| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| This factor , referred to as LMO 4 , is expressed in overlapping manner with Clim 2 in epidermis and in several other regions , including epithelial cells of the gastrointestinal , respiratory and genitourinary tracts , developing cartilage , pituitary gland , and discrete regions of the central and peripheral nervous system . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| The LMO coactivator Clim2 / Ldb1 / NLI showed a similar expression pattern , consistent with the idea that LMO 4 and Clim 2 act as a complex in mammary epithelial cells . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation experiments indicate that LMO 4 can form a complex with cAMP response element binding protein ( CREB ) and can interact with cofactor of LIM homeodomain protein 1 ( CLIM 1 ) and CLIM 2 . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| We have now identified a new member of the Lmo family , designated Lmo 4 , via its interaction with Ldb 1 . ^^^ These characteristics imply that Ldb 1 Lmo4 interaction may function in the T cell developmental programme and that enforced expression of LMO 1 or LMO 2 by chromosomal translocations or transgenesis may displace Lmo 4 from this complex and thereby influence T cell differentiation prior to T cell tumour occurrence . . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| A new member of the Lmo family , Lmo 4 , has also recently been identified via its interaction with Ldb 1 . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| H , 15N and 13C assignments of FLIN 4 , an intramolecular LMO 4 : ldb 1 complex . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| Structural basis for the recognition of ldb 1 by the N terminal LIM domains of LMO 2 and LMO 4 . ^^^ LMO 2 and LMO 4 interact with the ubiquitous nuclear adaptor protein ldb1 / NLI / CLIM2 , which associates with the LIM domains of LMO and LIM homeodomain proteins via its LIM interaction domain ( ldb 1 LID ) . ^^^ We report the solution structures of two LMO : ldb 1 complexes ( PDB : 1M3V and 1J2O ) and show that ldb 1 LID binds to the N terminal LIM domain ( LIM 1 ) of LMO 2 and LMO 4 in an extended conformation , contributing a third strand to a beta hairpin in LIM 1 domains . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| The LIM only protein , LMO 4 , and the LIM domain binding protein , LDB 1 , expression in squamous cell carcinomas of the oral cavity . ^^^ LMO 4 mRNA was amplified in four of six carcinoma tissues and eight of 12 carcinoma cell lines , and LDB 1 in three carcinoma tissues and 11 cell lines examined . ^^^ Immunoprecipitation studies revealed that LMO 4 and LDB 1 interact with each other in the nuclear milieu of the carcinoma cells indicating the presence of an LMO 4 LDB1 mediated transcription complex . ^^^ Both LMO 4 and LDB 1 proteins were preferentially localised in the nuclei of carcinoma cells at the invasive front and the immunoreactivity was increased in less differentiated carcinoma tissues ( P < 0 . 01 ) . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| LMO 4 is comprised primarily of two tandemly repeated LIM domains and interacts with the ubiquitous nuclear adaptor protein ldb 1 . ^^^ This interaction is mediated via the LIM domains of LMO 4 and the LIM interaction domain ( LID ) of ldb 1 . ^^^ An intramolecular complex , termed FLINC 4 , consisting of the two LIM domains from LMO 4 linked to the LID domain of ldb 1 via a flexible linker has been engineered , purified and crystallized . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| Tandem LIM domains provide synergistic binding in the LMO 4 : Ldb 1 complex . ^^^ We have determined a high resolution 10 ray crystal structure of LMO 4 , a putative breast oncoprotein , in complex with Ldb 1 LID , providing the first example of a tandem LIM : Ldb 1 LID complex and the first structure of a type B LIM domain . ^^^ The complex possesses a highly modular structure with Ldb 1 LID binding in an extended manner across both LIM domains of LMO 4 . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| We next applied the biotinylation tag to Ldb 1 , a known partner of GATA 1 , and characterized a number of novel interaction partners that are essential in erythroid development , in particular , Eto 2 , Lmo 4 , and CdK 9 . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| The tumor suppressor LKB 1 induces p 21 expression in collaboration with LMO 4 , GATA 6 , and Ldb 1 . ^^^ We show here that LKB 1 forms a complex with LMO 4 , GATA 6 , and Ldb 1 , and enhances GATA mediated transactivation in a kinase dependent manner . ^^^ We further demonstrate that LKB 1 has the potential to induce p 21 expression in collaboration with LMO 4 , GATA 6 , and Ldb 1 through the p 53 independent mechanism . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| Sequestration of Ldb 1 by LMO 2 in T cells may prevent it binding other key partners , such as LMO 4 . ^^^ Here , we show using protein engineering and enzyme linked immunosorbent assay ( ELISA ) methodologies that LMO 2 binds Ldb 1 with a twofold lower affinity than does LMO 4 . ^^^ The delineation of this region reveals some specific differences when compared to the equivalent LMO 4 : Ldb 1 interaction that hold promise for the development of reagents to specifically bind LMO 2 in the treatment of leukemia . . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| LMO 1 , LMO 2 , and LMO 4 have distinct expression patterns in adult tissue , and we demonstrate that nuclear retention of LMO proteins is enhanced by the nuclear LIM interactor ( NLI ) . ^^^ In situ hybridization to early mouse embryos of 8 14 . 5 days revealed a complex pattern of LMO 4 expression spatially overlapping with NLI and LHX genes . ^^^ |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q86U70 and P61968 |
Pubmed |
SVM Score :0.0 |
| NA |
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