| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| LXR controls the transcription of crucial genes in cholesterol efflux from macrophages and its transport to the liver , such as ABCA 1 ( ATP binding cassette A 1 ) , CYP27A1 ( sterol 27 hydroxylase ) , CLA 1 ( scavenger receptor type B 1 ) and apolipoprotein E . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| In addition , in situ hybridization experiments revealed that SR BI mRNA was expressed in the thickened intima of atheromatous aorta of apolipoprotein E knockout mice . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Marten Hofker ( Leiden ) and Philippa Talmud ( London ) chaired Session 2 on ' Regulation of gene expression ' , which reported cellular regulations by nuclear receptors ( PPARs ) , or the regulation of lipid trafficking by membrane receptors ( SR BI , Megalin , Apo E receptor , scavenger receptors ) or by intracellular ( IFN gamma signalling pathways ) or extracellular proteins ( lipases ) . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Ligand blottings on liver membranes showed that purified HDL 1 , containing about 70 % apolipoprotein E and 10 % apolipoprotein AI , bind to the LDL receptor ( 130 kDa ) and not to HB 2 ( 100 kDa ) or SR BI ( 82 kDa ) , candidate HDL receptors . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| To further evaluate the role of SR BI in lipoprotein metabolism , compound apolipoprotein E knock out ( apoE 0 ) / SR BIatt mice were bred . ^^^ Hepatic SR BI protein was increased ( 2 . 3 fold ) in apoE 0 mice compared with wild type ( wt ) and was reduced significantly in apoE0 / SR BIatt mice . ^^^ The results suggest that apoE plays a major role in the selective clearance of HDL CE by the liver and adrenal gland , possibly by facilitating the presentation of HDL to SR BI at the cell surface . . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| The reduction in cholesterol efflux was independent of apoE synthesis or SR BI expression and was associated with a redistribution of intracellular cholesterol with an increase in cholesteryl ester accumulation . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| In contrast , the mRNA levels of the potential regulatory proteins of the HDL level such as apoA 1 , apoE , LCAT , PLTP , SRB 1 and ABC 1 did not change with probucol . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| In transfected cells SR BI recognizes HDL , low density lipoprotein ( LDL ) and modified LDL , protein free lipid vesicles containing anionic phospholipids , and recombinant lipoproteins containing apolipoprotein ( apo ) A 1 , apoA 2 , apoE , or apoCIII . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Based on available data in animal models , some gene products are candidates for pharmacologic upregulation , infusion , or overexpression , including apolipoprotein ( apo ) A 1 , apoE , apoA 4 , lipoprotein lipase ( LPL ) , ATP binding cassette protein 1 ( ABC 1 ) , lecithin cholesterol acyltransferase ( LCAT ) , and scavenger receptor B 1 ( SR BI ) . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Erythrocytes from SR BI ( / ) / apolipoprotein E ( / ) mice and SR BI ( / ) mice that were fed cholesterol had markedly increased membrane cholesterol . ^^^ Autophagolysosomes from SR BI ( / ) / apolipoprotein E ( / ) erythrocytes were expelled when the erythrocytes were transfused into wild type animals or incubated in vitro with normolipidemic serum or the cholesterol sequestering agent methyl cyclodextrin . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Macrophages express a number of proteins involved in sterol efflux pathways , including apolipoprotein E ( apoE ) and scavenger receptor class B type 1 ( SR BI ) . ^^^ We show that in apoE expressing cells , a 4 fold increase in SR BI expression leads to reduction of sterol and phospholipid efflux . ^^^ SR BI mediated reduction in sterol efflux was only observed in cells that expressed endogenous apoE . ^^^ In J 774 cells that did not express apoE , a similar increase in SR BI level led to increased sterol efflux . ^^^ Increased SR BI expression also enhanced sterol efflux to exogenously added apoE . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Candidate genes were apolipoprotein A 4 ( apoA 4 ) , scavenger receptor BI ( SR BI ) , cholesterol ester transfer protein ( CETP ) , 3 hydroxy 3 methylglutaryl coenzyme A ( HMG CoA ) reductase , and apolipoprotein E ( apoE ) . ^^^ CONCLUSION : These findings suggest that all subjects who want to lower their cholesterol concentration , will benefit from plant stanol ester consumption , irrespective of their apoA 4 , SR BI , HMG CoA reductase , CETP , or apoE genotype . . