| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.79465578 |
| Accordingly , ERalpha dephosphorylation decreases its ligand independent interaction with SRC 1 and CBP in vitro . 0.79465578^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.99098951 |
| Whereas wild type ER showed interaction with SRC 1 only in the presence of estrogen , Y537A and Y537S ER showed moderate or full interaction in the absence of ligand , an interaction that was blocked by antiestrogen , and the magnitude of interaction was increased to or remained at 100 % upon estradiol treatment , implying that the ability of an ER to associate with SRC 1 is a good indicator of a transcriptionally active conformational state of the receptor . 0.99098951^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.60108402 |
| CONCLUSIONS : 1 ) FRET based coactivator association is a novel approach for characterizing nuclear receptor agonists or antagonists ; individual ligands display potencies that are predictive of in vivo effects and distinct profiles of maximal activity that are suggestive of alternative receptor conformations . 2 ) PPARgamma interacts with both CBP and SRC 1 ; transcriptional activation and coactivator association are AF 2 dependent . 3 ) Nuclear receptor LBDs have distinct affinities for individual coactivators ; thus , PPARgamma has a greater apparent affinity for CBP than for SRC 1 , whereas ERalpha interacts preferentially with SRC 1 but very weakly with CBP . . 0.60108402^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.94242807 |
| SRC 1 , AIB 1 , and RIP 140 interacted constitutively with the L536P ER , whereas TIF 1 and TIF 2 interacted only weakly in the absence of hormone . 0.94242807^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.55466822 |
| Enhancement of the interaction between SRC 1 and ER beta AF 1 is also observed in vivo in cells either treated with EGF or expressing activated Ras . 0.55466822^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.67290845 |
| Thus , in oestrogenic conditions SRC 1 preferentially binds to the ER which effectively sequesters it thereby reducing enhancer activity , but in antioestrogenic media the cofactor is released from the ER and is therefore available to activate the ERBB 2 enhancer . . 0.67290845^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :1.0966839 |
| Basal and estradiol ( E 2 ) dependent interactions between the ERalpha ligand binding domain ( LBD ) and SRC 1 , TIF 2 or RAC 3 were observed in HeLa and HepG 2 cells . 1.0966839^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :1.2724379 |
| Competition experiments of the complex between ERalpha and fluorescently labelled PGC 1 91 408 with unlabelled SRC 1 570 780 showed that PGC 1 91 408 was an efficient competitor of SRC 1 570 780 , while the inverse was not true , underscoring their distinct modes of binding . 1.2724379^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.55707465 |
| We demonstrate that both FTI 277 and GGTI 298 increase the association of steroid receptor coactivator 1 with ER alpha and FTI 277 decreases the association of ER alpha with the histone deacetylase 1 , a known transcriptional repressor . 0.55707465^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Coexpression of SRC 1 reversed the ability of the estrogen receptor to squelch activation by hPR . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| CBP and SRC 1 interact and synergistically enhance transcriptional activation by the ER and PR . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Up regulation of the steroid receptor coactivator SRC 1 did not seem to mediate the process of enhanced ER induced transcription . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| The expression of SRC 1 and TIF 2 is significantly related to progesterone but not to estrogen receptor expression . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Dual FRAP experiments show that fluorescent ER and SRC 1 exhibit similar dynamics only in the presence of E 2 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| SRC 1 , the p 160 steroid receptor coactivator family member , synergized with XBP 1S or XBP 1U to potentiate ERalpha activity . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| In endometrial hyperplasia , there was a significant correlation between the expression of ER and SRC 1 or p300 / CBP . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Using small interfering RNA , we determined that induction is dependent on the presence of PR , estrogen receptor and SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Multivariate regression analyses were used to assess the relation between untreated cross sectional and longitudinal blood pressure and polymorphisms at the estrogen receptor alpha ( ESR 1 ) , estrogen receptor beta ( ESR 2 ) , aromatase ( CYP19A1 ) , and nuclear receptor coactivator 1 ( NCOA 1 ) genes after adjustment for common risk factors . ^^^ RESULTS : In men , systolic blood pressure and pulse pressure ( systolic blood pressure minus diastolic blood pressure ) were associated with two polymorphisms in ESR 1 , while pulse pressure was also associated with variations in NCOA 1 and CYP19A1 . ^^^ Polymorphisms in ESR 1 , CYP19A1 , and NCOA 1 were associated with diastolic blood pressure in women . