| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.66268158 |
| Steroid receptor coactivator 1 ( SRC 1 ) has been shown to interact with the human AR and to modulate ligand dependent AR transactivation and is regulated by phosphorylation by MAPK . 0.66268158^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.86197798 |
| Thus the AR interacts with SRC 1 via two different regions and the AF 1 interaction is functionally the more important , although the contribution of the two interactions varies in a promoter dependent fashion . 0.86197798^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| The most striking difference was seen with the androgen receptor , which bound well to GRIP 1 but poorly to SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Full length TRAM 1 ( aa 1 1424 ) and the partial ( aa 459 1424 ) were AR and GR coactivators as was SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Kinetic analysis revealed dissociation constants ( KD ) of 9 cis RA preincubated RXR to SRC 1 was 5 . 92 10 10 ( 8 ) M . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Additional work , including 5 ' RACE , has characterized a full length cDNA that encodes a approximately 160 kD protein as a putative thyroid hormone receptor coactivator ( F SRC 1 ) . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| CBP or F SRC 1 not only enhanced AR mediated transactivation , but also facilitated the androgen dependent interaction between the N and C terminal domains , implying that part of the coactivator dependent transcriptional activation occurs via this mechanism . ^^^ In contrast , SRC 1 , a coactivator for the progesterone receptor , inhibited both AR mediated transactivation and interaction between the N and C termini . ^^^ Recruitment of coregulators may involve AR domains other than the LBD , as F SRC 1 and CBP enhanced , but SRC 1 repressed , the transcriptional activity of ARDelta 641 902 . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Co expression of ARA 54 with other AR coactivators , such as ARA 70 or SRC 1 , showed additive stimulation of AR mediated transactivation , which indicates that these cofactors may function individually as AR coactivators to induce AR target gene expression . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| The AF 1 and AF 2 domains of the androgen receptor interact with distinct regions of SRC 1 . ^^^ Although SRC 1 is capable of interacting with the ligand binding domain by means of LXXLL motifs , this interaction is not essential since an SRC 1 mutant with no functional LXXLL motifs retains its ability to potentiate androgen receptor activity . ^^^ In contrast , mutants lacking the glutamine rich region are inactive , indicating that this region is both necessary and sufficient for recruitment of SRC 1 to the androgen receptor . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| A C619Y mutation in the human androgen receptor causes inactivation and mislocalization of the receptor with concomitant sequestration of SRC 1 ( steroid receptor coactivator 1 ) . ^^^ Interestingly , these aggregates also contain the bulk of the coexpressed steroid receptor coactivator SRC 1 , suggesting , in analogy to AR in spinal bulbar muscular atrophy , that this mutant may alter cellular physiology through sequestration of critical proteins . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| We also found that ARA 70 , ARA 55 , and ARA 54 , but not steroid receptor coactivator 1 ( SRC 1 ) and Rb , could significantly enhance the delta 5 androstenediol mediated AR transactivation . ^^^ Furthermore , in comparing the relative specificity of these coactivators to AR in DU 145 cells , our results suggested that ARA 70 has a relatively higher specificity and that SRC 1 can enhance almost equally well many other steroid receptors . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| METHODS : Using reverse transcriptase polymerase chain reaction , we examined the expression levels of AR coactivators ( ARA 55 , SRC 1 , ARA 54 , TIF 2 , RAC 3 ) in four prostate cancer cell lines ( DU 145 , PC 3 , LNCaP , and LN TR 2 ) , nine benign prostatic tissue samples , and 21 prostate cancer tissue specimens . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Unlike the steroid hormone receptor coactivator 1 ( SRC 1 ) , beta catenin showed a strong interaction with AR but not with other steroid hormone receptors such as estrogen receptor alpha , progesterone receptor beta , and glucocorticoid receptor . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Unlike SRC 1 that can enhance the interaction between AR N terminus and AR C terminus , SV shows a mild suppressive effect on N C interactions , suggesting SV may go through a different mechanism to enhance AR transactivation . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Furthermore , not only AR but also the glucocorticoid receptor YFP , ER alpha GFP , and YFP tagged SRC 1 , TIF 2 , and CBP were found to be accumulated in identical spots in the presence of ligand . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| However , acetylation deficient AR mutants were selectively defective in DHT induced trans activation of androgen responsive reporter genes and coactivation by SRC 1 , Ubc 9 , TIP 60 , and p 300 . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Nonetheless , bicalutamide could not stimulate interactions between the AR N and C termini or recruitment of steroid receptor coactivator proteins ( SRC 1 or 2 ) , although SRC transfection augmented AR activity in the presence of dihydrotestosterone and inhibitory concentrations of bicalutamide . