Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
E2F 4 and 5 share common sequences with E2F 1 , E2F 2 , and E2F 3 and , like these other E2Fs , the ability to heterodimerize with DP 1 , thereby acquiring the ability to bind an E2F DNA recognition sequence with high affinity . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Like the other E2Fs , E2F 4 requires DP 1 for efficient DNA binding and transcriptional activation of E2F site containing promoters . ^^^ Increased expression of E2F 4 and DP 1 in SaoS 2 osteosarcoma cells causes a shift from G 1 phase cells to S and G2 / M phase cells , suggesting a role for E2F 4 in regulation of cell cycle progression . ^^^ We show that expression of E2F 4 and DP 1 together with an activated ras oncogene in rat embryo fibroblasts , causes transformation , indicating that E2F 4 has oncogenic activity . . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
The p16INK4 induced G 1 arrest was not affected by expression of E2F 4 , E2F 5 , or DP 1 alone , but simulataneous expression of E2F 4 and DP 1 could overcome this block . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Here , we show that the B myb repressor element is specifically recognised by heterodimers consisting of DP 1 and E2F 1 , E2F 3 or E2F 4 . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
The level of p 107 protein increases during induction of differentiation ; there is no change in the level of cdk 2 protein and E2F 4 and DP 1 proteins during the first 4 days . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
We report here , however , that transfection of cells with the known components of this complex , p 107 , E2F 4 and DP 1 , did not repress the B myb promoter in cycling NIH3T3 cells , although p 107 inhibited transcription transactivation by E2F 4 / DP 1 . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Northern blot analysis of the expression of E2F and DP family members showed that the E2F 1 , E2F 4 , and E2F 5 mRNA was growth and senescence dependent , whereas E2F 3 , DP 1 , and DP 2 expression was constitutive and senescence independent . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
In extracts from log phase cells , E2F complexes contained predominantly E2F 4 and E2F 2 in association with DP 1 , and DNA binding assays showed complexes containing E2F 2 preferentially interact with only one of the two overlapping E2F sites . ^^^ DP 1 complexes in late G 1 , one of which contained E2F 4 and a second which contained an unidentified E2F . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
The predominant E2F complex in human primary haemopoietic cells and in AML blasts contains E2F 4 , DP 1 and p 130 . ^^^ On electromobility shift assays ( EMSA ) a single E2F DNA binding complex was detected in T cells , B cells and monocytes which was shown to contain E2F 4 , DP 1 and p 130 , indicating that all quiescent haemopoietic cells have the same complex . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Down regulation of E2F 1 , a subunit of E2F , was observed after 8 h of culture with IFN alpha ; expression of E2F 4 , another subunit of E2F , and DP 1 , a heterodimeric partner of E2F , was unaffected . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Cotransfection of expression vectors encoding p 107 , p 130 , or DP 2 , but not DP 1 , resulted in the nuclear localization of E2F 4 and 5 . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Support for this is provided by showing that the principal E2F complex consists of hypophosphorylated p 130 , E2F 4 , and DP 1 . ^^^ As cells enter G 1 , E2F 4 is dephosphorylated to several hypophosphorylated forms and three new DNA binding complexes appear , including one containing E2F 4 , DP 1 , and p 107 . ^^^ We suggest that mobilized CD34+ cells may be maintained in G 0 by p 130 , E2F 4 , and DP 1 and the coordinate dephosphorylation of E2F 4 and hyperphosphorylation of p 130 may be central to the initiation of proliferation . . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Inhibition of E2F 4 / DP 1 stimulated transcription by p 202 . ^^^ Here we report that expression of p 202 inhibited E2F 4 / DP 1 stimulated transcription of a reporter gene in transfected cells . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
In these cells , E2F 3 , E2F 4 , and DP 1 are regulated by both IL 3 and granulocyte colony stimulating factor ( G CSF ) , whereas E2F 1 was expressed at low levels and was not regulated by either cytokine . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
However , it has no effect on the transcription of the E2F 3 , E2F 4 , E2F 5 , DP 1 , DP 2 , or p16 / Ink4 genes . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
