| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| E2F 5 is more closely related to E2F 4 ( 78 % similarity ) than to E2F 1 ( 57 % similarity ) . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Together with the recently reported E2F 4 , E2F 5 defines a new branch of the E2F family . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| However , it has no effect on the transcription of the E2F 3 , E2F 4 , E2F 5 , DP 1 , DP 2 , or p16 / Ink4 genes . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| E2F 4 , but not E2F 1 , E2F 2 or E2F 5 , activates productive infection with similar efficiency as E1A . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Among the members of the E2F family , E2F1 to E2F4 , but not E2F5 or E2F6 , activated the JPO1 / CDCA7 reporter construct . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Comparison of the sequence with other members of the family indicates that E2F 5 shows a greater level of similarity with E2F 4 than to E2F 1 , 2 and 3 . ^^^ The structural and functional similarity of E2F 5 and E2F 4 defines a subfamily of E2F proteins . . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| E2F 4 and E2F 5 , two members of the E2F family , are expressed in the early phases of the cell cycle . ^^^ Here we describe the characterization of cDNAs encoding two unusual E2Fs , E2F 4 and E2F 5 , each identified by the ability of their gene product to interact with p 130 in a yeast two hybrid system . ^^^ However , in contrast to E2F 1 , E2F 4 and E2F 5 fail to bind pRb in a two hybrid assay . ^^^ Moreover , they show a unique pattern of expression in synchronized human keratinocytes : E2F 4 and E2F 5 mRNA expression is maximal in mid G 1 phase before E2F 1 expression is detectable . ^^^ These findings suggest that E2F 4 and E2F 5 may contribute to the regulation of early G 1 events including the G0 / G1 transition . . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| The p16INK4 induced G 1 arrest was not affected by expression of E2F 4 , E2F 5 , or DP 1 alone , but simulataneous expression of E2F 4 and DP 1 could overcome this block . ^^^ Since E2F 4 and E2F 5 can not promote S phase entry by themselves , our results may provide an explanation for the apparent lack of aberrations in p 107 or p 130 in human cancer . . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Northern blot analysis of the expression of E2F and DP family members showed that the E2F 1 , E2F 4 , and E2F 5 mRNA was growth and senescence dependent , whereas E2F 3 , DP 1 , and DP 2 expression was constitutive and senescence independent . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| We find selective up regulation of E2F 2 , E2F 4 , and E2F 5 transcripts in epidermis and intestinal epithelium at important developmental stages . ^^^ Stratification of the epidermis into basal , proliferating cells and suprabasal , terminally differentiating cells at 15 . 5 19 . 5 days of gestation coincides with expression of E2F 2 and E2F 4 in basal cells and of E2F 5 in suprabasal cells . ^^^ This suggests that E2F 2 and E2F 4 may participate in maintaining epithelial cells in a proliferative , undifferentiated phenotype , whereas E2F 5 may be important to maintain the differentiated state . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis of BIP with cyclins / CDKs and E2F antisera indicated association with cdc 2 , cdk 2 , cdk 4 , cyclin B , cyclin D , cyclin A and E2F 4 but not with cdk 3 , cdk 5 , cdk 6 , E2F 1 , E2F 2 , E2F 3 , E2F 5 and cyclin E . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| E2F 5 protein is more homologous to E2F 4 ( 72 % amino acid identity ) than to E2F 1 , E2F 2 , and E2F 3 ( 35 % amino acid identity ) . ^^^ Based on structural and functional criteria , the E2F family appears to comprise two distinct sub families , one composed of E2F 1 , E2F 2 , and E2F 3 and the other composed of E2F 4 and E2F 5 , E2F 5 mRNA is expressed in a wide variety of human tissues . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| These activities correlate with the appearance of hyperphosphorylated p 130 , an Rb family member important in regulating E2F 4 and E2F 5 activity in fibroblastic cells . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| While DP proteins stabilize DNA binding of E2F proteins , and influence the entry of E2F 4 and E2F 5 into the nucleus , the role of DP proteins in E2F dependent gene expression is not well understood . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis data showed 3 . 0 to 7 . 6 fold upregulation of E2F 1 , E2F 2 , E2F 3 , E2F 4 and E2F 5 in tumors compared to normal epidermis . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| In terminally differentiated L 6 myotubes , E2F 1 , E2F 3 , and E2F 5 were primarily cytoplasmic , E2F 2 was nuclear , whereas E2F 4 became partitioned between both compartments . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| The results show that ( 1 ) at 8 h postinfection or thereafter , E2F 1 and E2F 5 were posttranslationally modified and / or translocated from nucleus to the cytoplasm , ( 2 ) E2F 4 was hyperphophorylated , and ( 3 ) overall , E2F binding to cognate DNA sites was decreased at late times after infection . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| No differences were observed between RA sensitive and RA resistant ovarian carcinoma cells in the levels of expression of many cell cycle genes including cyclin A , B and E , cdk 2 , 4 and 6 , E2F 1 , E2F 2 , E2F 3 , E2F 4 , E2F 5 , DP 1 and DP 2 . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Interleukin 3 ( IL 3 ) dependent proliferation of haematopoietic cells is specifically inhibited by p 130 , a member of the pRB family proteins . p 130 interacts with the cell cycle regulatory E2F transcription factors , notably E2F 4 and E2F 5 , and affects promoters containing E2F binding sites through two distinct mechanisms . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| It has been proposed that E2F 4 and E2F 5 , which lack defined nuclear localization signal ( NLS ) consensus sequences , enter the nucleus in association with NLS containing DP 2 or pRB family proteins . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Genes examined included the retinoblastoma susceptibility gene ( Rb 1 ) ; cyclins D 1 , D 2 , A , and E ; the CDK inhibitors p 18 , p 19 , and p 27 ; CDK 2 and CDK 6 ; transcription factors E2F 4 , E2F 5 , and DP 1 ; and the neurofibromatosis type 2 gene . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| E2F 1 , E2F 2 , and E2F 3 ( but not E2F 4 and E2F 5 ) repressed hTERT promoter activity in human tumor cells , whereas they activated it in normal somatic cells . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| We have isolated several peptides from random peptide phage display libraries that specifically recognize the cell cycle regulatory transcription factor E2F and inhibit DNA binding of E2F / DP heterodimers ( E2F 1 , E2F 2 , E2F 3 , E2F 4 or E2F 5 , and DP 1 ) . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| The promoter activity of the mouse FGFR 2 gene is also positively regulated by E2F 2 and E2F 3 , but not by E2F 4 and E2F 5 . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| For this interaction , the C terminal part of Sp 1 and the N terminus of E2F1 , a domain also present in E2F2 and E2F3 but absent in E2F4 and E2F5 , were essential . ^^^ Accordingly , E2F1 to E2F3 but not E2F4 and E2F5 were found to bind sp 1 in vitro . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| We demonstrated that E2F mediated growth regulation of dhfr transcription requires activation of the dhfr promoter in S phase and that the C terminal activation domains of E2F1 , E2F4 , and E2F5 , when fused to the Gal 4 DNA binding domain , are sufficient to specify position dependent activation . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| We compared the abilities of E2F1 , E2F4 , and E2F5 to activate transcription from a variety of gene promoters and found that in all cases E2F1 was the most potent activator , followed by E2F4 and then by E2F5 . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate that E2F4 is highly upregulated following the neuronal differentiation of PC 12 cells with nerve growth factor ( NGF ) , while E2F1 , E2F3 , and E2F5 are downregulated . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| In contrast , in melanoma cells , free E2F DNA binding activity ( E2F2 and E2F4 , to a lesser extent E2F1 , E2F3 , and occasionally E2F5 ) , was constitutively maintained at high levels independently of external melanocyte mitogens . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| But , unlike the E2F4 and E2F5 proteins , which are also expressed in quiescent cells and form complexes with the p 130 protein , the E2F3b protein associates with Rb and represents the predominant E2F Rb complex in quiescent cells . ^^^ |
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| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| In contrast , the constitutive expression of the E2F3b gene more closely reflects the control of expression of the E2F4 and E2F5 genes . . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| In contrast , methylation of the E2F elements derived from the c myc and c myb promoters minimally affects the binding of E2F2 , E2F3 , E2F4 , and E2F5 but significantly inhibits the binding of E2F1 . ^^^ |
