| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Introduction of an inducible C / EBP alpha gene into the line revealed that conditional expression of C / EBP alpha induced the C / EBP family members C / EBP beta and C / EBP epsilon and subsequent granulocyte differentiation . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Antibody supershift experiments revealed that LPS induced C / EBP DNA binding activity depended on C / EBP beta and C / EBP delta but not C / EBP alpha , C / EBP epsilon or CBP / p300 . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Mouse chromosomal location of the CCAAT / enhancer binding proteins C / EBP beta ( Cebpb ) , C / EBP delta ( Cebpd ) , and CRP 1 ( Cebpe ) . ^^^ There are four known members of the C / EBP family of basic region leucine zipper transcription factors : Cebpa , Cebpb , Cebpd , and Cebpe . ^^^ Here , we show that Cebpb maps to mouse chromosome 2 , Cebpd to chromosome 16 , and Cebpe to chromosome 14 . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| By combining genetic studies and footprinting analyses , we demonstrate that regulated expression of the CytR controlled cdd promoter requires three CRP binding sites : a high affinity site ( CRP 1 ) and two overlapping low affinity sites ( CRP 2 and CRP 3 ) centred at positions 41 , 91 and 93 , respectively . ^^^ In the absence of CytR , cAMP CRP interacts at one set of sites ( CRP 1 and CRP 2 ) and both of these binding sites are required for full promoter activation . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| We show that the cAMP CRP activator complex recognizes two sites in tsx p 2 that are separated by 33 bp : a high affinity site ( CRP 1 ) overlaps the 35 region , and a low affinity site ( CRP 2 ) is centered around position 74 bp . ^^^ We characterized four tsx p 2 mutants exhibiting a reduced response to CytR ; three carried mutations in the CRP 2 site , and one carried a mutation in the region between CRP 1 and the 10 sequence . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| CRP 2 is identical to the protein described recently by other groups as NF IL 6 , LAP , IL 6DBP , and AGP / EBP , whereas CRP 1 and CRP 3 represent novel proteins . ^^^ Three genes were isolated that encode bZIP DNA binding proteins ( designated CRP 1 , CRP 2 , and CRP 3 ) with strong amino acid sequence similarities to the C / EBP binding domain . ^^^ CRP 2 is identical to the protein described recently by other groups as NF IL 6 , LAP , IL 6DBP , and AGP / EBP , whereas CRP 1 and CRP 3 represent novel proteins . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| One of these sites , CRP 1 , overlaps the 35 region , and is sufficient for activation ; the second site , CRP 2 , centred around 93 , is indispensable for repression . ^^^ Here we demonstrate , by means of in vivo titration , that CytR interaction with deoP 2 depends not only on CRP 2 , but also on CRP 1 and the length and possibly the sequence separating these two sites . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| As determined in DNase 1 protection experiments , two binding sites for the complex of cyclic AMP and the receptor protein were identified about 60 base pairs ( CRP 1 ) and 110 base pairs ( CRP 2 ) upstream from the transcription initiation site of the colicin E 1 gene . ^^^ CRP 1 had a higher affinity for the complex than that of CRP 2 . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| We have identified and characterized two evolutionarily conserved members of the CRP family , CRP 1 and CRP 2 , in chicken and quail . ^^^ Expression of the genes encoding both CRP 1 and CRP 2 is differentially regulated in normal versus transformed cells , raising the possibility that members of the CRP family may function in control of cell growth and differentiation . . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| In fact , two slightly different forms ( CRP 1 and CRP 2 ) are expressed in the prostate ; one of them ( CRP 1 ) is also expressed in the exorbital lacrymal gland . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| In the CRP 2 CytR complex all protein DNA phosphate contacts at CRP 1 and CRP 2 are retained , and in addition two new minor groove contacts , ascribed to phosphate CytR interactions , are observed at 60 between the CRP sites . