| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.89324318 |
| Interestingly , we observed that HIF 1 alpha was associated with the molecular chaperone hsp 90 . 0.89324318^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Hsp 90 client proteins include mutated p 53 , Bcr Abl , Raf 1 , Akt , ErbB 2 and hypoxia inducible factor 1 alpha ( HIF 1 alpha ) . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Interaction of the PAS B domain with HSP 90 accelerates hypoxia inducible factor 1alpha stabilization . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Hsp 90 client proteins include mutated p 53 , Bcr Abl , Raf 1 , Akt , HER2 / Neu ( ErbB 2 ) and hypoxia inducible factor 1alpha ( HIF 1alpha ) . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Hsp 90 client proteins include mutated p 53 , Bcr Abl , Raf 1 , Akt , HER2 / Neu ( ErbB 2 ) , and HIF 1alpha . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| HIF 1 alpha also associates with the molecular chaperone Hsp 90 . ^^^ To examine the role of Hsp 90 in HIF 1 alpha function , we used renal carcinoma cell ( RCC ) lines that lack functional VHL and express stable HIF 1 alpha protein under normoxia . ^^^ Geldanamycin ( GA ) , an Hsp 90 antagonist , promoted efficient ubiquitination and proteasome mediated degradation of HIF 1 alpha in RCC in both normoxia and hypoxia . ^^^ Hsp 90 antagonists also inhibited HIF 1 alpha transcriptional activity and dramatically reduced both hypoxia induced accumulation of VEGF mRNA and hypoxia dependent angiogenic activity . ^^^ These findings demonstrate that disruption of Hsp 90 function 1 ) promotes HIF 1 alpha degradation via a novel , oxygen independent E 3 ubiquitin ligase and 2 ) diminishes HIF 1 alpha transcriptional activity . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| The additional molecular targets considered here include the type 1 insulin like growth factor receptor , the epidermal growth factor receptor , mammalian target of rapamycin , NF kappaB , hypoxia inducible factor 1alpha , hsp 90 , cyclooxygenase 2 , protein kinase A type 1 , vascular endothelial growth factor , 5 lipoxygenase , 12 lipoxygenase , angiotensin 2 receptor type 1 , bradykinin receptor type 1 , c Src , interleukin 6 , ras , MDM 2 , bcl 2 / bclxL , vitamin D receptor , estrogen receptor beta , and PPAR . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| The specific roles of the ubiquitin mediated proteasome machinery , mitogen activated protein kinase ( MAPK ) and Akt pathways , and heat shock protein 90 ( Hsp 90 ) in the SCH 66336 mediated degradation of hypoxia inducible factor 1alpha ( HIF 1alpha ) were assessed with ubiquitin inhibitors and adenoviral vectors that express constitutively active MAP kinase kinase ( MEK ) 1 , constitutively active Akt , or Hsp 90 . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Hypoxia induced activation of HIF 1 : role of HIF 1alpha Hsp 90 interaction . ^^^ Using a EGFP HIF 1alpha fusion protein , co immunoprecipitation experiments evidenced that the chimeric protein expressed in COS 7 cells interacts with Hsp 90 in normoxia but not in hypoxia . ^^^ We also demonstrated that Hsp 90 interacts with the bHLH PAS domain of HIF 1alpha . ^^^ Moreover , Hsp 90 is not co translocated with HIF 1alpha into the nucleus . ^^^ These results indicate that Hsp 90 is a major regulator in HIF 1alpha activation . . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Depletion of the dephosphorylated HIF 1alpha , by using the Hsp 90 inhibitor geldanamycin A that had little effect on the phosphorylated HIF 1alpha expression , suppressed p 53 induction and subsequent apoptosis . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Inhibition of the ATP dependent chaperone activity of HSP 90 by novobiocin or geldanamycin prevented heat induced as well as hypoxia induced HIF 1alpha accumulation , indicating a common role of the HSP 90 chaperone activity in HIF 1alpha stabilization by these two environmental parameters . . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Our results , the first to show that radicicol specifically inhibits the interaction between the HIF 1alpha / Arnt heterodimer and HRE , suggest that Hsp 90 modulates the conformation of the HIF 1alpha / Arnt heterodimer , making it suitable for interaction with HRE . ^^^ |
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| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| We investigated the effect of apigenin on the interaction of HIF 1alpha with heat shock protein 90 ( Hsp 90 ) , which is reported to be important for the stabilization of HIF 1alpha , and found that VEGF expression was inhibited via degradation of HIF 1alpha through interference with the function of Hsp90 . . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Hsp 90 client proteins important in breast cancer include the estrogen receptor , the serine threonine kinases Raf 1 and Akt , the receptor tyrosine kinase ErbB2 / Neu , and the hypoxia inducible transcription factor HIF 1alpha . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Heat shock protein 90 ( Hsp 90 ) is an essential protein that controls the activity , turnover , and trafficking of a variety of other proteins including HIF 1alpha and cell cycle regulators . ^^^ Hsp 90 inhibitors like geldanamycin therefore have the potential to target tumor cell survival by at least two mechanisms , compromising the accumulation of HIF 1alpha and cell proliferation . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Because heat shock protein 90 ( HSP 90 ) plays an important role in HIF 1alpha protein stabilization and because HSP 90 inhibitors are currently being tested in clinical phase 1 trials for anticancer treatment , we investigated their role as anti HIF 1alpha agents . ^^^ In line with these data , low dose treatment with HSP 90 inhibitors increased and high dose treatment reduced hypoxic HIF 1alpha protein levels , respectively . ^^^ HIF 1alpha protein stabilized by HSP 90 inhibitors localized to the nucleus . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Geldanamycin ( GA ) , a benzoquinone ansamycin , is a naturally occurring inhibitor of heat shock protein ( Hsp 90 ) , which regulates the transcription activity of hypoxia inducible factor 1 ( HIF 1alpha ) . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| The inhibition of proteasome in the growth phase led to HIF 1alpha protein accumulation , whereas in the differentiation phase the inhibition of Hsp 90 , which stabilizes HIF 1alpha , suppressed HIF 1alpha accumulation . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Hsp 90 client proteins include telomerase , mutated p 53 , Bcr Abl , Raf 1 , Akt , HER2 / Neu ( ErbB 2 ) , mutated B Raf , mutated EGF receptor , and HIF 1alpha . ^^^ |
|
| Interacting proteins: Q16665 and P07900 |
Pubmed |
SVM Score :0.0 |
| Inhibition of this pathway by the inactivation of the Hif 1alpha chaperone Hsp 90 in combination with antiangiogenic stress leads to the first reported complete regression of these aggressive breast tumors . . ^^^ |
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