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| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| E2F4 and E2F5 play an essential role in pocket protein mediated G 1 control . ^^^ E2F4 and E2F5 constitute a defined subset of the family . ^^^ Here , we report that simultaneous inactivation of E2F4 and E2F5 in mice results in neonatal lethality , suggesting that they perform overlapping functions during mouse development . ^^^ Thus , E2F4 and E2F5 are dispensable for cell cycle progression but necessary for pocket protein mediated G 1 arrest of cycling cells . . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| These chosen dCSTs include among others cyclins D and E , two cyclin dependent kinases , two other kinases , transcription factors E2F4 , E2F5 , and p 130 , a DNA repair gene , a gene for the signalosome subunit , and potential guanine nucleotide binding factors . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Differences in human and rat FSH receptors promote activity as a result of the transcriptional factors : E2F1 , E2F4 and E2F5 overexpression ] . ^^^ In order to determine the influence of E2F1 , E2F4 and E2F5 , on FSH R promoter activity , cells were transfected either with promoter construct alone or with its mixture with selected expression vector . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Recent work shows that c Myc repression by TGFbeta is mediated by the nuclear translocation of a novel , preformed complex composed of Smad 3 , E2F4 or E2F5 , and the Rb related factor p107 . . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Silibinin treatment of cells resulted in a strong increase ( up to 2 . 3 and 5 . 4 fold ) in the levels of hypophosphorylated Rb / p107 and Rb2 / p130 , respectively , but a strong decrease ( 91 , 78 and 45 % ) in protein levels of transcription factors E2F3 , E2F4 and E2F5 , respectively . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Loss of E2F3 , but not E2F1 , E2F2 , E2F4 , or E2F5 results in unregulated cyclin E dependent kinase activity , defects in nucleophosmin B association with centrosomes , and premature centriole separation and duplication . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| We further demonstrate that LMP 1 also blocks the function of E2F4 and E2F5 ( E2F4 / 5 ) transcription factors through promoting their nuclear export in a CRM 1 dependent manner . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| The sequence of the E2F site was noncanonical and its interaction with E2F protein was confirmed by a competitive EMSA using a consensus E2F oligonucleotide probe and by demonstrating a supershift with the antibody against the E2F4 and E2F5 pocket protein , p 107 . 5 ' RACE carried out with CWR22Rv1 and HepG 2 cells detected a single transcription start site ( tsp ) distal to the basal promoter and identified two new MRP 7 transcripts with very short 5 ' UTR sequences compared to transcripts found by others in nontumorous human tissue . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Downstream in CDKI CDK cyclin cascade , consistent with its inhibitory effect on CDK kinase activity , IP 6 treatment of cells increased hypophosphorylated levels of retinoblastoma ( Rb ) with a decrease in Rb phosphorylation at serine 780 , 807 , and 811 sites , and caused a moderate to strong decrease in the levels of transcription factors E2F1 , E2F4 , and E2F5 . ^^^ |
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| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Furthermore , directed expression of E2F1 , E2F3 , and E2F5 led to a transcriptional activation of several proapoptotic genes , which were mitigated by E2F2 and E2F4 . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Based on current evidence in the field , mammalian E2Fs can be functionally categorized into either transcriptional activators ( E2F1 , E2F2 , and E2F3a ) or repressors ( E2F3b , E2F4 , E2F5 , E2F6 , and E2F7 ) . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Although E2Fs are known to be critical regulators of the cell cycle , it is still unclear whether family members E2F3a , E2F4 or E2F5 are individually capable of inducing cell cycle entry in vivo . ^^^ METHODS : Transgenic mice were generated by microinjection of constructs that contained E2F cDNAs ( E2F3a , E2F4 or E2F5 ) linked to the mouse lens specific alphaA crystallin promoter . ^^^ CONCLUSIONS : E2F3a and E2F4 , but not E2F5 , function to induce cell cycle entry , although E2F4 has more modest activity . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| The E2F4 and E2F5 proteins specifically associate with the Rb related p 130 protein in quiescent cells to repress transcription of various genes encoding proteins important for cell growth . ^^^ |
|
| Interacting proteins: Q15329 and Q16254 |
Pubmed |
SVM Score :0.0 |
| Experimental Design and RESULTS : We now show that E2F1 and E2F4 , but not E2F2 , E2F3 , or E2F5 , bind to the ARHI promoter and repress its activity in ovarian cancer cells . ^^^ |
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