| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Sequence determinants in hypoxia inducible factor 1alpha for hydroxylation by the prolyl hydroxylases PHD 1 , PHD 2 , and PHD 3 . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Three human oxygen dependent HIF 1 alpha prolyl hydroxylases ( PHD 1 , PHD 2 , and PHD 3 ) function as oxygen sensors in vivo . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Three HIF alpha prolyl 4 hydroxylases ( PHDs ) ( named PHD 1 , PHD 2 , and PHD 3 ) effect the proteasome mediated degradation of HIF by catalyzing the hydroxylation of key proline residues in the HIF 1 alpha subunit under normoxic conditions . ^^^ Treatment of human cardiac myocytes , smooth muscle cells , and endothelial cells with hypoxia or CoCl ( 2 ) , a hypoxia mimic , resulted in a significant time dependent increase in PHD 3 , but not PHD 1 or PHD 2 , mRNA levels , which correlated with an increase in HIF 1 alpha protein expression . ^^^ Overexpression studies revealed that PHD 3 levels influence HIF 1 alpha stability in both normoxic and hypoxic conditions , suggesting that PHD 3 may participate in a feedback loop controlling HIF activity . . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Here we show that specific ' silencing ' of PHD 2 with short interfering RNAs is sufficient to stabilize and activate HIF 1alpha in normoxia in all the human cells investigated . ' Silencing ' of PHD 1 and PHD 3 has no effect on the stability of HIF 1alpha either in normoxia or upon re oxygenation of cells briefly exposed to hypoxia . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| RNA interference directed against PHD 2 , but not PHD 1 or PHD 3 , is sufficient to stabilize HIF 1alpha in normoxia . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Although hypoxia / DFX induced PHD 3 , we excluded the PHD / VHL pathway in the regulation of HIF 1alpha under hypoxia / DFX . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| With increasing age we found a decreased expression of HIF 1alpha , which correlated to an increased PHD 3 expression in mouse and human heart . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Semiquantitative immunohistochemical analysis was used to assess the expression of the androgen receptor , VEGF and HIF 1a and 2a , and their regulatory prolyl hydroxylase enzymes ( PHD 1 , PHD 2 , and PHD 3 ) . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor ( HIF ) alpha subunits ( HIF 1alpha , HIF 2alpha and HIF 3alpha ) , which play a pivotal role during the development of hypoxia induced pulmonary hypertension ( HPH ) , are regulated through post translational hydroxylation by their three prolyl hydroxylase domain containing proteins ( PHD 1 , PHD 2 and PHD 3 ) . ^^^ In hypoxic animals , HIF 1alpha proteins negatively correlated with PHD 2 proteins , whereas HIF 2alpha and HIF 3alpha proteins showed negative correlations with PHD 3 and PHD 1 proteins , respectively . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| As demonstrated under chronically hypoxic conditions in vitro , PHD 2 and PHD 3 show a transient maximum but remain up regulated over more than 10 days , suggesting a feedback down regulation of HIF 1alpha which then levels off at a novel set point . ^^^ Indeed , hypoxic induction of PHD 2 and PHD 3 is paralleled by the attenuation of endogenous HIF 1alpha . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Three HIF prolyl hydroxylases ( EGLN 1 , EGLN 2 , and EGLN 3 ) have been identified in mammals , among which EGLN 1 and EGLN 3 are hypoxia inducible at their mRNA levels in an HIF 1alpha dependent manner . ^^^ |
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| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H6Z9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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