| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.60790269 |
| ACT is expressed coordinately with CREM in a tissue and developmentally regulated manner . 0.60790269^^^ Using a two hybrid screen , we have isolated a testis derived complementary DNA encoding a protein that we term ACT ( for activator of CREM in testis ) , a LIM only protein which specifically associates with CREM . 0.55762735^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.51288411 |
| ACT is a LIM only protein which associates with CREM in male germ cells and provides an activation function which is independent of phosphorylation and CBP . 0.51288411^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Recent results have shown that CREM needs a tissue specific co activator , ACT ( activator of CREM in testis ) to elicit its regulatory function in testis . . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Cloning and expression of activator of CREM in testis in human testicular tissue . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| The molecular mechanism by which CREM elicits its regulatory function involves ACT ( Activator of CREM in Testis ) , a testis specific coactivator constituted by a repeat of four and half LIM domains . ^^^ |
|
| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Furthermore , mRNAs of ACT ( activator of CREM in testis ) and CREM target genes are downregulated in miwi ( null ) testes . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| In testis , CREM transcriptional activity is controlled through interaction with a tissue specific partner , ACT ( activator of CREM in testis ) , which confers a powerful , phosphorylation independent activation capacity . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Microtubule independent and protein kinase A mediated function of kinesin KIF17b controls the intracellular transport of activator of CREM in testis ( ACT ) . ^^^ The testis specific kinesin KIF17b was shown to directly regulate cAMP response element modulator ( CREM ) dependent transcription by determining the subcellular localization of the activator of CREM in testis ( ACT ) , the testis specific coactivator of CREM in postmeiotic male germ cells . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| The cyclic adenosine monophosphate ( cAMP ) response element modulator ( CREM ) is an essential component of this program , and its activity is regulated through interactions with a germ cell specific , CREM phosphorylation independent transcriptional co activator , activator of CREM in testis ( ACT ) . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Mechanisms of activation by CREB and CREM : phosphorylation , CBP , and a novel coactivator , ACT . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| For example , in male germ cells CREM is not phosphorylated but associates with ACT , a member of the LIM only class of proteins that has intrinsic transcriptional activity . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Activation might also be independent of CBP and phosphorylation in some specific cell types , such as male germ cells , wherein the protein ACT confers a powerful activation function to CREM . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Interestingly , in male germ cells , CREM is not phosphorylated but associates with ACT , a member of the LIM only class of proteins that has intrinsic transcriptional activity . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| ACT is a LIM only protein expressed exclusively in round spermatids , where it cooperates with transcriptional activator CREM in regulating various postmeiotic genes . ^^^ We sought to identify the regulatory steps controlling the functional interplay between CREM and ACT . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Expression of activator of CREM in the testis ( ACT ) during normal and impaired spermatogenesis : correlation with CREM expression . ^^^ Transcriptional activity of the CREM protein is thought to be regulated by activator of CREM in the testis ( ACT ) . ^^^ In addition , CREM knockout mice known to be infertile due to RSMA also exhibited only a weak amplification product for ACT cDNA . ^^^ Our findings indicate a conserved function of ACT during the evolution of mammalian spermatogenesis and suggest a role for CREM in ACT transcriptional regulation . . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| In testis , CREM transcriptional activity is controlled through interaction with a tissue specific partner , ACT , which confers a powerful , phosphorylation independent activation capacity . ^^^ |
|
| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Because KIF17b has been reported to control CREM dependent transcription in male germ cells by regulating the intracellular location of the transcriptional coactivator activator of CREM in testis , this indicates that one kinesin links the processes of transcription and transport of specific mRNAs in mammalian male germ cells . . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| In testis , CREM transcriptional activity is controlled through interaction with a tissue specific partner , activator of CREM in the testis ( ACT ) , which confers a powerful , phosphorylation independent activation capacity . ^^^ |
|
| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Targeted inactivation of the genes encoding CREM and ACT has been achieved . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| Sequencing and haplotype analysis of the activator of CREM in the testis ( ACT ) gene in populations of fertile and infertile males . cAMP responsive element modulator ( CREM ) is a key transcription factor in the differentiation of round spermatids into mature spermatozoa . ^^^ During spermiogenesis , CREM is regulated in part by activator of CREM in the testis ( ACT ) , which activates CREM in a phosphorylation independent fashion . ^^^ To test whether the different haplotypes conferred a functional change to the ACT protein , a yeast two hybrid assay was designed to test the interaction between the two most divergent ACT haplotypes and their known binding partners , CREM and KIF17b . ^^^ We identified one ACT haplotype that had a 45 % reduction in its interaction with CREM . ^^^ |
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| Interacting proteins: Q5TD97 and Q03060 |
Pubmed |
SVM Score :0.0 |
| NA |
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