| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :1.1758576 |
| LMO 2 comprises two protein binding LIM domains that allow LMO 2 to interact with multiple protein partners , including LIM domain binding protein 1 ( Ldb 1 , also known as CLIM 2 and NLI ) , an essential cofactor for LMO proteins . 1.1758576^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.5263927 |
| LMO 2 and LMO 4 interact with the ubiquitous nuclear adaptor protein ldb1 / NLI / CLIM2 , which associates with the LIM domains of LMO and LIM homeodomain proteins via its LIM interaction domain ( ldb 1 LID ) . 0.5263927^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| These data demonstrate one function for the LIM binding protein Ldb 1 and establish a function for the LIM only protein Lmo 2 as an obligatory component of an oligomeric , DNA binding complex which may play a role in haematopoiesis . . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| The LIM domain binding protein Ldb 1 and its partner LMO 2 act as negative regulators of erythroid differentiation . ^^^ We , and others , have identified a widely expressed protein , Ldb 1 , whose C terminal 76 residues are sufficient to mediate interaction with LMO 2 . ^^^ However , Ldb 1 , LMO 2 , and SCL / E12 can assemble as a multiprotein complex on a consensus SCL binding site . ^^^ Like LMO 2 , the Ldb 1 gene is expressed in fetal liver and erythroid cell lines . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| These characteristics imply that Ldb 1 Lmo4 interaction may function in the T cell developmental programme and that enforced expression of LMO 1 or LMO 2 by chromosomal translocations or transgenesis may displace Lmo 4 from this complex and thereby influence T cell differentiation prior to T cell tumour occurrence . . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| The composite motif binds a multi protein hematopoietic specific transcription factor complex which includes GATA 1 , SCL / tal 1 , E2A , Lmo 2 and Ldb 1 . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| The oncogenic functions of LMO 1 and LMO 2 are thought to be mediated by interaction with LDB 1 since they form a multimeric protein complex ( es ) . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| Human proerythroblasts and early erythroblasts , generated in vitro by normal adult progenitors , contain a pentamer protein complex comprising the tal 1 transcription factor heterodimerized with the ubiquitous E2A protein and linked to Lmo 2 , Ldb 1 , and retinoblastoma protein ( pRb ) . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| Therefore , we have designed and constructed a fusion protein in which ldb 1 ( LID ) and LIM 1 of LMO 2 can form an intramolecular complex . ^^^ The engineered protein , FLIN 2 ( fusion of the LIM interacting domain of ldb 1 and the N terminal LIM domain of LMO 2 ) has been expressed and purified in milligram quantities . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| H , 15N and 13C assignments of FLIN 2 , an intramolecular LMO 2 : ldb 1 complex . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| This complex , containing hematopoietic specific ( SCL , Lim only 2 ( LMO 2 ) , GATA 1 / GATA 2 ) and ubiquitous ( E2A , LIM domain binding protein 1 [ Ldb 1 ] ) factors , is tethered to DNA via a specificity protein 1 ( Sp 1 ) motif , through direct interactions between elements of the SCL complex and the Sp 1 zinc finger protein . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| We show here that a nuclear complex containing TAL 1 , its DNA binding partner E 47 , zinc finger transcription factor GATA 1 , LIM domain protein LMO 2 , and LIM domain binding protein Ldb 1 transactivates the protein 4 . 2 ( P4 . 2 ) gene through two E box GATA elements in its proximal promoter . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| GPA promoter activation is dependent on the assembly of a multifactorial complex containing SCL as well as ubiquitous ( E 47 , Sp 1 , and Ldb 1 ) and tissue specific ( LMO 2 and GATA 1 ) transcription factors . ^^^ Finally , endogenous GPA expression is disrupted in hematopoietic cells through the dominant inhibitory effect of a truncated form of E 47 ( E 47 bHLH ) on E protein activity or of FOG ( Friend of GATA ) on GATA activity or when LMO 2 or Ldb 1 protein levels are decreased . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| In vivo , they both bind the transcription factors GATA 1 , SCL , LMO 2 , and Ldb 1 . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| To gain information on the possible involvement of this transcription factor in the non responsiveness of acute myelocytic leukemia ( AML ) patients to ATRA , we compared the endogenous expression levels of Scl and its transcription complex partners [ i . e . , Rbtn 1 ( LMO 1 ) , Rbtn 2 ( LMO 2 ) , Ldb 1 , and GATA family proteins ] in leukemic blast cells from patients with AML and acute promyelocytic leukemia ( APL ) , and determined the effects of lithium chloride alone or in combination with ATRA on the capacity of blast cells to differentiate during short term ex vivo culture . ^^^ Levels of Scl , Rbtn 2 , GATA 1 , and Ldb 1 expression were comparable in AML and APL blasts , while the levels of expression of Rbtn 1 , GATA 2 , and GATA 3 were absent or markedly lower in APL cells . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : The LIM finger protein LMO 2 forms a transcription factor complex with other hematopoietic regulator proteins , such as TAL 1 ( SCL ) , LDB 1 , GATA 1 , 2 , and 3 , in the promoters of several erythroid genes . ^^^ MATERIALS AND METHODS : Protein interactions of LIM modified LMO 2 constructs with TAL 1 , LDB 1 , and GATAs were examined in an immunoprecipitation assay . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| In addition to known TAL 1 partners , GATA 1 , E2A , HEB , LMO 2 and Ldb 1 , we identify the ETO 2 repressor as a novel component recruited to TAL 1 complexes through interaction with E2A / HEB . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| Quantitative real time polymerase chain reaction showed that Scl null platelets lacked NF E 2 , and chromatin immunoprecipitation analysis demonstrated Scl binding to the NF E 2 promoter in the human megakaryoblastic cell line Meg 01 , along with its binding partners E 47 , Lmo 2 , and the cofactors Ldb 1 and GATA 2 . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| We recently established that a DNA binding complex containing TAL1 / SCL , E 47 , GATA 1 , LMO 2 and Ldb 1 stimulates P4 . 2 ( protein 4 . 2 ) transcription in erythroid progenitors via two E box GATA elements in the gene ' s proximal promoter . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| We also found that mouse Ldb1b lacks the ability to activate protein 4 . 2 promoter , which is stimulated by LMO 2 and Ldb 1 . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| LMO 1 , LMO 2 , and LMO 4 have distinct expression patterns in adult tissue , and we demonstrate that nuclear retention of LMO proteins is enhanced by the nuclear LIM interactor ( NLI ) . ^^^ |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q86U70 and P25791 |
Pubmed |
SVM Score :0.0 |
| NA |
|