| UNC5H1 induces apoptosis via its juxtamembrane region through an interaction with NRAGE . ^^^ Here , we report an interaction between UNC5H1 and NRAGE . ^^^ We mapped the NRAGE binding domain of UNC5H1 to its ZU 5 domain and show that this region , in addition to an adjacent PEST sequence , is required for UNC5H1 mediated apoptosis . ^^^ Chimeric UNC5H2 and UNC5H3 receptors , containing the NRAGE binding domain and PEST sequence of UNC5H1 , bind NRAGE and cause increased levels of apoptosis . ^^^ UNC5H1 expression does not induce apoptosis in differentiated PC 12 cells , which down regulate NRAGE , but induces apoptosis in native PC 12 cells that endogenously express high levels of NRAGE and in differentiated PC 12 cells when NRAGE is overexpressed . ^^^ |