| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.97801296 |
| Unlike agonist bound PR that interacts only with coactivators such as steroid receptor coactivator 1 ( SRC 1 ) , RU 486 bound PR binds to both coactivator SRC 1 and corepressor silencing mediator for retinoid and thyroid hormone receptor ( SMRT ) in vitro . 0.97801296^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.72891743 |
| Upon ligand treatment , progesterone receptor ( PR ) interacted preferentially with SRC 1 , which recruited CBP and significantly enhanced acetylation at K 5 of histone H 4 . 0.72891743^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Coexpression of CBP and SRC 1 stimulated ER and PR transcriptional activity in a synergistic manner and indicated that these two coactivators are not functional homologues . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Furthermore , PR transactivation was repressed by recruiting HD 1 into the PR DNA complex by fusing HD 1 to a PR ligand binding domain interacting portion of SRC 1 . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| CBP and SRC 1 interact and synergistically enhance transcriptional activation by the ER and PR . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In addition , we demonstrate that liganded PR is present in stable complexes containing SRC 1 and transcription intermediary factor 2 ( TIF 2 ) in vivo . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Steroid receptor coactivator 1 ( SRC 1 ) family members interact with steroid receptors , including estrogen receptor alpha ( ERalpha ) and progesterone receptor ( PR ) , to enhance ligand dependent transcription . ^^^ Treatment of animals with estrogen induced PR expression in the ERalpha expressing mammary epithelial cells in the absence of detectable SRC 1 and did not affect the segregated pattern of SRC 1 and ERalpha expression . ^^^ PR was neither expressed nor induced by estrogen treatment in stroma , despite the coexpression of ERalpha and SRC 1 . ^^^ These results suggest that SRC 1 is not necessary for ERalpha mediated induction of PR in mammary epithelial cells and is also not sufficient for PR induction in stromal cells expressing both ERalpha and SRC 1 . ^^^ Furthermore , the expression of SRC 1 in a subpopulation of mammary epithelial cells distinct from those expressing ERalpha or PR raises the possibility that SRC 1 has cell type specific functions other than simply to act as coactivator for ERalpha or PR in the mammary epithelium . . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The transcriptional activity of PR requires the involvement of coactivators such as steroid receptor coactivator 1 ( SRC 1 ) . ^^^ To dissect the role of SRC 1 in PR transactivation , we established an in vitro transcription system with chromatin templates , in which PR induced transcription in a ligand dependent and PRE dependent manner . ^^^ With this system , the ability of purified SRC 1 to act as a coactivator of PR was examined . ^^^ SRC 1 potentiated transcription by ligand activated PR , whereas it had no effect on transcription in the absence of ligands . ^^^ As SRC 1 possesses intrinsic histone acetyltransferase activity , we tested the role of acetylation in PR mediated transcription by using a histone deacetylase inhibitor , trichostatin A ( TSA ) . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Here we provide evidence that the hormone occupied PR forms a multisubunit receptor coactivator complex containing two previously described coactivators , CREB binding protein ( CBP ) and steroid receptor coactivator 1 ( SRC 1 , a member of the p 160 family of coactivators ) , in nuclear extracts of human breast tumor T47D cells . ^^^ Furthermore , depletion of SRC 1 / p160 by immunoprecipitation from these transcriptional extracts also significantly impaired PR mediated RNA synthesis from a naked PRE linked DNA template . ^^^ Most importantly , we noted that binding of E1A to CBP prevented the assembly of a coactivation complex containing PR , CBP , and SRC 1 / p160 , presumably by disrupting the interaction between CBP and SRC 1 / p160 . ^^^ Collectively , our results support the hypothesis that the assembly of a multisubunit coactivation complex containing PR , CBP , and SRC 1 / p160 is a critical regulatory step during hormone dependent gene activation by PR and that the fully assembled complex has the ability to control transcription through mechanisms that are independent of the histone modifying activities of its component coactivators . . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| We show here , by yeast and mammalian two hybrid analyses and by pull down experiments , that JAB 1 also interacts with both the progesterone receptor ( PR ) and the steroid receptor coactivator 1 ( SRC 1 ) and that it stabilizes PR SRC 1 complexes . ^^^ This occurs without any modification of PR or SRC 1 concentration . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Employing a cell free chromatin transcription system that recapitulates progesterone receptor ( PR ) mediated transcription in vivo , we have investigated further the coactivator functions of steroid receptor coactivator 1 ( SRC 1 ) in terms of its functional domains as well as cooperation with other coactivators in PR transactivation . ^^^ By analyzing wild type and mutant SRC 1 with liganded PR in the chromatin transcription system in vitro , the basic helix loop helix / Per Arnt Sim domain , the p 300 binding domain , and the carboxyl terminal region ( containing the PR binding site ) of SRC 1 were shown to be important for PR transactivation . ^^^ Although in context of a synthetic promoter its histone acetyltransferase activity was nonessential for PR mediated transcription , SRC 1 was observed to act synergistically with p 300 to enhance PR transactivation from chromatin . ^^^ Further analysis of synergism between SRC 1 and p 300 revealed an obligatory `` sequential ' ' recruitment of SRC 1 and p 300 to liganded PR . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| This activity is comparable with that of the coactivator SRC 1 , but , in contrast , the interaction between EDD and PR does not appear to involve an LXXLL receptor binding motif . ^^^ |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Sumoylation was shown to increase PR SRC 1 interaction and to prolong SRC 1 retention in the nucleus . ^^^ Overexpression of SUMO 1 enhanced PR mediated gene transcription even in the presence of non sumoylated mutants of SRC 1 . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| To examine the sex steroid dependent growth mechanisms of the human endometrium , the expression of steroid receptor coactivators [ steroid receptor coactivator 1 ( SRC 1 ) and p300 / CREB binding protein ( p300 / CBP ) ] and corepressors ( nuclear receptor corepressor and silencing mediator for retinoid and thyroid hormone receptors ) was examined by immunohistochemistry , using 50 samples of normal endometria , and was compared with that of estrogen receptors ( ER ) , progesterone receptors ( PR ) , and proliferation marker Ki 67 . ^^^ Such change in the expression pattern of SRC 1 resembled that of ER , PR , and Ki 67 . ^^^ Complex formation between p300 / CBP and PR was noted in the secretory endometrium , whereas that between SRC 1 and PR was not apparent . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| We also show that the ligand dependent activities of ERalpha and progesterone receptor ( PR ) in yeast cells were strongly enhanced by the human p 160 protein steroid receptor coactivator ( SRC 1 ) , but not by CREB Binding Protein ( CBP ) or the p300 / CBP associated factor ( P / CAF ) . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Interestingly , RRL stimulated sequence specific binding by PR to target sites in chromatin and the concomitant recruitment of the steroid receptor coactivator 1 to the promoter . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| METHODS : Gene expression of SRC 1 , SRC 2 , SRC 3 , N CoR , SMRT , ERalpha , and PR was measured in 26 samples of normal endometrium and 30 primary endometrial carcinomas using real time RT PCR . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Inhibition of Cdk 2 activity abolishes progesterone dependent activation of PR target genes in part through inhibition of PR dependent recruitment of steroid receptor coactivator 1 ( SRC 1 ) and subsequent histone H 4 acetylation at the target promoter . ^^^ In vitro studies revealed that the interaction between SRC 1 and PR is dependent upon phosphorylation of SRC 1 . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Although PR is recruited to the MMTV promoter equivalently in the G 1 and S phases , recruitment of SRC 1 , SRC 3 , and , consequently , CBP is reduced in G 1 phase despite comparable expression levels of SRC 1 and SRC 3 . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Bigenic PRAI SRC 1 ( / ) mice revealed that SRC 1 modulates PR activity in the uterus in a cell specific fashion and is involved in PR gene activation in stroma and myometrium of the uterus in response to estrogen and progesterone . ^^^ In contrast , SRC 1 was involved in the down regulation of PR target gene expression in the luminal and glandular epithelial compartments of the uterus after chronic progesterone treatment . ^^^ Finally , we dissected the means by which SRC 1 dynamically regulates PR activity in each uterine cell compartment and demonstrated that it involves the differential ability of SRC 1 to modulate expression levels of distinct coactivators , corepressors , and PR in a cell specific fashion . . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| To investigate the functional interactions between PR and steroid receptor coactivators ( SRCs ) required for regulation of gene transcription in vivo , we crossed PR activity indicator ( PRAI ) mice with SRC 1 ( + / ) and SRC 3 ( + / ) mice to generate bigenic mice , PRAI SRC 1 ( / ) and PRAI SRC 3 ( / ) . ^^^ In the mammary gland , PR activity in the luminal epithelium of both wild type and SRC 1 ( / ) mice was induced by estrogen + progesterone treatment . ^^^ However , the increased PR activity was not detected in SRC 1 ( / ) mice . ^^^ SRC 3 is the primary coactivator for PR in breast and SRC 1 is the primary coactivator for PR in uterus . . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Estrogen independent cells were still estrogen responsive and PR , nuclear receptor corepressor ( N CoR ) and SMRT expression was increased whereas steroid receptor coactivator 1 ( SRC 1a ) and CBP related protein p 300 ( p 300 ) expression decreased . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Sexually dimorphic expression of ER alpha and PR was noted with predominance in females and males , respectively , EE up regulating SRC 1 in males and ER beta and PR in females . ^^^ Next , we similarly examined expression changes of ER alpha and beta , PR , and SRC 1 in animals exposed to MXC at 24 , 240 , and 1200 ppm , DINP at 4000 and 20 , 000 ppm , and GEN at 1000 ppm . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Using small interfering RNA , we determined that induction is dependent on the presence of PR , estrogen receptor and SRC 1 . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Additional studies revealed that PPM1D enhanced the intrinsic activity of p 160 coactivators such as steroid receptor coactivator 1 and promoted the interaction between PR and steroid receptor coactivator 1 in a mammalian two hybrid assay . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In this work we have determined the role of the 26S proteasome in the regulation of the content of progesterone receptors ( PR A and PR B ) , estrogen receptors ( ER alpha and ER beta ) , the coactivator SRC 1 and the corepressor SMRT in the rat brain during the estrous cycle . ^^^ The 26S proteasome inhibitor MG 132 was injected once into the lateral ventricle on proestrous day ; and 24h later , on estrous day we evaluated the content of PR and ER isoforms , SRC 1 and SMRT in the hypothalamus , the preoptic area and the hippocampus by Western blot . ^^^ A significant increase in the content of both PR isoforms , ER beta and SRC 1 was observed after the administration of MG 132 in the three studied cerebral regions . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Conversely , the MYST acetylase domain specifically enhanced SRC 1 coupling with PR NTD , through a hormone dependent mechanism . ^^^ Importantly , real time RT PCR analysis also revealed that HBO 1 enhanced SRC 1 coactivation of PR dependent transcription of human endogenous genes such as alpha 6 integrin and 11beta hydroxydehydrogenase 2 but not that of amphiregulin . ^^^ Immunofluorescence and confocal microscopy of human embryonic kidney PRB cells demonstrated that the hormone induces the colocalization of HBO 1 with PR SRC 1 complex into nuclear speckles characteristic of PR mediated chromatin remodeling . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Previously , our lab has shown that SRC 1 and CBP modulate estrogen receptor ( ER ) mediated induction of progestin receptor ( PR ) gene expression in the ventromedial nucleus of the hypothalamus ( VMN ) and hormone dependent sexual receptivity in female rats . ^^^ In the present experiments , the function of SRC 1 and CBP in distinct ER ( Exp . 1 ) and PR ( Exp . 2 ) dependent aspects of female sexual behavior was investigated . ^^^ In Exp . 2 , antisense to SRC 1 and CBP mRNA around the time of P administration reduced PR dependent ear wiggling and hopping and darting . ^^^ Taken together , these data suggest that SRC 1 and CBP modulate ER and PR action in brain and influence distinct aspects of hormone dependent sexual behaviors . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Absence of SRC 2 in PR positive uterine cells was shown to contribute to an early block in embryo implantation , a phenotype not shared by SRC 1 or 3 knockout mice . ^^^ Moreover , removal of SRC 1 in the PR ( Cre / + ) SRC 2 ( flox / flox ) mouse uterus resulted in the absence of a decidual response , confirming that uterine SRC 2 and 1 cooperate in P initiated transcriptional programs which lead to full decidualization . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| In contrast , SRC 1 , a coactivator for the progesterone receptor , inhibited both AR mediated transactivation and interaction between the N and C termini . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| One leptomeningeal specimen was positive for AIB 1 , SRC 1 , and progesterone receptor . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Bromo cyclic AMP induces phosphorylation of two sites in SRC 1 that facilitate ligand independent activation of the chicken progesterone receptor and are critical for functional cooperation between SRC 1 and CREB binding protein . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Unlike the steroid hormone receptor coactivator 1 ( SRC 1 ) , beta catenin showed a strong interaction with AR but not with other steroid hormone receptors such as estrogen receptor alpha , progesterone receptor beta , and glucocorticoid receptor . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| However , mutation of the two cAMP inducible SRC 1 phosphorylation sites important for cAMP activation of chicken progesterone receptor or all seven known SRC 1 phosphorylation sites did not specifically impair cAMP activation of ER alpha . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| Treatment of cultured human uterine smooth muscle cells ( UtSMC ) with TNF alpha significantly reduced mRNA for the coactivators , SRC 1 ( 42 % , P < 0 . 01 ) and 2 ( 47 % , P < 0 . 03 ) , and diminished the respective protein levels , but did not significantly alter the mRNAs encoding SRC 3 , CBP and the corepressors , NCoR and SMRT ; or progesterone receptor protein levels . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To examine expression of androgen receptor ( AR ) , AR cofactors , estrogen ( E ) receptor alpha , E receptor beta , progesterone receptor , steroid receptor coactivator 1 , and aromatase in human luteinized granulosa cells collected during oocyte retrieval . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| The tumoral ER alpha protein expression was significantly correlated with that of PgR ( r = 0 . 61 , p = 0 . 001 ) and NCoR ( r = 0 . 4 , p = 0 . 043 ) , whereas ER beta expression was associated with SRC 1 ( r = 0 . 68 , p < or = . 001 ) , TIF 2 ( r = 0 . 64 , p = 0 . 001 ) and NCoR ( r = 0 . 48 , p = 0 . 014 ) protein levels in malignant specimens . ^^^ |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06401 and Q15788 |
Pubmed |
SVM Score :0.0 |
| NA |
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