| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| We have now isolated the human orthologues of rad 18 and spr 18 and designated them hSMC 6 and hSMC 5 . ^^^ The hSMC 6 and hSMC 5 proteins interact to form a tight complex analogous to the yeast Rad18 / Spr18 heterodimer . ^^^ Both hSMC 5 and hSMC 6 proteins are expressed at extremely high levels in the testis and associate with the sex chromosomes in the late stages of meiotic prophase , suggesting a possible role for these proteins in meiosis . . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| The SMC 2 SMC4 and SMC 1 SMC3 complexes are also known as condensin and cohesin , respectively , but the recently identified SMC 5 and SMC 6 complex is less well characterized . ^^^ Here , we report that NSE 1 from Saccharomyces cerevisiae encodes a novel non SMC component of the SMC 5 ( Yol034wp ) SMC 6 ( Rhc18p ) complex corresponding to the 2 3 MDa molecular mass . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| Genetic and biochemical studies indicate that Rad 60 functions codependently with Smc 5 and Smc 6 , subunits of an SMC ( structural maintenance of chromosomes ) complex required for recombinational repair . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| SMC heterodimers are required for sister chromatid cohesion ( Cohesin : Smc 1 & Smc 3 ) , chromatin condensation ( Condensin : Smc 2 & Smc 4 ) , and DNA repair ( Smc 5 & Smc 6 ) . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that the SMC 5 and SMC 6 subfamily members have evolved comparatively rapidly and suggest that these proteins may perform redundant functions in higher eukaryotes . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| Smc 5 and Smc 6 proteins form a heterodimeric SMC ( structural maintenance of chromosome ) protein complex like SMC 1 SMC3 cohesin and SMC 2 SMC4 condensin , and they associate with non SMC proteins Nse 1 and Nse 2 stably and Rad 60 transiently . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| Rad 18 ( Smc 6 ) is a member of the structural maintenance of chromosomes ( SMC ) family and , together with its SMC partner Spr 18 ( Smc 5 ) , forms the core of a high molecular weight complex . ^^^ We show here that both S . pombe and human Smc 5 and 6 interact through their hinge domains and that four independent temperature sensitive mutants of Rad 18 ( Smc 6 ) are all mutated at the same glycine residue in the hinge region . ^^^ This mutation abolishes the interactions between the hinge regions of Rad 18 ( Smc 6 ) and Spr 18 ( Smc 5 ) , as does mutation of a conserved glycine in the hinge region of Spr 18 ( Smc 5 ) . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| The Schizosaccharomyces pombe SMC proteins Rad 18 ( Smc 6 ) and Spr 18 ( Smc 5 ) exist in a high M ( r ) complex which also contains the non SMC proteins Nse 1 , Nse 2 , Nse 3 , and Rad 62 . ^^^ Smc 6 ( Rad 18 ) and Nse 3 , but not Smc 5 ( Spr 18 ) or Nse 1 , are sumoylated in vitro in an Nse 2 dependent manner , and Nse 2 is itself autosumoylated , predominantly on the C terminal part of the protein . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| Among the subunits are Mms 21 that contains a putative Siz / PIAS ( protein inhibitor of activated signal transducer and activator of transcription ) RING domain characteristic of small ubiquitin like modifier ( SUMO ) ligases , two structural maintenance of chromosome ( Smc ) proteins , Smc 5 and Smc 6 , and a protein that contains an ubiquitin ligase signature domain . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| SMC 5 and SMC 6 genes are required for the segregation of repetitive chromosome regions . ^^^ Here , we generated conditional mutants of SMC 5 and SMC 6 in budding yeast , in which the essential function was affected . ^^^ We show that mutant smc 5 6 and smc 6 9 cells undergo an aberrant mitosis in which chromosome segregation of repetitive regions is impaired ; this leads to DNA damage and RAD 9 dependent activation of the Rad 53 protein kinase . ^^^ Consistent with a requirement for the segregation of repetitive regions , Smc 5 and Smc 6 proteins are enriched at ribosomal DNA ( rDNA ) and at some telomeres . ^^^ Furthermore , deletion of RAD 52 partially suppresses the temperature sensitivity of smc 5 6 and smc 6 9 mutants . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| Another complex containing SMC 5 and SMC 6 is implicated in DNA repair and checkpoint responses . ^^^ |
|
| Interacting proteins: Q8IY18 and Q96SB8 |
Pubmed |
SVM Score :0.0 |