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Our results suggest that SR BI are present in the parietal cortex as well as in the cerebellum of the control and AD brains , suggesting that the presence of SR BI may be involved in the uptake of oxidatively modified lipoproteins and beta amyloid ( Abeta ) protein complexed with apoE , suggesting implications in the progression of late onset AD and other neurodegenerative disorders characterized by the deposition of insoluble aggregates observed in the AD brain . . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Here we show that mice with homozygous null mutations in the genes for both the high density lipoprotein receptor SR BI and apoE ( SR BI / apoE double knockout [ dKO ] mice ) exhibit morphological and functional defects with similarities to those seen in human CHD . ^^^ Thus , SR BI / apoE dKO mice provide a new murine model for CHD and may help better define the role of lipoprotein metabolism and atherosclerosis in the pathogenesis of myocardial infarction and cardiac dysfunction . ^^^ Loss of SR BI expression leads to the early onset of occlusive atherosclerotic coronary artery disease , spontaneous myocardial infarctions , severe cardiac dysfunction , and premature death in apolipoprotein E deficient mice . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| POPC apoE particles generated using apoE 2 , apoE 3 , apoE 4 , or the carboxyl terminally truncated forms apoE 165 , apoE 202 , apoE 229 , and apoE 259 all bound tightly to wild type SR BI with similar affinities ( K ( d ) = 35 45 microg / ml ) . ^^^ The findings establish that apoE is a ligand for SR BI and that the receptor binding domain is located in the amino terminal 1 165 region of the protein . ^^^ SR BI apoE interactions may contribute to cholesterol homeostasis in tissues and cells expressing SR BI that are accessible to apoE containing lipoproteins . . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Polymorphisms of apolipoprotein E ( apoE ) and scavenger receptor class B type 1 ( SR BI ) were observed to have minor effects on the lipid and lipoprotein profile . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Probucol prevents early coronary heart disease and death in the high density lipoprotein receptor SR BI / apolipoprotein E double knockout mouse . ^^^ Mice with homozygous null mutations in the high density lipoprotein receptor SR BI ( scavenger receptor class B , type 1 ) and apolipoprotein E genes fed a low fat diet exhibit a constellation of pathologies shared with human atherosclerotic coronary heart disease ( CHD ) : hypercholesterolemia , occlusive coronary atherosclerosis , myocardial infarctions , cardiac dysfunction ( heart enlargement , reduced systolic function and ejection fraction , and ECG abnormalities ) , and premature death ( mean age 6 weeks ) . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| SR BI and APOE genotypes , anthropometric , clinical , biochemical , and lifestyle variables were determined . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| In this study the role of apoA 1 in SR BI mediated HDL CE selective uptake was tested via analyses of the biochemical properties of apoA 1 ( / ) HDL and its interaction with SR BI on adrenocortical cells , hepatoma cells , and cells expressing a transfected SR BI . apoA 1 ( / ) HDL are large heterogeneous particles with a core consisting predominantly of CE and a surface enriched in phospholipid , free cholesterol , apoA 2 , and apoE . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| By using a human adrenal cell line ( NCI H295R ) , we have addressed the role of apoE in binding to SR BI and in selective CE uptake from lipoproteins to cells . ^^^ This cell line does not secrete apoE and SR BI is its major HDL binding protein . ^^^ We can now provide evidence that 1 ) free apoE is a ligand for SR BI , 2 ) apoE associated to lipids or in lipoproteins does not modulate binding or CE selective uptake by the SR BI pathway , and 3 ) the direct interaction of free apoE to SR BI leads to an increase in CE uptake from lipoproteins of both low and high densities . ^^^ Lipid free apolipoprotein E binds to the class B Type 1 scavenger receptor 1 ( SR BI ) and enhances cholesteryl ester uptake from lipoproteins . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Apoe ( / ) and apoe ( + / + ) mice showed similar quantitative increases in LDL receptors , SR BI , adrenal weight gain , and ACAT activities in response to ACTH , and both genotypes had similar basal plasma ACTH concentrations . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| In this study , we investigated whether SR BI plays a role in the metabolism of cholesterol rich lipoprotein remnants that accumulate in apolipoprotein E ( apoE ) ( / ) mice . ^^^ To study SR BI activity in vivo , the receptor was overexpressed in apoE ( / ) mice by adenoviral vector mediated gene transfer . ^^^ To directly assess whether SR BI metabolizes apoE ( / ) mouse lipoprotein remnants , in vitro assays were performed in both CHO cells and primary hepatocytes expressing high levels of SR BI . ^^^ Taken together , these data establish that SR BI does not play a direct role in the metabolism of apoE ( / ) mouse lipoprotein remnants . . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| In the present investigation we report that ciprofibrate treatment causes the down regulation of hepatic scavenger receptor class B , type 1 ( SR BI ) protein expression in the livers of apoE deficient mice . ^^^ Restoration of SR BI expression in ciprofibrate treated apoE deficient mice by recombinant adenoviral gene transfer abolishes the ciprofibrate induced over accumulation of apoB 48 carrying remnants in the plasma . ^^^ We also report that remnants isolated from the plasma of ciprofibrate treated apoE deficient mice bind to murine SR BI expressed in stably transfected cultured cells . ^^^ These observations suggest that , in addition to its well established role as high density lipoprotein receptor , SR BI can also function as a remnant receptor responsible for the clearance of remnants from the circulation of apoE deficient mice . . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| However , changes in the plasma cholesterol concentration or loss of function of ATP binding cassette AI transporter ( ABCA 1 ) , scavenger receptor class B , type 1 ( SR BI ) , low density lipoprotein receptor ( LDLR ) , APOE or APOAI had no effect on sterol turnover in the brain . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| A number of proteins have been implicated in the process of promoting the efflux of free cholesterol from the macrophage , including apoE , ABCA 1 , and SRB 1 . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , elimination of SR BI in apolipoprotein E knockout mice results in rapid development of occlusive coronary artery disease , accompanied by spontaneous myocardial infarction , reduced heart function and early death , which points to a role for SR BI in protection against coronary heart disease . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Forty seven normolipidemic volunteers who were homozygous for the E 3 allele at the APOE gene were selected [ 37 homozygous for the common genotype ( 1 / 1 ) at the SR BI exon 1 polymorphism and 10 heterozygous ( 1 / 2 ) ] . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Scavenger receptor class B type 1 ( SR BI ) / apolipoprotein E ( apoE ) double knockout ( dKO ) mice exhibit many features of human coronary heart disease ( CHD ) , including occlusive coronary atherosclerosis , cardiac hypertrophy , myocardial infarctions , and premature death . ^^^ METHOD AND RESULTS : The lymphocyte deficient SR BI / apoE / recombination activating gene 2 ( RAG 2 ) triple knockout mice and corresponding dKO controls generated for this study exhibited essentially identical lipid rich coronary occlusions , myocardial infarctions , cardiac dysfunction , and premature death ( average lifespans 41 . 6+ / 0 . 6 and 42 . 0+ / 0 . 5 days , respectively ) . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Scavenger receptor class B , type 1 ( SR BI ) / ApoE double null mice develop severe atherosclerosis within 4 weeks , whereas ApoE null mice take several months to develop the disease , indicating that SR BI plays a pivotal role in atherosclerosis . ^^^ Importantly , SR BI / ApoE double null mice have lower plasma cholesterol levels than ApoE null mice , suggesting involvement of a non lipids mechanism . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Normal chow ( low fat ) fed mice deficient in both the HDL receptor SR BI and apolipoprotein E ( SR BI / apoE dKO ) provide a distinctive model of coronary heart disease ( CHD ) . ^^^ METHODS AND RESULTS : To generate a diet inducible model of CHD , we bred SR BI deficient ( SR BI KO ) mice with hypomorphic apolipoprotein E mice ( ApoeR 61 ( h / h ) ) that express reduced levels of an apoE 4 like murine apoE isoform and exhibit diet induced hypercholesterolemia . ^^^ When fed a normal chow diet , SR BI KO / ApoeR61 ( h / h ) mice did not exhibit early onset atherosclerosis or CHD ; the low expression level of the apoE 4 like murine apoE was atheroprotective and cardioprotective . ^^^ However , when fed an atherogenic diet rich in fat , cholesterol , and cholate , they rapidly developed hypercholesterolemia , atherosclerosis , and CHD , a response strikingly similar to that of SR BI / apoE dKO mice fed a chow diet , and they died 32+ / 6 days ( 50 % mortality ) after initiation of the high fat feeding . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| SR BI mediates cholesterol efflux via its interactions with lipid bound ApoE . ^^^ Apolipoprotein E ( apoE ) and the lipoprotein receptor SR BI play critical roles in lipid and lipoprotein metabolism . ^^^ We have examined the cholesterol efflux from wild type ( WT ) and mutant forms of SR BI expressed in ldlA 7 cells using reconstituted discoidal particles consisting of apoE , 1 palmitoyl 2 oleoyl l phospatidylcholine ( POPC ) , and cholesterol ( C ) as acceptors . ^^^ POPC / C apoE particles generated using apoE 2 , apoE 3 , apoE 4 , or carboxy terminally truncated forms apoE 4 165 , apoE 4 202 , apoE 4 229 , and apoE 4 259 caused similar ( 20 25 % ) cholesterol efflux from WT SR BI . ^^^ The rate of cholesterol efflux mediated by particles containing the WT or carboxy terminally truncated forms of apoE was decreased to approximately 30 % of the WT control with the Q402R / Q418R mutant SR BI form that is unable to bind native HDL normally but binds LDL . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Twelve male apoE ( / ) SR BI ( / ) mice fed 0 . 5 % probucol in a chow diet were lethally irradiated and transplanted with either wild type ( WT ) or DKO bone marrow . ^^^ Lipoprotein profiles and HDL subpopulations in WT > DKO mice were similar to apoE ( + / + ) SR BI ( / ) > DKO mice and resembled those of SR BI ( / ) mice . ^^^ CONCLUSIONS : Macrophage apoE is able to rescue the lethal phenotype of apoE ( / ) SR BI ( / ) mice by improving the dyslipidemia and dramatically reducing atherosclerotic lesion development . . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Our results show that treatment with rosiglitazone caused an increase in CD 36 mRNA and protein levels ( 2 . 7 and 2 . 9 fold , P < 0 . 001 ) , but significantly induced the expression of most genes related to cholesterol efflux : ABCA 1 mRNA ( 23 % , P < 0 . 05 ) and protein ( 2 . 4 fold , P < 0 . 05 ) , apo E protein ( 2 . 4 fold , P < 0 . 05 ) , caveolin 1 mRNA ( 2 . 6 fold , P < 0 . 001 ) and SR BI mRNA ( 1 . 9 fold , P < 0 . 001 ) and protein ( 3 fold , P < 0 . 01 ) . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| These oxidized phospholipids , either in the lipid phase of OxLDL or becoming attached covalently to apoprotein B during LDL oxidation , have been shown to play a major role in the binding of OxLDL to CD 36 and to SR B 1 expressed in transfected cells . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Relative expression of ABCA 1 , CETP , SR B 1 , EL , LPL , PLTP , ApoE and LDLR was investigated in HUVECs , human fibroblasts ( hFB ) and HepG 2 cells by quantitative real time PCR . ^^^ For CETP and EL mRNA expression , the results were HUVECs > hFB > HEPG 2 ; for PLTP , LDLR and LPL : hFB > HUVECs > HEPG 2 ; for SR B 1 and ApoE : HEPG 2 > HUVECs > hFB ; and for ABCA 1 HEPG 2 : > hFB > HUVECs . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Apolipoprotein E ( APOE ) is found in VLDL and binds to potential receptors involved in HCV entry into cells , the LDL receptor , and the scavenger receptor protein SR B 1 . ^^^ Apolipoprotein E ( APOE ) is found in VLDL and binds to potential receptors involved in HCV entry into cells , the LDL receptor , and the scavenger receptor protein SR B 1 . ^^^ |
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| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| Other gene products , such as apolipoprotein E ( apo E ) , scavenger receptor B 1 ( SR B 1 ) and acyl coenzyme : cholesterol acyltransferase 2 ( ACAT 2 ) affect cholesterol absorption also . ^^^ |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q8WTV0 and P02649 |
Pubmed |
SVM Score :0.0 |
| NA |
|