| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| In vivo , Mex67p requires Mtr2p for association with the nuclear pores , which can be abolished by mutating either MEX 67 or MTR 2 . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| MEX 67 and MTR 2 also genetically interact with different types of repeat nucleoporins , such as Nup116p , Nup159p , Nsp1p , and Rip1p / Nup40p . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| Further investigation of a putative functional relationship between mRNA metabolism and THO revealed that mRNA export mutants sub 2 , yra 1 , mex 67 and mtr 2 have similar defective transcription and hyper recombination phenotypes as THO mutants . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| Mex 67 , along with its cofactor Mtr 2 , is thought to promote ribonucleoparticle translocation by interacting directly with components of the nuclear pore complex ( NPC ) . ^^^ Furthermore , Sac 3 can be coimmunoprecipitated with Mex 67 , Mtr 2 , and other factors involved in mRNA export . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| The association between Mtr 2 and Mex 67 is essential for the nuclear export of bulk messenger RNA in yeast . ^^^ Whereas Mex 67 and TAP are highly conserved proteins , their binding partners , Mtr 2 and p 15 , share no sequence similarity , but are nevertheless functionally homologous . ^^^ Here , we report the 2 . 8 A resolution crystal structure of Mtr 2 in complex with the NTF 2 like domain of Mex 67 . ^^^ Mtr 2 is a novel member of the NTF 2 like family and interacts with Mex 67 , forming a complex with a similar structural architecture to that of TAP p 15 . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| We report the crystal structures of apo Mtr 2 from the human pathogen Candida albicans and of its complex with the Mex 67 NTF2 like domain . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| The TAP protein includes a noncanonical RNP type RNA binding domain , four leucine rich repeats , an NTF 2 like domain that allows heterodimerization with p 15 ( also called NXT 1 ) , and a ubiquitin associated domain that mediates the interaction with nucleoporins . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| NXT 1 ( p 15 ) is a crucial cellular cofactor in TAP dependent export of intron containing RNA in mammalian cells . ^^^ Significantly , cotransfection with a vector expressing NXT 1 ( p 15 ) , an NTF 2 related cellular factor that binds to TAP , led to dramatic enhancement of the ability of the chimeric proteins to mediate RNA export . ^^^ Mutant protein analysis demonstrated that the domain necessary for nuclear export mapped to the C terminal region of TAP and required the domain that interacts with NXT 1 , as well as the region that has been shown to interact with nucleoporins . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| RNA export mediated by tap involves NXT 1 dependent interactions with the nuclear pore complex . ^^^ Here , we have characterized the function of NXT 1 in the context of the Tap dependent RNA export pathway . ^^^ We demonstrate that NXT 1 stimulates binding of a Tap RNA complex to nucleoporins in vitro , and we provide mutational analysis that shows these interactions are necessary for nuclear export of an intron containing viral mRNA in vivo . ^^^ Tap contains separate domains for binding to nucleoporins and NXT 1 , both of which are critical for its export function . ^^^ RNA export is mediated by a heterodimer of Tap and NXT 1 , and the function of NXT 1 on this pathway is to regulate the affinity of the Tap RNA complex for nucleoporins within the nuclear pore complex . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| In the absence of NXT 1 binding , the Tap protein is unable to effectively interact with components of the nuclear pore complex and both Tap nucleocytoplasmic shuttling and the nuclear export of mRNA molecules tethered to Tap are therefore severely attenuated . ^^^ These data suggest that NXT 1 may act as a molecular switch that regulates the ability of Tap to mediate nuclear mRNA export by controlling the interaction of Tap with components of the nuclear pore . . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| Exogeneous expression of Tap and NXT 1 was necessary and sufficient to rescue Gag augmentation in 293 cells . ^^^ Overexpression experiments established that CTE , but not RU 5 , confers the responsiveness to Tap and NXT 1 and that CTE in conjunction with Tap and NXT 1 conferred a 30 fold increase in translational utilization of the cytoplasmic RNA . ^^^ Our results uncovered a previously unidentified role of CTE in conjunction with Tap and NXT 1 in commitment to efficient cytoplasmic RNA utilization . . ^^^ |
|
| Interacting proteins: Q59E96 and Q9UKK6 |
Pubmed |
SVM Score :0.0 |
