| Interacting proteins: Q16665 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q16665 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In the context of full length HIF 1 alpha , mutation of the leucine residues conferred conformational changes to the protein and significantly reduced the transactivation function as well as functional interaction with the transcriptional coactivators CBP and SRC 1 . ^^^ |
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| Interacting proteins: Q16665 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Six of the genes studied ( aryl hydrocarbon receptor nuclear transporter , aryl hydrocarbon receptor nuclear transporter 2 , Clock , endothelial PAS containing protein , hypoxia inducible factor 1alpha and steroid receptor coactivator 1 ) were expressed in the suprachiasmatic nucleus of adult and neonatal mice . ^^^ |
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| Interacting proteins: Q16665 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Redox regulated recruitment of the transcriptional coactivators CREB binding protein and SRC 1 to hypoxia inducible factor 1alpha . ^^^ Here we demonstrate that two members of the SRC 1 / p160 family of transcriptional coactivators harboring histone acetyltransferase activity , SRC 1 and transcription intermediary factor 2 ( TIF 2 ) , are able to interact with HIF 1alpha and enhance its transactivation potential in a hypoxia dependent manner . ^^^ These data indicate that all three proteins , CBP , SRC 1 , and Ref 1 , are important components of the hypoxia signaling pathway and have a common function in regulation of HIF 1alpha function in hypoxic cells . ^^^ Given the absence of cysteine residues in one of the Ref 1 regulated transactivation domains of HIF 1alpha , it is thus possible that Ref 1 functions in hypoxic cells by targeting critical steps in the recruitment of the CBP SRC 1 coactivator complex . . ^^^ |
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| Interacting proteins: Q16665 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Although an increased association of the two known coactivators of HIF 1 , p 300 and SRC 1 , was found , thyroid hormone did not affect the activity of the isolated COOH terminal transactivating domain of HIF 1alpha . ^^^ |
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| Interacting proteins: Q16665 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In our further exploration of the hypoxia dependent regulation of HIF 1alpha function by transcriptional coactivators we observed that coexpression of SRC 1 ( another important coactivator of the hypoxia response ) and HIF 1alpha did not change the individual characteristic nuclear distribution patterns . ^^^ Biochemical assays showed that depletion of CBP from cell extracts abrogated interaction between SRC 1 and HIF 1alpha . ^^^ Thus , in contrast to the current model for the assembly of complexes between nuclear hormone receptors and coactivators , the present data suggest that it is CBP that recruits SRC 1 to HIF 1alpha in hypoxic cells . ^^^ In conclusion , CBP plays an important role as a mediator of HIF 1alpha / Arnt / CBP / SRC 1 complex formation , coordinating the temporally and hierarchically regulated intranuclear traffic of HIF 1alpha and associated cofactors in signal transduction in hypoxic cells . . ^^^ |
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