| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The two hybrid system also revealed that the interaction between VDR and SRC 1 was reduced by TEI 9647 in Saos 2 cells . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The SRC 1 binds the vitamin D receptor ( VDR ) in the presence of ligand in an activation function 2 ( AF 2 ) dependent manner . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.53537033 |
| We found that the human steroid receptor coactivator 1 ( SRC 1 ) interacts with the VDR in a ligand dependent manner , as demonstrated by beta galactosidase production . 0.53537033^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.54959569 |
| The helix 3 and helix 12 mutations strikingly reduced the ability of VDR to interact with the coactivators steroid receptor coactivator 1 , ACTR , and the CREB binding protein . 0.54959569^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.66093162 |
| The vitamin D receptor ( VDR ) is the nuclear receptor for 1 , 25 dihydroxyvitamin D ( 3 ) [ 1alpha , 25 ( OH ) ( 2 ) D ( 3 ) ] that acts as a ligand dependent transcription factor via combined contact with coactivator proteins ( steroid receptor coactivator 1 , transcriptional intermediary factor 2 , and receptor associated coactivator 3 ) and specific DNA binding sites [ vitamin D response elements ( VDREs ) ] . 0.66093162^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.51910339 |
| However , the ED 50 for induction of VDR interaction with SRC 1 was similar for both 1 , 25D3 and the 20 epi analog ( ED 50 = 0 . 7 1 . 0 nM ) as was the ED 50 for ligand mediated interaction of VDR with GRIP 1 ( ED 50 = 0 . 1 0 . 3 nM ) . 0.51910339^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| All AF 2 mutations that abolished VDR mediated transactivation also eliminated interactions between VDR and several putative coactivator proteins including suppressor of gal 1 ( SUG 1 ) , steroid hormone receptor coactivator 1 ( SRC 1 ) , or receptor interacting protein ( RIP 140 ) , suggesting that coactivator interaction is important for AF 2 mediated transcription . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Unlike other vitamin D analogs tested , OCT ( 22 oxa 1alpha , 25 dihydroxyvitamin D 3 ) induced interaction of VDR with TIF 2 but not with SRC 1 or AIB 1 . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Smad 3 , one of the SMAD proteins downstream in the TGF beta signaling pathway , was found in mammalian cells to act as a coactivator specific for ligand induced transactivation of VDR by forming a complex with a member of the steroid receptor coactivator 1 protein family in the nucleus . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Yeast two hybrid studies and in vitro protein interaction assays demonstrated that VDR ( Y236A ) was selectively impaired in interaction with AF 2 interacting coactivator proteins such as SRC 1 and GRIP 1 . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Indeed , expression of the coactivators , steroid hormone receptor coactivator 1 ( SRC 1 ) and CREB binding protein ( CBP ) , significantly enhances the stimulatory effect of vitamin D mediated by the wild type VDR but not by the AF 2 mutant receptor . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In this report , we analyzed the effect of nuclear receptor coactivators ( SRC 1 and TRAM 1 ) on VDR homodimer and VDR / RXR heterodimer formation by electrophoretic mobility shift assay . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| However , ZK 159222 was not able to promote a ligand dependent interaction of the VDR with the coactivator proteins SRC 1 , TIF 2 , and RAC 3 , neither in solution nor in a complex with RXR on DNA . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Collectively , these mutations also suppressed association of VDR with the coactivators GRIP 1 and steroid receptor coactivator 1 in vitro but had little or no effect on ligand binding , heterodimerization with the retinoid 10 receptor , or association with a VDR specific DNA recognition element . ^^^ Co transfection with GRIP 1 or steroid receptor coactivator 1 amplified both the positive and negative responses to wild type VDR but had little or no effect on the functionally impaired mutants described above . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| No significant differences in the 1alpha , 25 ( OH ) ( 2 ) D ( 3 ) modulated interactions of the DR 3 type VDRE complexed VDR RXR heterodimer with the co activator SRC 1 ( steroid receptor co activator 1 ) or the co repressor NCoR ( nuclear receptor co repressor ) were found . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| This study demonstrates that the interaction of the VDR with NCoR results in a preferential stabilization of the VDR in a non agonistic conformation ( silent state ) , whereas within a complex with SRC 1 VDR is in its agonistic conformation ( activated state ) . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Subsequent studies demonstrated that the mutant VDR had a marked impairment in binding steroid receptor coactivator 1 ( SRC 1 ) and DRIP 205 , a subunit of the vitamin D receptor interacting protein ( DRIP ) coactivator complex . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| A cyclic pattern of promoter occupancy by VDR , SRC 1 , and NCoA62 / SKIP was observed , with NCoA62 / SKIP entering these promoter complexes after SRC 1 . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| As a result of the proposed allosteric modification of RXR by liganded VDR , the heterodimerized RXR shows the `` phantom ligand effect ' ' and thus acquires the capability to recruit coactivators steroid receptor coactivator 1 , transcriptional intermediary factor 2 , and amplified in breast cancer 1 . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| RESULTS : 1 , 25 ( OH ) 2D3 induces rapid association of the VDR and RXR with both the Cyp 24 and the Opn gene promoters in both MC3T3 E 1 osteoblasts and MOBs , interactions that are both rapid and cyclic in nature . 1 , 25 ( OH ) 2D3 treatment also induces rapid recruitment of co regulators such as SRC 1 , 2 , and 3 , CBP , and p 300 to both promoters , recruitment that leads to acetylation of histone 4 on Cyp 24 but not the Opn . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Mammalian two hybrid assays with VP 16 fused VDR and GAL 4 DNA binding domain fused steroid receptor coactivator 1 ( SRC 1 ) , transcriptional intermediary factor 2 ( Tif 2 ) , or DRIP 205 showed the 14 epi analogs to be more potent inducers of VDR coactivator interactions than 1 , 25 ( OH ) 2D3 ( up to 16 and 20 fold stronger induction of VDR SRC 1 interaction for TX 522 and TX 527 at 10 ( 10 ) M ) . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Bufalin did not bind directly to VDR but did modulate the interaction of VDR and cofactors , such as steroid receptor coactivator 1 and nuclear receptor corepressor . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| VDR and C / EBP alpha were recruited concurrently to the composite element along with the coactivators p 300 , steroid receptor coactivator 1 ( SRC 1 ) , and SRC 2 , but not SRC 3 . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Moreover , re ChIP assays confirmed the functionality of the three 1alpha , 25 ( OH ) 2D3 reponsive promoter regions by monitoring simultaneous occupancy of VDR with the co activator proteins CBP , SRC 1 and TRAP 220 . ^^^ |
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| Interacting proteins: P11473 and Q15788 |
Pubmed |
SVM Score :0.0 |
| A final ChIP assay revealed that VDR / RXR heterodimer binding to the TRPV 6 gene was accompanied by both the recruitment of steroid receptor coactivator 1 as well as a broad change in histone 4 acetylation . ^^^ |
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