair factor TFIIH . ^^^ Moreover , we demonstrate that intact p 52 is needed to anchor the XPB helicase within TFIIH , providing an explanation for the transcription and NER defects observed with the mutant p 52 . ^^^ Taken together , our results show that the p52 / Tfb2 subunit of TFIIH regulates the function of XPB through pair wise interactions as described previously for p 44 and XPD . . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Mutations in XPB and XPD TFIIH helicases have been related with three hereditary human disorders : xeroderma pigmentosum , Cockayne syndrome , and trichothiodystrophy . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We have recently completed screening of the National Cancer Institute human tumor cell line panel and demonstrated that among four nucleotide excision repair proteins ( XPA , XPB , XPD , and ERCC 1 ) , only the TFIIH subunit XPD endogenous protein levels correlate with alkylating agent drug resistance . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
In this report , we demonstrate an association of a C terminal domain of human RAD 52 ( amino acids 302 418 ) with the XPB and XPD subunits of transcription factor TFIIH and RNA polymerase 2 ( RNAPII ) . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Trichothiodystrophy ( TTD ) is a rare hereditary multisystem disorder associated with defects in nucleotide excision repair ( NER ) as a consequence of mutations in XPD , XPB or TTDA , three genes that are all related to TFIIH , the multiprotein complex involved in NER and transcription . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We also show that mutations in CSB , as well as in XPB and XPD genes , all of which confer CS , disturb the RNA pol I / TFIIH interaction within the CSB IP / 150 . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Alleviation of PC 4 mediated transcriptional repression by the ERCC 3 helicase activity of general transcription factor TFIIH . ^^^ Using recombinant TFIIH , we have analyzed the role of TFIIH for alleviating PC 4 mediated transcriptional repression and determined that the excision repair cross complementing ( ERCC 3 ) helicase activity of TFIIH is the enzymatic activity that alleviates PC 4 mediated repression via beta gamma bond hydrolysis of ATP . ^^^ Thus , TFIIH appears to protect promoters from PC 4 mediated repression by relieving the topological constraint imposed by PC 4 through the ERCC 3 helicase activity rather than by reducing the repressive activity of PC 4 via its phosphorylation . . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The TFIIH holoenzyme , including XPB and XPD helicases , is absolutely required for transcription coupled ( TCR ) as well as global genome ( GGR ) NER pathways . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
XPB and XPD proteins are components of transcription factor TFIIH , which is involved in both basal and activated transcription . ^^^ XPB is part of the core of TFIIH and has a central role in transcription , whereas XPD connects the core to the CAK subcomplex , and can tolerate many different mutations . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Using yeast two hybrid system , recombinant technology , and confocal microscopy , we further demonstrated that the nonstructural viral NSs protein interacts with the p 44 component of TFIIH to form nuclear filamentous structures that also contain XPB subunit of TFIIH . ^^^ By competing with XPD , the natural partner of p 44 within TFIIH , and sequestering p 44 and XPB subunits , NSs prevents the assembly of TFIIH subunits , thus destabilizing the normal host cell life . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
DNA repair deficient trichothiodystrophy ( TTD ) results from mutations in the XPD and XPB subunits of the DNA repair and transcription factor TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
RNA polymerase 2 140wimp mutant and mutations in the TFIIH subunit XPB differentially affect homeotic gene expression in Drosophila . ^^^ Mutations in the XPB and XPD helicases of the DNA repair / transcription factor TFIIH are involved in several human genetic disorders . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The N terminal two mutants bound stronger to the small subunit of TFIIF than the wild type and the C terminal two mutants were impaired in their binding abilities to the XPB subunits of TFIIH . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Phosphorylation of XPB helicase regulates TFIIH nucleotide excision repair activity . ^^^ The xeroderma pigmentosum group B ( XPB ) helicase subunit of TFIIH functions in NER and transcription . ^^^ Finally , the phosphorylation of XPB S 751 does not impair the TFIIH unwinding of the DNA around the lesion , but rather prevents the 5 ' incision triggered by the ERCC 1 XPF endonuclease . ^^^ These data support an additional role for XPB in promoting the incision of the damaged fragment and reveal a point of NER regulation on TFIIH without interference in its transcription activity . . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We have compared cells expressing only a mutated p 89 ( xeroderma pigmentosum complementation group B [ XPB ] ) , the largest TFIIH subunit , with the same cells functionally complemented with the wild type protein ( XPB / wt p 89 ) . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Ssl 2 , the yeast homologue of XPB , is an ATP dependent DNA helicase that is a component of the TFIIH multiprotein complex and has dual functions in transcription and DNA repair . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
XPB , a subunit of TFIIH , contains an ATP dependent helicase activity that is used in both of these processes . ^^^ Mutants were constructed that are defective in stimulating the XPB helicase but still allow intact TFIIE to bind and recruit XPB and TFIIH to form the pre initiation complex . ^^^ The data suggest that the beta subunit of TFIIE is an ATPase and helicase co factor that can assist the XPB subunit of TFIIH during transcription initiation and the transition to early elongation , enhancing the potential diversity of regulatory targets . . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
TFIIH XPB mutants suggest a unified bacterial like mechanism for promoter opening but not escape . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
XPB is a subunit of a multifunctional RNA polymerase 2 general initiation factor TFIIH and codes for 3 ' > 5 ' DNA helicase essential for both nucleotide excision repair ( NER ) and transcription . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The severe xeroderma pigmentosum / Cockayne syndrome ( XP / CS ) syndrome is caused by mutations in the XPB , XPD and XPG genes that encode the helicase subunits of TFIIH and the 3 ' endonuclease of nucleotide excision repair ( NER ) . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Mutation of CSB , CSA , or the TFIIH helicases XPB and XPD can also cause defective TCR and CS . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
TFIIH DNA helicases , XPB and XPD , are also components in this apoptotic pathway . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
These studies indicate that the hsp 70 promoter is already occupied by TATA binding protein ( TBP ) and several TBP associated factors ( TAFs ) , TFIIB , TFIIF ( RAP 30 ) , TFIIH ( XPB ) , TBP free / TAF containg complex ( GCN 5 and TRRAP ) , and the Mediator complex subunit 13 before heat shock . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Here , we reveal that p8 / TTD A , the tenth subunit of TFIIH , has a critical role in DNA repair where it triggers DNA opening by stimulating XPB ATPase activity together with the damage recognition factor XPC hHR23B . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
We demonstrate that the human TFIIH complex proteins XPB ( ERCC 3 ) and XPD ( ERCC 2 ) play a principal role in the degradation of retroviral cDNA . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
Plant homologues of the human TFIIH subunits XPB and XPD that function in NER have been isolated but none has been shown to operate in transcription . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
In XPB cells carrying mutant TFIIH , loop formation failed and the serum response was abnormal ; RNAi depletion of FIR similarly disabled c myc regulation . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
The human xeroderma pigmentosum group B ( XPB ) helicase is essential for transcription , nucleotide excision repair , and TFIIH functional assembly . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
DNA downstream of the transcription bubble maps to a path between the two helicase subdomains of the TFIIH subunit Rad 25 ( also called XPB ) . ^^^
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92759 and P19447	Pubmed	SVM Score :0.0
NA