| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.607154 |
| These findings demonstrate that Btk cooperates with SLP 65 as a tumor suppressor in pre B cells . 0.607154^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :1.4849603 |
| Since BLNK has been shown separately to interact with both Btk and PLC gamma 2 , our finding of normal Btk but impaired PLC gamma 2 activation in BCR stimulated BLNK ( ) / B cells strongly suggests that BLNK orchestrates the formation of a Btk PLC gamma 2 signaling axis that regulates NF kappaB activation . 1.4849603^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| In this report , we identify that one of the major Btk SH 2 domain binding proteins in B cells is BLNK ( B cell linker protein ) and present evidences that the interaction of BLNK and the SH 2 domain of Btk contributes to the complete tyrosine phosphorylation of PLCgamma . . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Until recently , the molecular mechanism underlying the double requirement for Syk and Btk in PLC gamma 2 activation remained unclear , but new data suggest that an adapter molecule , B cell linker protein ( alternatively named SLP 65 or BASH ) , phosphorylated by Syk , provides docking sites for Btk SH 2 domain as well as PLC gamma 2 SH2 domains , thus bringing Btk into close proximity with PLC gamma 2 . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Recently , the B cell linker protein ( BLNK ) was found to interact with the SH 2 domain of Btk , and this association is required for the activation of phospholipase C gamma . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Cbl b interacted with PLC gamma 2 and helped the association of PLC gamma 2 with Bruton ' s tyrosine kinase ( Btk ) , as well as B cell linker protein ( BLNK ) . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Current models of lymphocyte activation suggest that formation of a signaling complex , or `` signalosome ' ' , composed of Syk , Bruton ' s tyrosine kinase ( Btk ) , phospholipase gamma 2 and the adaptor protein B cell linker protein ( BLNK ) is critical for transmission of signals from the BCR . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Recent results obtained in mice deficient either in an adaptor molecule , BLNK ( alternatively named SLP 65 or BASH ) , or in phosphatidylinositol 3 kinase have revealed similar though not identical phenotypes to those of Btk ( / ) mice , suggesting a functional link between BLNK , Btk and phosphatidylinositol 3 kinase . . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| The absence of SLP 65 and Btk blocks B cell development at the preB cell receptor positive stage . ^^^ To investigate the consequences of mutations in both SLP 65 and Btk , we generated SLP65 / Btk double mutant mice by crossing the single mutant mice . ^^^ Together , the data demonstrate the synergistic role of SLP 65 and Btk in B cell development and describe a situation where large numbers of preB cell receptor positive cells accumulate in the bone marrow and spleen . . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| During B cell development in the mouse , Bruton tyrosine kinase ( Btk ) and the adaptor protein SLP 65 ( Src homology 2 [ SH 2 ] domain containing leukocyte protein of 65 kDa ) limit the expansion and promote the differentiation of pre B cells . ^^^ Btk is thought to mainly function by phosphorylating phospholipase Cgamma 2 , which is brought into close proximity of Btk by SLP 65 . ^^^ However , this model was recently challenged by the identification of a role for Btk as a tumor suppressor in the absence of SLP 65 and by the finding that Btk function is partially independent of its kinase activity . ^^^ To investigate if enzymatic activity is critical for the tumor suppressor function of Btk , we crossed transgenic mice expressing the kinase inactive K430R Btk mutant onto a Btk / SLP 65 double deficient background . ^^^ We found that K430R Btk expression rescued the severe developmental arrest at the pre B cell stage in Btk / SLP 65 double deficient mice . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Specific phosphoacceptor sites in SLP 65 serve as docking sites for Ca ( 2+ ) mobilizing enzymes Btk and PLC gamma 2 . ^^^ Thus , elevation of Ca ( 2+ ) is regulated by at least two signaling modules , the B cell specific Ca ( 2+ ) initiation complex comprising SLP 65 , Btk , and PLC gamma 2 and the more ubiquitously expressed NTAL / Grb2 complex , which acts as an amplifier by switching off inhibitory elements . . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Involvement of SLP 65 and Btk in tumor suppression and malignant transformation of pre B cells . ^^^ In the mouse , the downstream signaling molecules SLP 65 and Btk cooperate to limit proliferation and induce differentiation of pre B cells , thereby acting as tumor suppressors to prevent pre B cell leukemia . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Bruton ' s tyrosine kinase ( Btk ) and the adapter protein SLP 65 ( Src homology 2 domain containing leukocyte specific phosphoprotein of 65 kDa ) transmit precursor BCR ( pre BCR ) signals that are essential for efficient developmental progression of large cycling into small resting pre B cells . ^^^ We show that Btk and SLP 65 deficient pre B cells have a specific defect in Ig lambda L chain germline transcription . ^^^ In Btk / SLP 65 double deficient pre B cells , both kappa and lambda germline transcripts are severely reduced . ^^^ Although these observations point to an important role for Btk and SLP 65 in the initiation of L chain gene rearrangement , the possibility remained that these signaling molecules are only required for termination of pre B cell proliferation or for pre B cell survival , whereby differentiation and L chain rearrangement is subsequently initiated in a Btk / SLP 65 independent fashion . ^^^ Because transgenic expression of the antiapoptotic protein Bcl 2 did not rescue the developmental arrest of Btk / SLP 65 double deficient pre B cells , we conclude that defective L chain opening in Btk / SLP 65 deficient small resting pre B cells is not due to their reduced survival . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| BASH binds to various signaling intermediates , such as Btk , PLCgamma 2 , Vav , and Grb 2 , through its well defined motifs . ^^^ BNAS 2 was co precipitated with BASH as well as Btk and ERK 2 from a lysate of mouse B cell line . ^^^ Thus BNAS 2 may serve as a scaffold for signaling proteins such as BASH , Btk , and ERK at the ER and nuclear membrane and may facilitate ERK activation by signaling from cell surface receptors . . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| BCR induced inhibition of SDF 1alpha chemotaxis was dependent on Syk , BLNK , Btk , and phospholipase C ( Plc ) gamma 2 but independent of Ca2+ mobilization , suggesting that the target of BCR stimulation was a protein kinase C ( PKC ) dependent substrate . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| BLNK : connecting Syk and Btk to calcium signals . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Overall , the phenotype of BLNK ( / ) mice bears a striking resemblance to that of xid mice which is the murine model of human XLA that has a mutation in Bruton ' s tyrosine kinase . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| These events promoted Lyn activation and Igbeta phosphorylation and led to the generation of a raft associated signaling module composed of tyrosine phosphorylated Lyn , Syk , BLNK , PI3K , Btk , VAV , and PLCgamma 2 . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Expression and tyrosine phosphorylation of Btk , BLNK , Vav , or phosphatidylinositol 3 kinase were equivalent . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Defects in Btk , lambda 5 and BLNK result in a more severe phenotype in the human compared to the mouse . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| BLNK mediates Syk dependent Btk activation . ^^^ We have demonstrated that BLNK mediates Syk dependent Btk activation . ^^^ In a reconstitution cell system , coexpression of BLNK allows Syk to phosphorylate Btk on its tyrosine 551 , leading to the enhancement of Btk activity . ^^^ This phosphorylation depends on the interaction of Btk and BLNK by means of the Btk Src homology 2 domain . ^^^ The existence of such an activation mechanism is supported by the observation that the BCR induced Btk phosphorylation and activation are significantly reduced in BLNK deficient B cells as well as in Syk deficient B cells . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Btk and BLNK in B cell development . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Of the two kinases that previously have been proposed to phosphorylate PLCgamma 2 , Btk , and Syk , purified Btk had much greater ability to phosphorylate recombinant PLCgamma 2 in vitro , whereas Syk efficiently phosphorylated adapter protein BLNK . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Collectively , these data provide evidence for the concept that the B cell signalosome ( p85alpha , Btk , BLNK and PLCgamma ) is involved in regulating cyclin D 2 expression in response to BCR engagement . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| The stable expression of Lyn PLCgamma 2 was not accompanied by an increase in substrate hydrolysis in resting cells , which followed stimulation and specifically required the presence and / or activation of Syk , Btk , phosphoinositide 3 kinase but not BLNK , as established using deficient cell lines or specific inhibitors . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Significantly , 2 APB induced InsP ( 3 ) R and TRPC 3 activation was prevented in DT 40 knockout cells devoid of the BCR and PLC gamma 2 coupled adaptor / kinases , Syk , Lyn , Btk or BLNK . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Here we outline known discrepancies in both innate and adaptive immunity , including : balance of leukocyte subsets , defensins , Toll receptors , inducible NO synthase , the NK inhibitory receptor families Ly 49 and KIR , FcR , Ig subsets , the B cell ( BLNK , Btk , and lambda 5 ) and T cell ( ZAP 70 and common gamma chain ) signaling pathway components , Thy 1 , gammadelta T cells , cytokines and cytokine receptors , Th1 / Th2 differentiation , costimulatory molecule expression and function , Ag presenting function of endothelial cells , and chemokine and chemokine receptor expression . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Tec induced NFAT activation requires PLC gamma , but not the adapters LAT , SLP 76 , and BLNK , which are required for Btk and Itk to couple to PLC gamma . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| Here , we show that BCR signaling in Y 112 LMP2A expressing cells is attenuated with a reduction in both the degree and duration of phosphorylation of key components of the BCR signaling cascade including Syk , BLNK , PI3K , and Btk . ^^^ |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WV28 and Q06187 |
Pubmed |
SVM Score :0.0 |
| NA |
|