| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , a DNA binding complex implicated in the regulation of gene expression by oxygen , has been shown to consist of a heterodimer of two basic helix loop helix Per AHR ARNT Sim ( PAS ) proteins , HIF 1alpha , and HIF 1beta . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In this study , we report structural features of the HIF 1alpha subunit that are required for heterodimerization , DNA binding , and transactivation . ^^^ The HIF 1alpha and HIF 1beta ( ARNT ; aryl hydrocarbon receptor nuclear translocator ) subunits were coimmunoprecipitated from nuclear extracts , indicating that these proteins heterodimerize in the absence of DNA . ^^^ In vitro translated HIF 1alpha and HIF 1beta generated a HIF 1 / DNA complex with similar electrophoretic mobility and sequence specificity as HIF 1 present in nuclear extracts from hypoxic cells . ^^^ Compared to 826 amino acid , full length HIF 1alpha , amino acids 1 166 mediated heterodimerization with HIF 1beta ( ARNT ) , but amino acids 1 390 were required for optimal DNA binding . ^^^ A deletion involving the basic domain of HIF 1alpha eliminated DNA binding without affecting heterodimerization . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that HIF 1 alpha required Arnt for DNA binding in vitro and functional activity in vivo . ^^^ Consistent with these observations , activation of HIF 1 alpha function in vivo or overexpression of HIF 1 alpha inhibited ligand dependent induction of DNA binding activity by the dioxin receptor and dioxin receptor function on minimal reporter gene constructs . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxic conditions lead to the formation of a DNA binding complex containing both HIF 1 alpha and p300 / CBP . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Like HIF 1alpha , EPAS 1 binds to and activates transcription from a DNA element originally isolated from the erythropoietin gene and containing the sequence 5 ' GCCCTACGTGCTGTCTCA 3 ' . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Structural analysis of HIF 1 alpha revealed that dimerization with HIF 1 beta ( ARNT ) requires the HLH and PAS domains , DNA binding is mediated by the basic domain , and that HIF 1 alpha contains a carboxyl terminal transactivation domain . ^^^ In HeLa cells , the levels of HIF 1 alpha and HIF 1 beta protein and HIF 1 DNA binding activity increased exponentially as cellular oxygen tension decreased , with maximum values at 0 . 5 % oxygen and half maximal values at 1 . 5 to 2 % oxygen . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In contrast to the reported very low basal HIF 1 alpha mRNA levels , however , we detected HIF 1 alpha mRNA in several cell lines cultured under normoxic conditions , although no HIF 1 DNA binding activity was observed . ^^^ Following hypoxic induction , VEGF mRNA levels and HIF 1 DNA binding activity increased , but HIF 1 alpha mRNA levels remained largely unchanged . ^^^ One possible explanation for this discrepancy might be that HIF 1 DNA binding activity does not follow HIF 1 alpha mRNA kinetics . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation studies , yeast two hybrid analysis , and transient transfection experiments demonstrated that MOP 1 and MOP 2 dimerize with ARNT and that these complexes are transcriptionally active at defined DNA enhancer sequences in vivo . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) , a heterodimeric DNA binding complex composed of two basic helix loop helix Per AHR ARNT Sim proteins ( HIF 1alpha and 1beta ) , is a key component of a widely operative transcriptional response activated by hypoxia , cobaltous ions , and iron chelation . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Moreover , the high affinity DNA binding form of the HIF 1alpha Arnt complex was only generated by forms of Arnt capable of eliciting the allosteric change in conformation . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| DNA binding studies using nuclear extracts from the mouse hepatoma cell line Hepa 1 and the ARNT / HIF 1beta deficient subline Hepa1C4 , as well as antibodies raised against HIF 1alpha and ARNT / HIF 1beta confirmed that HIF 1 binds the Tf HBSs . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning of HIF 1 has shown that this widely expressed , DNA binding transcription factor is a heterodimer of two proteins , HIF 1 alpha and HIF 1 beta . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| This DNA binding complex was identified as HIF 1 using antibodies directed against HIF 1 alpha and HIF 1 beta . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| An important mediator is hypoxia inducible factor 1 ( HIF 1 ) , a DNA binding complex shown to contain at least two basic helix loop helix PAS domain ( bHLH PAS ) proteins , HIF 1alpha and aryl hydrocarbon nuclear receptor translocator ( ARNT ) . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a heterodimeric DNA binding complex composed of two basic helix loop helix PAS proteins : HIF 1 beta / ARNT ( aryl hydrocarbon receptor nuclear translocator ) , which is constitutively expressed , and HIF 1 alpha , which is not present in normoxic cells but induced under hypoxic conditions . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1alpha protein expression and HIF 1 DNA binding activity were detected in normoxic PC 3 cells . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Both NO and CO inhibited HIF 1 DNA binding by abrogating hypoxia induced accumulation of HIF 1alpha protein . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In hypoxic condition , Arnt dimerizes with HIF 1alpha to make HIF 1alpha / Arnt heterodimer which is able to bind hypoxia responsive DNA elements . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Since EPO gene expression is inhibited by the proinflammatory cytokines interleukin 1beta ( IL 1beta ) and tumor necrosis factor alpha ( TNF alpha ) , while no such effect has been reported with respect to the VEGF gene , we investigated the effects of IL 1beta and TNF alpha on the activation of the HIF 1 DNA binding complex and the amount of HIF 1alpha protein in human hepatoma cells in culture . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Despite strong similarities in their activation mechanisms ( e . g . they both undergo rapid hypoxia induced protein stabilization , bind identical target DNA sequences , and induce synthetic reporter genes to similar degrees ) , they are both essential for embryo survival via distinct functions during vascularization ( HIF 1alpha ) or catecholamine production ( HLF ) . ^^^ We report here that DNA binding by HLF , but not by HIF 1alpha , is dependent upon reducing redox conditions . ^^^ Although the N terminal DNA binding domain of HIF 1alpha can function in the absence of Ref 1 , we found that the C terminal region containing the transactivation domain requires Ref 1 for full activity . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In addition , the levels of proteins involved in cellular responses to hypoxia , ROS and nuclear DNA damage ; hypoxia inducible factor 1alpha ( HIF 1alpha ) , p 53 , p 21 and PCNA were also modulated temporally . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| N Acetyl l cysteine , a glutathione ( GSH ) precursor and a potent scavenger of reactive oxygen species , induced HIF 1alpha and ameliorated NF kappaB nuclear abundance and DNA binding activity , respectively , in a dose dependent manner . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Moreover , although HIF 1 alpha mRNA expression was shown to be similar in young and old smooth muscle cells , HIF 1 alpha protein and DNA binding activity were significantly reduced in old versus young smooth muscle cells that were exposed to hypoxia . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We found that epolones ( optimal concentration 4 8 micromol / L ) potently induce HIF 1 alpha protein accumulation and nuclear translocation as well as HIF 1 DNA binding and reporter gene transactivation . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In a genetic model system , 40 % complex 1 inhibited human ape xenomitochondrial cybrids , hypoxic induction of HIF 1alpha was severely reduced , and HIF 1 DNA binding was diminished . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Angiogenesis is induced in a rabbit model of hindlimb ischemia by naked DNA encoding an HIF 1alpha / VP16 hybrid transcription factor . ^^^ METHODS AND RESULTS : A hybrid protein consisting of DNA binding and dimerization domains from the HIF 1alpha subunit and the transactivation domain from herpes simplex virus VP 16 protein was constructed to create a strong , constitutive transcriptional activator . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We demonstrated that S nitrosoglutathione induces HIF 1 alpha accumulation and concomitant DNA binding . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| From these results , we postulate that the specificity of HIF 1 toward its DNA sequence could be driven by the HIF 1alpha subunit . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The mop 1 1 mutation does not alter the extent of DNA methylation in rDNA and centromeric repeats . ^^^ The observation that mop 1 affects paramutation at multiple loci , despite major differences between these loci in their gene structure , correlations with DNA methylation , and stability of the paramutant state , suggests that a common mechanism underlies paramutation . