| Interacting proteins: Q92729 and P09471 |
Pubmed |
SVM Score :0.0 |
| Pcp 2 ( Purkinje cell protein 2 ) was identified as a partner for Galphao in this system . ^^^ Pcp 2 is expressed in cerebellar Purkinje cells and retinal bipolar neurons , two locations where Galphao is also expressed . ^^^ Galphao and Pcp 2 binding was confirmed in vitro using glutathione S transferase Pcp 2 fusion proteins and in vitro translated [ 35S ] methionine labeled Galphao . ^^^ In addition , when Galphao and Pcp 2 were cotransfected into COS cells , Galphao was detected in immunoprecipitates of Pcp 2 . ^^^ To determine whether Pcp 2 could modulate Galphao function , kinetic constants kcat and koff of bovine brain Galphao were determined in the presence and absence of Pcp 2 . ^^^ |
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| Interacting proteins: Q92729 and P09471 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that the first GoLoco motif ( GL 1 ) of PCP 2 is equipotent as a guanine nucleotide dissociation inhibitor ( GDI ) towards Galphai 1 and Galphai 2 , while it has sevenfold lower GDI activity for Galphai 3 and greater than 20 fold lower GDI activity against Galphao . ^^^ In contrast we found PCP 2 GL 2 to be essentially equipotent as a GDI for all Galphai subunits , but it had negligible activity toward Galphao . ^^^ Using co immunoprecipitation from COS 7 cells , we found that PCP 2 was only able to interact with Galphai 1 but not Galphao nor Galpha subunits from other families ( Galphas , Galphaq , or Galpha 12 ) . ^^^ |
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| Interacting proteins: Q92729 and P09471 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q92729 and P09471 |
Pubmed |
SVM Score :0.0 |
| NA |
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