| A death associated protein kinase ( DAPK ) interacting protein , DIP 1 , is an E 3 ubiquitin ligase that promotes tumor necrosis factor induced apoptosis and regulates the cellular levels of DAPK . ^^^ In these studies we have identified a DAPK interacting protein called DIP 1 , which is a novel multi RING finger protein . ^^^ Transient expression of DIP 1 in HeLa cells antagonizes the anti apoptotic function of DAPK to promote a caspase dependent apoptosis . ^^^ These studies also demonstrate that DAPK is an in vitro and in vivo target for ubiquitination by DIP 1 , thereby providing a mechanism by which DAPK activities can be regulated through proteasomal degradation . . ^^^ |