| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| STAT 6 interacts only with the PAS B domain of NCoA 1 but not with the homologous regions of NCoA 2 and NCoA 3 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In addition , transcriptional intermediary factor 2 was less dependent on an intact AP 1 response element in the pS 2 promoter than SRC 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Here , we investigated the effect of several coactivators ( p300 / CBP , SRC 1 , TIF 2 , and AIB 1 ) on the transactivation function of CRX . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The three coactivators , TIF 2 , SRC 1 and RAC 3 , are up regulated in relapsed prostate cancer . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| AR coactivators SRC 1 and TIF 2 are up regulated in tissue specimens obtained from patients who failed prostate cancer endocrine therapy . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The three related 160 kDa proteins , SRC 1 , TIF 2 and RAC 3 , were initially identified as factors interacting with nuclear receptors . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Coactivators SRC 1 , TIF 2 , RAC 3 , p 300 , CBP , Tip 60 , and gelsolin are highly expressed in endocrine therapy resistant prostate cancer . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In the epididymis , SRC 1 and TIF 2 immunoreactivities were localized in nuclei of epithelial cells . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Cofactors SRC 1 , RAC 3 , p300 / CBP , TIF 2 , and Tip 60 are upregulated in advanced prostate cancer . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| TIF 2 expression was increased by E 2 , TAM and RLX , but SRC 1 expression was not . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The SRC 1 / NCoA 1 , NCoA 2 / GRIP 1 / TIF 2 , and p / CIP / AIB / ACTR proteins have been shown to act as mediators of transcriptional activation . ^^^ In this report , we demonstrate that SRC 1 , NCoA 2 , and p / CIP are capable of independently enhancing TCDD dependent induction of a luciferase reporter gene by the AHR / ARNT dimer . ^^^ We demonstrate by coimmunoprecipitation and by a reporter gene assay that SRC 1 and NCoA 2 but not p / CIP are capable of interacting with ARNT in vivo after transient transfection into mammalian cells , while AHR is capable of interacting with all three coactivators . ^^^ Furthermore , SRC 1 , NCoA 2 , and p / CIP all associate with the CYP1A1 enhancer region in a TCDD dependent fashion , as demonstrated by chromatin immunoprecipitation assays . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In particular , this article highlights the roles of SRC 1 ( NCoA 1 ) , SRC 2 ( GRIP 1 , TIF 2 , or NCoA 2 ) and SRC 3 ( p / CIP , RAC 3 , ACTR , AIB 1 , or TRAM 1 ) in development , organ function , endocrine regulation , and nuclear receptor function , which are defined by characterization of the genetically manipulated animal models . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| METHODS : Expression vectors for the p 160 CoA genes NCOA 1 , NCOA 2 , or NCOA 3 were transiently transfected into MCF 7 cells . ^^^ RESULTS : Overexpression of NCOA 1 , NCOA 2 , and NCOA 3 enhanced E 2 mediated gene expression by 3 . 17 + / 0 . 51 , 2 . 33 + / 0 . 8 , and 3 . 65 + / 0 . 65 fold , respectively , and enhanced cell survival by suppressing tumor necrosis factor alpha ( TNF alpha ) induced cell death from 80 . 23 % + / 2 . 66 % viability to 101 . 5 % + / 8 . 9 % , 86 . 9 % + / 9 . 9 % , and 95 . 7 % + / 8 . 5 % viability , respectively . ^^^ Clonogenic survival and E 2 stimulated colony formation in MCF 7 cells were suppressed by expression of DI decoy NCOA 1 and DI decoy NCOA 3 to 34 . 4 % + / 7 . 4 % and 54 % + / 5 . 4 % of vector control , but not DI decoy NCOA 2 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The activity of the AF 2 transcriptional activation function of nuclear receptors ( NR ) is mediated by the partially homologous transcriptional coactivators , glucocorticoid receptor interacting protein 1 ( GRIP 1 ) / transcriptional intermediary factor 2 ( TIF 2 ) and steroid receptor coactivator 1 ( SRC 1 ) . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Steroid receptor coactivator 1 ( SRC 1 ) , amplified in breast cancer 1 ( AIB 1 ) , transcriptional intermediary factor 1 ( TIF 1 ) , transcriptional intermediary factor 2 ( TIF 2 ) , and receptor interacting protein 140 ( RIP 140 ) interacted with HEG 0 and L536P HEG 0 in the presence of estradiol , but generally not in the presence of anti estrogens . ^^^ Steroid receptor coactivator 1 ( SRC 1 ) , amplified in breast cancer 1 ( AIB 1 ) , transcriptional intermediary factor 1 ( TIF 1 ) , transcriptional intermediary factor 2 ( TIF 2 ) , and receptor interacting protein 140 ( RIP 140 ) interacted with HEG 0 and L536P HEG 0 in the presence of estradiol , but generally not in the presence of anti estrogens . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Candidate factors have been identified by the observation that changes in glucocorticoid induction parameters in CV 1 cells could be reproduced by varying the cellular levels of coactivators [ transcriptional intermediary factor 2 ( TIF 2 ) , steroid receptor coactivator 1 ( SRC 1 ) , and amplified in breast cancer 1 ( AIB 1 ) ] , comodulator [ CREB binding protein ( CBP ) ] , or corepressor [ silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) ] without concomitant increases in GR . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| RAR coactivators ACTR , SRC 1 , and transcriptional intermediary factor 2 ( TIF 2 ) stimulated human ( h ) SP B promoter activity in a dose dependent fashion in pulmonary adenocarcinoma H 441 cells . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The vitamin D receptor ( VDR ) is the nuclear receptor for 1 , 25 dihydroxyvitamin D ( 3 ) [ 1alpha , 25 ( OH ) ( 2 ) D ( 3 ) ] that acts as a ligand dependent transcription factor via combined contact with coactivator proteins ( steroid receptor coactivator 1 , transcriptional intermediary factor 2 , and receptor associated coactivator 3 ) and specific DNA binding sites [ vitamin D response elements ( VDREs ) ] . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Using Western blot analysis and immunohistochemistry , we demonstrate the expression of the steroid coactivators steroid receptor cofactor ( SRC 1 ) , amplified in breast cancer protein ( AIB 1 ) , and transcriptional intermediary factor 2 ( TIF 2 ) in 81 , 76 , and 76 % of meningiomas , respectively . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Here we report that a majority of recurrent prostate cancers express high levels of the androgen receptor and two nuclear receptor coactivators , transcriptional intermediary factor 2 and steroid receptor coactivator 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Steroid receptor RNA activator ( SRA ) is a novel coactivator for steroid receptors that acts as an RNA molecule , whereas steroid receptor coactivator ( SRC ) family members , such as steroid receptor coactivator 1 ( SRC 1 ) and transcriptional intermediary factor 2 ( TIF 2 ) exert their biological effects as proteins . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In this study using immunohistochemistry and Western blot analysis , we characterize the expression patterns of three coactivators , steroid receptor coactivator 1 , amplified in breast cancer 1 ( AIB 1 ) , and transcriptional intermediary factor 2 in human endometrium obtained prospectively from normal fertile women throughout the menstrual cycle . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| As a result of the proposed allosteric modification of RXR by liganded VDR , the heterodimerized RXR shows the `` phantom ligand effect ' ' and thus acquires the capability to recruit coactivators steroid receptor coactivator 1 , transcriptional intermediary factor 2 , and amplified in breast cancer 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The p 160 coactivators , steroid receptor coactivator 1 ( SRC 1 ) , transcriptional intermediary factor 2 ( TIF 2 ) and receptor associated coactivator 3 ( RAC 3 ) , as well as the coactivator / integrator CBP , mediate estrogen receptor alpha ( ERalpha ) dependent gene expression . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Mammalian two hybrid assays with VP 16 fused VDR and GAL 4 DNA binding domain fused steroid receptor coactivator 1 ( SRC 1 ) , transcriptional intermediary factor 2 ( Tif 2 ) , or DRIP 205 showed the 14 epi analogs to be more potent inducers of VDR coactivator interactions than 1 , 25 ( OH ) 2D3 ( up to 16 and 20 fold stronger induction of VDR SRC 1 interaction for TX 522 and TX 527 at 10 ( 10 ) M ) . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The mouse glucocorticoid receptor interacting protein ( GRIP 1 ) is a member of the ERAP 160 family of nuclear receptor ( NR ) coactivators ( including SRC 1 and TIF 2 ) which function as bridging proteins between ligand activated NRs bound to cognate hormone response elements ( HREs ) and the transcription initiation apparatus ( TIA ) . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| GRIP 1 is the probable ortholog of the subsequently identified human protein transcription intermediary factor 2 ( TIF 2 ) and is also partially homologous to steroid receptor coactivator 1 ( SRC 1 ) . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Subsequently , many more putative co activators have been reported , including the SRC 1 related proteins , TIF 2 and GRIP 1 , and other putative and unrelated co activators such as ARA 70 , Trip 1 , RIP 140 , and TIF 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Scanning surface mutagenesis on the human thyroid hormone receptor was performed to define the site that binds the coactivators , glucocorticoid receptor interacting protein 1 ( GRIP 1 ) and steroid receptor coactivator 1 ( SRC 1 ) . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| SRC 1 and GRIP 1 coactivate transcription with hepatocyte nuclear factor 4 . ^^^ In this study , we show that SRC 1 and GRIP 1 , which act as coactivators for various nuclear receptors , associate with HNF 4 in vivo and enhance its transactivation potential . ^^^ The overexpression of SRC 1 or GRIP 1 enhances expression from a HNF 1 gene promoter reporter in HepG 2 hepatoma cells , and this requires an intact HNF 4 binding site in the HNF 1 gene promoter . ^^^ Thus , HNF 4 is involved in the regulation of glucose homeostasis at several levels and along with the SRC 1 , GRIP 1 , and p 300 may play an important role in the pathophysiology of non insulin dependent diabetes mellitus . . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| As anticipated from its location , the L454S mutant interacts weakly with CoAs , such as SRC 1 and glucocorticoid receptor interacting protein 1 ( GRIP 1 ) in gel mobility shift assays and in mammalian two hybrid assays , even in the presence of the maximal dose of T 3 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Transcriptional coactivators of the p 160 family ( SRC 1 , GRIP 1 , and p / CIP ) associate with DNA bound nuclear receptors ( NRs ) and help the NRs to recruit an active transcription initiation complex to the promoters of target genes . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Yeast two hybrid studies and in vitro protein interaction assays demonstrated that VDR ( Y236A ) was selectively impaired in interaction with AF 2 interacting coactivator proteins such as SRC 1 and GRIP 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Here we report that the C terminal region of p 160 coactivators glucocorticoid receptor interacting protein 1 ( GRIP 1 ) , steroid receptor coactivator 1 ( SRC 1a ) , and SRC 1e binds the N terminal AF 1 activation function of the androgen receptor ( AR ) , and p 160 coactivators can thereby enhance transcriptional activation by AR . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that members of the p 160 family of nuclear receptor coactivators , ACTR ( activator of thyroid and retinoic acid receptors ) , GRIP 1 ( glucocorticoid receptor interacting protein 1 ) , and SRC 1 ( steroid receptor coactivator 1 ) , potentiate the transcriptional activity by hERR 1 and hERR 2 in mammalian cells , and that both orphan receptors bind the coactivators in a ligand independent manner . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we show that GRIP 1 and SRC 1 potentiate the activity of COUP TFI and that COUP TFI associates with these coactivators in vivo using the same region required for transcription activation . ^^^ Finally , overexpression of GRIP 1 or SRC 1 does not convert COUP TFI from a transcriptional repressor into a transcriptional activator in HeLa cells . . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| To this effect , we observed that hPRA , unlike hPRB , was unable to efficiently recruit the transcriptional coactivators GRIP 1 and SRC 1 upon agonist binding . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| We also observed that hTRbeta 1 can bind directly to yeast or human GCN 5 as well as hADA 2 , and that the hGCN 5 ( 387 837 ) sequence could bind directly to either GRIP 1 or SRC 1 coactivator . ^^^ Importantly , the T 3 dependent binding of hTRbeta 1 to hGCN 5 ( 387 837 ) could be markedly increased by the presence of GRIP 1 or SRC 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Collectively , these mutations also suppressed association of VDR with the coactivators GRIP 1 and steroid receptor coactivator 1 in vitro but had little or no effect on ligand binding , heterodimerization with the retinoid 10 receptor , or association with a VDR specific DNA recognition element . ^^^ Co transfection with GRIP 1 or steroid receptor coactivator 1 amplified both the positive and negative responses to wild type VDR but had little or no effect on the functionally impaired mutants described above . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Furthermore , using cotransfection and antisense technology we have found that , unlike SRC 1 and GRIP 1 , which are not involved in the GR complex that suppresses keratin genes , histone acetyltransferase and CBP are . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Constitutive uterine mRNA expression of switch protein for antagonist ( SPA ) , SRC 1 , GRIP 1 , RAC 3 , RIP 140 , and p 300 mRNAs was observed in control uteri , and treatment with ER ligands did not alter coactivator mRNA levels . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Members of the p 160 coactivator family ( steroid receptor coactivator 1 ( SRC 1 ) , glucocorticoid receptor interacting protein 1 ( GRIP 1 ) , and activator of thyroid and retinoic acid receptors ( ACTR ) ) mediate transcriptional activation by nuclear receptors . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| To investigate the molecular mechanisms underlying the ER subtype selective actions of these compounds , we have determined the conformational changes induced in ERalpha and ERbeta by these ligands using protease digestion sensitivity , and we have tested the ability of these ligands to promote the recruitment of representatives of the three SRC / p160 coactivator protein family members ( SRC 1 , GRIP 1 , ACTR , respectively ) to ERalpha and ERbeta using yeast two hybrid and glutathione S transferase ( GST ) pull down assays . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| To determine whether this enhanced activity is mediated through modulation of the dimerization process or through interaction with coactivators , we performed quantitative protein protein interaction assays with in vitro translated vitamin D receptor ( ivtVDR ) and fusion proteins containing glutathione S transferase ( GST ) and either the ligand binding domain of retinoid 10 receptor ( RXRalpha ) , or the nuclear receptor interacting domain of the steroid receptor coactivator 1 ( SRC 1 ) , or the glucocorticoid receptor interacting protein 1 ( GRIP 1 ) . ^^^ We found that heterodimerization of the ligand binding domains of RXRalpha and VDR was primarily deltanoid dependent as was the interaction of VDR with the SRC 1 or with GRIP 1 . ^^^ However , the ED 50 for induction of VDR interaction with SRC 1 was similar for both 1 , 25D3 and the 20 epi analog ( ED 50 = 0 . 7 1 . 0 nM ) as was the ED 50 for ligand mediated interaction of VDR with GRIP 1 ( ED 50 = 0 . 1 0 . 3 nM ) . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The coactivator CARM 1 methylates histone H 3 at Arg 17 and Arg 26 in vitro and cooperates synergistically with p 160 type coactivators ( e . g . , GRIP 1 , SRC 1 , ACTR ) and coactivators with histone acetyltransferase activity ( e . g . , p 300 , CBP ) to enhance gene activation by steroid and nuclear hormone receptors ( NR ) in transient transfection assays . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In addition , NH 3 prevents 10 . laevis thyroid hormone receptors from binding to the p 160 family of co activators GRIP 1 and SRC 1 in a two hybrid assay . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Reproductive tracts were collected , weighed , and examined for changes in histomorphology and expression of ER and nuclear receptor co regulators ( SRC 1 , p 300 , CARM 1 , GRIP 1 , SPA , REA and Uba 3 ) . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In contrast , at the osteocalcin gene GRE , where GR represses transcription by binding to a DNA site overlapping the TATA box , both GRIP 1 and SRC 1 corepressed , and the GRIP 1 specific repression domain was dispensable . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Furthermore , IA R bound ERalpha L384M and wild type ERbeta displayed enhanced interactions with the nuclear receptor interaction domains of the coactivators SRC 1 and GRIP 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Protein fragments harboring the LXXLL motifs of the coactivators GRIP 1 and SRC 1 or TRAP 220 interacted predominantly with the TR . retinoid 10 receptor heterodimeric pair in a ligand dependent fashion . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| One important coactivator complex includes a p 160 coactivator ( e . g . , GRIP 1 , SRC 1 , or ACTR ) that binds directly to activated NR , the histone acetyltransferase p 300 or CBP , and the arginine specific histone methyltransferase CARM 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The p 160 coactivators , steroid receptor coactivator 1 , glucocorticoid receptor interacting protein 1 ( GRIP 1 ) and the activator of thyroid and retinoic acid receptor , have two activation domains , AD 1 and AD 2 , which transmit the activation signal from the DNA bound nuclear receptor to the chromatin and / or transcription machinery . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The p 160 co activators , SRC 1 ( steroid receptor co activator 1 ) , GRIP 1 ( glucocorticoid receptor interacting protein 1 ) and ACTR ( activator for thyroid hormone and retinoid receptors ) , have two ADs ( activation domains ) , AD 1 and AD 2 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Glutathione S transferase pull down experiments showed that SRC 1 physically interacted with the activation domain of STAT 3 and that chromatin immunoprecipitation experiments detected the occupancy of SRC 1 , but not GRIP 1 or AIB 1 , on the promoter of STAT 3 target genes . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| CHO K 1 cells transfected with ERalpha or ERbeta show ERE sequence dependent differences in the functional interaction of ERalpha and ERbeta with coactivators steroid receptor coactivator 1 ( SRC 1 ) , SRC 2 ( glucocorticoid receptor interacting protein 1 ( GRIP 1 ) ) , SRC 3 amplified in breast cancer 1 ( AIB 1 ) and ACTR , cyclic AMP binding protein ( CBP ) , and steroid receptor RNA activator ( SRA ) , corepressors nuclear receptor co repressor ( NCoR ) and silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) , and secondary coactivators coactivator associated arginine methyltransferase 1 ( CARM 1 ) and protein arginine methyltransferase 1 ( PRMT 1 ) . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Although RA receptor gamma and glucocorticoid receptor bind to the same response element repressing transcription of keratins K6 / K16 , RA receptor interacts with the components of the EGF enhanceosome ( co activators : glucocorticoid receptor interactive protein 1 ( GRIP 1 ) / steroid receptors coactivator 1 ( SRC 1 ) ) without breaking it . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The functional synergy of the p 160 coactivators [ steroid receptor coactivator 1 , glucocorticoid receptor interacting protein ( GRIP 1 ) , or the activator for thyroid hormone and retinoid receptors ] , the histone acetyltransferases cAMP response element binding protein binding protein ( CBP ) and p 300 and the histone methyltransferase coactivator associated arginine methyltransferase ( CARM 1 ) depends on the methyltransferase activity of CARM 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| One important coactivator complex includes a p 160 coactivator ( GRIP 1 , SRC 1 , or ACTR ) and its downstream coactivators ( e . g . , p 300 , CARM 1 , CoCoA , and Fli 1 ) , which contribute to transcriptional activation by protein acetylation , protein methylation , and protein protein interactions . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| We report here that overexpression of steroid receptor coactivator 1 ( SRC 1 ) and GR interacting protein 1 ( GRIP 1 ) enhanced repression by liganded GR , and by a GR mutant defective in repression . ^^^ Surprisingly , SRC 1 and GRIP 1 also enhanced TGFbeta induced activation from the TGFbeta responsive sequence of the PAI 1 gene by a GR independent mechanism . ^^^ Coimmunoprecipitation and mammalian one hybrid experiments demonstrated that SRC 1 and GRIP 1 interact physically with endogenous Smad 3 and functionally with the C terminal domain of Smad 3 to directly enhance transcription . ^^^ Thus , the GR coactivators , SRC 1 and GRIP 1 , act as both corepressors of the glucocorticoid repression of PAI 1 gene transcription , and coactivators of TGFbeta induced activation of the PAI 1 promoter . . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation assays further demonstrate that MUC 1 ( 1 ) associates with ERalpha complexes on estrogen responsive promoters , ( 2 ) enhances ERalpha promoter occupancy , and ( 3 ) increases recruitment of the p 160 coactivators SRC 1 and GRIP 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Using mammalian two hybrid assays , we show that zearalenone displaces the nuclear receptor corepressor protein N CoR from hPXR , while it recruits coactivator proteins steroid receptor coactivator 1 , Glucocorticoid Receptor Interacting Protein 1 and PPAR Binding protein ( GRIP 1 ) and PBP to hPXR . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Here , we showed that AIB 1 was a sumoylated protein and the sumoylation attenuated the transactivation activity of AIB 1 , which is in contrast to the sumoylation of its paralogs , GRIP 1 and SRC 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| TIF 2 exhibits partial sequence homology with the recently isolated steroid receptor coactivator SRC 1 , indicating the existence of a novel gene family of nuclear receptor transcriptional mediators . . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| RAC 3 , a steroid / nuclear receptor associated coactivator that is related to SRC 1 and TIF 2 . ^^^ Sequence analysis reveals that RAC 3 is related to steroid receptor coactivator 1 ( SRC 1 ) and transcriptional intermediate factor 2 ( TIF 2 ) , two of the most potent coactivators for steroid / nuclear receptors . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Expression of RNA encoding TIF 2 , a member of the SRC 1 family , was increased in the SRC 1 null mutant , perhaps compensating partially for the loss of SRC 1 function in target tissues . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| By sequence comparison with the related coactivator SRC 1 , we identified three short conserved motifs ( NR boxes ) in both proteins which are the putative binding sites of TIF 2 to nuclear hormone receptors . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In addition , we demonstrate that liganded PR is present in stable complexes containing SRC 1 and transcription intermediary factor 2 ( TIF 2 ) in vivo . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The coactivators used in this study contained CBP , p 300 , RIP 140 , SRC 1 , TIF 1 , and TIF 2 . ^^^ By two hybrid assay in yeast , the interactions of estrogen receptor with RIP 140 , SRC 1 , TIF 1 , and TIF 2 were detected and they were completely dependent on the presence of estrogen . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| A family of cotranscriptional activators ( SRC 1 , TIF 2 , and AIB 1 ) interacts with and activates the transactivation function of nuclear receptors in a ligand dependent way . ^^^ Unlike other vitamin D analogs tested , OCT ( 22 oxa 1alpha , 25 dihydroxyvitamin D 3 ) induced interaction of VDR with TIF 2 but not with SRC 1 or AIB 1 . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The MAbs were shown to be specific to AIB 1 and did not cross react with two similar coactivators SRC 1 and TIF 2 as shown in Western blot analysis and enzyme linked immunoadsorbent assay ( ELISA ) . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that two members of the SRC 1 / p160 family of transcriptional coactivators harboring histone acetyltransferase activity , SRC 1 and transcription intermediary factor 2 ( TIF 2 ) , are able to interact with HIF 1alpha and enhance its transactivation potential in a hypoxia dependent manner . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| To obtain some clue to these roles , we screened the expression levels of ER alpha , ER beta , coactivators ( SRC 1 , TIF 2 , AIB 1 , CBP , and P / CAF ) and corepressors ( N CoR and SMRT ) in 6 normal mammary glands , 6 intraductal carcinomas , 22 invasive ductal carcinomas , and 7 breast cancer cell lines using a multiplex reverse transcription PCR . ^^^ |
|
| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| However , ZK 159222 was not able to promote a ligand dependent interaction of the VDR with the coactivator proteins SRC 1 , TIF 2 , and RAC 3 , neither in solution nor in a complex with RXR on DNA . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| To examine this hypothesis , we cloned rat complementary DNA fragments corresponding to coactivators ( SRC 1 , TIF 2 and TRAM 1 ) and corepressors ( N CoR and SMRT ) , and studied the ontogenic changes in their corresponding messenger RNAs in rat cerebellum of normal and hypothyroid rats during postnatal development , using a RNase protection assay . ^^^ We found an increased expression of SRC 1 and TIF 2 , as well as of N CoR , during rat cerebellar development but no change in the expression of SMRT and TRAM 1 genes . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Confirmed and putative HAT proteins have been identified from various organisms from yeast to humans , and they include Gcn 5 related N acetyltransferase ( GNAT ) superfamily members Gcn 5 , PCAF , Elp 3 , Hpa 2 , and Hat 1 : MYST proteins Sas 2 , Sas 3 , Esa 1 , MOF , Tip 60 , MOZ , MORF , and HBO 1 ; global coactivators p 300 and CREB binding protein ; nuclear receptor coactivators SRC 1 , ACTR , and TIF 2 ; TATA binding protein associated factor TAF ( 2 ) 250 and its homologs ; and subunits of RNA polymerase 3 general factor TFIIIC . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Moreover , all three members of the p 160 family of nuclear receptor coactivators , SRC 1 , TIF 2 , and RAC 3 , are able to potentiate transcription from a TEF response element in transient transfection experiments , and this activation requires the presence of the bHLH PAS domain . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The natural ligand 15 deoxy Delta 12 , 14 prostaglandin J ( 2 ) induced PPARgamma interactions with all coactivators tested ( SRC 1 , TIF 2 , AIB 1 , p 300 , TRAP220 / DRIP205 ) in yeast and mammalian two hybrid assays , as well as in a glutathione S transferase pull down assay . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Ten of the 12 cofactors tested were expressed in all cells analyzed ( AIB 1 , ARA 54 , ARA 70 , CBP , cyclin D 1 , Her2 / neu / erbB2 , BAG 1 / M / L , SRC 1 , SMRT , and TIF 2 ) . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| METHODS : Using reverse transcriptase polymerase chain reaction , we examined the expression levels of AR coactivators ( ARA 55 , SRC 1 , ARA 54 , TIF 2 , RAC 3 ) in four prostate cancer cell lines ( DU 145 , PC 3 , LNCaP , and LN TR 2 ) , nine benign prostatic tissue samples , and 21 prostate cancer tissue specimens . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Co transfections of coactivators such as SRC 1 , TIF 2 , AIB 1 , and TRAP 220 in 293T cells and use of the luciferase assay revealed that TRAP 220 failed to enhance the transcription mediated by ERbeta in the presence of ferutinine . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| We constructed two hybrid systems that co express the Gal4p DNA binding domain / ligand binding domain of human estrogen receptor ( hER ) alpha or beta and the Gal4p transactivation domain / nuclear receptor binding domain of co activator SRC 1 , TIF 2 , or AIB 1 in Saccharomyces cerevisiae with a chromosome integrated lacZ reporter gene under the control of Gal4p binding sites . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Thyroid function in mice with compound heterozygous and homozygous disruptions of SRC 1 and TIF 2 coactivators : evidence for haploinsufficiency . ^^^ We have investigated their possible redundancy as thyroid hormone ( TH ) coactivators by measuring thyroid function in compound SRC 1 and TIF 2 knock out ( KO ) mice . ^^^ Whereas SRC 1 KO ( SRC 1 ( / ) ) mice are resistant to TH and SRC 1 ( + / ) are not , we now demonstrate that TIF 2 KO ( TIF 2 ( / ) ) mice have normal thyroid function . ^^^ Yet double heterozygous , SRC 1 ( + / ) / TIF 2 ( + / ) mice manifested resistance to TH of a similar degree as that in mice completely deficient in SRC 1 . ^^^ KO of both SRC 1 and TIF 2 resulted in marked increases of serum TH and thyrotropin concentrations . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Furthermore , not only AR but also the glucocorticoid receptor YFP , ER alpha GFP , and YFP tagged SRC 1 , TIF 2 , and CBP were found to be accumulated in identical spots in the presence of ligand . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Steroid receptor coactivator 1 and transcription intermediary factor 2 ( TIF 2 ) belong to the p 160 coactivator family that mediates transcriptional activation by several nuclear receptors , including SF 1 . ^^^ Thus , in contrast to the regulation of p / CIP and steroid receptor coactivator 1 , we suggest that activation of PKA leads to selective down regulation of TIF 2 and subsequently repression of TIF 2 coactivator function . . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Human TIF 2 ( hTIF 2 ) is a member of the p 160 family of nuclear receptor coactivators , which includes SRC 1 and p / CIP . ^^^ We conclude that TIF 2 plays a critical role in mouse reproductive functions , whereas previous reports have not revealed serious fertility impairment in SRC 1 ( / ) or p / CIP ( / ) mutants . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Coactivators such as TIF 2 and SRC 1 modulate the positioning of the dose response curve for agonist bound glucocorticoid receptors ( GRs ) and the partial agonist activity of antiglucocorticoid complexes . ^^^ We now report that constructs of TIF 2 and SRC 1 lacking the two activation domains ( AD 1 and AD 2 ) have significantly less ability to increase transactivation but retain most of the activity for modulating the dose response curve and partial agonist activity . ^^^ The absence of synergism by PCAF or DRIP 150 with SRC 1 or TIF 2 , respectively , further suggests that these other factors are not involved . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| SRC 1 and TIF 2 control energy balance between white and brown adipose tissues . ^^^ TIF 2 / mice are protected against obesity and display enhanced adaptive thermogenesis , whereas SRC 1 / mice are prone to obesity due to reduced energy expenditure . ^^^ In white adipose tissue , lack of TIF 2 decreases PPARgamma activity and reduces fat accumulation , whereas in brown adipose tissue it facilitates the interaction between SRC 1 and PGC 1alpha , which induces PGC 1alpha ' s thermogenic activity . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In a second transgenic mouse line , an enhancer region , which binds to thyroid transcription factor 1 , retinoic acid receptor , signal transducers and activators of transcription 3 , and nuclear receptor coactivators ( SRC 1 , ACTR , TIF 2 , and CBP / p300 ) , was deleted from the hSP B 1 . 5 kb lacZ gene . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The aim of this study was to examine the expression of four steroid receptor co activators ( steroid receptor co activator 1 ( SRC 1 ) , transcription intermediary factor 2 ( TIF 2 ) , protein 300 kDa / CREB binding protein ( p300 / CBP ) , amplified in breast cancer 1 ( AIB 1 ) ) , and of the co repressor nuclear receptor co repressor ( NCoR ) , in malignant breast tissues and in matching normal breast biopsies of the same individuals . ^^^ The tumoral ER alpha protein expression was significantly correlated with that of PgR ( r = 0 . 61 , p = 0 . 001 ) and NCoR ( r = 0 . 4 , p = 0 . 043 ) , whereas ER beta expression was associated with SRC 1 ( r = 0 . 68 , p < or = . 001 ) , TIF 2 ( r = 0 . 64 , p = 0 . 001 ) and NCoR ( r = 0 . 48 , p = 0 . 014 ) protein levels in malignant specimens . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Various kinds of cofactors , such as steroid receptor coactivator 1 ( SRC 1 ) , transcription intermediary factor 2 ( TIF 2 ) , and amplified in breast cancer 1 ( AIB 1 ) , have also been reported . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| We have determined the effect of TH withdrawal and treatment on the expression of different isoforms of TR as well as expression of the NCoAs SRC 1 , TIF 2 and SRC 3 using quantitative real time polymerase chain reaction . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| At the start of the initiation step of transcription , nuclear receptors interact with coactivators ( TIF 2 , SRC 1 , ACTR , CBP / p300 , etc . ) in an agonist dependent manner . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| EXPERIMENTAL DESIGN : The expression of 16 AR coactivators and corepressors ( SRC 1 , beta catenin , TIF 2 , PIAS 1 , PIASx , ARIP 4 , BRCA 1 , AIB 1 , AIB 3 , CBP , STAT 1 , NCoR 1 , AES , cyclin D 1 , p 300 , and ARA 24 ) was measured in prostate cancer cell lines , xenografts , and clinical prostate tumor specimens by using real time quantitative reverse transcription PCR . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| At the molecular level , this pathway requires stabilization of the homodimer DNA complexes through ligand dependent interaction with the coactivator SRC 1 or TIF 2 . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Elevated levels of MR , the co activators TIF 2 and SRC 1 , and the co repressor SMRT do modulate the dose response curve and partial agonist activity of MR complexes . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| To quantify their roles in the function of androgen receptor ( AR ) transcriptional activity in vivo , we generated a unique transgenic AR reporter mouse and analyzed the cell specific contributions of SRC 1 and TIF 2 to the activity of AR in mouse testis . ^^^ Although SRC 1 concentrates in Sertoli cell nuclei in the absence of TIF 2 , nuclear SRC 1 is not able to rescue AR activity in the TIF 2 mutant background . ^^^ Interestingly , SRC 1 appears to negatively influence AR activity , thereby counterbalancing the TIF 2 stimulated AR activity . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The related p 160 protein TIF 2 does not interact with TDG and has the altered sequence , F 10 10 10 Y , at the equivalent positions relative to SRC 1 . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| A combination of the full length human estrogen receptor alpha with the nuclear receptor binding domain of co activator steroid receptor co activator 1 ( SRC 1 ) or transcriptional intermediate factor 2 ( TIF 2 ) was most effective for estrogen dependent induction of the chromosome integrated UAS ( GAL ) CYC 1 ( p ) lacZ reporter construct among the two hybrid systems so far tested . . ^^^ |
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| Interacting proteins: Q15596 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation analyses showed that DHT increased the recruitment of AR and coactivators , such as SRC 1 and TIF 2 , to the promoter of the PSA gene , and that recruitment was greatly diminished in the presence of 5 micromol / L selenium . ^^^ |
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