| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Cloning of the novel human myeloid cell specific C / EBP epsilon transcription factor . ^^^ By using a reverse transcription PCR based approach and a set of primers specific for the DNA binding domains of highly homologous members of the C / EBP family of transcriptional regulators , we have cloned a novel human gene encoding a member of the C / EBP gene family , identified as the human homolog of CRP 1 , C / EBP epsilon . ^^^ A 1 . 2 kb cDNA encoding full length human C / EBP epsilon was cloned from a promyelocyte late myeloblast derived lambda gt 11 library . ^^^ Primer extension analysis of C / EBP epsilon mRNA detected a single major transcription start site approximately 200 bp upstream of the start codon . ^^^ Western blot and immunoprecipitation studies using rabbit anti C / EBP epsilon antibodies raised against the N terminal portion of C / EBP epsilon ( amino acids 1 to 115 ) showed that C / EBP epsilon is a 32 kDa nuclear phosphoprotein . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| A novel , myeloid transcription factor , C / EBP epsilon , is upregulated during granulocytic , but not monocytic , differentiation . ^^^ Human C / EBP epsilon is a newly cloned CCAAT / enhancer binding transcription factor . ^^^ To elucidate the range of expression of C / EBP epsilon , we used reverse transcription polymerase chain reaction ( RT PCR ) analysis and examined its expression in 28 hematopoietic and 14 nonhematopoietic cell lines , 16 fresh myeloid leukemia samples , and normal human hematopoietic stem cells and their mature progeny . ^^^ Prominent expression of C / EBP epsilon mRNA occurred in the late myeloblastic and promyelocytic cell lines ( NB 4 , HL 60 , GFD 8 ) , the myelomonoblastic cell lines ( U 937 and THP 1 ) , the early myeloblast cell lines ( ML 1 , KCL 22 , MDS 92 ) , and the T cell lymphoblastic leukemia cell lines CEM and HSB 2 . ^^^ For the acute promyelocytic leukemia cell line NB 4 , C / EBP epsilon was the only C / EBP family member that was easily detected by RT PCR . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| CCAAT / enhancer binding protein epsilon ( C / EBP epsilon ) is preferentially expressed in granulocytes and lymphoid cells . ^^^ Mice with a null mutation in C / EBP epsilon develop normally and are fertile but fail to generate functional neutrophils and eosinophils . ^^^ Thus , C / EBP epsilon is essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells . . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Modulation of mRNA expression of a novel human myeloid selective CCAAT / enhancer binding protein gene ( C / EBP epsilon ) . ^^^ Human C / EBP epsilon is a newly cloned gene coding for a CCAAT / enhancer binding protein that may be involved in the regulation of myeloid differentiation . ^^^ Our studies showed that levels of C / EBP epsilon mRNA were markedly increased in NB 4 cells ( promyelocytic leukemia line ) , because they were induced by 9 cis retinoic acid ( 9 cis RA ) to differentiate towards granulocytes . ^^^ Accumulation of C / EBP epsilon mRNA occurred as early as 1 hour after exposure of NB 4 cells to 9 cis RA ( 5 10 10 ( 7 ) mol / L ) ; and at 48 hours , levels were increased by 5 . 1 fold . ^^^ Dose response studies showed that 10 ( 7 ) to 10 ( 6 ) mol / L 9 cis RA ( 12 hours ) resulted in peak levels of C / EBP epsilon mRNA ; but even 10 ( 10 ) mol / L 9 cis RA increased levels of these transcripts . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP epsilon : chromosomal mapping and mutational analysis of the gene in leukemia and preleukemia . ^^^ We and others have cloned a novel human gene CCAAT / enhancer binding protein epsilon ( C / EBP epsilon ) encoding a member of the C / EBP gene family . ^^^ We used a polymerase chain reaction ( PCR ) based technique to examine DNA from 93 hamster / human radiation hybrid clones in order chromosomally to map C / EBP epsilon to 14q11 . 2 ( between D14S264 and D14S275 ) which is telomeric to the T cell receptor alpha and delta genes and centromeric to several other myeloid gene products including Cathepsin G ( CTSG ) and Chymase 1 ( CMA 1 ) . ^^^ To determine whether C / EBP epsilon behaves as an altered tumor suppressor gene , samples from patients with acute myelogenous leukemia ( AML ) and myelodysplastic syndrome ( MDS ) evolving to AML were studied for loss of heterozygosity ( LOH ) using microsatellite sequences that we identified within 0 . 2 kb of the amino terminus of the human C / EBP epsilon gene . ^^^ Allelic loss of the C / EBP epsilon gene was detected in four out of 20 ( 20 % ) evolving MDS cases and in none of the 17 AML and 17 T cell leukemia cases . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| The enhancer contains two elements that bind C / EBP alpha and beta in U 937 cells , while C / EBP epsilon is also bound in extracts of HL 60 cells . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| The Hoxa 9 immortalized progenitor exhibited a promyelocytic transcriptional profile , expressing PU . 1 , AML 1 , c Myb , C / EBP alpha , and C / EBP epsilon as well as their target genes , the receptors for GM CSF , G CSF , and M CSF and the primary granule proteins myeloperoxidase and neutrophil elastase . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Representational difference analysis using myeloid cells from C / EBP epsilon deletional mice . ^^^ C / EBP epsilon is a recently cloned member of the C / EBP family of transcriptional factors . ^^^ C / EBP epsilon deficient mice developed normally but failed to generate functional neutrophils and eosinophils , and these mice died of opportunistic infections suggesting that C / EBP epsilon may play a central role in myeloid differentiation . ^^^ To identify myelomonocytic genes regulated by the C / EBP epsilon gene , we performed representational difference analysis ( RDA ) , a polymerase chain reaction ( PCR ) based subtractive hybridization using neutrophils and macrophages from wild type and C / EBP epsilon knockout mice . ^^^ Taken together , we have found several genes whose expression might be enhanced by C / EBP epsilon . ( Blood . 2000 ; 96 : 3953 3957 ) . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Differentiation of hematopoietic cells is regulated by lineage specific transcription factors , and gene targeting studies previously revealed the critical roles of CCAAT / enhancer binding protein ( C / EBP ) alpha and C / EBP epsilon , respectively , in the early and mid late stages of granulocyte differentiation . ^^^ The expression of C / EBP epsilon in 32Dcl3 cells and FDCP 1 cells expressing mutant G CSF receptors was examined and it was found that G CSF up regulates C / EBP epsilon . ^^^ Dominant negative signal transducers and activators of transcription 3 blocked G CSF induced granulocytic differentiation in 32D cells but did not block induction of C / EBP epsilon , indicating that these proteins work in different pathways . ^^^ It was also found that overexpression of C / EBP epsilon greatly facilitated granulocytic differentiation by G CSF and , surprisingly , that expression of C / EBP epsilon alone was sufficient to make cells differentiate into morphologically and functionally mature granulocytes . ^^^ In 32Dcl3 cells overexpressing c myc that do not differentiate by means of G CSF , induction of C / EBP epsilon is completely abrogated . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP epsilon of Japanese flounder was 1861 bp in length ( ORF of 822 bp ) encoding for 274 amino acids , with a calculated molecular weight of 30 kDa . ^^^ C / EBP epsilon was detected by Northern blot from total RNA of head and posterior kidney , heart and spleen . ^^^ However , RT PCR also detected C / EBP epsilon in brain , spleen and peritoneal cavity fluid and peripheral blood leucocyte cDNA . ^^^ However , Japanese flounder C / EBP epsilon sequence data were not found to cluster with the three reported mammalian C / EBP epsilon sequences currently available . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Macrophage functional maturation and cytokine production are impaired in C / EBP epsilon deficient mice . ^^^ Unlike the other members of the family , C / EBP epsilon expression is restricted to granulocytes , macrophages , and lymphocytes . ^^^ C / EBP epsilon is highly conserved between human and rodents and is essential for terminal granulopoiesis in both species . ^^^ To study the role that C / EBP epsilon plays in macrophages , wild type and C / EBP epsilon deficient ( / ) murine macrophages obtained from thioglycollate elicited peritoneal lavages and differentiated bone marrow cells were compared . ^^^ Although macrophage development occurred in both types of mice , the C / EBP epsilon / cells had a lower expression of macrophage markers and a morphologic and ultrastructural appearance of immaturity . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Introduction of an inducible C / EBP alpha gene into the line revealed that conditional expression of C / EBP alpha induced the C / EBP family members C / EBP beta and C / EBP epsilon and subsequent granulocyte differentiation . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| We previously localized transcriptional activation and inhibitory regions in one family member , C / EBP epsilon . ^^^ Here we describe the further characterization of a C / EBP epsilon inhibitory domain termed regulatory domain 1 . ^^^ SUMO 1 attachment decreased the inhibitory effect of the C / EBP epsilon regulatory domain , suggesting that sumoylation may play an important role in modulating C / EBP epsilon activity as well as that of the other C / EBP family members . . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Regulation of neutrophil and eosinophil secondary granule gene expression by transcription factors C / EBP epsilon and PU . 1 . ^^^ In the bone marrow of C / EBP epsilon ( / ) mice , expression of neutrophil secondary and tertiary granule mRNAs is absent for lactoferrin ( LF ) , neutrophil gelatinase ( NG ) , murine cathelin like protein ( MCLP ) , and the cathelin B 9 ; it is severely reduced for neutrophil collagenase ( NC ) and neutrophil gelatinase associated lipocalin ( NGAL ) . ^^^ Stable inducible expression of C / EBP epsilon and C / EBP alpha in the murine fibroblast cell line NIH 3T3 activated expression of mRNAs for B 9 , MCLP , NC , and NGAL but not for LF . ^^^ In transient transfections of C / EBP epsilon and C / EBP alpha , B 9 was strongly induced with weaker induction of the other genes . ^^^ The expression of MBP was inefficiently induced by C / EBP epsilon alone and weakly induced with C / EBP epsilon and GATA 1 , but the addition of PU . 1 resulted in a striking cooperative induction of MBP in NIH 3T3 cells . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation ( ChIP ) studies indicate a role for CCAAT enhancer binding proteins alpha and epsilon ( C / EBP alpha and C / EBP epsilon ) and CDP / cut in myeloid maturation induced lactoferrin gene expression . ^^^ In vitro models of granulopoiesis involving the inducible expression of either CCAAT enhancer binding protein alpha ( C / EBP alpha ) or C / EBP epsilon in myeloid cells have been shown to lead to the induction of a granulocytic maturation program accompanied by the expression of myeloid specific genes . ^^^ Upon induction of maturation , C / EBP epsilon binds to the LF promoter , which correlates with LF expression . ^^^ We conclude that C / EBP alpha , C / EBP epsilon , and CDP / cut all play definitive roles in regulating late gene expression during normal myeloid development . . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Antibody supershift experiments revealed that LPS induced C / EBP DNA binding activity depended on C / EBP beta and C / EBP delta but not C / EBP alpha , C / EBP epsilon or CBP / p300 . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Although RAR alpha did not directly regulate Mad 1 or p 27 ( Kip 1 ) , the RAR alpha target gene C / EBP epsilon directly regulated transcription of Mad 1 . ^^^ Induction of C / EBP epsilon activity in granulocytic cells led to rapid induction of Mad 1 protein and transcript , with direct binding of C / EBP epsilon to the Mad 1 promoter demonstrated through chromatin immunoprecipitation assay . ^^^ These data demonstrate that cell cycle arrest in response to RAR alpha specifically requires Mad 1 and p 27 ( Kip 1 ) and that Mad 1 is transcriptionally activated by CCAAT / enhancer binding protein epsilon ( C / EBP epsilon ) . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| CCAAT / enhancer binding protein ( C / EBP ) alpha , PU . 1 , granulocyte colony stimulating factor receptor , PR 3 , C / EBP epsilon and lactoferrin were sequentially expressed during neutrophil differentiation but rapidly down regulated during the early erythroid stages . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Mouse chromosomal location of the CCAAT / enhancer binding proteins C / EBP beta ( Cebpb ) , C / EBP delta ( Cebpd ) , and CRP 1 ( Cebpe ) . ^^^ There are four known members of the C / EBP family of basic region leucine zipper transcription factors : Cebpa , Cebpb , Cebpd , and Cebpe . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| Three genes were isolated that encode bZIP DNA binding proteins ( designated CRP 1 , CRP 2 , and CRP 3 ) with strong amino acid sequence similarities to the C / EBP binding domain . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| The C / EBP related proteins ( C / EBPalpha , CRP 1 , C / EBPbeta , and C / EBPdelta ) form a subfamily of bZIP ( basic region / leucine zipper ) transcription factors that display sequence homology within the bZIP domain . ^^^ A basic residue within the CRP 1 NLS that is not conserved within the C / EBP family is occupied instead by an uncharged residue in C / EBPalpha and C / EBPbeta . ^^^ |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15744 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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