| In yeasts , a multisubunit complex consisting of SMC 5 , SMC 6 , MMS21 / NSE2 , and other non SMC proteins is required for DNA repair through homologous recombination . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| We have now isolated the human orthologues of rad 18 and spr 18 and designated them hSMC 6 and hSMC 5 . ^^^ The hSMC 6 and hSMC 5 proteins interact to form a tight complex analogous to the yeast Rad18 / Spr18 heterodimer . ^^^ Both hSMC 5 and hSMC 6 proteins are expressed at extremely high levels in the testis and associate with the sex chromosomes in the late stages of meiotic prophase , suggesting a possible role for these proteins in meiosis . . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| Smc 5 and Smc 6 proteins form a heterodimeric SMC ( structural maintenance of chromosome ) protein complex like SMC 1 SMC3 cohesin and SMC 2 SMC4 condensin , and they associate with non SMC proteins Nse 1 and Nse 2 stably and Rad 60 transiently . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| Rad 18 ( Smc 6 ) is a member of the structural maintenance of chromosomes ( SMC ) family and , together with its SMC partner Spr 18 ( Smc 5 ) , forms the core of a high molecular weight complex . ^^^ We show here that both S . pombe and human Smc 5 and 6 interact through their hinge domains and that four independent temperature sensitive mutants of Rad 18 ( Smc 6 ) are all mutated at the same glycine residue in the hinge region . ^^^ This mutation abolishes the interactions between the hinge regions of Rad 18 ( Smc 6 ) and Spr 18 ( Smc 5 ) , as does mutation of a conserved glycine in the hinge region of Spr 18 ( Smc 5 ) . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| The Schizosaccharomyces pombe SMC proteins Rad 18 ( Smc 6 ) and Spr 18 ( Smc 5 ) exist in a high M ( r ) complex which also contains the non SMC proteins Nse 1 , Nse 2 , Nse 3 , and Rad 62 . ^^^ Smc 6 ( Rad 18 ) and Nse 3 , but not Smc 5 ( Spr 18 ) or Nse 1 , are sumoylated in vitro in an Nse 2 dependent manner , and Nse 2 is itself autosumoylated , predominantly on the C terminal part of the protein . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| Among the subunits are Mms 21 that contains a putative Siz / PIAS ( protein inhibitor of activated signal transducer and activator of transcription ) RING domain characteristic of small ubiquitin like modifier ( SUMO ) ligases , two structural maintenance of chromosome ( Smc ) proteins , Smc 5 and Smc 6 , and a protein that contains an ubiquitin ligase signature domain . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| The SMC 2 SMC4 and SMC 1 SMC3 complexes are also known as condensin and cohesin , respectively , but the recently identified SMC 5 and SMC 6 complex is less well characterized . ^^^ Here , we report that NSE 1 from Saccharomyces cerevisiae encodes a novel non SMC component of the SMC 5 ( Yol034wp ) SMC 6 ( Rhc18p ) complex corresponding to the 2 3 MDa molecular mass . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| Genetic and biochemical studies indicate that Rad 60 functions codependently with Smc 5 and Smc 6 , subunits of an SMC ( structural maintenance of chromosomes ) complex required for recombinational repair . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| SMC heterodimers are required for sister chromatid cohesion ( Cohesin : Smc 1 & Smc 3 ) , chromatin condensation ( Condensin : Smc 2 & Smc 4 ) , and DNA repair ( Smc 5 & Smc 6 ) . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that the SMC 5 and SMC 6 subfamily members have evolved comparatively rapidly and suggest that these proteins may perform redundant functions in higher eukaryotes . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| In yeasts , a multisubunit complex consisting of SMC 5 , SMC 6 , MMS21 / NSE2 , and other non SMC proteins is required for DNA repair through homologous recombination . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| SMC 5 and SMC 6 genes are required for the segregation of repetitive chromosome regions . ^^^ Here , we generated conditional mutants of SMC 5 and SMC 6 in budding yeast , in which the essential function was affected . ^^^ We show that mutant smc 5 6 and smc 6 9 cells undergo an aberrant mitosis in which chromosome segregation of repetitive regions is impaired ; this leads to DNA damage and RAD 9 dependent activation of the Rad 53 protein kinase . ^^^ Consistent with a requirement for the segregation of repetitive regions , Smc 5 and Smc 6 proteins are enriched at ribosomal DNA ( rDNA ) and at some telomeres . ^^^ Furthermore , deletion of RAD 52 partially suppresses the temperature sensitivity of smc 5 6 and smc 6 9 mutants . ^^^ |
|
| Interacting proteins: Q96SB8 and Q8IY18 |
Pubmed |
SVM Score :0.0 |
| Another complex containing SMC 5 and SMC 6 is implicated in DNA repair and checkpoint responses . ^^^ |
|