| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| It is composed of two subunits , HIF 1alpha and ARNT . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimer composed of HIF 1alpha and HIF 1beta subunits . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimer composed of HIF 1alpha and HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is , in fact , a heterodimer of two subunits , HIF 1alpha and HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is composed of an HIF 1alpha subunit and an HIF 1beta subunit . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is composed of 2 sub units , namely HIF 1alpha and HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.6347731 |
| In addition , HIF 1alpha acquired a new conformational state upon dimerization with Arnt , rendering HIF 1alpha more resistant to proteolytic digestion in vitro . 0.6347731^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.59243795 |
| Thus , we might suggest that hypoxia inhibits TCDD induced Cyp1a1 expression due to the competition between HIF 1alpha and the AhR for the Arnt in Hepa 1 cells . . 0.59243795^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.55076772 |
| It blocked the nuclear translocation of ARNT but not that of endogenous HIF 1alpha , and was associated with ARNT in the cytosol . 0.55076772^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.55115767 |
| ARNT interacting protein ( AINT ) , a protein expressed during embryonic development in the mouse , binds through its coiled coil region to the aryl hydrocarbon nuclear translocator protein ( ARNT ) and has a central portion that contains seven of the 24 amino acid repeats found in mERIC 1 . 0.55115767^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.5355321 |
| These data indicate that post translational modification of HIF 1alpha is not essential for its interaction with ARNT and DNA , and provide an in vitro system for the characterization of the molecular properties of HIF 1alpha . . 0.5355321^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Here we show that both 2 aminopurine and sodium fluoride , inhibitors of serine / threonine kinases and phosphatases , respectively , interfered with the hypoxic induction of HIF 1 DNA binding activity and expression of HIF 1 alpha and HIF 1 beta ( ARNT ) subunits . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 beta is a series of ARNT gene products , which can thus heterodimerize with either HIF 1 alpha or AHR . ^^^ HIF 1 alpha and HIF 1 beta ( ARNT ) RNA and protein levels were induced in cells exposed to 1 % O 2 and decayed rapidly upon return of the cells to 20 % O 2 , consistent with the role of HIF 1 as a mediator of transcriptional responses to hypoxia . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Finally , sequence comparison of the currently known bHLH / PAS proteins indicates a division into two phylogenetic groups : the Arnt group , containing Arnt , Arnt 2 , and Per , and the AhR group , consisting of AhR , Sim , and Hif 1alpha . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , a DNA binding complex implicated in the regulation of gene expression by oxygen , has been shown to consist of a heterodimer of two basic helix loop helix Per AHR ARNT Sim ( PAS ) proteins , HIF 1alpha , and HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The HIF 1alpha and HIF 1beta ( ARNT ; aryl hydrocarbon receptor nuclear translocator ) subunits were coimmunoprecipitated from nuclear extracts , indicating that these proteins heterodimerize in the absence of DNA . ^^^ Compared to 826 amino acid , full length HIF 1alpha , amino acids 1 166 mediated heterodimerization with HIF 1beta ( ARNT ) , but amino acids 1 390 were required for optimal DNA binding . ^^^ In cotransfection assays , forced expression of recombinant HIF 1alpha and HIF 1beta ( ARNT ) activated transcription of reporter genes containing EPO enhancer sequences with intact , but not mutant , HIF 1 binding sites . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| To identify new dimerization partners for the aromatic hydrocarbon receptor nuclear translocator ( Arnt ) , we used its N terminal region ( amino acids 1 470 ) as a target in a two hybrid screening procedure , and we cloned the murine form of hypoxia inducible factor 1alpha ( HIF1alpha ) . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We demonstrate expression of HIF 1 alpha and HIF 1 beta ( ARNT ) mRNA in all human , rat , and mouse organs assayed and show for the first time that HIF 1 mRNA expression was induced in brain , kidney , and lung when rats or mice were exposed to reduced ambient O 2 concentrations for 30 to 60 min . ^^^ The ubiquitous in vivo expression of HIF 1 alpha and HIF 1 beta ( ARNT ) mRNA is consistent with the proposed role of HIF 1 in coordinating adaptive transcriptional responses to hypoxia . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| When reporters containing VEGF sequences , in the context of the native VEGF or heterologous simian virus 40 promoter , were cotransfected with expression vectors encoding HIF 1alpha and HIF 1beta ( ARNT [ aryl hydrocarbon receptor nuclear translocator ] ) , reporter gene transcription was much greater in both hypoxic and nonhypoxic cells than in cells transfected with the reporter alone . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimer consisting of HIF 1alpha , which is encoded by the HIF1A gene , complexed with HIF 1beta , which is encoded by the ARNT gene . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that HIF 1 alpha required Arnt for DNA binding in vitro and functional activity in vivo . ^^^ Both the bHLH and PAS motifs of Arnt were critical for dimerization with HIF 1 alpha . ^^^ Strikingly , HIF 1 alpha exhibited very high affinity for Arnt in coimmunoprecipitation assays in vitro , resulting in competition with the ligand activated dioxin receptor for recruitment of Arnt . ^^^ Both HIF 1 alpha and Arnt mRNAs were expressed constitutively in a large number of human tissues and cell lines , and these steady state expression levels were not affected by exposure to hypoxia . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| This protein complex is composed of two subunits , HIF 1alpha and 1beta ( aryl hydrocarbon receptor nuclear translocator , ARNT ) . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The HIF 1 alpha subunit is unique to HIF 1 , whereas HIF 1 beta ( ARNT ) can dimerize with other bHLH PAS proteins . ^^^ Structural analysis of HIF 1 alpha revealed that dimerization with HIF 1 beta ( ARNT ) requires the HLH and PAS domains , DNA binding is mediated by the basic domain , and that HIF 1 alpha contains a carboxyl terminal transactivation domain . ^^^ Co transfection of HIF 1 alpha and HIF 1 beta ( ARNT ) expression vectors and a reporter gene containing a wild type hypoxia response element resulted in increased transcription in non hypoxic cells and a superinduction of transcription in hypoxic cells , whereas HIF 1 expression vectors had no effect on the transcription of reporter genes containing a mutation in the HIF 1 binding site . ^^^ HIF 1 alpha and HIF 1 beta ( ARNT ) protein levels were induced by hypoxia in all primary and transformed cell lines examined . ^^^ HIF 1 alpha and HIF 1 beta ( ARNT ) mRNAs were detected in all human , mouse , and rat organs assayed and mRNA expression was modestly induced in rodents subjected to hypoxia . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The discovery that the oxygen regulated transcription factor HIF 1 alpha and the dioxin receptor AhR share the common heterodimerization partner ARNT ( HIF 1 beta ) raised the question whether a cross talk between oxygen and dioxin signal transduction pathways exists . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation studies , yeast two hybrid analysis , and transient transfection experiments demonstrated that MOP 1 and MOP 2 dimerize with ARNT and that these complexes are transcriptionally active at defined DNA enhancer sequences in vivo . ^^^ The expression profiles of the AHR , MOP 1 , and MOP 2 mRNAs , coupled with the observation that they all share ARNT as a common dimeric partner , suggests that the cellular pathways mediated by MOP 1 and MOP 2 may influence or respond to the dioxin signaling pathway . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We have isolated and characterized a cDNA for a novel Per Arnt / AhR Sim basic helix loop helix ( bHLH PAS ) factor that interacts with the Ah receptor nuclear translocator ( Arnt ) , and its predicted amino acid sequence exhibits significant similarity to the hypoxia inducible factor 1alpha ( HIF1alpha ) and Drosophila trachealess ( dTrh ) gene product . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The arylhydrocarbon receptor nuclear translocator ( ARNT ) is a member of the basic helix loop helix PAS family of heterodimeric transcription factors which includes the arylhydrocarbon receptor ( AHR ) , hypoxia inducible factor 1alpha ( HIF 1alpha ) and the Drosophila single minded protein ( Sim ) . ^^^ ARNT forms heterodimeric complexes with the arylhydrocarbon receptor , HIF 1alpha , Sim and the PAS protein Per . ^^^ In response to environmental pollutants , AHR ARNT heterodimers regulate genes involved in the metabolism of xenobiotics , whereas ARNT HIF 1alpha heterodimers probably regulate those involved in the response to oxygen deprivation . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Transient co expression of the two HIF 1 subunits HIF 1alpha and aryl hydrocarbon receptor nuclear translocator ( ARNT ) / HIF 1beta resulted in enhanced reporter gene expression even under normoxic conditions . ^^^ DNA binding studies using nuclear extracts from the mouse hepatoma cell line Hepa 1 and the ARNT / HIF 1beta deficient subline Hepa1C4 , as well as antibodies raised against HIF 1alpha and ARNT / HIF 1beta confirmed that HIF 1 binds the Tf HBSs . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We also demonstrated that mSIM 2 can functionally interfere with hypoxia inducible factor 1alpha ( HIF 1alpha ) / ARNT transcription complexes , providing a second mechanism by which mSIM 2 may inhibit transcription . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The HIF 1 heterodimer is composed of a HIF 1alpha and an ARNT subunit , both belonging to the explosively growing PAS subfamily of bHLH transcription factors . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Upon exposure to the above conditions , HIF 1 alpha is upregulated and heterodimerizes with the Ah receptor nuclear translocator ( ARNT , also known as HIF 1 beta ) , the heterodimeric complex binds TACGTG containing genomic enhancer elements , and activates transcription of target genes . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Considering these results and that Arnt or Arnt 2 functions as a common partner in the formation of transcriptional regulators with other bHLH / PAS proteins such as AhR , HLF , and HIF 1alpha , the possibility arises that CBP / p300 is extensively involved as a coactivator in the transactivation process by bHLH / PAS ( a conserved sequence motif among Per , Arnt , and Sim ) heterodimer transcription factors through interaction with Arnt or Arnt2 . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Expression of ARNT , ARNT 2 , HIF 1 alpha , HIF 2 alpha and Ah receptor mRNAs in the developing mouse . ^^^ The basic helix loop helix PAS ( bHLH PAS ) protein ARNT is a dimeric partner of the Ah receptor ( AHR ) and hypoxia inducible factor 1 alpha ( HIF 1 alpha ) . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The transcriptional response to lowered oxygen levels is mediated by the hypoxia inducible transcription factor ( HIF 1 ) , a heterodimer consisting of the constitutively expressed aryl hydrocarbon receptor nuclear translocator ( ARNT ) and the hypoxic response factor HIF 1alpha . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The partner PAS proteins of Arnt 3 were searched for by the two hybrid system in yeast , and HIF 1 alpha , HLF , and Clock among various bHLH / PAS proteins were found . ^^^ Gel mobility shift analysis using nuclear extracts from 293T cells cotransfected with Arnt 3 and HIF 1 alpha ( or HLF ) expression plasmids revealed that these complexes specifically bound the hypoxia response element ( HRE ) . ^^^ Coexpression of Arnt 3 and HIF 1 alpha ( or HLF ) in Arnt deficient c 4 cells enhanced transcription of a reporter gene driven by the HRE sequences . ^^^ Transcriptionally active heterodimer formation of an Arnt like PAS protein , Arnt 3 , with HIF 1a , HLF , and clock . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Insulin induces transcription of target genes through the hypoxia inducible factor HIF 1alpha / ARNT . ^^^ Induction of these genes is mediated by a common basic helix loop helix PAS transcription complex , the hypoxia inducible factor 1alpha ( HIF 1alpha ) / aryl hydrocarbon nuclear translocator ( ARNT ) . ^^^ We report here that insulin shares with hypoxia the ability to induce the HIF 1alpha / ARNT transcription complex in various cell types . ^^^ This induction was demonstrated by electrophoretic mobility shift of the hypoxia response element ( HRE ) , and abolished by specific antisera to HIF 1alpha and ARNT , and by transcription activation of HRE reporter vectors . ^^^ Finally , insulin like growth factor 1 ( IGF 1 ) also induced the HIF 1alpha / ARNT transcription complex . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| An important mediator is hypoxia inducible factor 1 ( HIF 1 ) , a DNA binding complex shown to contain at least two basic helix loop helix PAS domain ( bHLH PAS ) proteins , HIF 1alpha and aryl hydrocarbon nuclear receptor translocator ( ARNT ) . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In response to decreased cellular oxygen concentrations the basic helix loop helix ( bHLH ) / PAS ( Per , Arnt , Sim ) hypoxia inducible transcription factor , HIF 1alpha , mediates activation of networks of target genes involved in angiogenesis , erythropoiesis and glycolysis . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1alpha ( hypoxia inducible factor 1alpha ) is a basic helix loop helix PAS ( Per / Arnt / Sim ) transcription factor that , under hypoxic conditions , dimerizes with a partner factor , the basic helix loop helix / PAS protein Arnt , to recognize hypoxia responsive elements of target genes . ^^^ Here we show that inhibitors of 26 S proteasome activity produced a dramatic accumulation of endogenous as well as transfected HIF 1alpha protein under normoxic conditions , whereas the levels of Arnt protein were not affected . ^^^ HIF 1alpha ( hypoxia inducible factor 1alpha ) is a basic helix loop helix PAS ( Per / Arnt / Sim ) transcription factor that , under hypoxic conditions , dimerizes with a partner factor , the basic helix loop helix / PAS protein Arnt , to recognize hypoxia responsive elements of target genes . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Induction and nuclear translocation of hypoxia inducible factor 1 ( HIF 1 ) : heterodimerization with ARNT is not necessary for nuclear accumulation of HIF 1alpha . ^^^ HIF 1 consists of two subunits , HIF 1alpha and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) . ^^^ Whereas hypoxia prevents proteasomal degradation of HIF 1alpha , ARNT expression is thought to be oxygen independent . ^^^ Here , we have used ARNT mutant mouse hepatoma and embryonic stem cells to examine the requirement of ARNT for accumulation and nuclear translocation of HIF 1alpha in hypoxia . ^^^ As shown by immunofluorescence , HIF 1alpha accumulation in the nucleus of hypoxic cells was independent of the presence of ARNT , suggesting that nuclear translocation is intrinsic to HIF 1alpha . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In hypoxic condition , Arnt dimerizes with HIF 1alpha to make HIF 1alpha / Arnt heterodimer which is able to bind hypoxia responsive DNA elements . ^^^ The HIF 1alpha / Arnt heterodimer functions as a transactivator for hypoxia inducible genes . ^^^ Given that the expression of Arnt is limited , HIF 1alpha may compete with AhR for recruiting Arnt as a heteromeric partner . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a transcription factor composed of two subunits , HIF 1alpha and ARNT , which is activated under hypoxia . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 , which is a heterodimeric complex of HIF 1 alpha and aryl hydrocarbon receptor nuclear translecator ( ARNT ) , binds to HIF 1 element . ^^^ Mammalian two hybrid experiments in vivo revealed that transitional change took place from the interaction of HNF 4 with ARNT to that with HIF 1 alpha in response to hypoxia . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The aryl hydrocarbon nuclear translocator ( Arnt ) is a basic helix loop helix ( per / Arnt / Ahr / sim ) PAS containing protein that can heterodimerize with the aryl hydrocarbon receptor ( AhR ) , the hypoxia inducible factor 1 alpha , and other PAS containing proteins to form transcriptionally active complexes . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| ARNT is the central partner of several heterodimeric transcription factors , including those containing the aryl hydrocarbon ( dioxin ) receptor ( AhR ) and the hypoxia inducible factor 1alpha ( HIF1alpha ) . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Finally , the negative regulatory function of this factor appears to be restricted for dioxin signaling since Arnt was able to mediate , together with hypoxia inducible factor 1alpha , transcriptional activation in hypoxic cells . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| ARNT is not necessary for hypoxic induction of HIF 1 alpha in the nucleus . ^^^ HIF 1 consists of two subunits , HIF 1 alpha and the aryl hydrocarbon receptor nuclear translocator ( ARNT ) . ^^^ Whereas hypoxia prevents ubiquitination and proteasomal degradation of HIF 1 alpha , ARNT expression is thought to be oxygen independent . ^^^ To examine the requirement of ARNT for accumulation and nuclear translocation of HIF 1 alpha in hypoxia , we used ARNT mutant mouse hepatoma and ARNT deficient embryonic stem cells . ^^^ As shown by immunofluorescence , HIF 1 alpha accumulation in the nucleus of hypoxic cells did not require ARNT , demonstrating that nuclear translocation is intrinsic to HIF 1 alpha . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The HIF 1 complex is composed of the ubiquitously expressed basic helix loop helix / PAS ( bHLH / PAS ) proteins HIF 1alpha and Arylhydrocarbon Receptor Nuclear Translocator ( ARNT ) . ^^^ ARNT 2 is a conserved ARNT homolog that is highly expressed in neurons , suggesting that ARNT2 / HIF 1alpha heterodimers mediate transcriptional responses to oxygen deprivation in the nervous system . ^^^ Formation of neural ARNT2 / HIF 1alpha complexes in Arnt ( / ) ES cell derived teratocarcinomas may explain why these tumors express VEGF , vascularize and grow efficiently , in contrast to ARNT deficient hepatomas . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The heterodimeric hypoxia inducible factor 1 ( HIF 1 ) , consisting of the subunits HIF 1alpha and HIF 1beta / ARNT , is a master transcriptional regulator of oxygen homeostasis . ^^^ Direct assessment of translational regulation by polysomal profile analysis of HeLaS 3 cells showed that HIF 1alpha ( and to a lower extent ARNT ) mRNA was found mainly in the translationally active polyribosomal fractions under both normoxic and hypoxic conditions . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Isolation and characterization of AINT : a novel ARNT interacting protein expressed during murine embryonic development . ^^^ Herein , we describe a novel , embryonically expressed , ARNT interacting protein ( AINT ) that may be a member of a larger coiled coil PAS interacting protein family . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Cobalt chloride induction of the glucose transporter 1 and heme oxygenase 1 mRNAs was also markedly abrogated in mice lacking ARNT expression , suggesting an inhibition of HIF 1alpha activity . ^^^ These studies establish a critical role for ARNT in AHR and HIF 1alpha signal transduction in the intact mouse . . ^^^ Conditional disruption of the aryl hydrocarbon receptor nuclear translocator ( Arnt ) gene leads to loss of target gene induction by the aryl hydrocarbon receptor and hypoxia inducible factor 1alpha . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| It is composed of two different subunits , HIF 1alpha and ARNT ( Aryl Receptor Nuclear Translocator ) . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , consisting of HIF 1alpha and ARNT subunits , activates many genes involved in the cellular and organismal response to O ( 2 ) deprivation . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Under hypoxia , HIF 1alpha binds to aryl hydrocarbon receptor nuclear translocator ( ARNT , also called HIF 1beta ) to activate expression of genes important for cell survival . ^^^ The phosphorylated HIF 1alpha was the major form that bound to ARNT . ^^^ Ectopically expressed ARNT was consistently able to enhance HIF 1alpha phosphorylation in a binding dependent manner . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HNF 4 was observed to interact with HIF 1alpha and HIF 1beta ( arylhydrocarbon receptor nuclear translocator , ARNT ) during hypoxia . ^^^ In addition , the TKQE motif of HNF 4 was essential for protein protein interactions with HIF 1alpha and ARNT . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| On the other hand , HIF 1alpha dimerized with ARNT , as an active transcription factor , can protect cells from apoptosis induced by several conditions . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| ARNT is an indispensable partner of the aryl hydrocarbon receptor ( AHR ) or hypoxia inducible factor 1alpha . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Transcriptional activation of hypoxia regulated genes is mediated by hypoxia inducible factor 1 ( HIF 1 ) , a heterodimer of HIF 1alpha and ARNT ( Ah receptor nuclear translocator ; HIF 1beta ) . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia induced gene expression depends primarily on stabilization of the alpha subunit of hypoxia inducible factor 1 ( HIF 1alpha ) , which acts as a heterodimeric trans activator with the nuclear protein known as the aryl hydrocarbon receptor nuclear translocator ( Arnt ) . ^^^ The resulting heterodimer ( HIF 1alpha / Arnt ) interacts specifically with the hypoxia responsive element ( HRE ) , thereby increasing transcription of the genes under HRE control . ^^^ However , this dose of radicicol significantly reduces HRE binding by the HIF 1alpha / Arnt heterodimer . ^^^ Our results , the first to show that radicicol specifically inhibits the interaction between the HIF 1alpha / Arnt heterodimer and HRE , suggest that Hsp 90 modulates the conformation of the HIF 1alpha / Arnt heterodimer , making it suitable for interaction with HRE . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| This transcription factor is composed of two subunits : HIF 1alpha and ARNT ( aryl hydrocarbon receptor nuclear translocator ) . ^^^ ARNT is constitutively expressed , whereas HIF 1alpha is targeted to proteasome degradation by ubiquitination during normoxia . ^^^ In hypoxia , HIF 1alpha is stabilized and translocates to the nucleus , where it binds to ARNT . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The correlation of HIF 1alpha and P gp expression was validated by the use of hepatoma tumor spheroids that were either wild type ( Hepa 1 ) or mutant ( Hepa1C4 ) for aryl hydrocarbon receptor nuclear translocator ( ARNT ) , i . e . , HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The aryl hydrocarbon receptor ( AhR ) is a member of the basic helix loop helix PAS ( Per ARNT SIM ) transcription family , which also includes hypoxiainducible factor 1alpha ( HIF 1alpha ) and its common dimerization partner AhR nuclear translocator ( ARNT ) . ^^^ Following ligand activation or hypoxia , AhR or HIF 1alpha , respectively , translocate into the nucleus , dimerize with ARNT , and regulate gene expression . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| As a hypoxia inducible transcription factor HIF 1 is a heterodimer composed of the helix loop helix Per Arnt Sim ( bHLH PAS ) proteins HIF 1alpha and the aryl hydrocarbon nuclear translocator ( ARNT ) also known as HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Here we show that ARNT ( aryl hydrocarbon receptor nuclear translocator ) , the obligatory heterodimerization partner for the aryl hydrocarbon receptor and hypoxia inducible factor 1alpha , functions as a potent coactivator of ERalpha and ERbeta dependent transcription . ^^^ These observations , together with the previous role of ARNT as an obligatory partner protein for conditionally regulated basic helix loop helix PAS proteins like the aryl hydrocarbon receptor or hypoxia inducible factor 1alpha , expand the cellular functions of ARNT to include regulation of ERalpha and ERbeta transcriptional activity . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia induced gene expression is mediated by the hypoxia inducible factor 1 ( HIF 1 ) that consists of HIF 1alpha and the aromatic hydrocarbon nuclear translocator ( Arnt ) . ^^^ Our results indicate that hypoxia but not insulin stabilizes HIF 1alpha protein levels , whereas both insulin and hypoxia induced gene expression require the presence of the Arnt protein . ^^^ Although both hypoxia and insulin induce the expression of common target genes through a hypoxia responsive element and Arnt dependent mechanism , insulin can not stabilize the HIF 1alpha protein . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The arylhydrocarbon receptor nuclear translocator ( ARNT ) is a member of the basic helix loop helix , PER ARNT SIM family of heterodimeric transcription factors , and serves as a dimerization partner for arylhydrocarbon receptor ( AHR ) and hypoxia inducible factor 1alpha . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| PAS regions of Arnt , DR , and HIF 1alpha and evaluated the contribution of the PAS domains to DNA binding in vitro . ^^^ PAS regions of DR or HIF 1alpha with the corresponding region of Arnt . ^^^ Formation of stable protein DNA complexes by DR / Arnt and HIF 1alpha / Arnt heterodimers with their cognate DNA sequences required the PAS A domains and exhibited KD values of 0 . 4 nM and approximately 50 nM , respectively . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| While HSP 90 and HSP 70 bound to HIF 1alpha predominantly under normoxic conditions , ARNT bound to HIF 1alpha primarily under hypoxic conditions , suggesting that ARNT displaced HSP 90 from HIF 1alpha following nuclear translocation . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In the present study , we identified a novel ARNT partner , a HIF 1alpha variant , which is ubiquitously expressed in human tissues and cancer cell lines . ^^^ The HIF 1alpha variant , designated HIF 1alpha417 , bound to ARNT and , moreover , stimulated the transcription of the erythropoietin enhancer reporter gene . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We initially identified TRIP 230 as an ARNT interacting protein in a yeast two hybrid assay screen . ^^^ Similarly , tissue responses to hypoxia are largely mediated through the HIF 1 heterodimeric transcription factor , comprising hypoxia inducible factor 1 alpha ( HIF 1 alpha ) and ARNT . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1alpha ( HIF 1alpha ) and aryl hydrocarbon receptor ( AhR ) both require dimerization with AhR nuclear translocator ( ARNT ) to initiate transcription of their respective target genes . ^^^ Hypoxia inducible factor 1alpha ( HIF 1alpha ) and aryl hydrocarbon receptor ( AhR ) both require dimerization with AhR nuclear translocator ( ARNT ) to initiate transcription of their respective target genes . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We also observed that CBP , HIF 1alpha / Arnt and HIF 1alpha / CBP accumulation foci partially overlap with the hyperphosphorylated form of RNA polymerase 2 , and that CBP had a stabilizing effect on the formation of the complex between HIF 1alpha and its DNA binding partner , Arnt . ^^^ In conclusion , CBP plays an important role as a mediator of HIF 1alpha / Arnt / CBP / SRC 1 complex formation , coordinating the temporally and hierarchically regulated intranuclear traffic of HIF 1alpha and associated cofactors in signal transduction in hypoxic cells . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In order to examine the role of HIF in von Hippel Lindau ( VHL ) associated vascular tumorigenesis , we utilized Cre loxP mediated recombination to inactivate hypoxia inducible factor 1alpha ( Hif 1alpha ) and arylhydrocarbon receptor nuclear translocator ( Arnt ) genes in a VHL mouse model of cavernous liver hemangiomas and polycythemia . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Under low oxygen tension , the activated transcription factor HIF 1alpha upregulates an array of hypoxia inducible genes via heterodimerization with ARNT and binding to the hypoxia responsive element in the promoter . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is composed of the two subunits HIF 1alpha and HIF 1beta ( also called ARNT ) . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| When this collection of 50 , 000 siRNAs was used to identify genes involved in the cellular response to hypoxia , two of the most potent hits were the key hypoxia transcription factors HIF1A and ARNT . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| A novel Arnt interacting protein Ainp 2 enhances the aryl hydrocarbon receptor signalling . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Inactivation of the HIF alpha binding partner , arylhydrocarbon receptor nuclear translocator ( Arnt ) , but not Hif 1alpha , suppressed the development of renal cysts . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Expression of DmagARNT in ARNT lacking mouse Hepa c 4 cells resulted in the compensation for the loss of hypoxia response , suggesting the formation of a dimer with mouse HIF 1alpha and that the resulting heterodimer binds to the hypoxia responsive elements ( HRE ) , leading to transcription of the downstream luciferase gene . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In order to develop a simple and genetically tractable in vivo system for HIF 1 analysis , we tested the inducible expression of both human HIF 1 subunits ( HIF 1alpha and ARNT ) in the yeast Saccharomyces cerevisiae and showed the formation of transcriptionally active HIF 1 . ^^^ Furthermore , using yeast strains co expressing truncated forms of HIF 1alpha with ARNT or both HIF 1alpha and ARNT , we characterized fragments of HIF 1alpha that acted as dominant negative mutants and suppressed HIF 1 activity . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The hypoxia inducible transcription factor HIF 1 is a heterodimer composed of the helix loop helix Per Arnt Sim ( bHLH PAS ) proteins HIF 1alpha and the aryl hydrocarbon nuclear translocator ( ARNT ) also known as HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The aryl hydrocarbon receptor nuclear translocator ( Arnt ) , also called Hif 1beta , serves as an obligate heterodimerization partner of Hif 1alpha and Hif 2alpha . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Both HIF 1alpha and HIF 1beta mRNAs were constitutively expressed in HeLa and Hep3B cells with no significant induction by hypoxia . ^^^ However , the HIF 1alpha protein was barely detectable in normoxic cells , even when HIF 1alpha was overexpressed , but was highly induced in hypoxic cells , whereas HIF 1beta protein levels remained constant , regardless of pO 2 . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| A role for HIF 1 in HO 1 gene regulation was established by the following observations : 1 ) HIF 1 specifically bound to an oligonucleotide spanning these sequences , 2 ) mutation of these sequences abolished HIF 1 binding and hypoxia dependent gene activation in VSM cells , 3 ) hypoxia increased HIF 1alpha and HIF 1beta protein levels in VSM cells , and 4 ) hypoxia dependent HO 1 mRNA accumulation was not observed in mutant hepatoma cells lacking HIF 1 DNA binding activity . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Sequences from HIF 1alpha but not HIF 1beta conferred oxygen regulated activity . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Whereas HIF 1alpha is a novel member of this family , HIF 1beta is identical to the aryl hydrocarbon receptor nuclear translocator , previously recognized to be involved in xenobiotic metabolism . cDNA cloning revealed that mouse HIF 1alpha can be expressed as two mRNA isoforms containing alternative 5 ' untranslated regions and two different predicted translational start sites . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a basic helix loop helix transcription factor composed of HIF 1alpha and HIF 1beta subunits . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Under hypoxic conditions Hep 3B cells transfected with the HIF 1alpha cDNA alone showed a 2 . 2 fold increase in luciferase activity , HIF 1beta showed a 3 . 4 fold increase and cells transfected with HIF 1 alpha and beta showed a 6 . 9 fold increase in activity over cells transfected with pREP 4 . ^^^ Cells were co transfected with the 38 bp Epo enhancer fragment bearing the HIF 1 binding motif , subcloned in the luciferase reporter plasmid and either the HIF 1alpha cDNA , HIF 1beta cDNA , HIF 1alpha and HIF 1beta cDNAs or pREP 4 respectively . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 itself consists of a heterodimer of two basic helix loop helix proteins of the PAS family , termed HIF 1alpha and HIF 1beta , although other closely related members of this family may also contribute to the response to hypoxia . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Induction of p 53 does not occur in either a mutant hepatoma cell line that is unable to induce HIF 1alpha or embryonic stem cells derived from mice lacking HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The hypoxia inducible factor 1 ( HIF 1 ) , a transcription factor consisting of the two proteins HIF 1alpha and HIF 1beta , plays a major role in the pleiotropic response observed under low oxygen . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : Hypoxia inducible factor 1 ( HIF 1 ) is a transcription factor composed of HIF 1alpha and HIF 1beta subunits . ^^^ Eyes were frozen , and retinas were isolated and used for immunoblot analysis , or eyes were sectioned for immunohisto chemical staining for HIF 1alpha or HIF 1beta , or for in situ hybridization for VEGF . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a dimeric transcription factor composed of HIF 1alpha and HIF 1beta subunits that plays an essential role in mammalian O 2 homeostasis . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The HIF 1 complex is composed of two b HLH proteins : HIF 1beta that is constitutively expressed , and HIF 1alpha , that is present only in hypoxic cells . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The expression of VEGF and HIF 1 subunits ( HIF 1alpha and HIF 1beta ) was enhanced by treatment with picroliv during normoxia and hypoxia in HUVEC and Hep 3B cells and on reoxygenation the expression of these genes was significantly reduced as revealed by mRNA analysis using RT PCR . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Transient co expression of the two HIF 1 subunits ( HIF 1alpha and HIF 1beta ) enhanced the wild type transferrin receptor promoter activity , but that which contained a mutated HBS yielded no such response . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We used Northern blot analysis to show an increase in the levels of AM and HIF 1alpha mRNA but not HIF 1beta mRNA in the HL 1 cardiac myocyte cell line in response to hypoxia . ^^^ Furthermore , Western blot analysis revealed that the levels of both HIF 1alpha and HIF 1beta protein increased under hypoxic conditions . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimeric transcription factor , induced by hypoxia , that is composed of HIF 1alpha and HIF 1beta protein subunits . ^^^ Because HIF 1 activation might promote cell survival in hypoxic tissues , we studied the effect of permanent middle cerebral artery occlusion on the expression of HIF 1alpha , HIF 1beta and several HIF 1 target genes in adult rat brain . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The involvement of hypoxia inducible factor 1 ( HIF 1 ) was shown by gel shift and supershift experiments that showed HIF 1alpha and HIF 1beta binding to a radiolabeled oligonucleotide containing the Cp promoter HRE . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| This AM expression seems to be under hypoxia inducible factor 1 ( HIF 1 ) transcriptional regulation , since HIF 1alpha and HIF 1beta knockout mouse cell lines had an ablated or greatly reduced hypoxia AM mRNA induction . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| METHODS : The authors investigated the expression of the HIF 1alpha and HIF 1beta subunits in human glioma cell lines and brain tumor tissues using Western blot analysis and immunohistochemistry . ^^^ Like HIF 1alpha , HIF 1beta was most highly expressed in high grade tumors but was expressed more widely than HIF 1alpha , including cells away from necrotic zones . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Using the supershift assay we have shown that this hypoxia responsive element binds HIF 1alpha and HIF 1beta proteins . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a heterodimer composed of HIF 1alpha and HIF 1beta protein subunits . ^^^ Hypoxic preconditioning ( 8 % O 2 for 3 hours ) , a treatment known to protect the newborn rat brain against hypoxic ischemic injury , markedly increased HIF 1alpha and HIF 1beta expression . ^^^ HIF 1alpha and HIF 1beta protein levels were markedly increased after intraperitoneal injection of CoCl 2 ( 60 mg / kg ) and moderately increased after intraperitoneal injection of DFX ( 200 mg / kg ) 1 to 3 hours after the injections . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 consists of two proteins , HIF 1alpha and HIF 1beta . ^^^ HIF 1alpha accumulates under hypoxic conditions , whereas HIF 1beta is constitutively expressed . ^^^ HIF 1alpha and HIF 1beta expression were measured during adaptation to hypobaric hypoxia ( 0 . 5 atm ) in rat cerebral cortex . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Induction of HIF 1 binding activity was consistent with an increased expression of HIF 1 subunit proteins HIF 1alpha and HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Exercise induced expression of angiogenesis related transcription and growth factors in human skeletal muscle . mRNA expression of vascular endothelial growth factor ( VEGF ) , fibroblast growth factor 2 ( FGF 2 ) , and hypoxia inducible factor ( HIF ) subunits HIF 1alpha and HIF 1beta in human skeletal muscle was studied during endurance exercise at different degrees of oxygen delivery . ^^^ Exercise increased VEGF mRNA expression by 178 % and HIF 1beta by 340 % , but not HIF 1alpha and FGF 2 . ^^^ The exercise induced increase in VEGF mRNA was correlated to the exercise changes in HIF 1alpha and HIF 1beta mRNA . ^^^ The changes in VEGF , HIF 1alpha , and HIF 1beta mRNAs were correlated to the exercise induced increase in femoral venous plasma lactate concentration . ^^^ It is concluded that 1 ) VEGF but not FGF 2 gene expression is upregulated in human skeletal muscle by a single bout of dynamic exercise and that there is a graded response in VEGF mRNA expression related to the metabolic stress and 2 ) the increase in VEGF mRNA expression correlates to the changes in both HIF 1alpha and HIF 1beta mRNA . . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimer consisting of a constitutively expressed HIF 1beta subunit and an O 2 and growth factor regulated HIF 1alpha subunit . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Introduction of wild type PTEN into the PTEN negative PC 3 cell line specifically inhibited the expression of HIF 1alpha but not that of HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The HIF 1beta subunit is constitutively expressed , whereas the HIF 1alpha subunit is subject to ubiquitination and proteasomal degradation , a process that is inhibited under hypoxic conditions . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| On immunohistochemistry and in situ hybridization , endothelial cells in the plexiform lesions expressed VEGF mRNA and protein and overexpressed the mRNA and protein of VEGFR 2 , and the transcription factor subunits HIF 1alpha and HIF 1beta of hypoxia inducible factor , which are responsible for the hypoxia dependent induction of VEGF . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Whereas HIF 1beta is constitutively expressed in brain and kidney nuclear extracts , its hepatic expression increases concomitantly with HIF 1alpha . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Cells lacking either HIF 1alpha , or the second composite sub unit of HIF 1 , HIF 1beta , were markedly more sensitive to the combined stress of perturbed redox and hypoglycaemia than wild type cells . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| METHODS : HIF 1alpha , HIF 2alpha and HIF 1beta were immunolocalized in cycling endometrium . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is composed of an O 2 and growth factor regulated HIF 1alpha subunit and a constitutively expressed HIF 1beta subunit . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is composed of HIF 1alpha and HIF 1beta protein subunits . ^^^ Because HIF 1 activation might promote cell survival in hypoxic tissues , we studied the effect of stroke on the expression of HIF 1alpha , HIF 1beta and several HIF 1 target genes in adult rat brain . ^^^ Hypoxic preconditioning ( 8 % O2 / 3 hrs ) , a treatment known to protect the newborn rat brain against hypoxic ischemic injury , markedly increased HIF 1alpha and HIF 1beta expression . ^^^ HIF 1alpha and HIF 1beta protein levels were markedly increased after i . p . injection of CoCl 2 and DFX . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Tie 2 mRNA was detected in both MVEC types , but levels were 150 times as high in CK MVECs as in CK+ MVECs . mRNA of hypoxia inducible factors Hif 1alpha and Hif 1beta was expressed in both MVEC types . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| It consists of two subunits , HIF 1alpha , which is degraded during normoxia , and HIF 1beta , which is constitutively expressed . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , a transcription factor composed of HIF 1alpha and HIF 1beta protein subunits , has been implicated in cellular protection and cell death in cerebral ischemia . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , a heterodimeric transcription factor consisting of HIF 1alpha and HIF 1beta subunits , controls the expression of a large number of genes involved in the regulation of cellular responses to reduced oxygen availability . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , a heterodimer of HIF 1alpha and HIF 1beta subunits , is a transcriptional activator central to the cellular response to low oxygen that includes metabolic adaptation , angiogenesis , metastasis , and inhibited apoptosis . ^^^ Changes in HIF 1alpha mRNA levels did not account for the changes observed at the protein level , and HIF 1beta protein levels did not change . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The present study demonstrates that Cr ( 6 ) induces hypoxia inducible factor 1 ( HIF 1 ) activity through the specific expression of HIF 1alpha but not HIF 1beta subunit and increases the level of vascular endothelial growth factor ( VEGF ) expression in DU 145 human prostate carcinoma cells . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a heterodimeric HIF 1alpha / HIF 1beta complex that regulates the transcription of hypoxia responsive genes . ^^^ Exogenous HIF 1alpha markedly increased luciferase reporter activity driven by the leptin promoter when HIF 1beta was co expressed in the same cells . ^^^ These data suggest that HIF 1alpha / HIF 1beta dimers are involved in the effect of CoCl 2 to activate the leptin promoter . ^^^ To confirm the implication of HIF 1 , the cells were transfected with a dominant negative form of HIF 1alpha producing transcriptionally inactive HIF 1beta / HIF 1alpha dimers . ^^^ Electrophoretic mobility shift assays performed with in vitro translated HIF 1alpha and HIF 1beta proteins demonstrated binding to this HRE and not to mutated sequences only when both subunits were used together . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a heterodimeric transcription factor containing an inducibly expressed HIF 1alpha subunit and a constititutively expressed HIF 1beta subunit . ^^^ Under hypoxic conditions , the HIF 1alpha subunit accumulates due to a decrease in the rate of proteolytic degradation , and the resulting HIF 1alpha HIF 1beta heterodimers undergo posttranslational modifications that promote transactivation . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In these regions , we identified functional hypoxia response elements ( HREs ) that bound to HIF 1alpha and HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Differential techniques have revealed several novel genes and peptides involved in trophoblast development including PL74 / gdf15 / MIC 1 , a TGFbeta family cytokine that controls apoptosis and differentiation , PL 48 , a new serine threonine protein kinase , serum and glucocorticoid induced kinase , PBK 1 , a tunicamycin responsive gene , a cathepsin D like gene ( DAP 1 ) and hypoxia regulated genes HRF 1 , 2 , 6 , 8 and HIF 1alpha , HIF 1beta , and hEPAS 1 . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is composed of HIF 1alpha and HIF 1beta , and is a master regulator of oxygen homeostasis , playing critical roles in physiological and pathological processes . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is composed of HIF 1alpha ( subjected to protein stability regulation ) and constitutively expressed HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| It consists of a regulatory subunit HIF 1alpha , which accumulates under hypoxic conditions , and a constitutively expressed subunit HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Expression of HIF 1alpha and HIF 1beta messengers and HIF 1beta protein subunit remained unchanged regardless of O ( 2 ) tension , whereas HIF 1alpha protein subunit was overexpressed under hypoxic conditions . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 consists of a constitutively expressed HIF 1 beta ( HIF 1beta ) subunit and an oxygen regulated HIF 1 alpha ( HIF 1a ) subunit . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimer comprised of oxygen regulated HIF 1alpha and constitutively expressed HIF 1beta subunits . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In response to hypoxia , stabilized HIF 1alpha and HIF 2alpha proteins bind HIF 1beta and initiate expression of genes that alleviate hypoxic stress , including those promoting neovascularization . ^^^ Because VEGF is highly expressed by metanephric podocytes and collecting ducts , developing mouse kidney was examined for the presence and distribution of HIF 1alpha , HIF 2alpha , and HIF 1beta . ^^^ Both HIF 1 and HIF 2 heterodimers were identified in newborn kidney lysates by immunoprecipitation with HIF 1alpha , HIF 2alpha , and HIF 1beta antibodies and Western blots . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| According to current thinking , hypoxia is `` sensed ' ' by hydroxylases that permit Hypoxia Inducible Factor 1alpha ( HIF 1alpha ) to complex with HIF 1beta to form a transcriptional activator ( HIF 1 ) that drives expression of hypoxia sensitive genes ( such as EPO ) under hypoxic conditions . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| To test whether known HIF 1 and HIF 2 are involved in hypoxia induced heNOS promoter activation , HMEC 1 and HUVEC were transiently transfected with HIF 1alpha and HIF 1beta or HIF 2alpha and HIF 1beta expression vectors . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimeric transcription factor and is composed of two subunits , the oxygen sensitive HIF 1alpha and constitutively expressed HIF 1beta . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Although the degradation rate of VEGF mRNA under hypoxic conditions was similar in both cell lines , hypoxia inducible factor 1alpha ( HIF 1alpha ) mRNA , but not HIF 1beta mRNA , was found to be constitutively upregulated in A 11 cells compared to P 29 cells . ^^^ |
|
| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The present investigation provides for the first time , unambiguous information on the occurrence of hypoxia inducible factors ( HIF 1alpha and HIF 1beta proteins ) in normoxia ( Nx ) and their interaction with hypoxia ( Hx ) and intracellular Fe ( 2+ ) chelation in the rat carotid body ( CB ) glomus cells . ^^^ HIF 1alpha bound to HIF 1beta translocated into the nucleus is identified on the basis of immunohistochemistry and immunofluorescence . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| It is composed of HIF 1alpha and HIF 1beta subunits and its activity depends on the amount of HIF 1alpha , which is tightly controlled by cellular oxygen tension . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a basic helix loop helix transcription factor composed of two subunits , HIF 1alpha and HIF 1beta . ^^^ We have also shown that arsenite specifically induces HIF 1alpha , but not HIF 1beta , protein levels in prostate cancer cells in a mTOR dependent manner . . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimeric basic helix loop helix transcription factor , composed of HIF 1alpha and HIF 1beta / ARNT subunits ; and is involved in tumor growth and angiogenesis . ^^^ Here we demonstrate that arsenite induces the expression of HIF 1alpha but not HIF 1beta subunit in DU 145 human prostate carcinoma cells . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Western blot analyses were used to evaluate HIF 1alpha , HIF 1beta , HIF 2alpha , von Hippel Lindau protein , VEGF , Flt 1 , enolase , and GAPDH . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1alpha expression directly correlated with augmented HIF 1 DNA binding and transcriptional activity of luciferase reporter plasmids , whereas HIF 1beta levels remained unaffected . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , a pivotal transcription factor composed of HIF 1alpha and HIF 1beta subunits , plays a major role in tumor progression by activating a number of genes critically involved in adaptation to hypoxia . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Because hypoxia inducible factor ( HIF 1 ) , a transcription factor composed of oxygen labile HIF 1alpha and constitutive HIF 1beta subunits , regulates the physiologic / pathophysiologic response to hypoxia and ischemia , we examined the HIF 1 response in two rat models of gut ischemia reperfusion . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1alpha expression was sensitive to changes in ambient oxygen concentrations , while its dimerization partner HIF 1beta was constitutively expressed . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Under hypoxic conditions , HIF 1alpha is stabilized and enters the nucleus , to form a dimer with HIF 1beta , where it induces the expression of its target genes . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| It consists of two constitutively produced subunits : HIF 1alpha and HIF 1beta . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 consists of an oxygen sensitive HIF 1alpha and oxygen insensitive HIF 1beta . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| It was shown previously that HIF 1beta protein expression is nearly ubiquitous in newborn kidney and that HIF 1beta dimerizes with either HIF 1alpha or 2alpha . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is composed of HIF 1alpha and HIF 1beta subunits . ^^^ SU 5416 specifically decreased HIF 1alpha , but not HIF 1beta protein levels . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Seal lung and skeletal muscle tissues showed the highest relative levels of HIF 1alpha protein expression , with heart muscle showing significantly lower levels , and levels of HIF 1beta protein expression paralleled this situation . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The aim of the present study is to investigate whether hypoxia inducible factors ( HIF 1alpha , HIF 2alpha , HIF 1beta ) are involved in enhanced apoptosis in the human placenta from pregnancies with fetal growth restriction ( FGR ) . ^^^ The expressions of HIF 1alpha , HIF 2alpha , and HIF 1beta were examined by immunohistochemical analysis . ^^^ There were no significant differences in the intensity of the staining for HIF 1alpha and HIF 1beta expressions between two groups , while HIF 2alpha was overexpressed in the placenta from pregnancies with FGR group ( P < 0 . 05 ) . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor ( HIF ) 1 , a heterodimeric transcription factor composed of HIF 1alpha and HIF 1beta subunits coordinates pathophysiologic responses toward decreased oxygen availability . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Expression of subunits of HIF 1 , HIF 1alpha , and HIF 1beta was examined by Western blot using specific antibodies . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , a transcription factor composed of two subunits ( HIF 1alpha and HIF 1beta ) , initially described as a mediator of adaptive responses to changes in tissue oxygenation , has been shown to be activated in an oxygen independent manner . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| This effort led to the discovery of 103D5R , a novel small molecule inhibitor of HIF 1alpha . 103D5R markedly decreased HIF 1alpha protein levels induced by hypoxia or cobaltous ions in a dose and time dependent manner , whereas minimally affecting global cellular protein expression levels , including that of control proteins such as HIF 1beta , IkappaBalpha , and beta actin . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The mechanism of this hypoxia induced tolerance correlates with the induction of the hypoxia inducible factor ( HIF ) , a transcription factor heterodimeric complex composed of inducible HIF 1alpha and constitutive HIF 1beta proteins that bind to the hypoxia response elements in a number of HIF target genes . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , the master regulator of transcriptional responses to reduced oxygen tension ( hypoxia ) in mammalian cells , consists of one HIF 1alpha and one HIF 1beta subunit . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF is a heterodimer consisting of the O 2 regulated subunit , HIF 1alpha , and the constitutively expressed aryl hydrocarbon receptor nuclear translocator , HIF 1beta . ^^^ Under hypoxic conditions , HIF 1alpha is stable , accumulates , and migrates to the nucleus where it binds to HIF 1beta to form the complex ( HIF 1alpha + HIF 1beta ) . ^^^ Transcription is initiated by the binding of the complex ( HIF 1alpha + HIF 1beta ) to hypoxia responsive elements ( HREs ) . ^^^ The complex [ ( HIF 1alpha + HIF 1beta ) + HREs ] stimulates the expression of genes involved in angiogenesis , anaerobic metabolism , vascular permeability , and inflammation . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Serum / IGF 1 induced increases in HIF 1alpha protein that temporally paralleled increases in VEGF mRNA , whereas HIF 1beta levels were unaffected . ^^^ Topotecan blocked the IGF 1 stimulated increase in HIF 1alpha but not HIF 1beta , and this resulted in a decrease in VEGF in four neuroblastoma cell lines tested . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In an attempt to understand the mechanism of apigenin inhibited VEGF expression , we found that apigenin inhibited VEGF transcriptional activation through the hypoxia inducible factor 1 ( HIF 1 ) binding site and specifically decreased HIF 1alpha but not HIF 1beta subunit expression in the cells . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| It consists of a regulatory subunit HIF 1alpha , which accumulates under hypoxic conditions , and a constitutively expressed subunit , HIF 1beta . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 , an alphabeta heterodimeric transcription factor , consists of a constitutively expressed HIF 1beta subunit and a hypoxia inducible HIF 1alpha subunit , and contributes to hypoxia mediated tumor angiogenesis . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Expression of subunits of HIF 1 , HIF 1alpha , and HIF 1beta were examined by Western blot using specific antibodies . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1alpha is a transcription factor that during hypoxia binds with a second protein ( HIF 1beta ) in the nucleus to promoter elements in hypoxia responsive target genes . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimer consisting of the HIF 1alpha and HIF 1beta subunits , and HIF 1alpha is the unique oxygen regulated subunit that determines HIF 1 activity . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , which consists of oxygen sensitive HIF 1alpha and constitutively expressed HIF 1beta subunits , activates transcription of genes encoding proteins that mediate adaptive responses to reduced oxygen availability . ^^^ Interestingly , both HIF 1alpha and HIF 1beta interacted with the IPAS proteins . ^^^ Collectively , these results suggest that human IPAS proteins inhibit angiogenesis by binding to and inhibiting HIF 1alpha and HIF 1beta . . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is composed of two subunits , HIF 1alpha and HIF 1beta , and HIF 1 activity depends mainly on the intracellular level of HIF 1alpha protein , which is regulated to be in inverse relation to the oxygen concentration by an oxygen dependent enzyme , prolyl hydroxylase 2 ( PHD 2 ) . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| To identify inhibitors of HIF 1 DNA binding activity , we expressed truncated HIF 1alpha and HIF 1beta proteins containing the basic helix loop helix and PAS domains . ^^^ Expressed recombinant HIF 1alpha and HIF 1beta proteins induced a specific DNA binding activity to a double stranded oligonucleotide containing a canonical hypoxia responsive element ( HRE ) . ^^^ Electrophoretic mobility shift assay experiments showed that NSC 13502 inhibited binding of HIF 1alpha and HIF 1beta proteins to a HRE sequence but not binding of the corresponding proteins to activator protein 1 ( AP 1 ) or nuclear factor kappaB ( NF kappaB ) consensus sequences . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In mammalian cells , the transcriptional response to oxygen deprivation is largely mediated by hypoxia inducible factor 1 ( HIF 1 ) , a heterodimer composed of HIF 1alpha and HIF 1beta subunits . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Knockdown of HIF 1alpha or HIF 1beta levels in HPASMC with specific small interfering RNAs inhibited FGF 2 stimulated proliferation of HPASMC incubated in either 5 % or 20 % O ( 2 ) but failed to inhibit the comitogenic effect of hypoxia . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The expression patterns of HIF 1alpha and HIF 1beta factors followed , in general , the Hypoxyprobe 1 marked regions . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that macrophage differentiation of THP 1 cells or monocytes from peripheral blood induces increased expression of both HIF 1alpha and HIF 1beta as well as increased HIF 1 transcriptional activity leading to increased expression of HIF 1 target genes . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a heterodimeric transcription factor composed of HIF 1alpha and HIF 1beta subunits and involved in the regulation of gene expression in adaptive response to hypoxia . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| BDNF induced increases in VEGF and HIF 1alpha protein , whereas HIF 1beta levels were unaffected . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a heterodimeric transcription factor composed of HIF 1alpha and HIF 1beta subunits that functions as a master regulator of oxygen homeostasis . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is a heterodimer composed of an oxygen regulated subunit known as HIF 1alpha and a constitutively expressed HIF 1beta subunit . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Several domains of HIF 1alpha mediate protein protein interaction , which is essential for the formation of the active heterodimer with HIF 1beta . ^^^ HIF 1alpha and HIF 1beta contain two Per Arnt Sim ( PAS ) domains , A and B , both of which appear to be important for heterodimer formation . ^^^ In an attempt to identify small molecule inhibitors of the PAS A domain of HIF 1 we expressed proteins containing amino acids 86 165 of HIF 1alpha and amino acids 159 240 of HIF 1beta fused to a His or FLAG tag , respectively . ^^^ An ELISA based on the interaction between FLAG HIF 1beta PAS A and HIF 1alpha PAS A His was developed and used to screen libraries of synthetic compounds . ^^^ NSC 50352 specifically inhibited PAS A dependent interaction between HIF 1alpha and HIF 1beta , but not the interaction mediated by unrelated domains . ^^^ |
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| Interacting proteins: Q53F30 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Furthermore , 1 and 2 suppressed the accumulation of HIF 1alpha protein in a dose dependent manner , but not the HIF 1beta protein and inhibited expression of vascular endothelial growth factor ( VEGF ) by hypoxia . . ^^^ |
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