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Two complete 6 kb genes , Crp 1 and Crp 2 , have been cloned and characterized . ^^^ The 5 ' flanking regions of Crp 1 and Crp 2 are highly homologous and contain a GATAAA sequence 29 nt upstream from the transcription start point . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| The results indicate that CytR binds specifically to multiple sites at deoP 2 , including both the well recognized CytR site flanked by CRP 1 and CRP 2 and also sites coincident with CRP 1 and CRP 2 . ^^^ Based on these findings we propose that CytR functions as a differential modulator of CRP 1 versus CRP 2 mediated activation . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| These tetrapeptides , resembling the aminoacid sequence of tuftsin ( CRP 1 , H gThr ( R , S ) mLys Pro Leu OH , ITF 1192 ; CRP 2 , H gGly ( R , S ) mLys Pro Arg OH , ITF 1127 ; CRP 3 , H gThr ( R , S ) mLys Pro Gln OH . ^^^ ITF 1193 ) , were able to induce NO synthesis by peritoneal macrophages in a dose dependent manner ; the most stimulating dose was 1000 ng ml 1 for CRP 2 and 100 ng ml 1 for CRP 1 and CRP 3 . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Proteins of the cysteine rich protein ( CRP ) family ( CRP 1 , CRP 2 , and CRP 3 ) are implicated in diverse processes linked to cellular differentiation and growth control . ^^^ The global fold of quail CRP 2 LIM2 is very similar to that of the carboxyl terminal LIM domain of the related but functionally distinct CRP family member CRP 1 , analyzed recently . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Additional AR binding sites , ARBSu / crp1 and ARBSu / crp2 , occur 5 ' upstream of the transcription start point and are located at an identical position ( 142 / 120 ) in crp 1 and crp 2 . ^^^ A large fragment derived from intron 1 of the crp 1 and crp 2 gene can also provide the androgen dependent transcription of chimeric constructs in T 47D cells . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| In this study , we have addressed the biological significance of the CRP multigene family by comparing the subcellular distributions , biochemical properties , and expression patterns of CRP 1 , CRP 2 , and CRP3 / MLP . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Members of the cysteine and glycine rich protein ( CRP ) family ( CRP 1 , CRP 2 , and CRP 3 ) contain two zinc binding LIM domains , LIM 1 and LIM 2 , and are implicated in diverse cellular processes linked to differentiation , growth control and pathogenesis . ^^^ The structural analysis revealed that the global fold of LIM 1 closely resembles the recently determined solution structures of the carboxyl terminal LIM 2 domains of quail CRP 2 and chicken CRP 1 , and that LIM 1 and LIM 2 are independently folded structural and presumably functional domains of CRP proteins . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| DNase 1 footprinting experiments localized two CRP binding sites in this region : CRP 1 , which is positioned upstream of P 1 P3 , and CRP 2 , which is located within the promoters . ^^^ Site directed mutagenesis of each site provided evidence that CRP 1 is necessary for the effects of cyclic AMP CRP on dad expression in vivo and in vitro , and that CRP 2 probably plays little or no role in this process . . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Members of the cysteine and glycine rich protein family ( CRP 1 , CRP 2 , and CRP 3 ) contain two zinc binding LIM domains , LIM 1 ( amino terminal ) and LIM 2 ( carboxyl terminal ) , and are implicated in diverse cellular processes linked to differentiation , growth control , and pathogenesis . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| TCRP encodes a putative protein with two LIM domains linked to a short glycine rich region that displays 86 % , 76 % , 67 % identity with chicken CRP 2 , CRP 1 and MLP / CRP3 proteins , respectively . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Recently , we showed that CytR binds specifically to multiple sites in the E . coli deoP promoter , thereby providing competition for CRP binding to CRP operator site 1 ( CRP 1 ) and CRP 2 as well as cooperativity . ^^^ Cooperativity with CRP at CRP 1 is nearly eliminated , but the effect on CytR CRP 2 cooperativity is negligible . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Protein interaction assays demonstrate that MLP and betaI spectrin associate with one another in vivo as well as when tested under several in vitro binding conditions . betaI spectrin binds specifically to MLP but not to the MLP related proteins CRP 1 and CRP 2 or to other LIM domain containing proteins . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Sequences similar to the sigma ( 70 ) promoters P 1 , P 4 and P 5 , to the sigma ( E ) promoter P 3 and to boxes DnaA 1 , DnaA 2 , cAMP receptor protein ( CRP ) boxes CRP 1 , CRP 2 and box CytR present in Escherichia coli K 12 , were identified in sequences of closely related bacteria such as : E . coli , Shigella flexneri , Salmonella enterica serovar Typhimurium , Citrobacter freundii , Enterobacter cloacae and Klebsiella pneumoniae . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| After introduction of mutations affecting binding sites for CRP ( CRP 1 and CRP 2 ) , the negative effect of the CytR protein on promoter transcription was fully abolished . ^^^ The CRP 1 binding site was shown to play the main role in the activation of the promoter by the cAMP CRP complex , whereas the CRP 2 site participates in the formation of the repressor complex . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| The LIM domain proteins cysteine rich protein 1 ( CRP 1 ) and CRP 2 are expressed in sciatic nerve and induced by forskolin in cultured Schwann cells , but only CRP 2 requires pou3f1 for normal expression . pou3f1 appears to require the claw paw gene product for activation of at least some of its downstream effector genes . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Cysteine rich LIM only proteins CRP 1 and CRP 2 are potent smooth muscle differentiation cofactors . ^^^ Cysteine rich LIM only proteins , CRP 1 and CRP 2 , expressed during cardiovascular development act as bridging molecules that associate with serum response factor and GATA proteins . ^^^ Together with SRF and GATA proteins , CRP 1 and CRP 2 converted pluripotent 10T1 / 2 fibroblasts into smooth muscle cells , while muscle LIM protein CRP 3 inhibited the conversion . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Serial CRP measurements were done at admission ( CRP 1 ) , within 24 hours ( CRP 2 ) , and within 48 hours ( CRP 3 ) after symptom onset . ^^^ RESULTS : The CRP concentration increased significantly during the first 48 hours after symptom onset ( CRP 1 , 0 . 86 mg / dL [ 95 % CI , 0 . 69 to 1 . 02 ] ; CRP 2 , 1 . 22 mg / dL [ 95 % CI , 0 . 88 to 1 . 55 ] ; CRP 3 , 1 . 75 mg / dL [ 95 % CI , 1 . 25 to 2 . 25 ] ; P=0 . 003 ) . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Consistent with this proposal , we showed that CRP binds two sites , CRP 1 and CRP 2 . ^^^ Induction of acs expression absolutely required CRP 1 , while optimal expression required both CRP 1 and CRP 2 . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Thus far , the latter function has been inferred solely from the in vitro interaction of CRP 1 , CRP 2 , and CRP 3 with alpha actinin and zyxin . ^^^ |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| Blood sampling for CRP measurements were taken before the treatment ( CRP 0 ) , and during the first ( CRP 1 ) and second ( CRP 2 ) day of empiric therapy . ^^^ In the first group of 14 patients who received inappropriate treatment serum CRP concentations ( mg / L ; mean and + / SD ) were : CRP 0 = 107 . 5 + / 65 . 6 ; CRP 1 = 155 . 3 + / 75 . 7 ; CRP 2 = 209 . 1 + / 67 . 0 , while in 33 repeated samples of the 33 patients in the second group who received adequate treatment the following results were recorded : CRP 0 = 124 . 0 + / 78 . 1 ; CRP 1 = 133 . 8 + / 63 . 5 ; CRP 2 = 94 . 6 + / 46 . 4 . ^^^ |
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| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15744 and P17676 |
Pubmed |
SVM Score :0.0 |
| NA |
|