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Coexpression of CBP and SRC 1 stimulated ER and PR transcriptional activity in a synergistic manner and indicated that these two coactivators are not functional homologues . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| To explore a possible role of steroid receptor coactivators in transcriptional synergism between AF 1 and AF 2 , we expressed the amino terminal ( AF 1 containing ) and carboxyl terminal ( AF 2 containing ) regions of ER as separate polypeptides in mammalian cells , along with the steroid receptor coactivator 1 protein ( SRC 1 ) . ^^^ We demonstrate that SRC 1 , which has been shown to significantly increase ER transcriptional activity , enhanced the interaction , mediated by either E 2 or TOT , between the AF 1 containing and AF 2 containing regions of the ER . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , the coactivator SRC 1 up regulates mER beta transactivation both in the absence and presence of E 2 , and in vitro interaction between SRC 1 and the ER beta ligand binding domain is enhanced by E 2 . ^^^ Moreover , the ligand independent stimulatory effect of SRC 1 on ER beta transcriptional activity is abolished by ICI 182 , 780 , but not by OHT . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| To determine whether coactivators and corepressors have the capacity to modulate the relative agonist / antagonist activity of 4HT , ER dependent gene expression was measured in the absence or presence of expression vectors for SRC 1 ( steroid receptor coactivator 1 ) or SMRT ( silencing mediator of retinoic acid and thyroid hormone receptors ) . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We have analyzed several transcriptional steps that could be involved in the activation : the ability of these receptors 1 ) to interact with several coactivators ( steroid receptor coactivator 1 , SRC 1 ; transcription intermediary factor 1 , TIF 1 ; and estrogen receptor associated protein 140 , ERAP 140 ) and with members of the preinitiation complex [ TATA box binding protein ( TBP ) , transcription factor IIB ( TFIIB ) ] ; 2 ) to exhibit conformational changes revealed by proteolytic digest patterns similar to those observed for the wild type hormone occupied ER ; and 3 ) to bend estrogen response element containing DNA , which is thought to be one of the important phenomena triggering transcriptional activation . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| In addition , EM 652 blocks the E 2 dependent activation of ER alpha and ER beta by the steroid hormone receptor coactivator 1 as well as the in vitro interaction between SRC 1 and the ligand binding domains of both ERs . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Steroid receptor co activator ( SRC 1 ) is one of a number of transcriptional co activators that are capable of potentiating the activity of nuclear receptors including the oestrogen receptor ( ER ) . ^^^ Thus , SRC 1 exists as functionally distinct isoforms which are likely to play different roles in ER mediated transcription . . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Steroid receptor coactivator 1 ( SRC 1 ) appears to be a general coactivator for steroid receptors and rate limiting factor necessary for efficient ER transactivation . ^^^ There was no relationship between the levels of SRC 1 in these primary tumors and the proportion of tumor cells within the surgical samples , nor with ER status . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| The two cofactors for steroid receptors RIP 140 and SRC 1 do not seem to be specifically involved in the insulin induced ER alpha transactivation . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Steroid receptor coactivator 1 ( SRC 1 ) family members interact with steroid receptors , including estrogen receptor alpha ( ERalpha ) and progesterone receptor ( PR ) , to enhance ligand dependent transcription . ^^^ However , the expression of ERalpha and SRC 1 was found to be segregated in distinct subsets of cells within the epithelium of the estrogen responsive rat mammary gland . ^^^ This finding was in contrast to the finding for the stroma , where significant numbers of cells coexpressed ERalpha and SRC 1 . ^^^ Treatment of animals with estrogen induced PR expression in the ERalpha expressing mammary epithelial cells in the absence of detectable SRC 1 and did not affect the segregated pattern of SRC 1 and ERalpha expression . ^^^ PR was neither expressed nor induced by estrogen treatment in stroma , despite the coexpression of ERalpha and SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We also show that overexpression of the steroid receptor coactivator ( SRC 1 ) potentiates transactivation by genistein activated ER alpha and that coexpression of CBP ( the cAMP response element binding protein coactivator ) synergistically increases this signal . ^^^ In in vitro binding assays , we show that genistein , but not 4 hydroxytamoxifen , induces a direct interaction between radiolabeled ER alpha and a GST SRC 1 fusion protein . ^^^ More importantly , coincubation with genistein and 4 hydroxytamoxifen or genistein treatment following preincubation of the ER with 4 hydroxytamoxifen also resulted in a strong physical interaction with SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We