| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.67001066 |
| Mutations in NCS 1 equivalent to those that abolish the interaction of recoverin , another EF hand containing Ca ( 2+ ) sensor , with its downstream target rhodopsin kinase , lost their ability to enhance exocytosis . 0.67001066^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recent studies indicate that recoverin inhibits rhodopsin phosphorylation by directly regulating rhodopsin kinase , a G protein coupled receptor kinase ( GRK ) . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Inhibition of rhodopsin kinase by recoverin . ^^^ Ca ( 2+ ) recoverin directly inhibits rhodopsin kinase activity , and Ca ( 2+ ) dependent binding of recoverin to rod outer segment membranes is not required for its action . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Ca ( 2+ ) dependent interaction of recoverin with rhodopsin kinase . ^^^ When recoverin was added , the activity of RK became sensitive to free Ca2+ , with EC 50 = 3 microM . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Calcium sensitive control of rhodopsin phosphorylation in the reconstituted system consisting of photoreceptor membranes , rhodopsin kinase and recoverin . ^^^ Rhodopsin phosphorylation in the reconstituted system consisting of urea washed photoreceptor membranes , rhodopsin kinase and recoverin is regulated by Ca2+ : the process takes place at low [ Ca2+ ] but is suppressed at high [ Ca2+ ] . ^^^ In the absence of recoverin , rhodopsin kinase is active irrespective of the cation concentration used . ^^^ Hence , recoverin is an inhibitor ( at high [ Ca2+ ] ) but not an activator of rhodopsin kinase . ^^^ Based jointly on these data obtained on the reconstituted system and on our preceding experiments on rod outer segments suspension , one may conclude that ( 1 ) the function of recoverin in retina rod cells is the Ca ( 2+ ) sensitive control of rhodopsin phosphorylation and ( 2 ) the presence of recoverin is essential and sufficient to provide rhodopsin kinase with the Ca2+ sensitivity . . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| We conclude that the Ca2+ effects observed are mediated by recoverin which inhibits rhodopsin kinase at a high Ca2+ level . . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| N myristoylation of recoverin enhances its efficiency as an inhibitor of rhodopsin kinase . ^^^ Recoverin , a recently identified member of the EF hand superfamily of Ca ( 2+ ) binding proteins , is capable to inhibit rhodopsin phosphorylation by rhodopsin kinase at high but not at low free [ Ca2+ ] . ^^^ To clarify the role of the N terminal acylation of recoverin in its inhibitory action upon rhodopsin phosphorylation , we compared the efficiency of myristoylated and non myristoylated forms of recombinant recoverin as inhibitors of rhodopsin kinase activity . ^^^ We have found that rhodopsin phosphorylation by purified rhodopsin kinase , which does not depend on free [ Ca2+ ] in the absence of recoverin , is regulated by Ca2+ in the presence of both forms of the recombinant protein . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Calcium bound recoverin targets rhodopsin kinase to membranes to inhibit rhodopsin phosphorylation . ^^^ We found that Ca2+ bound recoverin forms a complex with rhodopsin kinase preferentially at the membrane surface . ^^^ Addition of increasing amounts of membranes promoted the membrane association of recoverin , and remarkably suppressed rhodopsin kinase activity . ^^^ It was concluded that the Ca2+ recoverin rhodopsin kinase complex is stabilized by membrane association , leading to effective suppression of the kinase activity . . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| These include rhodopsin phosphorylation and subsequent binding of arrestin ; modulation of rhodopsin kinase by S modulin ( recoverin ) ; regulation of G protein and phosphodiesterase inactivation by GTPase activating factors ; and modulation of guanylyl cyclase by a high affinity Ca ( 2+ ) binding protein . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| In particular , it was found that retinal rhodopsin kinase ( GRK 1 ) , but not the ubiquitous betaARK 1 ( GRK 2 ) , can be inhibited by the photoreceptor specific Ca2+ binding protein recoverin through direct binding . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Using a reconstructed system , containing urea washed rod outer segment membranes , purified rhodopsin kinase ( RK ) , and a recoverin , it was shown that the three recoverin forms ( natural , recombinant nonmyristoylated , and recombinant myristoylated ones ) perform the calcium dependent regulation of the activity of RK with half a maximum effect at a free calcium concentration of 2 microM . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin inhibits the phosphorylation of dark adapted rhodopsin more than it does that of bleached rhodopsin : a possible mechanism through which rhodopsin kinase is prevented from participation in a side reaction . ^^^ Recoverin and Ca2+ dependent inhibition of rhodopsin kinase was found to be inversely related to the extent of bleaching ; the lower the fraction of rhodopsin bleached , the greater the inhibition . ^^^ These results are interpreted to suggest that one of the roles of the recoverin dependent regulation of the activity of rhodopsin kinase is to prevent the enzyme from participating in the unwanted phosphorylation of dark adapted rhodopsin , directing it to fulfil its ' correct ' function of quenching the transduction activity of Rho * . . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin is a calcium binding protein that regulates the vertebrate photoresponse by inhibiting rhodopsin kinase in response to high calcium concentrations . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin , a calcium binding protein , is supposed to have rhodopsin kinase as a target in the retinal rod cell . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Some involve calcium binding proteins like recoverin , guanylyl cyclase activating protein and calmodulin , and their target proteins rhodopsin kinase , guanylyl cyclase , the cGMP gated channel , and NO synthase . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| One such protein is recoverin , a calcium sensor in retinal rod cells , which controls the lifetime of photoexcited rhodopsin by inhibiting rhodopsin kinase . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Autophosphorylation and ADP regulate the Ca2+ dependent interaction of recoverin with rhodopsin kinase . ^^^ Recoverin is a 23 kDa myristoylated Ca2+ binding protein that inhibits rhodopsin kinase . ^^^ We have used surface plasmon resonance to investigate the influences of Ca2+ , myristoylation , and adenine nucleotides on the recoverin rhodopsin kinase interaction . ^^^ Finally , our studies also revealed two separate and previously unreported effects of adenine nucleotides on the recoverin rhodopsin kinase binding . ^^^ Our analyses confirmed that Ca2+ is required for recoverin to bind RK . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| While GRK 1 ( rhodopsin kinase ) is inhibited by the photoreceptor specific Ca2+ binding protein recoverin , other GRKs can be inhibited by Ca2+ calmodulin . ^^^ In contrast , the unique N terminally localized GRK 1 site for recoverin had no clearly defined structural characteristics . ^^^ Interestingly , while the recoverin and calmodulin binding sites in GRK 1 do not overlap , recoverin GRK 1 interaction is inhibited by calmodulin , most likely via an allosteric mechanism . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| At least three proteins appear to play a role : rhodopsin kinase , arrestin and recoverin . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| One such protein , recoverin , has been proposed to regulate RK activity contributing to adaptation to background illumination in retinal photoreceptor cells . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Several EF hand recoverin mutants were obtained and their abilities to bind to photoreceptor membranes and to inhibit rhodopsin kinase were determined . ^^^ The latter mutant inhibited rhodopsin kinase even more effectively than the wild type recoverin , containing two working Ca2+ binding structures . . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| In the presence of calcium , rhodopsin kinase ( GRK 1 ) is inhibited by the photoreceptor specific CSP recoverin through direct binding . ^^^ Several other recoverin homologues ( including NCS 1 , VILIP 1 , and hippocalcin ) are also able to inhibit GRK 1 in a calcium dependent manner . ^^^ This mechanism , however , is highly selective with respect to the GRK subtypes : GRK 1 , but not GRK 2 and GRK 5 , is regulated by recoverin and other NCS , but GRK 4 , 5 , and 6 , which belong to the GRK 4 subfamily are potently inhibited by CaM , which has little or no effect on members of other GRK subfamilies . ^^^ Calcium dependent inhibition of rhodopsin kinase by recoverin represents one of the mechanisms that control adaptation to light . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin is a calcium binding protein which inhibits rhodopsin kinase from phosphorylating rhodopsin . ^^^ Because cells in the inner layers of the retina do not contain rhodopsin kinase , arrestin , or other phototransduction proteins , it seems likely that recoverin has a different function in those cell types . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Analysis by SDS PAGE of ROS proteins and by western blot using antibodies against opsin , rhodopsin kinase ( RK ) , recoverin , or arrestin demonstrated that a 20 kDa protein and RK were selectively less abundant in RCS than in control rats . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| S modulin , found in frog rods , or its bovine homologue recoverin , is a 23 kDa Ca ( 2+ ) binding protein that inhibits rhodopsin phosphorylation at high Ca2+ concentrations by inhibiting rhodopsin kinase . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Purification of rhodopsin kinase by recoverin affinity chromatography . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin is a 23 kDa heterogeneously myristoylated Ca ( 2+ ) binding protein that inhibits rhodopsin kinase . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| In the presence of calcium , rhodopsin kinase ( GRK 1 ) is inhibited by the photoreceptor specific CSP recoverin through direct binding . ^^^ Several other recoverin homologues ( including NCS 1 , VILIP 1 and hippocalcin ) are also able to inhibit GRK 1 . ^^^ This mechanism is , however , highly selective with respect to the GRK subtypes : while GRK 1 , but not GRK 2 and GRK 5 , is regulated by recoverin and other NCS , GRK 4 , 5 and 6 , that belong to the GRK 4 subfamily , are potently inhibited by CaM , which had little or no effect on members of other GRK subfamilies . . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| By RT PCR and DNA sequencing we identified the expression of rhodopsin and components linked to its deactivation , including rhodopsin kinase , recoverin , and visual arrestin . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin is an EF hand Ca ( 2+ ) binding protein that is suggested to control the activity of the G protein coupled receptor kinase GRK 1 or rhodopsin kinase in a Ca ( 2+ ) dependent manner . ^^^ We discuss this mechanism as one critical step for inhibition of rhodopsin kinase by recoverin . . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Ca2+ dependent control of rhodopsin phosphorylation : recoverin and rhodopsin kinase . ^^^ The leading candidate for the role of an intracellular target for recoverin is believed to be rhodopsin kinase , a member of a family of G protein coupled receptor kinases . ^^^ The present review considers recoverin , rhodopsin kinase and their interrelationships in the in vitro as well as in vivo contexts . . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin and rhodopsin kinase . ^^^ Recoverin and rhodopsin kinase are expressed primarily in retinal photoreceptors and they interact with each other in a Ca2+ dependent manner . ^^^ Recoverin can be viewed as a negative regulator of rhodopsin kinase in vertebrate phototransduction . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin is an N myristoylated 23 kDa calcium binding protein from retina , which modulates the Ca2+ sensitive deactivation of rhodopsin via Ca2+ dependent inhibition of rhodopsin kinase . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : Following photoactivation of rhodopsin , recoverin inhibits rhodopsin kinase activity in retinal photoreceptors by reducing the binding of arrestin to rhodopsin and therefore prolonging the termination of the phototransduction cascade . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Impairment of Ca ( 2+ ) binding in recoverin leads to a disturbance of the Ca ( 2+ ) myristoyl switch and loss of its regulatory properties , i . e . inhibiton of rhodopsin kinase . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the drug antagonized the inhibitory action of recoverin in a rhodopsin kinase assay with IC 50 values of 400 microM . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| In the present study , we examined the expression of photoreceptor specific proteins in the pineal gland ( PG ) including rhodopsin kinase ( RK ) , arrestin and recoverin , which are known to be commonly present in both photoreceptor and PG , in order to elucidate the pathological relationship between retina and PG during retinal degeneration . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin and rhodopsin kinase activity in detergent resistant membrane rafts from rod outer segments . ^^^ Here we report the presence of rhodopsin kinase and recoverin in DRMs that were isolated in either light or dark conditions at high and low Ca2+ concentrations . ^^^ Inhibition of rhodopsin kinase activity by recoverin was more effective in DRMs than in the initial rod outer segment membranes . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| One of the Ca2+ binding sites of recoverin exclusively controls interaction with rhodopsin kinase . ^^^ Recoverin is a neuronal calcium sensor protein that controls the activity of rhodopsin kinase in a Ca ( 2+ ) dependent manner . ^^^ Likewise , an interaction surface for the recoverin target rhodopsin kinase is constituted upon Ca2+ binding to the non acylated mutant . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| To establish changes in phototransduction in diabetes , the effects of high glucose on rhodopsin kinase ( RK ) and transducin ( G ( t ) ) , as well as recoverin , were examined in the retina of STZ induced diabetic rats . ^^^ In the diabetic retina , while the expression of RK protein increased , that of G ( talpha ) and recoverin proteins decreased . ^^^ This study illustrates the alterations in RK , G ( talpha ) and recoverin in the diabetic retina that may induce dysfunctions in phototransduction even in early stage diabetes . . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| We suggest that recoverin translocation is adaptive because it may reduce the inhibitory constraint that recoverin imposes on rhodopsin kinase , an enzyme responsible for quenching the photo excited rhodopsin during the photoresponse . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Misexpression of Crx induced adult rat iris cells to express several photoreceptor specific antigens and transcripts , such as rhodopsin , recoverin , cGMP gated channel , arrestin , interphotoreceptor retinal binding protein , rhodopsin kinase , and NeuroD . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| A dark adapted flash series , and stimulus paradigms from the literature eliciting various degrees of light adaptation ( LA ) , were simulated using a full differential equation version of the model that included the addition of Ca2+ feedback onto rhodopsin kinase via recoverin . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase , inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase . ^^^ Recoverin is a calcium dependent inhibitor of rhodopsin kinase . ^^^ This calcium depletion causes release of recoverin from rhodopsin kinase , freeing the kinase to phosphorylate rhodopsin and to terminate the light response . ^^^ Previous studies have shown that recoverin is able to bind to a region at the N terminus of rhodopsin kinase . ^^^ |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35243 and Q15835 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.67001066 |
| Mutations in NCS 1 equivalent to those that abolish the interaction of recoverin , another EF hand containing Ca ( 2+ ) sensor , with its downstream target rhodopsin kinase , lost their ability to enhance exocytosis . 0.67001066^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recent studies indicate that recoverin inhibits rhodopsin phosphorylation by directly regulating rhodopsin kinase , a G protein coupled receptor kinase ( GRK ) . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Inhibition of rhodopsin kinase by recoverin . ^^^ Ca ( 2+ ) recoverin directly inhibits rhodopsin kinase activity , and Ca ( 2+ ) dependent binding of recoverin to rod outer segment membranes is not required for its action . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Ca ( 2+ ) dependent interaction of recoverin with rhodopsin kinase . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Calcium sensitive control of rhodopsin phosphorylation in the reconstituted system consisting of photoreceptor membranes , rhodopsin kinase and recoverin . ^^^ Rhodopsin phosphorylation in the reconstituted system consisting of urea washed photoreceptor membranes , rhodopsin kinase and recoverin is regulated by Ca2+ : the process takes place at low [ Ca2+ ] but is suppressed at high [ Ca2+ ] . ^^^ In the absence of recoverin , rhodopsin kinase is active irrespective of the cation concentration used . ^^^ Hence , recoverin is an inhibitor ( at high [ Ca2+ ] ) but not an activator of rhodopsin kinase . ^^^ Based jointly on these data obtained on the reconstituted system and on our preceding experiments on rod outer segments suspension , one may conclude that ( 1 ) the function of recoverin in retina rod cells is the Ca ( 2+ ) sensitive control of rhodopsin phosphorylation and ( 2 ) the presence of recoverin is essential and sufficient to provide rhodopsin kinase with the Ca2+ sensitivity . . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| We conclude that the Ca2+ effects observed are mediated by recoverin which inhibits rhodopsin kinase at a high Ca2+ level . . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| These include rhodopsin phosphorylation and subsequent binding of arrestin ; modulation of rhodopsin kinase by S modulin ( recoverin ) ; regulation of G protein and phosphodiesterase inactivation by GTPase activating factors ; and modulation of guanylyl cyclase by a high affinity Ca ( 2+ ) binding protein . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| In particular , it was found that retinal rhodopsin kinase ( GRK 1 ) , but not the ubiquitous betaARK 1 ( GRK 2 ) , can be inhibited by the photoreceptor specific Ca2+ binding protein recoverin through direct binding . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Using a reconstructed system , containing urea washed rod outer segment membranes , purified rhodopsin kinase ( RK ) , and a recoverin , it was shown that the three recoverin forms ( natural , recombinant nonmyristoylated , and recombinant myristoylated ones ) perform the calcium dependent regulation of the activity of RK with half a maximum effect at a free calcium concentration of 2 microM . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| N myristoylation of recoverin enhances its efficiency as an inhibitor of rhodopsin kinase . ^^^ Recoverin , a recently identified member of the EF hand superfamily of Ca ( 2+ ) binding proteins , is capable to inhibit rhodopsin phosphorylation by rhodopsin kinase at high but not at low free [ Ca2+ ] . ^^^ To clarify the role of the N terminal acylation of recoverin in its inhibitory action upon rhodopsin phosphorylation , we compared the efficiency of myristoylated and non myristoylated forms of recombinant recoverin as inhibitors of rhodopsin kinase activity . ^^^ We have found that rhodopsin phosphorylation by purified rhodopsin kinase , which does not depend on free [ Ca2+ ] in the absence of recoverin , is regulated by Ca2+ in the presence of both forms of the recombinant protein . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Some involve calcium binding proteins like recoverin , guanylyl cyclase activating protein and calmodulin , and their target proteins rhodopsin kinase , guanylyl cyclase , the cGMP gated channel , and NO synthase . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Calcium bound recoverin targets rhodopsin kinase to membranes to inhibit rhodopsin phosphorylation . ^^^ We found that Ca2+ bound recoverin forms a complex with rhodopsin kinase preferentially at the membrane surface . ^^^ Addition of increasing amounts of membranes promoted the membrane association of recoverin , and remarkably suppressed rhodopsin kinase activity . ^^^ It was concluded that the Ca2+ recoverin rhodopsin kinase complex is stabilized by membrane association , leading to effective suppression of the kinase activity . . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| At least three proteins appear to play a role : rhodopsin kinase , arrestin and recoverin . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Several EF hand recoverin mutants were obtained and their abilities to bind to photoreceptor membranes and to inhibit rhodopsin kinase were determined . ^^^ The latter mutant inhibited rhodopsin kinase even more effectively than the wild type recoverin , containing two working Ca2+ binding structures . . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| In the presence of calcium , rhodopsin kinase ( GRK 1 ) is inhibited by the photoreceptor specific CSP recoverin through direct binding . ^^^ Calcium dependent inhibition of rhodopsin kinase by recoverin represents one of the mechanisms that control adaptation to light . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin inhibits the phosphorylation of dark adapted rhodopsin more than it does that of bleached rhodopsin : a possible mechanism through which rhodopsin kinase is prevented from participation in a side reaction . ^^^ Recoverin and Ca2+ dependent inhibition of rhodopsin kinase was found to be inversely related to the extent of bleaching ; the lower the fraction of rhodopsin bleached , the greater the inhibition . ^^^ These results are interpreted to suggest that one of the roles of the recoverin dependent regulation of the activity of rhodopsin kinase is to prevent the enzyme from participating in the unwanted phosphorylation of dark adapted rhodopsin , directing it to fulfil its ' correct ' function of quenching the transduction activity of Rho * . . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin is a calcium binding protein that regulates the vertebrate photoresponse by inhibiting rhodopsin kinase in response to high calcium concentrations . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin , a calcium binding protein , is supposed to have rhodopsin kinase as a target in the retinal rod cell . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| One such protein is recoverin , a calcium sensor in retinal rod cells , which controls the lifetime of photoexcited rhodopsin by inhibiting rhodopsin kinase . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Autophosphorylation and ADP regulate the Ca2+ dependent interaction of recoverin with rhodopsin kinase . ^^^ Recoverin is a 23 kDa myristoylated Ca2+ binding protein that inhibits rhodopsin kinase . ^^^ We have used surface plasmon resonance to investigate the influences of Ca2+ , myristoylation , and adenine nucleotides on the recoverin rhodopsin kinase interaction . ^^^ Finally , our studies also revealed two separate and previously unreported effects of adenine nucleotides on the recoverin rhodopsin kinase binding . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| While GRK 1 ( rhodopsin kinase ) is inhibited by the photoreceptor specific Ca2+ binding protein recoverin , other GRKs can be inhibited by Ca2+ calmodulin . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin is a calcium binding protein which inhibits rhodopsin kinase from phosphorylating rhodopsin . ^^^ Because cells in the inner layers of the retina do not contain rhodopsin kinase , arrestin , or other phototransduction proteins , it seems likely that recoverin has a different function in those cell types . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Analysis by SDS PAGE of ROS proteins and by western blot using antibodies against opsin , rhodopsin kinase ( RK ) , recoverin , or arrestin demonstrated that a 20 kDa protein and RK were selectively less abundant in RCS than in control rats . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| S modulin , found in frog rods , or its bovine homologue recoverin , is a 23 kDa Ca ( 2+ ) binding protein that inhibits rhodopsin phosphorylation at high Ca2+ concentrations by inhibiting rhodopsin kinase . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Purification of rhodopsin kinase by recoverin affinity chromatography . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin is a 23 kDa heterogeneously myristoylated Ca ( 2+ ) binding protein that inhibits rhodopsin kinase . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| In the presence of calcium , rhodopsin kinase ( GRK 1 ) is inhibited by the photoreceptor specific CSP recoverin through direct binding . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| By RT PCR and DNA sequencing we identified the expression of rhodopsin and components linked to its deactivation , including rhodopsin kinase , recoverin , and visual arrestin . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin is an EF hand Ca ( 2+ ) binding protein that is suggested to control the activity of the G protein coupled receptor kinase GRK 1 or rhodopsin kinase in a Ca ( 2+ ) dependent manner . ^^^ We discuss this mechanism as one critical step for inhibition of rhodopsin kinase by recoverin . . ^^^ |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Ca2+ dependent control of rhodopsin phosphorylation : recoverin and rhodopsin kinase . ^^^ The leading candidate for the role of an intracellular target for recoverin is believed to be rhodopsin kinase , a member of a family of G protein coupled receptor kinases . ^^^ The present review considers recoverin , rhodopsin kinase and their interrelationships in the in vitro as well as in vivo contexts . . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin and rhodopsin kinase . ^^^ Recoverin and rhodopsin kinase are expressed primarily in retinal photoreceptors and they interact with each other in a Ca2+ dependent manner . ^^^ Recoverin can be viewed as a negative regulator of rhodopsin kinase in vertebrate phototransduction . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin is an N myristoylated 23 kDa calcium binding protein from retina , which modulates the Ca2+ sensitive deactivation of rhodopsin via Ca2+ dependent inhibition of rhodopsin kinase . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : Following photoactivation of rhodopsin , recoverin inhibits rhodopsin kinase activity in retinal photoreceptors by reducing the binding of arrestin to rhodopsin and therefore prolonging the termination of the phototransduction cascade . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Impairment of Ca ( 2+ ) binding in recoverin leads to a disturbance of the Ca ( 2+ ) myristoyl switch and loss of its regulatory properties , i . e . inhibiton of rhodopsin kinase . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the drug antagonized the inhibitory action of recoverin in a rhodopsin kinase assay with IC 50 values of 400 microM . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| In the present study , we examined the expression of photoreceptor specific proteins in the pineal gland ( PG ) including rhodopsin kinase ( RK ) , arrestin and recoverin , which are known to be commonly present in both photoreceptor and PG , in order to elucidate the pathological relationship between retina and PG during retinal degeneration . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin and rhodopsin kinase activity in detergent resistant membrane rafts from rod outer segments . ^^^ Here we report the presence of rhodopsin kinase and recoverin in DRMs that were isolated in either light or dark conditions at high and low Ca2+ concentrations . ^^^ Inhibition of rhodopsin kinase activity by recoverin was more effective in DRMs than in the initial rod outer segment membranes . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| One of the Ca2+ binding sites of recoverin exclusively controls interaction with rhodopsin kinase . ^^^ Recoverin is a neuronal calcium sensor protein that controls the activity of rhodopsin kinase in a Ca ( 2+ ) dependent manner . ^^^ Likewise , an interaction surface for the recoverin target rhodopsin kinase is constituted upon Ca2+ binding to the non acylated mutant . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| To establish changes in phototransduction in diabetes , the effects of high glucose on rhodopsin kinase ( RK ) and transducin ( G ( t ) ) , as well as recoverin , were examined in the retina of STZ induced diabetic rats . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| We suggest that recoverin translocation is adaptive because it may reduce the inhibitory constraint that recoverin imposes on rhodopsin kinase , an enzyme responsible for quenching the photo excited rhodopsin during the photoresponse . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Misexpression of Crx induced adult rat iris cells to express several photoreceptor specific antigens and transcripts , such as rhodopsin , recoverin , cGMP gated channel , arrestin , interphotoreceptor retinal binding protein , rhodopsin kinase , and NeuroD . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| A dark adapted flash series , and stimulus paradigms from the literature eliciting various degrees of light adaptation ( LA ) , were simulated using a full differential equation version of the model that included the addition of Ca2+ feedback onto rhodopsin kinase via recoverin . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase , inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase . ^^^ Recoverin is a calcium dependent inhibitor of rhodopsin kinase . ^^^ This calcium depletion causes release of recoverin from rhodopsin kinase , freeing the kinase to phosphorylate rhodopsin and to terminate the light response . ^^^ Previous studies have shown that recoverin is able to bind to a region at the N terminus of rhodopsin kinase . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| One such protein , recoverin , has been proposed to regulate RK activity contributing to adaptation to background illumination in retinal photoreceptor cells . ^^^ |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15835 and P35243 |
Pubmed |
SVM Score :0.0 |
| NA |
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