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1alpha was demonstrated by Western blot analysis and HIF 1 DNA binding by gel shift assay . ^^^ Nuclear HIF 1alpha protein levels and HIF 1 binding to DNA were also increased under these conditions . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Second , we examined some aspects of HIF 1 activity regulation in HGF treated cells with the following findings : ( 1 ) the activation of HIF 1 DNA binding was prevented by proteasome blockade , probably because stabilization of the cytosolic alpha subunit protein level is not sufficient to generate a functional form : also under these conditions nuclear protein level of HIF 1alpha did not increase ; ( 2 ) N acetylcysteine , a free radical scavenger , strongly decreased HIF 1 activation suggesting a role of reactive oxygen species in this process ; ( 3 ) the thiol reducing agent dithiothreitol was ineffective . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Interestingly , Ape1 / ref 1 is a multifunctional protein that not only is a DNA repair enzyme , but also functions as a redox factor maintaining transcription factors , such as Fos , Jun , nuclear factor kappaB , PAX ( paired box containing family of genes ) , hypoxia inducible factor lalpha ( HIF 1alpha ) , HIF 1 like factor , and p 53 , in an active reduced state . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| DNA repair redox enzyme apurinic endonuclease in cervical cancer : evaluation of redox control of HIF 1alpha and prognostic significance . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We show that both short ( I . 1 ) and long ( I . 2 ) HIF 1 alpha isoforms display similar DNA binding and transcriptional activities . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Using these cells , we found that normoxically induced HIF 1a is localized to the nucleus , binds DNA , and trans activates reporter and endogenous target genes . ^^^ The MAP kinase inhibitor PD 98059 attenuated HIF 1a protein modifications and trans activation ability but not protein stabilization and DNA binding activity . ^^^ Because overexpressed HIF 1a is fully localized to the nucleus but displays only partial DNA binding and trans activation activity , mitogen activated protein kinase dependent phosphorylation might be required for full HIF 1 activation . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| This cooperative effect is not due to an increase in the nuclear amount of the HIF 1alpha subunit , nor does it require direct binding of c Jun to DNA . c Jun and HIF 1alpha are able to associate in vivo but not in vitro , suggesting that this interaction involves the participation of additional proteins and / or a posttranslational modification of these factors . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Heat induced HIF 1alpha ( a ) accumulated in the nucleus but neither bound to DNA nor trans activated reporter or target gene expression , demonstrating the need for post translational modifications for these functions . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Despite the activation of DNA HIF 1 binding activity under hypoxic conditions , neither an accumulation of HIF 1 alpha nor the activation of HIF 1 was observed following the expression of PLAGL 2 . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| We report here that , whereas serum stimulation can induce a slight accumulation of HIF 1 alpha protein in a PI3K / Akt pathway dependent fashion , hypoxia induces much higher levels of HIF 1 alpha protein and HIF 1 DNA binding activity independently of PI3K and mTOR activity . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In human gliomas and other cancer cell lines , the accumulation of HIF 1alpha protein under aerobic conditions requires the metabolism of glucose to pyruvate that prevents the aerobic degradation of HIF 1alpha protein , activates HIF 1 DNA binding activity , and enhances the expression of several HIF 1 activated genes including erythropoietin , vascular endothelial growth factor , glucose transporter 3 , and aldolase A . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In contrast , NPR overexpression in Hep3B cells enhanced the DNA binding activity of HIF 1alpha by luciferase reporter gene assay . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| This activation correlated with induction of HIF 1alpha expression and DNA binding activity which was blocked by the p 38 inhibitor SB 203580 . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Intramyocardial injection of naked DNA encoding HIF 1alpha / VP16 hybrid to enhance angiogenesis in an acute myocardial infarction model in the rat . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The expression of HIF 1alpha is correlated with the activation of HIF 1 DNA binding activity and the transactivation of a HIF 1 dependent reporter gene . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Analysis of the DNA extracted from a benign or tumorous region of one of these specimens showed that only the wildtype ( nonmutated ) form of the HIF 1alpha gene was amplified from the normal DNA whereas only the mutated form of the HIF 1alpha gene was amplified from the tumor . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Increased HIF 1a protein expression and DNA binding activity were detected in bcl 2 overexpressing cells compared with control cells . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxic induction of hypoxia inducible factor 1alpha and oxygen regulated gene expression in mitochondrial DNA depleted HeLa cells . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Our previous observation implied that the MEK 1 / p42 / p44 MAPK pathway is involved in the hypoxia induced transactivation ability , but not in the stabilization and DNA binding of HIF 1alpha . ^^^ We designed several fusion proteins that contain deletion mutants of HIF 1alpha that is linked to the DNA binding domain of the yeast protein Gal 4 . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Primary evidence for this model comes from the observation that cells treated with complex 1 inhibitors , such as rotenone , or cells that lack mitochondrial DNA ( rho ( 0 ) cells ) fail to generate reactive oxygen species or stabilize HIF 1alpha protein in response to hypoxia . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Specific interactions between three amino acids ( Ser 22 , Ala 25 , Arg 30 ) of the HIF 1alpha subunit and DNA bases were identified . ^^^ These results correlate with the ones obtained when the DNA binding capability of the mutants was assayed by electrophoretic mobility shift assays ( EMSA ) : Arg30Ala and Ala26Glu mutants bind very weakly to HRE while the Ser22Ala mutant has the same binding capacity as the wild type HIF 1alpha . ^^^ Site directed mutagenesis studies of the hypoxia inducible factor 1alpha DNA binding domain . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Here , we show that ionomycin could activate HIF 1 transcriptional activity in a way that is additive to the effect of hypoxia without affecting HIF 1alpha protein level and HIF 1 DNA binding capacity . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Furthermore , statins inhibited DNA binding of hypoxia inducible factor 1alpha . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The normoxic expression of HIF 1alpha in concordance with its DNA binding activity enhances the transcription of target genes such as vascular endothelial growth factor . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In altitude adapted Andean natives , the Epo hypoxic response may be blunted ; however , our data indicate that the DNA sequences of the genes encoding Epo ( including the 3 ' regulatory region ) and HIF 1alpha appear to be conserved . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Agents that did not cause DNA double strand breaks , such as DNA synthesis inhibitors or a DNA single strand break causing agent equally impaired cell growth , independent of the HIF 1alpha genotype . ^^^ Thus , hypoxia independent basal HIF 1alpha expression in tumor cells , as known from untransformed embryonic stem cells , is sufficient to induce target gene expression , probably including DNA double strand break repair enzymes . . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia inducible factor 1 ( HIF 1 ) is a DNA transcription factor composed of two subunits , one of which is regulated by hypoxia ( HIF1alpha , encoded by HIF1A ) . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The signal transduction pathways involved in HIF 1alpha stabilization , HIF 1 DNA binding and transactivation by NO and hypoxia in microvascular endothelium remains unknown . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Using rat cardiac myocytes in vitro , we show that morphine : ( 1 ) decreases hypoxia induced VEGF ( 121 ) and VEGF ( 165 ) mRNA expression and VEGF protein concentration through an opioid receptor mechanism ; ( 2 ) decreases HIF 1alpha protein expression ( immunoblot ) and nuclear protein binding to the VEGF HIF 1alpha DNA response element ( EMSA ) ; and ( 3 ) inhibits phospho Erk 1 , 2 MAP kinase ( immunoblot ) and phospho Akt kinase activity ( immunoblot ) . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Furthermore , these inhibitors decreased hypoxia inducible factor 1alpha ( HIF 1alpha ) DNA binding , which suggests that the PDGF / PDGF beta receptor / Akt pathway induces downstream HIF 1alpha gene transcription . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The amplified products were confirmed as the cDNA of HIF 1alpha by DNA sequencing , and pcDNA3 . 1+ HIF 1alpha obtained was verified by endonuclease digestion , being capable of expression in HEK 293 cells . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| PAS regions of Arnt , DR , and HIF 1alpha and evaluated the contribution of the PAS domains to DNA binding in vitro . ^^^ Formation of stable protein DNA complexes by DR / Arnt and HIF 1alpha / Arnt heterodimers with their cognate DNA sequences required the PAS A domains and exhibited KD values of 0 . 4 nM and approximately 50 nM , respectively . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The HIF 1alpha and HIF 2alpha subunits are structurally similar in their DNA binding and dimerization domains but differ in their transactivation domains , implying they may have unique target genes . ^^^ To address this discrepancy , and to identify specific HIF 2alpha target genes , we used DNA microarray analysis to evaluate hypoxic gene induction in cells expressing HIF 2alpha but not HIF 1alpha . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Besides its function in DNA repair , APE serves to maintain several transcription factors in an active reduced state such as c Fos , c Jun , NF kappaB , p 53 and HIF 1alpha , all of which have been shown to play a role in tumorigenesis . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Treatment of cells with PI 3K / Akt inhibitor ( LY 294002 ) and tyrosine protein kinase inhibitor ( genistein ) significantly attenuated hypoxia induced PAI 1 mRNA and protein expression as well as promoter activation , apparently via an inhibition of the hypoxia induced stabilization of HIF 1alpha protein , attenuation of the steady state level of HIF 1alpha mRNA , and its DNA binding activity . ^^^ Even though disruption of ERK1 / 2 signaling pathway by PD 98059 abolished hypoxia induced PAI 1 promoter activation and mRNA / protein expression in keloid fibroblasts , it did not inhibit the hypoxia mediated stabilization of HIF 1alpha protein and the steady state level of HIF 1alpha mRNA nor its DNA binding activity . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1alpha expression directly correlated with augmented HIF 1 DNA binding and transcriptional activity of luciferase reporter plasmids , whereas HIF 1beta levels remained unaffected . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Under hypoxic condition , the expression and activity of DNA PK were markedly induced with a concurrent increase in HIF 1alpha expression . ^^^ Our result also demonstrated that DNA PK could directly interact with HIF 1 , and especially DNA PKcs , the catalytic subunit of DNA PK , could be involved in phosphorylation of HIF 1alpha , suggesting the possibility that the enhanced expression of DNA PK under hypoxic condition might attribute to modulate HIF 1alpha stabilization . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Electrophoretic mobility gel shift assay demonstrated significant enhancement in DNA binding activity of hypoxia inducible factor 1alpha ( HIF 1alpha ) and activator protein 1 ( AP 1 ) in nuclear extracts from CoCl 2 treated hearts . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Decreasing the intracellular calcium level by BAPTA ( 1 , 2 bis ( 2 aminophenoxy ) ethane N , N , N ' , N ' tetraacetic acid ) induced HIF 1alpha nuclear accumulation and enhanced HIF 1 DNA binding within 1 h of incubation . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Having established DNA binding potential at least in vitro , we subsequently analyzed three proteins that have been shown to be regulated by HIF alpha as downstream , molecular markers of HIF 1alpha activity in vivo . ^^^ In summary , HIF 1alpha protein overexpressed in preeclamptic placentae is capable of binding to its DNA recognition sequence in vitro , and modulates gene expression in vivo . . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Using stringent RNA screening , DNA array and Northern assay , we report significant down regulation in the expression of betaAPP and hypoxia inducible factor 1 alpha ( HIF 1alpha ) , and a marked up regulation in the expression of glial specific markers that include S100beta protein , glycerol phosphate dehydrogenase , and glial fibrillary acidic protein . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| PCR studies using primers based on sequences from mouse HIF 1alpha exons 3 , 4 , 5 , 6 , 9 , 10 , 11 , 12 and 15 showed that DNA from seal lung generated PCR products similar to those from mouse DNA . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| METHODS : A series of 15 metastatic androgen independent prostate cancers were examined for mutations in the oxygen dependent domain ( ODD ) of HIF 1alpha by PCR amplification and DNA sequencing . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| By contrast , HIF 1alpha VP 16 expressing HT 22 cells were more resistant to DNA damage ( induced by camptothecin ) or endoplasmic reticulum stress ( induced by thapsigargin and tunicamycin ) than were VP 16 expressing cells , and suppression of HIF 1alpha expression using RNA interference rendered HT 22 cells more sensitive to death induced by DNA damage or endoplasmic reticulum stress . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Among the differentially expressed genes , we chose for further validation histone deacetylase 1 ( HDAC 1 ) , von Hippel Lindau and decorin as upregulated genes , and Sp 1 , hypoxia inducible factor 1 alpha and DNA primase as downregulated genes . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The hypoxia inducible factors 1alpha ( HIF 1alpha ) and 2alpha ( HIF 2alpha ) are key regulators of the transcriptional response to low oxygen and are closely related in domain architecture , DNA binding , and activation mechanisms . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| PATIENTS AND METHODS : Paraffin wax embedded tissue from 140 patients with superficial primary urothelial bladder carcinoma was immunostained for HIF 1alpha , Ki 67 , single stranded DNA antibody for apoptotic cells , p 53 , bcl 2 , vascular endothelial growth factor and CD 31 antigen . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Hypoxia induced changes in ATII homeostasis are thought to proceed primarily via activation of hypoxia inducible factor ( HIF ) 1alpha , because hypoxia increased HIF 1alpha protein expression , nuclear translocation , and transactivation of its specific DNA binding domain , the hypoxia responsive element ( HRE ) . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Additionally , 2C11 mRNA was measured by Northern blotting , and binding of HIF 1alpha to 2C11 promoter was evaluated using electrophoretic mobility shift assay ( EMSA ) with 2C11 promoter DNA containing putative HIF binding sites . ^^^ The increase in 2C11 expression was preceded by an increase in HIF 1alpha protein at 1 h of hypoxia , and EMSA showed a specific and direct interaction between 2C11 promoter DNA and HIF 1alpha in nuclear extracts from astrocytes . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Inhibition of DNA binding by expression of either STAT 3 or HIF 1alpha dominant negative mutants significantly reduces VEGF expression . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Moreover , exercise induced an increase in the DNA binding activity of HIF 1alpha . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Three weeks later , pigs were randomized to receive ( 1 ) adenovirus encoding HIF 1alpha ( Ad2 / HIF 1alpha VP 16 10 ( 10 ) particles ) ; ( 2 ) plasmid DNA encoding HIF 1alpha ( pHIF 1alpha NFkappaB 500 microg ) ; ( 3 ) pHIF 1alpha NFkappaB 2500 microg ; and ( 4 ) adenoviral control ( Ad2 / CMV empty vector 10 ( 10 ) particles ) . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Results indicate that the aging process induces the activation of HIF 1alpha , which is accompanied by increased HIF 1 DNA binding . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Bacterially produced human HIF 1alpha is competent for heterodimerization and specific DNA binding . ^^^ These data indicate that post translational modification of HIF 1alpha is not essential for its interaction with ARNT and DNA , and provide an in vitro system for the characterization of the molecular properties of HIF 1alpha . . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| As shown in a 143B subclone deficient in mitochondrial DNA ( 206rho0 ) , genetic inactivation of the ETC led to similar responses with respect to HIF 1alpha regulation as ETC inhibitors . ^^^ |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The putative amino acid sequence of ringed seal ( Phoca hispida ) hypoxia inducible factor 1alpha ( HIF 1alpha ) derived from DNA sequence analysis of the single copy gene has been investigated . ^^^ Some of these results indicate that seal HIF 1alpha protein can bind HIF Ibeta , DNA , transcriptional coactivators , and von Hippel Lindau protein ( pVHL ) . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| In this study , we investigated HIF 1alpha naked DNA induced angiogenesis in a cerebral ischemic model in vivo . ^^^ METHODS : We utilized a rat encephalo myo synangiosis ( EMS ) model and inoculated HIF 1alpha DNA into the brain surface or the temporal muscle . ^^^ RESULTS : A histological section treated with HIF 1alpha DNA showed an increased expression of HIF 1 a and VEGF with collateral circulation , in comparison with control DNA ( p < 0 . 01 ) . ^^^ Hypoxia inducible factor 1alpha DNA induced angiogenesis in a rat cerebral ischemia model . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| These results suggest that reciprocal activation between HIF 1alpha and iNOS mediates persistent DNA damage , which induces tumor invasiveness via mutations , resulting in poor prognosis . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| DNA was genotyped for the presence of a single nucleotide ( C to T ) polymorphism that changes residue 582 of HIF 1alpha from proline to serine . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The recently identified third member of the human HIF alpha family , HIF 3alpha , produces multiple splice variants that contain extra DNA binding elements and protein protein interaction motifs not found in HIF 1alpha or HIF 2alpha . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| To identify inhibitors of HIF 1 DNA binding activity , we expressed truncated HIF 1alpha and HIF 1beta proteins containing the basic helix loop helix and PAS domains . ^^^ Expressed recombinant HIF 1alpha and HIF 1beta proteins induced a specific DNA binding activity to a double stranded oligonucleotide containing a canonical hypoxia responsive element ( HRE ) . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Oligonucleotides representing this region of the HIF 1alpha promoter were analyzed by electrophoretic mobility shift , chemical probing , circular dichroism , and DNA polymerase arrest assays . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| DNA from these tumors was extracted from microdissected paraffin material and , after nested polymerase chain reaction , sequence analysis was performed to detect hif 1alpha ODD mutations . ^^^ All 24 diffuse and perinecrotic HIF 1alpha positive breast cancers showed wild type DNA sequences in the ODD domain . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Here we have characterized crucian carp HIF 1alpha , and measured mRNA , protein and DNA binding levels during hypoxia exposure in crucian carp gills . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Patient subsets were examined for tumor oxygen tension using an Eppendorf electrode , S phase DNA content , or change in HIF 1alpha expression over the course of treatment . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| To find out whether HIF 1 function is involved in gene expression changes associated with temperature acclimation as well as in hypoxia adaptation in poikilotherms , we studied HIF 1 DNA binding activity and HIF 1alpha expression in normoxia and during hypoxia ( 0 . 7 mg l ( 1 ) O 2 ) in crucian carp at temperatures of 26 , 18 and 8 degrees C . ^^^ Although HIF 1alpha mRNA levels remained unaltered , cold acclimation ( 8 degrees C ) increased HIF 1alpha protein amounts in the liver , gills and heart and HIF 1 DNA binding activity in the heart , gills and kidney of crucian carp by two to threefold compared to warm acclimated fish ( 26 degrees C ) . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The HIF 1alpha and HIF 2alpha subunits are structurally similar in their DNA binding and dimerization domains but differ in their transactivation domains , implying they may have unique target genes and require distinct transcriptional cofactors . ^^^ Using DNA microarray analysis of hypoxia inducible genes in wild type ( WT ) , Hif 1alpha ( ) ( / ) ( ) , and Hif 2alpha ( ) ( / ) ( ) ES cells , we show that HIF 1alpha induces a large number of both confirmed and novel hypoxia inducible genes , while HIF 2alpha does not activate any of its previously described targets . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| HIF 1alpha DNA binding and transcriptional activity were assessed . ^^^ HBO 2 increased HIF 1alpha DNA binding 5 . 7 ( + / 1 . 2 ) fold relative to controls ( p < 0 . 01 ) ; however , a parallel increase in HIF functional transcriptional activity did not occur . ^^^ HBO 2 increases HIF 1alpha protein levels and DNA binding without a corresponding increase in transcriptional activity . . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| The post translational modification of HIF 1a , the oxygen sensitive subunit of HIF 1 , regulates stabilization , nuclear translocation , DNA binding activity , and transcriptional activity of the protein . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| Among others , recent studies indicated that transforming growth factor ( TGF ) beta increases the expression of the regulatory HIF 1alpha subunit , and induces HIF 1 DNA binding activity . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| These data indicate that all three proteins , CBP , SRC 1 , and Ref 1 , are important components of the hypoxia signaling pathway and have a common function in regulation of HIF 1alpha function in hypoxic cells . ^^^ Given the absence of cysteine residues in one of the Ref 1 regulated transactivation domains of HIF 1alpha , it is thus possible that Ref 1 functions in hypoxic cells by targeting critical steps in the recruitment of the CBP SRC 1 coactivator complex . . ^^^ |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P27695 and Q16665 |
Pubmed |
SVM Score :0.0 |
| NA |
|