| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF prolyl hydroxylase 2 is the key oxygen sensor setting low steady state levels of HIF 1alpha in normoxia . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxic preconditioning produces differential expression of hypoxia inducible factor 1alpha ( HIF 1alpha ) and its regulatory enzyme HIF prolyl hydroxylase 2 in neonatal rat brain . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We report here that PHD 2 has the highest specific activity toward the primary hydroxylation site of HIF 1alpha . ^^^ Sequence determinants in hypoxia inducible factor 1alpha for hydroxylation by the prolyl hydroxylases PHD 1 , PHD 2 , and PHD 3 . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Finally , we will present recent advances into oxygen sensing , in particular the HIF hydroxylases that govern HIF 1alpha instability ( PHD 2 ) or inactivation ( FIH 1 ) . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Three human oxygen dependent HIF 1 alpha prolyl hydroxylases ( PHD 1 , PHD 2 , and PHD 3 ) function as oxygen sensors in vivo . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Three HIF alpha prolyl 4 hydroxylases ( PHDs ) ( named PHD 1 , PHD 2 , and PHD 3 ) effect the proteasome mediated degradation of HIF by catalyzing the hydroxylation of key proline residues in the HIF 1 alpha subunit under normoxic conditions . ^^^ Treatment of human cardiac myocytes , smooth muscle cells , and endothelial cells with hypoxia or CoCl ( 2 ) , a hypoxia mimic , resulted in a significant time dependent increase in PHD 3 , but not PHD 1 or PHD 2 , mRNA levels , which correlated with an increase in HIF 1 alpha protein expression . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| RNA interference directed against PHD 2 , but not PHD 1 or PHD 3 , is sufficient to stabilize HIF 1alpha in normoxia . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| PHD 2 is the most active of three PHD isoforms in hydroxylating HIF 1alpha . ^^^ Overexpression of PHD 2 lacking the MYND domain caused a greater reduction in the stability and function of HIF 1alpha than did overexpression of wild type PHD 2 , indicating that the MYND domain also inhibits the catalytic activity of PHD 2 in vivo . . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Transfection experiments demonstrated that especially HIF 3alpha and HIF 2alpha , and to a lesser extent HIF 1alpha , contribute to the induction of IGFBP 1 mRNA expression in isolated hepatocytes , whereas experiments with vectors for the HIF prolyl hydroxylases ( PHD ) indicated a major role of PHD 2 in destabilization of HIFs , attenuating the induction of IGFBP 1 under venous pO ( 2 ) . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 is composed of two subunits , HIF 1alpha and HIF 1beta , and HIF 1 activity depends mainly on the intracellular level of HIF 1alpha protein , which is regulated to be in inverse relation to the oxygen concentration by an oxygen dependent enzyme , prolyl hydroxylase 2 ( PHD 2 ) . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Semiquantitative immunohistochemical analysis was used to assess the expression of the androgen receptor , VEGF and HIF 1a and 2a , and their regulatory prolyl hydroxylase enzymes ( PHD 1 , PHD 2 , and PHD 3 ) . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1 preservation was achieved by using small interfering RNA ( siRNA ) to silence murine HIF 1alpha prolyl 4 hydroxylase 2 ( PHD 2 ) . ^^^ PHD 2 silencing in normoxic EC stabilized HIF 1alpha protein levels while significantly increasing HIF 1 transcriptional activity and iNOS mRNA expression . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| This study was designed to analyze the correlation of the expression and subcellular localization of PHD 2 with the pathologic features of human carcinomas and HIF 1alpha expression . ^^^ Further studies included PHD 2 mRNA detection and HIF 1alpha immunohistochemistry from HNSCC specimens as well as PHD 2 immunocytochemistry from HNSCC derived cell lines . ^^^ Finally , although most of the tumor regions with high PHD 2 expression show down regulated HIF 1alpha , regions with simultaneous HIF 1alpha and PHD 2 expression could be detected . ^^^ Furthermore , they imply that even the elevated PHD 2 levels are not sufficient to down regulate HIF 1alpha in some tumors . . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor ( HIF ) alpha subunits ( HIF 1alpha , HIF 2alpha and HIF 3alpha ) , which play a pivotal role during the development of hypoxia induced pulmonary hypertension ( HPH ) , are regulated through post translational hydroxylation by their three prolyl hydroxylase domain containing proteins ( PHD 1 , PHD 2 and PHD 3 ) . ^^^ In hypoxic animals , HIF 1alpha proteins negatively correlated with PHD 2 proteins , whereas HIF 2alpha and HIF 3alpha proteins showed negative correlations with PHD 3 and PHD 1 proteins , respectively . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| As demonstrated under chronically hypoxic conditions in vitro , PHD 2 and PHD 3 show a transient maximum but remain up regulated over more than 10 days , suggesting a feedback down regulation of HIF 1alpha which then levels off at a novel set point . ^^^ Indeed , hypoxic induction of PHD 2 and PHD 3 is paralleled by the attenuation of endogenous HIF 1alpha . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In particular , we demonstrate that TGFbeta markedly and specifically decreases both mRNA and protein levels of a HIF 1alpha associated prolyl hydroxylase ( PHD ) , PHD 2 , through the Smad signaling pathway . ^^^ Moreover , inhibition of the TGFbeta 1 converting enzyme , furin , resulted in increased PHD 2 expression , and decreased basal HIF 1alpha and VEGF levels , suggesting that endogenous production of bioactive TGFbeta 1 efficiently regulates HIF 1 targeted genes . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The C terminal domain of Falkor , isolated from the GSE library , has significant homology to a known human and rat cell growth regulator , SM 20 , and to the C . elegans protein EGL 9 , recently shown to modify the Hypoxia Inducible Factor 1alpha . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| ANG 2 induces HIF 1alpha by a posttranscriptional mechanism suggesting that SM 20 down regulation leads to stabilization of HIF 1 . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Suppression of hypoxia inducible factor 1alpha ( HIF 1alpha ) transcriptional activity by the HIF prolyl hydroxylase EGLN 1 . ^^^ Three HIF prolyl hydroxylases ( EGLN 1 , EGLN 2 , and EGLN 3 ) have been identified in mammals , among which EGLN 1 and EGLN 3 are hypoxia inducible at their mRNA levels in an HIF 1alpha dependent manner . ^^^ In this study , we demonstrated that apart from promoting HIF 1alpha proteolysis in normoxia , EGLN 1 specifically represses HIF 1alpha transcriptional activity in hypoxia . ^^^ Ectopic expression of EGLN 1 inhibited HIF 1alpha transcriptional activity without altering its protein levels in a von Hippel Lindau deficient cell line , indicating a discrete activity of EGLN 1 in transcriptional repression . ^^^ Conversely , silencing of EGLN 1 expression augmented HIF 1alpha transcriptional activity and its target gene expression in hypoxia . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Induction of human endometrial cancer cell senescence through modulation of HIF 1alpha activity by EGLN 1 . ^^^ |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9GZT9 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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