| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| We describe here the identification of a novel B cell linker protein , termed BLNK , that interfaces the B cell receptor associated Syk tyrosine kinase with PLCgamma , the Vav guanine nucleotide exchange factor , and the Grb 2 and Nck adapter proteins . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| BLNK ( B cell LiNKer protein ) , a substrate for Syk , is now shown to be essential in activating phospholipase C ( PLC ) gamma 2 and JNK . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| In B cells , B cell linker protein ( BLNK ) links the B cell receptor ( BCR ) activated Syk kinase to the phosphoinositide and mitogen activated kinase pathways . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Until recently , the molecular mechanism underlying the double requirement for Syk and Btk in PLC gamma 2 activation remained unclear , but new data suggest that an adapter molecule , B cell linker protein ( alternatively named SLP 65 or BASH ) , phosphorylated by Syk , provides docking sites for Btk SH 2 domain as well as PLC gamma 2 SH2 domains , thus bringing Btk into close proximity with PLC gamma 2 . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Closely related molecules with overlapping functions , such as the Syk kinases , SYK , and ZAP 70 , and adaptor molecules , SH 2 domain containing leukocyte protein of 76 kDa and B cell linker protein , are expressed in activated NK cells and are all recruited to the center of the cytolytic synapse . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Current models of lymphocyte activation suggest that formation of a signaling complex , or `` signalosome ' ' , composed of Syk , Bruton ' s tyrosine kinase ( Btk ) , phospholipase gamma 2 and the adaptor protein B cell linker protein ( BLNK ) is critical for transmission of signals from the BCR . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Mutant B cells , present in normal numbers , exhibited unimpaired BCR induced spleen tyrosine kinase ( Syk ) phosphorylation but reduced B cell linker protein ( BLNK ) phosphorylation , calcium flux , and nuclear factor kappaB ( NFkappaB ) , c jun N terminal kinase ( JNK ) , and extracellular signal regulated kinase ( ERK ) activation . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Induction of the antigen receptor expression on B lymphocytes results in rapid competence for signaling of SLP 65 and Syk . ^^^ The binding of antigen to the B cell antigen receptor ( BCR ) results in the activation of protein tyrosine kinases ( PTKs ) such as Lyn and Syk , and the phosphorylation of several substrate proteins including HS 1 and SLP 65 . ^^^ By monitoring the activation of signaling elements in pervanadate stimulated B cells expressing different levels of the BCR , we show here that phosphorylation of SLP 65 and Syk , but not of Lyn , is strictly dependent on the expression of the BCR on the cell surface . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| The developmental progression from pro B to pre B cells is controlled by pre B cell receptor ( pre BCR ) signaling which depends on BLNK ( SLP 65 ) for coupling the Syk kinase to its downstream effector pathways . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Investigating possible interference of BCR ABL 1 with pre B cell receptor signaling , we found that neither SYK nor SLP 65 can be phosphorylated in response to pre B cell receptor engagement . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| BASH becomes tyrosine phosphorylated with the B cell Ag receptor ( BCR ) cross link or by coexpression with Syk and Lyn and associates with signaling molecules including Syk and a putative chicken Shc homologue . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| The B cell restricted adaptor protein BASH ( also termed BLNK / SLP 65 ) is rapidly phosphorylated by the tyrosine kinase Syk after BCR ligation and binds to various signaling proteins . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| BCR ligation induced rapid tyrosine phosphorylation of HPK 1 mainly by Syk and Lyn , resulting in its association with BASH and catalytic activation . ^^^ BCR mediated activation of HPK 1 was impaired in Syk or BASH deficient B cells . ^^^ The functional SH 2 domain of BASH and Tyr 379 within HPK 1 which we identified as a Syk phosphorylation site were both necessary for interaction of both proteins and efficient HPK 1 activation after BCR stimulation . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| BCR induced inhibition of SDF 1alpha chemotaxis was dependent on Syk , BLNK , Btk , and phospholipase C ( Plc ) gamma 2 but independent of Ca2+ mobilization , suggesting that the target of BCR stimulation was a protein kinase C ( PKC ) dependent substrate . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Further analysis shows that Igalpha is phosphorylated and syk is recruited and becomes phosphorylated but that BLNK and PLCgamma phosphorylation are defective in mutant cells . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| BLNK : connecting Syk and Btk to calcium signals . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| The adaptor protein BLNK / SLP 65 plays a pivotal role in BCR signal transduction by coupling Syk activation to downstream elements such as Grb 2 , phospholipase C gamma , Vav and Nck . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| In vitro kinase assays demonstrated that hyperphosphorylation of BLNK in SHP 1 C / S expressing cells was not due to enhanced activity of Lyn or Syk . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| These events promoted Lyn activation and Igbeta phosphorylation and led to the generation of a raft associated signaling module composed of tyrosine phosphorylated Lyn , Syk , BLNK , PI3K , Btk , VAV , and PLCgamma 2 . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| This differs from mice lacking the tyrosine kinase Syk that is upstream of BLNK in BCR signaling and contrasts with mice lacking SLP 76 that is the equivalent adaptor molecule in TCR signal transduction . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| BLNK mediates Syk dependent Btk activation . ^^^ We have demonstrated that BLNK mediates Syk dependent Btk activation . ^^^ In a reconstitution cell system , coexpression of BLNK allows Syk to phosphorylate Btk on its tyrosine 551 , leading to the enhancement of Btk activity . ^^^ The existence of such an activation mechanism is supported by the observation that the BCR induced Btk phosphorylation and activation are significantly reduced in BLNK deficient B cells as well as in Syk deficient B cells . ^^^ Although previous observations have identified the function of BLNK as the linker that integrates the action of Btk and Syk into downstream effectors such as phospholipase Cgamma 2 , our present study indicates another function of BLNK that connects the activity of Syk to that of Btk . . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Hematopoietic cell specific adapter proteins , SLP 76 and BLNK , effectively activate NF AT as well as NF kappaB by Syk and Tec PTKs in non lymphoid cell lines . ^^^ We show that SLP 76 and BLNK , but not LAT , effectively co operated with Syk and Tec family PTKs to activate NF AT and NF kappaB . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Of the two kinases that previously have been proposed to phosphorylate PLCgamma 2 , Btk , and Syk , purified Btk had much greater ability to phosphorylate recombinant PLCgamma 2 in vitro , whereas Syk efficiently phosphorylated adapter protein BLNK . ^^^ Using purified proteins to analyze the formation of complexes , we suggest that function of Syk is to phosphorylate BLNK , providing binding sites for PLCgamma 2 . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| H2O2 treatment of B cells induced a rapid tyrosine phosphorylation of BLNK in a H2O2 dose dependent manner , which was inhibited in Syk deficient DT 40 cells . ^^^ Calcium mobilization in BLNK deficient as well as Syk deficient and phospholipase C ( PLC ) gamma 2 deficient cells after H2O2 treatment was completely abolished . ^^^ Furthermore , c Jun N terminal kinase activation following high doses of H2O2 stimulation , but not low doses of H2O2 stimulation , was abrogated in BLNK deficient as well as Syk deficient cells . ^^^ These findings have led to the suggestion that BLNK is required for coupling Syk to PLC gamma 2 , thereby accelerating cell apoptosis in B cells exposed to low doses of H2O2 . . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| The coupling of Syk to several distal substrates requires linker protein BLNK . ^^^ These data demonstrate that the non ITAM tyrosines of Ig alpha couple Syk activation to BLNK dependent pathways . . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| The stable expression of Lyn PLCgamma 2 was not accompanied by an increase in substrate hydrolysis in resting cells , which followed stimulation and specifically required the presence and / or activation of Syk , Btk , phosphoinositide 3 kinase but not BLNK , as established using deficient cell lines or specific inhibitors . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| The Src family kinase Syk , the B cell adaptor protein BLNK , and phospholipase C ( PLC ) gamma 2 were consistently absent from Reed Sternberg cells , whereas 2 other Src kinases ( Lyn and Fyn ) were heterogeneously expressed in a proportion of cases ( 12 % and 42 % , respectively ) . ^^^ In contrast , the tumor cells in all cases of lymphocyte predominance Hodgkin disease were positive for Fyn , Syk , BLNK , and PLC gamma 2 , and Lyn immunostaining was seen in a minority of biopsies . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Significantly , 2 APB induced InsP ( 3 ) R and TRPC 3 activation was prevented in DT 40 knockout cells devoid of the BCR and PLC gamma 2 coupled adaptor / kinases , Syk , Lyn , Btk or BLNK . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| We obtained selective labelling of white cells for eight such molecules ( the ' linker ' molecules SLP 76 and BLNK , the Src family kinases Lyn , Fyn , Syk and Hck , and the phospholipases PLC gamma 1 and PLC gamma 2 ) . ^^^ Antibodies to SLP 76 and PLC gamma 1 selectively labelled T cells , and antibodies to BLNK , Lyn , Fyn , Syk and PLC gamma 2 labelled B cells ( although Fyn immunostaining was restricted to mantle zone B cells ) . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| When pre BCR was cross linked with anti micro heavy chain antibodies , significant phosphorylation of intracellular molecules , including Syk , Shc , ERK MAP kinase , and AKT , and an activation of Ras were observed without regard to deficiency of BLNK expression , suggesting that BLNK is not required for pre BCR mediated activation of MAP kinase and phosphatidyl inositol 3 ( PI 3 ) kinase signalling . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| We report that these signaling molecules are frequently present , and in particular that Syk , BLNK and PLC gamma 2 ( absent from Reed Sternberg cells ) are present in the majority of mediastinal B cell lymphomas . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Following IgM ligation , ZAP 70+ CLL cells had significantly higher levels of phosphorylated p 72 ( Syk ) , BLNK , and phospholipase Cgamma ( PLCgamma ) and had greater [ Ca2+ ] 1 flux than did ZAP 70 negative CLL cases , including unusual ZAP 70 negative cases with unmutated IgV ( H ) . ^^^ IgM ligation of ZAP 70 negative CLL B cells infected with an adenovirus vector encoding ZAP 70 induced significantly greater levels of phosphorylated p 72 ( Syk ) , BLNK , and PLCgamma and had greater [ Ca2+ ] 1 flux than did similarly stimulated , noninfected CLL cells or CLL cells infected with a control adenovirus vector . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Similarly , there was a marked difference between VH unmutated cases using the V 1 69 gene and VH UM ( up : FOS ; down : BLNK , SYK , CDK 4 , TP 53 ) . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| Here , we show that BCR signaling in Y 112 LMP2A expressing cells is attenuated with a reduction in both the degree and duration of phosphorylation of key components of the BCR signaling cascade including Syk , BLNK , PI3K , and Btk . ^^^ |
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| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WV28 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
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