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| A marked decline in the expression of CBP / p300 and SRC 1 in the aged SNB motoneurons suggests down regulation of androgen receptor coactivator mediated gene expression in the SNB system with advancing age . . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Our results revealed nearly constant expression of AR and heterogeneous expression of AR co factors , with increased expression of PIAS 1 and Ran / ARA24 , decreased expression of ELE1 / ARA70 , and no change in TMF1 / ARA160 , ARA 54 , SRC 1 , or TRAP 220 . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| The presence of the steroid receptor coactivator SRC 1 in the telencephalic song control nuclei and in the catecholaminergic cell groups that innervate the song system supports the idea that SRC 1 expression could play an active role in the control of singing behavior by modulating estrogen and androgen receptor action at both locations . . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| AR ( M749L ) transactivation can be further enhanced in the presence of AR coregulators , such as ARA 70 and SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| The dynamic interaction between the androgen receptor ( AR ) and steroid receptor coactivator 1 ( SRC 1 ) was explored in living cells expressing chimeric forms of the receptor and the coactivator containing two spectral variants of jellyfish fluorescent protein . ^^^ Activation and nuclear import of CFP AR by the agonistic ligand 5alpha dihydrotestosterone , but not by the antagonist casodex , transferred YFP SRC 1 from the PML bodies to an interlacing filamentous structure . ^^^ In a single living cell , agonist activated AR caused a time dependent movement of YFP SRC 1 from the PML bodies to this filamentous structure . ^^^ Additionally , coexpression of a constitutively active mutant of AR ( AR deltaligand binding domain ) also displaced YFP SRC 1 from the PML bodies to this intranuclear filamentous structure . ^^^ The fluorescence recovery after photobleaching approach was used to examine changes in the kinetics of movement of YFP SRC 1 during its mobilization from the PML bodies to the intranuclear filamentous structure by the agonist activated AR . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| As low levels of androgen receptor were observed in the cultures , we also measured transcript levels for steroid receptor coactivators SRC 1 , ARA 70 , and AIB 1 . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| METHODS : AR and coactivators ARA 54 , ARA 55 , ARA 70 , and SRC 1 RNA were analyzed by RT PCR in normal and tumoral tissues of the same prostate , in prostate cell lines , and after hormonal treatments of prostate epithelial cells . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| EXPERIMENTAL DESIGN : The expression of 16 AR coactivators and corepressors ( SRC 1 , beta catenin , TIF 2 , PIAS 1 , PIASx , ARIP 4 , BRCA 1 , AIB 1 , AIB 3 , CBP , STAT 1 , NCoR 1 , AES , cyclin D 1 , p 300 , and ARA 24 ) was measured in prostate cancer cell lines , xenografts , and clinical prostate tumor specimens by using real time quantitative reverse transcription PCR . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Here we show by a modified mammalian two hybrid system that both the AR interacting domains of the coactivator SRC 1 and of the corepressor SMRT compete for interaction with the AR N terminus . ^^^ We show that the Qr region of SRC 1 is able to inhibit the interaction of SMRT with AR . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| The CPA liganded AR was coactivated by steroid receptor coactivator 1 ( SRC 1 ) but did not mediate N C terminal interactions or recruit beta catenin , indicating a nonagonist conformation . ^^^ The HF and CPA liganded T877A ARs were coactivated by SRC 1 , but only the HF liganded T877A AR was coactivated by beta catenin . ^^^ L 39 , a novel AR antagonist that transcriptionally activates the T877A AR , but still inhibits LNCaP growth , similarly mediated recruitment of SRC 1 and not beta catenin . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| The results showed that AR coactivators such as ARA70N , gelsolin , ARA 54 , and SRC 1 can enhance AR transactivation but showed differential influences on the N C interaction . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| We hypothesized that daylength regulates the expression of androgen receptor ( AR ) and / or steroid receptor coactivator 1 ( SRC 1 ) in specific forebrain regions . ^^^ The present results indicate that daylength induced fluctuations in SRC 1 and AR expression may contribute to seasonally changing effects of testosterone . . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| In similar assays , there was reduced binding of the p 160 coactivators TIF2 / SRC2 and SRC 1 to the mutant AR ligand binding domains ( LBD ) . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| AR coactivators SRC 1 and TIF 2 are up regulated in tissue specimens obtained from patients who failed prostate cancer endocrine therapy . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Anisotropy measurements revealed that the affinity of this PGC 1alpha fragment for human ERalpha and beta was fairly strong in the presence of estradiol ( approximately 5 nM ) , and that unlike a similar fragment of SRC 1 ( 570 780 ) , PGC 191 408 exhibited ligand independent interactions with ER , particularly with ERbeta ( Kd approximately 30 nM ) . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| We conclude that ( 1 ) TRbeta 1 , like AR and ER , is subject to acetylation ; ( 2 ) the process of acetylation of TR requires thyroid hormone directed MAPK activity , but not serine phosphorylation of TR by MAPK , suggesting that the contribution of MAPK is upstream in the activation of the acetylase ; ( 3 ) the amino acid residue 128 142 region of the DBD of TR is important to thyroid hormone associated recruitment of p 300 and SRC 1 ; ( 4 ) acetylation of TR DBD mutants that is directed by T 4 appears to be associated with recruitment of p300 . . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| The SRG 3 coactivation of AR transactivation is achieved through the recruitment of coactivator SRC 1 , the protein level of which is upregulated by SRG 3 , providing another pathway of positive regulation . ^^^ Interestingly , SRG 3 coactivation of AR transactivation is fully functional in BRG1 / BRM deficient C33A cells and the AR / SRG3 / SRC 1 complex formed in vivo contains neither BRG 1 nor BRM protein , suggesting the possibility of an SRG 3 function independent of the SWI / SNF complex . ^^^ Importantly , the AR / SRG3 / SRC 1 complex occupies androgen response elements on the endogenous SRG 3 and PSA promoter in an androgen dependent manner in mouse prostate and LNCaP cells , respectively , inducing gene expression . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| To quantify their roles in the function of androgen receptor ( AR ) transcriptional activity in vivo , we generated a unique transgenic AR reporter mouse and analyzed the cell specific contributions of SRC 1 and TIF 2 to the activity of AR in mouse testis . ^^^ After characterization of these mice in terms of AR function , we further derived bigenic mice by crossing AR activity indicator mice with the SRC 1 / or TIF2+ / mutant mice . ^^^ Although SRC 1 concentrates in Sertoli cell nuclei in the absence of TIF 2 , nuclear SRC 1 is not able to rescue AR activity in the TIF 2 mutant background . ^^^ Interestingly , SRC 1 appears to negatively influence AR activity , thereby counterbalancing the TIF 2 stimulated AR activity . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Our goal was to evaluate the role of the androgen receptor coactivator SRC 1 in prostate cancer progression . ^^^ Reduction of SRC 1 expression significantly reduced growth and altered androgen receptor target gene regulation in both LNCaP and C 4 2 cell lines whereas it had no effect on the growth of the androgen receptor negative PC 3 and DU 145 prostate cancer cell lines . ^^^ Although the requirement for androgens and androgen receptor in the development of prostate cancer is well established , our study implicates enhanced androgen receptor activity through elevated expression of SRC 1 in the development of more aggressive disease in men with prostate cancer . . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation analyses showed that DHT increased the recruitment of AR and coactivators , such as SRC 1 and TIF 2 , to the promoter of the PSA gene , and that recruitment was greatly diminished in the presence of 5 micromol / L selenium . ^^^ |
|
| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| N CoR impaired the DHT induced N C interaction of AR , and the impaired interaction was dose dependently recovered by coexpression of SRC 1 and CBP . ^^^ Coexpression of SRC 1 or CBP released YFP N CoR or endogenous N CoR from incomplete foci and simultaneously recovered complete foci of AR green fluorescent protein . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| While all three coactivators ARA 70 , steroid receptor coactivator 1 , and RAC3 / ACTR can enhance androgen receptor ( AR ) transcriptional activity at 1 nM dihydrotestosterone , we here demonstrate that only ARA 70 can induce AR transcriptional activity > 30 fold in the presence of 10 nM 17beta estradiol ( E 2 ) , but not diethylstilbestrol . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Our studies also demonstrate that Tip 60 co immunoprecipitates with the full length AR in vitro and that , in our system , Tip 60 enhances transactivation to levels observed with the coactivators steroid receptor coactivator 1 , p 300 , and CREB binding protein . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Here we report that the C terminal region of p 160 coactivators glucocorticoid receptor interacting protein 1 ( GRIP 1 ) , steroid receptor coactivator 1 ( SRC 1a ) , and SRC 1e binds the N terminal AF 1 activation function of the androgen receptor ( AR ) , and p 160 coactivators can thereby enhance transcriptional activation by AR . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Here we report that a majority of recurrent prostate cancers express high levels of the androgen receptor and two nuclear receptor coactivators , transcriptional intermediary factor 2 and steroid receptor coactivator 1 . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To examine expression of androgen receptor ( AR ) , AR cofactors , estrogen ( E ) receptor alpha , E receptor beta , progesterone receptor , steroid receptor coactivator 1 , and aromatase in human luteinized granulosa cells collected during oocyte retrieval . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Knocking down SMRT and N CoR enhanced the recruitment of the coactivators steroid receptor coactivator 1 and p 300 by agonist bound AR and led to increased hyperacetylation of histone H 3 and H 4 , suggesting that the corepressors actively compete with coactivators for binding to agonist bound AR . ^^^ |
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| Interacting proteins: Q15788 and P10275 |
Pubmed |
SVM Score :0.0 |
| Expression changes of 10 mRNAs of interest ( AR , Rb , ARA 160 , ARA 24 , ARA 54 , ARA 55 , ARA 70 , BRCA 1 , F SRC 1 , and RAC 3 ) were analyzed simultaneously as AR and AR associated cofactors in 1 hybridization reaction . ^^^ |
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