| INK4A , CDC 6 , and MCM 5 : predictive biomarkers in cervical preinvasive neoplasia and cervical cancer . ^^^ AIM : To analyse and compare expression patterns of three potential biomarkers p 16 ( INK4A ) , CDC 6 , and MCM 5 and evaluate their use as predictive biomarkers in squamous and glandular cervical preinvasive neoplasia . ^^^ METHODS : Immunocytochemical analysis of p 16 ( INK4A ) , MCM 5 , and CDC 6 expression was performed on 20 normal , 38 cervical intraepithelial neoplasia 1 ( CIN 1 ) , 33 CIN 2 , 46 CIN 3 , 10 squamous cell carcinoma , 19 cervical glandular intraepithelial neoplasia ( cGIN ) , and 10 adenocarcinoma samples . ^^^ Staining intensity was assessed using a 0 3 scoring system . p 16 ( INK4A ) , MCM 5 , and CDC 6 expression was also examined in ThinPrep slides exhibiting mild , moderate , and severe dyskaryosis . ^^^ RESULTS : All three markers showed a linear correlation between expression and grade of dysplasia . p 16 ( INK4A ) and MCM 5 protein expression was upregulated in all grades of squamous and glandular dysplasia . ^^^ |