| In the yeast two hybrid system , Mcl 1 binds to the hypophosphorylated mutant of BAD and interacts preferentially with different proapoptotic ( Bax , Bak , Bok , Bik , and BOD ) compared with antiapoptotic Bcl 2 family members ( Bcl 2 , Bcl xL , Bcl w , Bfl 1 , CED 9 , and BHRF 1 ) . ^^^ |
| Using reverse transcriptase polymerase chain reaction , cloning , and sequencing techniques , we have found that HL 60 cells express bak , bik , bax , bad , bcl 2 , bcl xL , bcl w , bfl 1 , fas , and caspases 1 4 and 7 10 . ^^^ After DMSO treatment , bak , bcl w , bfl 1 , fas , and caspases 1 and 9 were up regulated , whereas bik , bcl 2 , and caspases 2 , 3 , and 10 were down regulated at different degrees , achieving mRNA expression levels that correlated with those detected in peripheral blood neutrophils . ^^^ Peripheral blood neutrophils expressed bak , bad , bcl w , bfl 1 , fas , and caspases 1 , 3 , 4 , and 7 10 , but hardly expressed bcl 2 , bcl xL , bik , bax , and caspase 2 . ^^^ |