One of the infection specific bands contained the proteins E2F 4 , DP 1 , and p 130 , which were maintained in the infected cells as uniquely phosphorylated species . ^^^ These results suggest that an altered E2F 4 DP 1 p 130 complex along with viral early gene expression may play a role in the transcriptional regulation of cyclin E mRNA during HCMV infection . . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Using tetracycline responsive promoters , here we compared the effects of ectopic expression of E2F 1 , DP 1 and E2F 4 on cell cycle progression and apoptosis in Chinese hamster cell lines . ^^^ We found that E2F 4 , as well as DP 1 and E2F 1 , induced growth arrest and caspase dependent apoptosis . ^^^ E2F 4 did not have a marked effect on cell cycle progression , while E2F 1 induced DNA synthesis of resting cells and DP 1 arrested cells in G 1 . ^^^ Our results suggest that expression of E2F 4 at elevated levels induces growth arrest and apoptosis of mammalian cells through a mechanism distinct from E2F 1 and DP 1 . . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
No differences were observed between RA sensitive and RA resistant ovarian carcinoma cells in the levels of expression of many cell cycle genes including cyclin A , B and E , cdk 2 , 4 and 6 , E2F 1 , E2F 2 , E2F 3 , E2F 4 , E2F 5 , DP 1 and DP 2 . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Genes examined included the retinoblastoma susceptibility gene ( Rb 1 ) ; cyclins D 1 , D 2 , A , and E ; the CDK inhibitors p 18 , p 19 , and p 27 ; CDK 2 and CDK 6 ; transcription factors E2F 4 , E2F 5 , and DP 1 ; and the neurofibromatosis type 2 gene . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
We have isolated several peptides from random peptide phage display libraries that specifically recognize the cell cycle regulatory transcription factor E2F and inhibit DNA binding of E2F / DP heterodimers ( E2F 1 , E2F 2 , E2F 3 , E2F 4 or E2F 5 , and DP 1 ) . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Thus , the mechanisms of decreased expression of cyclin A in the presence of dnDP 1 seem to involve inactivation of DP 1 complexes containing E2F 3 and E2F 4 . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Recombinant human E2F1 , E2F4 , and DP 1 fusion proteins were affinity purified from bacteria expressing these genes . ^^^ A combination of either E2F1 or E2F4 with their dimerization partner , DP 1 , gave preparations that exhibited binding to the E2F site containing DNA fragment . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Consistent with previous data from human and mouse fibroblasts and T lymphocytes , E2F4 and DP 1 form the predominant E2F heterodimers both in G 0 and G 1 phases of the human B lymphocyte cell cycle , whereas E2F1 and 3 are first detected in late G 1 , and their expression levels increase towards S phase . ^^^ Intriguingly , the major E2F complex that we detected in quiescent human B lymphocytes is consisted of pRB , E2F4 , and DP 1 . ^^^ We also observed an increase in electrophoretic mobility of the predominant E2F components , DP 1 and E2F4 , as B lymphocytes progressed from G 0 into early G 1 . ^^^ This modulation of DNA binding activity mediated through the dephosphorylation of DP 1 and E2F4 could help to explain the lack of in vivo DNA footprinting in late G 1 and S phases of gene promoters negatively regulated through E2F sites and suggests a novel mechanism for controlling E2F transcriptional activity during the transition from quiescence to proliferation . . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
When DP 1 was connected to cyclin A Cdk 2 through E2F4 and p 107 , its phosphorylation was very inefficient , although its association with cyclin A Cdk 2 was stable . ^^^ In contrast , DP 1 was efficiently phosphorylated when weakly connected to cyclin A Cdk 2 via E2F1 or E2F4 with a fused cyclin A binding domain of E2F1 . ^^^ The transactivation activity of E2F4 DP 1 heterodimers was reduced when DP 1 was phosphorylated , while a phosphorylation deficient mutant of DP 1 resisted this down regulation . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Western blot analyses of whole cell extracts revealed that amounts of E2F4 , E2F1 , DP 1 , and p 107 remained unchanged after infection of C 33 cells . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
We find that E2F4 and DP 1 form the predominant E2F heterodimers in the G 0 and G 1 phases of the cell cycle , complexed with hypophosphorylated p 130 . ^^^ We also observed an increase in electrophoretic mobility of DP 1 and E2F4 as B cells progressed from G 0 into early G 1 , resulting from their dephosphorylation . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Coexpression of DP 1 with E2F1 or E2F4 in the epidermis of bigenic mice modestly enhanced proliferation and apoptosis over the levels induced by E2F1 or E2F4 expression alone . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
We found that the predominant E2F complex bound to the F type promoter in unstimulated / quiescent cells contains E2F4 , DP 1 and p 130 proteins . ^^^ Further supporting this idea , we obtained results showing that treatment of cycling NIH 3T3 cells with either wortmannin or LY 294002 induces the accumulation of the transcriptionally repressive p 130 E2F4 DP 1 complex . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
Here we investigated the role of E2F1 and E2F4 expression , with or without co expression of DP 1 or DP 2 , on cell proliferation in transiently transfected primary rat MCs . ^^^
Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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Interacting proteins: Q16254 and Q14186	Pubmed	SVM Score :0.0
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