| The Tap RNA complex was shown to bind to three nucleoporins , Nup 98 , p 62 , and RanBP 2 , and these interactions were enhanced by Nxt 1 . ^^^ Mutations in the Tap UBA region abolished interactions with all three nucleoporins , whereas the effect of point mutations within the NTF 2 like domain of Tap known to disrupt Nxt 1 binding or nucleoporin binding were nucleoporin dependent . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| In vivo , Mex67p requires Mtr2p for association with the nuclear pores , which can be abolished by mutating either MEX 67 or MTR 2 . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| MEX 67 and MTR 2 also genetically interact with different types of repeat nucleoporins , such as Nup116p , Nup159p , Nsp1p , and Rip1p / Nup40p . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| Further investigation of a putative functional relationship between mRNA metabolism and THO revealed that mRNA export mutants sub 2 , yra 1 , mex 67 and mtr 2 have similar defective transcription and hyper recombination phenotypes as THO mutants . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| Mex 67 , along with its cofactor Mtr 2 , is thought to promote ribonucleoparticle translocation by interacting directly with components of the nuclear pore complex ( NPC ) . ^^^ Furthermore , Sac 3 can be coimmunoprecipitated with Mex 67 , Mtr 2 , and other factors involved in mRNA export . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| The association between Mtr 2 and Mex 67 is essential for the nuclear export of bulk messenger RNA in yeast . ^^^ Whereas Mex 67 and TAP are highly conserved proteins , their binding partners , Mtr 2 and p 15 , share no sequence similarity , but are nevertheless functionally homologous . ^^^ Here , we report the 2 . 8 A resolution crystal structure of Mtr 2 in complex with the NTF 2 like domain of Mex 67 . ^^^ Mtr 2 is a novel member of the NTF 2 like family and interacts with Mex 67 , forming a complex with a similar structural architecture to that of TAP p 15 . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| We report the crystal structures of apo Mtr 2 from the human pathogen Candida albicans and of its complex with the Mex 67 NTF2 like domain . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| The TAP protein includes a noncanonical RNP type RNA binding domain , four leucine rich repeats , an NTF 2 like domain that allows heterodimerization with p 15 ( also called NXT 1 ) , and a ubiquitin associated domain that mediates the interaction with nucleoporins . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| NXT 1 ( p 15 ) is a crucial cellular cofactor in TAP dependent export of intron containing RNA in mammalian cells . ^^^ Significantly , cotransfection with a vector expressing NXT 1 ( p 15 ) , an NTF 2 related cellular factor that binds to TAP , led to dramatic enhancement of the ability of the chimeric proteins to mediate RNA export . ^^^ Mutant protein analysis demonstrated that the domain necessary for nuclear export mapped to the C terminal region of TAP and required the domain that interacts with NXT 1 , as well as the region that has been shown to interact with nucleoporins . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| RNA export mediated by tap involves NXT 1 dependent interactions with the nuclear pore complex . ^^^ Here , we have characterized the function of NXT 1 in the context of the Tap dependent RNA export pathway . ^^^ We demonstrate that NXT 1 stimulates binding of a Tap RNA complex to nucleoporins in vitro , and we provide mutational analysis that shows these interactions are necessary for nuclear export of an intron containing viral mRNA in vivo . ^^^ Tap contains separate domains for binding to nucleoporins and NXT 1 , both of which are critical for its export function . ^^^ RNA export is mediated by a heterodimer of Tap and NXT 1 , and the function of NXT 1 on this pathway is to regulate the affinity of the Tap RNA complex for nucleoporins within the nuclear pore complex . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| In the absence of NXT 1 binding , the Tap protein is unable to effectively interact with components of the nuclear pore complex and both Tap nucleocytoplasmic shuttling and the nuclear export of mRNA molecules tethered to Tap are therefore severely attenuated . ^^^ These data suggest that NXT 1 may act as a molecular switch that regulates the ability of Tap to mediate nuclear mRNA export by controlling the interaction of Tap with components of the nuclear pore . . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| Exogeneous expression of Tap and NXT 1 was necessary and sufficient to rescue Gag augmentation in 293 cells . ^^^ Overexpression experiments established that CTE , but not RU 5 , confers the responsiveness to Tap and NXT 1 and that CTE in conjunction with Tap and NXT 1 conferred a 30 fold increase in translational utilization of the cytoplasmic RNA . ^^^ Our results uncovered a previously unidentified role of CTE in conjunction with Tap and NXT 1 in commitment to efficient cytoplasmic RNA utilization . . ^^^ |
|
| Interacting proteins: Q9UKK6 and Q59E96 |
Pubmed |
SVM Score :0.0 |
| The Tap RNA complex was shown to bind to three nucleoporins , Nup 98 , p 62 , and RanBP 2 , and these interactions were enhanced by Nxt 1 . ^^^ Mutations in the Tap UBA region abolished interactions with all three nucleoporins , whereas the effect of point mutations within the NTF 2 like domain of Tap known to disrupt Nxt 1 binding or nucleoporin binding were nucleoporin dependent . ^^^ |
|