| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| Assembly of native FN induced a rapid reorganization of actin into stress fibers and colocalization of alpha 5 beta 1 integrin , focal adhesion kinase ( FAK ) , vinculin , and paxillin to regions of cell matrix contact . alpha 5 beta 1 integrins and FAK are also clustered upon binding of FN delta III 1 7 to cells but actin reorganization and focal adhesion formation are delayed and appear to be dependent on the formation of FN delta III 1 7 fibrils . ^^^ |
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| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| While overexpression of Csk or the Csk mutants did not significantly affect cell adhesion to the Fn surface or alpha 5 integrin recruitment to the attachment sites , Csk suppressed and mCsk almost abolished Fn mediated tyrosine phosphorylation of paxillin , filamentous actin assembly to podosomes , and cell migration , but mCsk ( ) did not . ^^^ |
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| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that FAK and MAP kinase belong to distinct integrin signaling pathways and that both pathways remain functional during Caco 2 cell differentiation since the coating of differentiating cells on FN and LN but not on polylysine increased the tyrosine phosphorylation of FAK and of its endogenous substrate paxillin , and stimulated MAP kinase activity . ^^^ |
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| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| Expression of various FAK mutants in the FAK cells showed that FAK kinase activity , the Tyr 397 / SH2 domain binding site , and the first proline rich SH 3 binding region in the FAK C terminal domain were individually needed to promote full FAK mediated FAK cell migration to FN whereas direct paxillin binding to FAK was not required . ^^^ |
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| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| LPA and FN also evoked the formation of focal adhesions and actin bundles , and tyrosine phosphorylation of focal adhesion kinase ( FAK ) and paxillin . ^^^ |
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| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| Here , endothelial GM 7373 cells spread and organize focal adhesion plaques on immobilized FGF 2 , fibronectin ( FN ) , and vitronectin ( VN ) . alpha ( 5 ) beta ( 3 ) integrin , paxillin , focal adhesion kinase , vinculin and pp 60 ( src ) localize in cell substratum contact sites on FGF 2 , FN or VN . ^^^ |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| These results indicate that IFN mediated loss of monocyte adhesion to Fn is associated with changes in the cytoskeleton associated proteins paxillin and Rho . . ^^^ |
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| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| Integrin activation by immobilized FN induced phosphorylation of pp 125 focal adhesion kinase and paxillin and induced the formation of focal adhesion kinase / Grb 2 / Sos complex . ^^^ |
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| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| Collectively , these data suggest CadF promotes the binding of C . jejuni to Fn , which in turn stimulates a signal transduction pathway involving paxillin . . ^^^ |
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| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| As a result of FN binding by the beta 1EhFNR , the receptor itself , FAK , and paxillin were phosphorylated in tyrosine . ^^^ Co immunoprecipitation experiments showed that a multimolecular signaling complex was formed by the amoebic FN receptor , FAK , paxillin , and vinculin . ^^^ |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| In contrast , the focal adhesion protein paxillin was not phosphorylated at Tyr 31 with FN . ^^^ |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| RESULTS : CN binding to integrins can mimic the intracellular signaling cascade evoked by FN , because the phosphorylation of the key signaling proteins focal adhesion kinase , paxillin , and p 130 Crk associated substrate and the association of Src with focal adhesion kinase are similar . ^^^ |
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| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| Consequently , tyrosine phosphorylation of paxillin was obviously persistent after stimulation with FN + LPA as compared to after stimulation with either alone . ^^^ Immunoblotting of anti paxillin immunoprecipitates with phosphorylation site specific Abs revealed the following : tyrosine phosphorylation was enhanced preferentially on a slowly migrating component after stimulation with FN + LPA ; this component contained phosphorylation at both tyrosine residue ( Y ) 31 and Y 118 ; and phosphorylation of paxillin at Y 181 was constitutive and not augmented by stimulation with either FN or LPA . ^^^ Paxillin phosphorylation weakly induced by FN alone , insufficient for cell migration , was not inhibited by amiloride . ^^^ These results demonstrate that LPA collaborates with FN for persistent tyrosine phosphorylation of paxillin at both Y 31 and Y 118 , regulated by the Na+ / H+ antiporter downstream of ROCK and that this phosphorylated paxillin is essential for MM 1 cancer cell migration . . ^^^ |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| Of the LIM domains , LIM 2 and 3 comprise the paxillin FA targeting motif , with phosphorylation of these domains modulating paxillin targeting and cell adhesion to fibronectin ( Fn ) . ^^^ |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q6MZF4 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|