have analyzed the contribution of acidic AF 2 residues to the process of ER coactivation by the steroid receptor coactivator , SRC 1 . ^^^ In HeLa cells , SRC 1 coexpression was found to restore transcriptional potency to otherwise inert complexes of wild type ER and 4 hydroxyestratrien 17beta ol . ^^^ SRC 1 coexpression also enhanced transcriptional activity of reporter genes induced by an ER mutant with neutral replacements to acidic AF 2 residues , in response to E 2 or 4 hydroxyestratrien 17beta ol . ^^^ Changes in the structure of the ligand or substitutions to AF 2 residues in the estrogen receptor make independent contributions to coactivator sensitivity by SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| By two hybrid assay in yeast , the interactions of estrogen receptor with RIP 140 , SRC 1 , TIF 1 , and TIF 2 were detected and they were completely dependent on the presence of estrogen . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| In affinity interaction assays for proteins that associate specifically with the hormone binding domain of these receptors , we demonstrate that the steroid receptor coactivator SRC 1 interacts in an estrogen dependent manner with ERalpha and ERbeta 1 , but not with ERbeta 2 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| However , TERP 1 may compete with ER for binding sites of receptor cofactors because steroid receptor coactivator 1 ( SRC 1 ) rescued the inhibitory actions of TERP 1 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| EM 652 inhibits Ras induced transcriptional activity of ER alpha and ER beta and blocks SRC 1 stimulated activity of the two receptors . ^^^ EM 652 was also found to block the recruitment of SRC 1 at AF 1 of ER beta , this ligand independent activation of AF 1 being closely related to phosphorylation of the steroid receptors by protein kinase . ^^^ Most importantly , the antiestrogen hydroxytamoxifen has no inhibitory effect on the SRC 1 induced ER beta activity while the pure antiestrogen EM 652 completely abolishes this effect , thus strengthening the need to use pure antiestrogens in breast cancer therapy in order to control all known aspects of ER regulated gene expression . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| This binding did not interfere with either the ERalpha dimerization or the interaction between hERalpha and its coactivator SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We used a fluorescent ligand for ER , tetrahydrochrysene ketone , to monitor the rates of ligand dissociation from ERalpha and ERbeta , and to see how this process is affected by the p 160 class coactivator , steroid receptor coactivator 1 ( SRC 1 ) . ^^^ We used a 15 amino acid peptide corresponding to the second nuclear receptor box LXXLL motif in SRC 1 ( NR 2 peptide ) , which is known to interact with the ER ligand binding domain , a mutant peptide with an LXXAL sequence ( NR 2A peptide ) , and a 203 amino acid fragment of SRC 1 , termed the nuclear receptor domain ( SRC 1 NRD ) , embodying all three of the internal NR boxes of this protein . ^^^ Both the NR 2 peptide and the SRC 1 NRD fragment markedly slow the rate of dissociation of the agonist ligands tetrahydrochrysene ketone , estradiol , and diethylstilbestrol , increasing the half life of the ER agonist complex by up to 50 to 60 fold . ^^^ The SRC 1 NRD has much higher potency in retarding ligand dissociation than does the NR 2 peptide ; it is maximally effective at 30 nM , and it appears to bind with the stoichiometry of one SRC 1 NRD per ER dimer . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| ASC 2 also interacted with other nuclear receptors , including retinoic acid receptor , thyroid hormone receptor , estrogen receptor alpha , and glucocorticoid receptor , basal factors TFIIA and TBP , and transcription integrators CBP / p300 and SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| This recruitment is in direct contrast to the recruitment of SRC 1 to the estrogen receptor , which requires interaction with the ligand binding domain . . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| To obtain some clue to these roles , we screened the expression levels of ER alpha , ER beta , coactivators ( SRC 1 , TIF 2 , AIB 1 , CBP , and P / CAF ) and corepressors ( N CoR and SMRT ) in 6 normal mammary glands , 6 intraductal carcinomas , 22 invasive ductal carcinomas , and 7 breast cancer cell lines using a multiplex reverse transcription PCR . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Because TERP 1 contains a dimerization domain and part of the coactivator binding pocket , we hypothesized that it modulates ER function by direct interactions with full length ER or the steroid receptor coactivator , SRC 1 . ^^^ TERP 1 also binds SRC 1 , and increasing levels of SRC 1 decrease the TERP 1 ERalpha interactions , in agreement with the rescue of TERP 1 suppressed ERalpha transcriptional activity by SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that both peroxisome proliferator activated receptor binding protein ( PBP ) and steroid receptor coactivator 1 ( SRC 1 ) are required for ligand dependent transcription of transiently transfected and chromosomally integrated reporter genes by the estrogen receptor ( ER ) and retinoic acid receptor ( RAR ) . ^^^ These findings suggest that ligand dependent transcriptional activities of the RAR and ER require concurrent or sequential recruitment of SRC 1 and PBP containing coactivator complexes . . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Experiments performed with bioluminescent derivatives of ERalpha and steroid receptor coactivator 1 ( SRC 1 ) demonstrated both proteins colocalize to the same NM bound foci in response to E 2 but not the antagonists tested . ^^^ Collectively , our data suggest that ERalpha transcription function is dependent upon dynamic early events including intranuclear rearrangement , NM association , and SRC 1 interactions . . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Using a mammalian two hybrid assay , we show that the thyroid hormone receptor beta ( TRbeta ) and estrogen receptor beta ( ERbeta ) have different LXXLL motif preferences for interactions with SRC 1 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| However , unlike estrogens , none of these xenobiotics seemed to be able to induce ER / SRC 1 interactions , most likely because the conformation of the activated receptor would not allow direct contacts with this coactivator . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Constitutive uterine mRNA expression of switch protein for antagonist ( SPA ) , SRC 1 , GRIP 1 , RAC 3 , RIP 140 , and p 300 mRNAs was observed in control uteri , and treatment with ER ligands did not alter coactivator mRNA levels . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Cotransfection of PTalpha or SRC 1 with increasing amounts of REA , as well as competitive glutathione S transferase pulldown and mammalian two hybrid studies , show that REA competes with PTalpha ( or SRC 1 ) for regulation of ER transcriptional activity and suppresses the ER stimulation by PTalpha or SRC 1 , indicating that REA can function as an anticoactivator in cells . ^^^ Our data support a model in which PTalpha , which does not interact with ER , selectively enhances the transcriptional activity of the ER but not that of other nuclear receptors by recruiting the repressive REA protein away from ER , thereby allowing effective coactivation of ER with SRC 1 or other coregulators . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| REA and the coactivator SRC 1 were involved in a functional competition for regulation of ER transcriptional activity , which we show results from competition between these two coregulators for interaction with ER . ^^^ Rather , this sequence was required for the competitive binding of REA and SRC 1 to ER and thus for optimal repression of ER activity . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that transcriptional activation by the estrogen receptor ( ER ) requires functional BRG 1 and that the coactivation of estrogen signaling by either SRC 1 or CBP is BRG 1 dependent . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| To investigate the molecular mechanisms underlying the ER subtype selective actions of these compounds , we have determined the conformational changes induced in ERalpha and ERbeta by these ligands using protease digestion sensitivity , and we have tested the ability of these ligands to promote the recruitment of representatives of the three SRC / p160 coactivator protein family members ( SRC 1 , GRIP 1 , ACTR , respectively ) to ERalpha and ERbeta using yeast two hybrid and glutathione S transferase ( GST ) pull down assays . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| In addition , the D351G ER retains the ability to bind SRC 1 in the presence of E 2 , thus D351G ER AF 2 activity has not been compromised . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We show that in contrast to SRC 1 , direct binding of CBP to the estrogen receptor is weak , suggesting that SRC 1 functions primarily as an adaptor to recruit CBP and p 300 . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We describe a ligand that binds to both receptors , but enhances only ER beta interaction with SRC 1 and SRC 3 while exhibiting little effect on the ER alpha interaction with these proteins . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We previously identified a conserved potential alpha helical structure within the AF 1 functional core , and by evaluating point mutants of human ERalpha ( hERalpha ) within this region , we show that in transfection experiments this structure is required for synergism between SRC 1 and hERalpha . ^^^ This interaction of SRC 1 with the AF 1 alpha helical core is essential for both E 2 and OHT induced ERalpha activity . . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Unlike the steroid hormone receptor coactivator 1 ( SRC 1 ) , beta catenin showed a strong interaction with AR but not with other steroid hormone receptors such as estrogen receptor alpha , progesterone receptor beta , and glucocorticoid receptor . ^^^ |
|
| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Two nuclear receptor coactivators , steroid receptor coactivator 1 ( SRC 1 ) and cAMP response element binding protein binding protein ( CBP ) , have been shown to act in concert to enhance ER activity in vitro . ^^^ Reduction of SRC 1 and CBP protein in brain disrupted ER mediated activation of the behaviorally relevant progestin receptor gene . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| However there is no information on the consequences of reducing SRC 1 , TIF 2 , or SRA expression , singly or in combination , on ERalpha transcriptional activity . ^^^ Furthermore , treatment of cells with combinations of SRA , SRC 1 , and TIF 2 asODNs reduced ERalpha transcriptional activity to an extent greater than individual asODN treatment alone , suggesting that these coactivators cooperate , in at least an additive fashion , to activate ERalpha dependent target gene expression . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Employment of the human estrogen receptor beta ligand binding domain and co activator SRC 1 nuclear receptor binding domain for the construction of a yeast two hybrid detection system for endocrine disrupters . ^^^ We constructed two hybrid systems that co express the Gal4p DNA binding domain / ligand binding domain of human estrogen receptor ( hER ) alpha or beta and the Gal4p transactivation domain / nuclear receptor binding domain of co activator SRC 1 , TIF 2 , or AIB 1 in Saccharomyces cerevisiae with a chromosome integrated lacZ reporter gene under the control of Gal4p binding sites . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Furthermore , not only AR but also the glucocorticoid receptor YFP , ER alpha GFP , and YFP tagged SRC 1 , TIF 2 , and CBP were found to be accumulated in identical spots in the presence of ligand . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Although PRMT 2 was found to interact with two other coactivators , the steroid receptor coactivator 1 ( SRC 1 ) and the peroxisome proliferator activated receptor interacting protein ( PRIP ) , no synergistic enhancement of ERalpha transcriptional activity was observed when PRMT 2 was coexpressed with either PRIP or SRC 1 . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Collectively , the findings clearly implicate dual regulation of ERalpha dependent function by SRC 1 and SRC 2 in the intact female brain . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Our coactivator interaction assay utilizes time resolved fluorescence technology to assess the binding of the 10 NR boxes derived from the three known p 160 coactivators ( SRC 1 , 2 , 3 ) to the ER subtypes in the presence of each ligand . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Reproductive tracts were collected , weighed , and examined for changes in histomorphology and expression of ER and nuclear receptor co regulators ( SRC 1 , p 300 , CARM 1 , GRIP 1 , SPA , REA and Uba 3 ) . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| In addition to DNA binding , the interaction of both ER subtypes with the Alexa 488 labeled SRC 1 coactivator fragment was investigated by fluorescence anisotropy . ^^^ The interactions of human estrogen receptor subtypes ERalpha and ERbeta with DNA and a 210 amino acid residue fragment of the coactivator protein SRC 1 bearing three nuclear receptor interaction motifs were investigated quantitatively using fluorescence anisotropy in the presence of agonist and antagonist ligands . ^^^ Moreover , estrone and genistein exhibited subtype specificity in that they induced SRC 1 recruitment to ERbeta with much higher efficiency than in the case of ERalpha . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Furthermore , IA R bound ERalpha L384M and wild type ERbeta displayed enhanced interactions with the nuclear receptor interaction domains of the coactivators SRC 1 and GRIP 1 . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We then demonstrate that E 2 enhances interactions between ERalpha and the RIDs of SRC 1 and SRC 3 , whereas the interaction between ERalpha with the SRC 2 RID is ligand independent . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Replacement of either TRAP 220 LXXLL motif with the corresponding 13 amino acids of SRC 1 LXM2 strongly enhanced the interaction of the TRAP 220 NID with the estrogen receptor . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| However , mutation of the two cAMP inducible SRC 1 phosphorylation sites important for cAMP activation of chicken progesterone receptor or all seven known SRC 1 phosphorylation sites did not specifically impair cAMP activation of ER alpha . ^^^ Our data suggest that cAMP activation of ER alpha transcriptional activity is associated with receptor instead of SRC 1 phosphorylation . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| To examine the sex steroid dependent growth mechanisms of the human endometrium , the expression of steroid receptor coactivators [ steroid receptor coactivator 1 ( SRC 1 ) and p300 / CREB binding protein ( p300 / CBP ) ] and corepressors ( nuclear receptor corepressor and silencing mediator for retinoid and thyroid hormone receptors ) was examined by immunohistochemistry , using 50 samples of normal endometria , and was compared with that of estrogen receptors ( ER ) , progesterone receptors ( PR ) , and proliferation marker Ki 67 . ^^^ Such change in the expression pattern of SRC 1 resembled that of ER , PR , and Ki 67 . ^^^ Binding of SRC 1 to ER was observed in the proliferative ( but not in the secretory ) endometrium . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the overexpression of the steroid hormone receptor coactivator 1 ( SRC 1 ) resulted in preferential transcriptional enhancement by ERbeta as well as coexpressed ERalpha and ERbeta , whereas SRC 2 overexpression appeared to preferentially enhance ERalpha transactivation . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| The tumoral ER alpha protein expression was significantly correlated with that of PgR ( r = 0 . 61 , p = 0 . 001 ) and NCoR ( r = 0 . 4 , p = 0 . 043 ) , whereas ER beta expression was associated with SRC 1 ( r = 0 . 68 , p < or = . 001 ) , TIF 2 ( r = 0 . 64 , p = 0 . 001 ) and NCoR ( r = 0 . 48 , p = 0 . 014 ) protein levels in malignant specimens . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Further , 4EM mediated transcription in ER alpha , like estrogen , was enhanced in the presence of coactivators , steroid receptor coactivator 1 ( SRC 1 ) , CREB binding proteins ( CBP ) , and E 6 associated protein ( E 6 AP ) . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| The mutations impaired the interaction of the ER ligand binding domain with the SRC 1 receptor interacting domain in a mammalian two hybrid system . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Transcriptional activation by estrogen receptor ( ERalpha ) and steroid receptor coactivator ( SRC 1 ) involves distinct mechanisms in yeast and mammalian cells . ^^^ We also show that the ligand dependent activities of ERalpha and progesterone receptor ( PR ) in yeast cells were strongly enhanced by the human p 160 protein steroid receptor coactivator ( SRC 1 ) , but not by CREB Binding Protein ( CBP ) or the p300 / CBP associated factor ( P / CAF ) . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| The selective estrogen receptor modulator tamoxifen acted as a weak agonist of ERalpha mediated gene expression and this weak activity was potentiated by SRC 1 . ^^^ In contrast to previously reported yeast based ERalpha transactivation systems , the system reported here in which SRC 1 functions as a bona fide coactivator should permit a more thorough dissection of the factors involved in ERalpha mediated transcriptional activation . . ^^^ Potentiation of human estrogen receptor alpha mediated gene expression by steroid receptor coactivator 1 ( SRC 1 ) in Saccharomyces cerevisiae . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Reciprocal inhibition between Erg and ERalpha was not alleviated by overexpressing CBP , SRC 1 or RIP 140 , three nuclear coactivator proteins . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Here , we examined the effects that different ERalpha ligands have on coactivator protein steady state levels and demonstrate that the selective ER modulators ( SERMs ) 4 hydroxytamoxifen ( 4HT ) and raloxifene are able to elevate SRC 1 and SRC 3 protein levels . ^^^ Selective estrogen receptor modulators 4 hydroxytamoxifen and raloxifene impact the stability and function of SRC 1 and SRC 3 coactivator proteins . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| SRC 1 and SMRT were identified at the ER ERE complex , and interactions between ER isoforms and coregulatory proteins were determined using immunoprecipitation . ^^^ Both ER alpha and ER beta preferentially bound SRC 1 in the presence of beta estradiol . ^^^ Differential recruitment of SRC 1 and SMRT by ER alpha and ER beta in the presence of beta estradiol and 4 OHT may be central to the response of the tumor to endocrine treatment . . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| METHODS : Gene expression of SRC 1 , SRC 2 , SRC 3 , N CoR , SMRT , ERalpha , and PR was measured in 26 samples of normal endometrium and 30 primary endometrial carcinomas using real time RT PCR . ^^^ In the normal endometrium , SRC 1 mRNA expression was positively correlated with ERalpha protein expression and SRC 3 mRNA expression was positively correlated with patient age . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Inverse relationship between ER beta and SRC 1 predicts outcome in endocrine resistant breast cancer . ^^^ To determine isoform specific expression of ER and coexpression with activator proteins , we examined the expression and localisation of ER alpha , ER beta and the coactivator protein steroid receptor coactivator 1 ( SRC 1 ) by immunohistochemistry and immunofluorescence in a cohort of human breast cancer patients ( n=150 ) . ^^^ Protein expression of ER beta and SRC 1 was inversely associated ( P=0 . 0001 ) . ^^^ The association of ER beta protein expression with increased DFS and its inverse relationship with SRC 1 suggests a role for these proteins in predicting outcome in breast cancer . . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| CHO K 1 cells transfected with ERalpha or ERbeta show ERE sequence dependent differences in the functional interaction of ERalpha and ERbeta with coactivators steroid receptor coactivator 1 ( SRC 1 ) , SRC 2 ( glucocorticoid receptor interacting protein 1 ( GRIP 1 ) ) , SRC 3 amplified in breast cancer 1 ( AIB 1 ) and ACTR , cyclic AMP binding protein ( CBP ) , and steroid receptor RNA activator ( SRA ) , corepressors nuclear receptor co repressor ( NCoR ) and silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) , and secondary coactivators coactivator associated arginine methyltransferase 1 ( CARM 1 ) and protein arginine methyltransferase 1 ( PRMT 1 ) . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Src effects on ER phosphorylation and SRC 1 activity both contributed to tamoxifen agonist action on ER dependent gene expression in Ishikawa cells . ^^^ Taken together , these data demonstrate that src kinase potentiates tamoxifen agonist action through serine 167 dependent stabilization of ER promoter interaction and through elevation of SRC 1 and cAMP response element binding protein binding protein coactivation of ER . . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Ligand dependent estrogenic responses in NSCLC cells are probably generated via ERbeta and the p 160 coactivator GRIP1 / TIF2 , because expression of these proteins was detected , but not full length ERalpha or the p 160 coactivator SRC 1 . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We additionally analyzed the participation of the coactivators SRC 1 and cAMP response element binding protein ( CREB ) binding protein ( CBP ) in FSH evoked estrogen receptor ( ER ) dependent transactivation ; we found that CBP but not SRC 1 potentiated FSH induced transcriptional activation of both ER sensitive reporters , being this effect stronger on the ERE VitA 2 TK CAT than on the 3X ERE TAT Luc reporter . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We conclude that ( 1 ) TRbeta 1 , like AR and ER , is subject to acetylation ; ( 2 ) the process of acetylation of TR requires thyroid hormone directed MAPK activity , but not serine phosphorylation of TR by MAPK , suggesting that the contribution of MAPK is upstream in the activation of the acetylase ; ( 3 ) the amino acid residue 128 142 region of the DBD of TR is important to thyroid hormone associated recruitment of p 300 and SRC 1 ; ( 4 ) acetylation of TR DBD mutants that is directed by T 4 appears to be associated with recruitment of p300 . . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| The aim of the study was to test the hypothesis that expression of retinoid receptors ( RARalpha , RARbeta , RARgamma ) , rexinoid receptors ( RXRalpha , RXRbeta ) , thyroid hormone receptors ( TRalpha , TRbeta ) , estrogen receptors ( ERalpha , ERbeta ) , nuclear receptor coregulators ( N CoR , SRC 1 , SMRT ) , and in addition type 1 iodothyronine 5 ' deiodinase ( 5 ' DI ) , EGFR and erb B2 / neu would be different in mammary postlactating tissue in comparison with that of nonlactating mammary gland . ^^^ Using RT PCR , we have shown that expression of RARalpha , RXRalpha , TRalpha , ERalpha , ERbeta , N CoR , SRC 1 , SMRT and EGFR in rat was significantly increased in postlactating mammary gland when compared to that of nonlactating mammary tissue . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| The aims of this study were to compare the expression of the SRC 1 , SRC 2 , and SRC 3 coactivators between fibroids and normal myometrium in pure populations of cultured smooth muscle cells ( SMC ) and microvascular endothelial cells ( MEC ) , and also between both cell types , and to identify any relationship between the SRC expression profiles and the known ER status of the SMC and MEC samples examined in this study . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Sexually dimorphic expression of ER alpha and PR was noted with predominance in females and males , respectively , EE up regulating SRC 1 in males and ER beta and PR in females . ^^^ Next , we similarly examined expression changes of ER alpha and beta , PR , and SRC 1 in animals exposed to MXC at 24 , 240 , and 1200 ppm , DINP at 4000 and 20 , 000 ppm , and GEN at 1000 ppm . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation assays further demonstrate that MUC 1 ( 1 ) associates with ERalpha complexes on estrogen responsive promoters , ( 2 ) enhances ERalpha promoter occupancy , and ( 3 ) increases recruitment of the p 160 coactivators SRC 1 and GRIP 1 . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| A combination of the full length human estrogen receptor alpha with the nuclear receptor binding domain of co activator steroid receptor co activator 1 ( SRC 1 ) or transcriptional intermediate factor 2 ( TIF 2 ) was most effective for estrogen dependent induction of the chromosome integrated UAS ( GAL ) CYC 1 ( p ) lacZ reporter construct among the two hybrid systems so far tested . . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| In this work we have determined the role of the 26S proteasome in the regulation of the content of progesterone receptors ( PR A and PR B ) , estrogen receptors ( ER alpha and ER beta ) , the coactivator SRC 1 and the corepressor SMRT in the rat brain during the estrous cycle . ^^^ The 26S proteasome inhibitor MG 132 was injected once into the lateral ventricle on proestrous day ; and 24h later , on estrous day we evaluated the content of PR and ER isoforms , SRC 1 and SMRT in the hypothalamus , the preoptic area and the hippocampus by Western blot . ^^^ A significant increase in the content of both PR isoforms , ER beta and SRC 1 was observed after the administration of MG 132 in the three studied cerebral regions . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Herein , the molecular mechanisms involved in ER subtype selective interactions of these compounds as assessed by their effects upon both ERalpha and ERbeta structural conformation and their ability to induce recruitment of steroid receptor coactivator 1 ( SRC 1 ) to ERalpha were investigated . ^^^ In addition , these compounds had the ability to recruit SRC 1 to the ligand binding domain of ERalpha similar to E ( 2 ) . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation analysis showed that E ( 2 ) induced recruitment of C / EBPbeta , ERalpha , SRC 1 , p 300 , pCAF , TFIIB , and Pol 2 , with no change in Sp1 / Sp3 . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Previously , our lab has shown that SRC 1 and CBP modulate estrogen receptor ( ER ) mediated induction of progestin receptor ( PR ) gene expression in the ventromedial nucleus of the hypothalamus ( VMN ) and hormone dependent sexual receptivity in female rats . ^^^ In the present experiments , the function of SRC 1 and CBP in distinct ER ( Exp . 1 ) and PR ( Exp . 2 ) dependent aspects of female sexual behavior was investigated . ^^^ In Exp . 1 , infusion of antisense oligodeoxynucleotides to SRC 1 and CBP mRNA into the VMN decreased lordosis intensity in rats treated with E alone , suggesting that these coactivators modulate ER mediated female sexual behavior . ^^^ Taken together , these data suggest that SRC 1 and CBP modulate ER and PR action in brain and influence distinct aspects of hormone dependent sexual behaviors . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Strikingly , the literature shows that in vivo variations at residues in the new site are linked to androgen resistance and leukemia , and our own targeted mutations to this site lower but do not eradicate transcriptional activation by estrogen receptor alpha ( ERalpha ) , with reduced ligand binding affinity and SRC 1 interaction . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| AF 2 activity and recruitment of steroid receptor coactivator 1 to the estrogen receptor depend on a lysine residue conserved in nuclear receptors . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Furthermore , hormone independent interaction of ERalpha and ERbeta mutant receptors with the steroid receptor coactivator 1 was abrogated by these antiestrogens . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We have shown that expression of steroid receptor coactivator 1 alpha ( SRC 1alpha ) and receptor interacting protein 140 ( RIP 140 ) have no effect on the capacity of the ERalpha to modulate NFkappaB reporter gene activity in HeLa cells . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Its competitive reversal of steroid receptor coactivator 1 enhancement of ER activity and its direct interaction with liganded ER suggest that it may play an important role in determining the sensitivity of estrogen target cells , including breast cancer cells , to antiestrogens and estrogens . . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Studies with live cells demonstrate that agonist and antagonist rapidly ( within minutes ) modulate the subnuclear dynamics of estrogen receptor alpha ( ER ) and steroid receptor coactivator 1 ( SRC 1 ) . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Forskolin induced activation of ER was enhanced by cotransfection of steroid receptor coactivator 1 and was inhibited by the repressor of ER action , suggesting that cAMP does not alter the normal interactions between ER and cofactors . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| This binding blocks ER from association with the LXXLL motif of its coactivator , steroid receptor coactivator 1 , and thus represses ER effectively from carrying out transcription that regulates cell growth . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We targeted ER to gene amplified chromosome arms containing large numbers of lac operator sites either directly , through a lac repressor ER fusion protein ( lac rep ER ) , or indirectly , by fusing lac repressor with the ER interaction domain of the coactivator steroid receptor coactivator 1 . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Both ERalpha and ERbeta exhibited strong preferences for coactivator peptides corresponding to steroid receptor coactivator 1 and PPARgamma coactivor 1 vs . peroxisome proliferator activated receptor interacting protein and cAMP response element binding protein binding protein . 17beta Estradiol acted as a nonselective agonist for ERalpha and ERbeta . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| Two different color mutants of green fluorescent protein were joined by a tandem fusion domain composed of LXXLL ( L = leucine , 10 = any amino acid ) motif from the nuclear receptor box 2 of steroid receptor coactivator 1 , a flexible linker sequence , and the estrogen receptor alpha ligand binding domain ( ERalpha LBD ) . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| In addition , in endometrial cancer Ishikawa cells overexpressing steroid receptor coactivator 1 , 4 hydroxytamoxifen displayed full agonist activity and stimulated ER alpha mediated transcription without inducing receptor degradation . ^^^ |
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| Interacting proteins: Q15788 and P03372 |
Pubmed |
SVM Score :0.0 |
| We also show estrogen and AP 1 dependent recruitment of ER , steroid receptor coactivator 1 , and p 300 to the promoter of these genes by chromatin immunoprecipitation . ^^^ |
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