| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| We recently demonstrated that the LKB 1 tumour suppressor kinase , in complex with the pseudokinase STRAD and the scaffolding protein MO 25 , phosphorylates and activates AMP activated protein kinase ( AMPK ) . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| Complexes between the LKB 1 tumor suppressor , STRAD alpha / beta and MO 25 alpha / beta are upstream kinases in the AMP activated protein kinase cascade . ^^^ We recently showed that LKB 1 exists as a complex with two accessory subunits , STRAD alpha / beta and MO 25 alpha / beta . ^^^ First , two AMPKK activities purified from rat liver contain LKB 1 , STRAD alpha and MO 25 alpha , and can be immunoprecipitated using anti LKB 1 antibodies . ^^^ Second , both endogenous and recombinant complexes of LKB 1 , STRAD alpha / beta and MO 25 alpha / beta activate AMPK via phosphorylation of Thr 172 . ^^^ Third , catalytically active LKB 1 , STRAD alpha or STRAD beta and MO 25 alpha or MO 25 beta are required for full activity . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that the related STRADbeta and MO25beta isoforms are also able to stabilize LKB 1 in an active complex and that it is possible to isolate complexes of LKB 1 bound to STRAD and MO 25 isoforms , in which the subunits are present in equimolar amounts . ^^^ Our results indicate that MO 25 may function as a scaffolding component of the LKB 1 STRAD complex and plays a crucial role in regulating LKB 1 activity and cellular localization . . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| Par 4 , PAPK , and Mo 25 results in the translocation of LKB 1 from the nucleus to the cytoplasm and to tight junctions and show that the LKB 1 complex may activate MARKs , which are known to introduce 14 3 3 binding sites into several substrates . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| Mouse protein 25 alpha ( MO 25 alpha ) is a 40 kDa protein that , together with the STE 20 related adaptor alpha ( STRAD alpha ) pseudo kinase , forms a regulatory complex capable of stimulating the activity of the LKB 1 tumor suppressor protein kinase . ^^^ MO 25 alpha binds directly to a conserved Trp Glu Phe sequence at the STRAD alpha C terminus , markedly enhancing binding of STRAD alpha to LKB 1 and increasing LKB 1 catalytic activity . ^^^ The data represent a first step toward structural analysis of the LKB 1 STRAD MO 25 complex , and suggests that MO 25 alpha is a scaffold protein to which other regions of STRAD LKB 1 , cellular LKB 1 substrates or regulatory components could bind . . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| Recently , mammalian LKB 1 was found to be active only in a complex with two other proteins STRAD and MO 25 and to induce complete polarization of intestinal epithelial cells in a cell autonomous fashion . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| Endurance training increases LKB 1 and MO 25 protein but not AMP activated protein kinase kinase activity in skeletal muscle . ^^^ LKB 1 complexed with MO 25 and STRAD has been identified as an AMP activated protein kinase kinase ( AMPKK ) . ^^^ We examined trained RQ for altered levels of MO 25 protein and LKB 1 , STRAD , and MO 25 mRNA . ^^^ MO 25 but not LKB 1 or STRAD mRNA increased with training ( P < 0 . 05 ) . ^^^ Trained values ( % controls ) were MO 25 ( 164 + / 22 ) , LKB 1 ( 120 + / 16 ) , and STRAD ( 112 + / 17 ) . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| Analysis of the LKB 1 STRAD MO 25 complex . ^^^ In vivo , LKB 1 forms a complex with STRAD , an inactive pseudokinase , and MO 25 , an armadillo repeat scaffolding like protein . ^^^ Binding of LKB 1 to STRAD MO 25 activates LKB 1 and re localises it from the nucleus to the cytoplasm . ^^^ To learn more about the inherent properties of the LKB 1 STRAD MO 25 complex , we first investigated the activity of 34 point mutants of LKB 1 found in human cancers and their ability to interact with STRAD and MO 25 . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that LKB 1 activates SNRK by phosphorylating the T loop residue ( Thr 173 ) , and that the LKB 1 regulatory subunits STRAD and MO 25 are required for LKB 1 to activate SNRK . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| Gastrocnemius LKB 1 protein , a putative component of the AMPKK complex ( LKB 1 , STRAD , and MO 25 ) , increased approximately twofold in response to training . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| Maximal increases occurred after 4 days for hexokinase 2 , 25 days for MO 25 , and 53 days for citrate synthase , LKB 1 , and PGC 1alpha . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| Human MO 25 is a cofactor of LKB 1 , a conserved protein kinase with roles in cell polarity in nematodes , flies and mammalian cells . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| We identified distinct gene homologues for AMPK alpha , beta and gamma subunits and for LKB 1 , MO 25 and STRAD . ^^^ Genes encoding LKB 1 , MO 25 alpha , MO 25 beta , and STRAD beta were expressed in all examined tissues , whereas STRAD alpha was expressed exclusively in brain , hypothalamus , heart and skeletal muscle . ^^^ |
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| Interacting proteins: Q9Y376 and Q15831 |
Pubmed |
SVM Score :0.0 |
| We also provide evidence that the UBA domains do not function as LKB 1 STRAD ( STE 20 related adaptor ) MO 25 ( mouse protein 25 ) docking / interacting sites and that mutations in the UBA domain of SIK suppressed the ability of SIK to localize within punctate regions of the nucleus . ^^^ |
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