Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NF kappa B is activated following degradation of 1 kappa B alpha and 1 kappa B beta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The IKK complex in the absence of IKK 1 is capable of phosphorylating IkappaBalpha and IkappaBbeta in vitro . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
RSV CM induced degradation of IkappaBalpha within 5 min but did not affect IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We report here that NF kappa B and 1 kappa B alpha ( but not 1 kappa B beta ) are also localized in the mitochondria . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Three of these inhibitory molecules have been described : IkappaBalpha , IkappaBbeta and IkappaBepsilon . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
It was associated with sustained levels of IkappaBbeta , but not IkappaBalpha . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IkappaBalpha but not IkappaBbeta was degraded within 10 min before resynthesis by 2 h . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Here , we purified and characterized 1 kappa B beta , a chromatographically distinct second form of 1 kappa B . 1 kappa B beta has a size of 43 kDa and , as 1 kappa B alpha , an acidic isoelectric point between 4 . 8 and 5 . 0 . ^^^ In contrast to 1 kappa B alpha , 1 kappa B beta lost its inhibiting activity upon a treatment with phosphatase . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In addition , the 1 kappa B beta proteins chicken p 40 and human MAD 3 , proteins that are related to the p 105 C terminus , strongly activated transcription in chicken cells and yeast when fused to GAL 4 DNA binding sequences . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Additionally , 1 kappa B beta , but not 1 kappa B alpha , also prevented the binding of Rel to the kappa B site . 1 kappa B beta and pp 40 are related proteins because ( 1 ) they share a number of common tryptic peptides , ( 2 ) their inhibitory effect on DNA binding can be abolished by preincubation with pp 40 specific antiserum , and ( 3 ) labeled 1 kappa B beta can be immunoprecipitated with pp 40 antibodies . pp 40 is part of the Rel complex present in the cytoplasm and nuclear extracts of WEHI 231 cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Transcription factors belonging to the NF kappa B family are controlled by inhibitory 1 kappa B proteins , mainly 1 kappa B alpha and 1 kappa B beta . ^^^ In contrast to hematopoietic cells , 1 kappa B alpha / embryonic fibroblasts show minimal constitutive NF kappa B , as well as normal signal dependent NF kappa B activation that is concomitant with 1 kappa B beta degradation . ^^^ Our results indicate that 1 kappa b beta , but not 1 kappa B alpha , is required for the signal dependent activation of NF kappa B in fibroblasts . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
One class of inducers causes rapid but transient activation of NF kappa B by primarily affecting 1 kappa B alpha complexes , whereas another class of inducers causes persistent activation of NF kapa B by affecting both 1 kappa B alpha and 1 kappa B beta complexes . ^^^ Therefore , the overall activation of NF kappa B consists of two overlapping phases , a transient phase mediated through 1 kappa B alpha and a persistent phase mediated through 1 kappa B beta . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
This mechanism of degradation may also apply to 1 kappa B beta , an inhibitor related to and functionally overlapping with 1 kappa B alpha , as well as to cactus , an 1 kappa B homolog of Drosophila . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
While 1 kappa B alpha , 1 kappa B beta , and p 100 potently inhibit Tat mediated transactivation , p 105 fails to inhibit , but even moderately synergizes , the action of Tat . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In the present study , we demonstrate that Tax induces the degradation Of IkappaBbeta , another NF kappaB / Rel cytoplasmic inhibitor that differs from IkappaBalpha in signal responses . ^^^ Unlike that observed with IkappaBalpha , the degradation Of IkappaBbeta is not associated with its rapid resynthesis , apparently because of the failure of Tax to stimulate IkappaBbeta gene transcription . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In resting T lymphocytes , the transcription factor NF kappaB is sequestered in the cytoplasm via interactions with members of the 1 kappa B family of inhibitors , including IkappaBalpha and IkappaBbeta . ^^^ In this report , we provide evidence that in addition to acting on IkappaBalpha , Tax stimulates the turnover Of IkappaBbeta via a related targeting mechanism . ^^^ Like IkappaBalpha , Tax mediated breakdown of IkappaBbeta in transfected T lymphocytes is blocked either by cell permeable proteasome inhibitors or by mutation Of IkappaBbeta at two serine residues present within its N terminal region . ^^^ Despite the dual specificity of HTLV 1 Tax for IkappaBalpha and IkappaBbeta at the protein level , Tax selectively stimulates NF kappaB directed transcription of the IkappaBalpha gene . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Cultured fibroblasts derived from IkappaBalpha deficient embryos exhibit levels of NF kappaB 1 , NF kappaB 2 , RelA , c Rel , and IkappaBbeta similar to those of wild type fibroblasts . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Analysis of the effect of elevated cAMP on the degradation of 1 kappa B alpha and 1 kappa B beta did not reveal an essential change in degradation kinetics of these inhibitor proteins . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Elevated levels of NF kappa B were partially achieved through a sustained decrease in the steady state amount of the NF kappa B / Rel specific inhibitory protein , 1 kappa B alpha , and a transient decrease in 1 kappa B beta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Stimulation with inducers that cause persistent activation of NF kappa B results in the degradation of the NF kappa B inhibitors , 1 kappa B alpha and 1 kappa B beta . ^^^ It appears therefore that during prolonged stimulation , 1 kappa B beta functions as a chaperone for NF kappa B by protecting it from 1 kappa B alpha and allowing it to be transported to the nucleus . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Interestingly , activation of these NF kappaB / Rel complexes by the CD 28 signal is associated with the rapid degradation of both IkappaBalpha and IkappaBbeta , two major cytoplasmic inhibitors of NF kappaB / Rel . ^^^ Although IkappaBalpha degradation can be induced by phorbol ester alone , degradation of IkappaBbeta is largely dependent on the CD 28 costimulatory signal . ^^^ Together , these results suggest that the CD 28 costimulatory signal augments activation of NF kappaB / Rel by promoting degradation of IkappaBbeta as well as enhancing degradation of IkappaBalpha and that induction of NF kappaB / Rel serves as an essential step in the signal mediated activation of the CD28RE enhancer . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In most cell types other than mature B lymphocytes and macrophages , the transcription factor NF kappaB remains in an inactive form in the cytosol by being bound to the inhibitory proteins IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Signal initiated activation of the transcription factor NF kappaB is mediated through proteolysis of its cytoplasmic inhibitory proteins IkappaBalpha and IkappaBbeta . ^^^ While most NF kappaB inducers trigger the degradation of IkappaBalpha , only certain stimuli are able to induce the degradation of IkappaBbeta . ^^^ The degradation of IkappaBalpha is targeted by its site specific phosphorylations , although the mechanism underlying the degradation of IkappaBbeta remains elusive . ^^^ Mutational analyses have revealed that the inducible degradation of IkappaBbeta induced by calyculin A , and TNF alpha requires two N terminal serines ( serines 19 and 23 ) that are homologous to the inducible phosphorylation sites present in IkappaBalpha . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
HepG 2 cells express the IkappaB members IkappaBalpha , IkappaBbeta , and IkappaBgamma . ^^^ NF kappaB 1 translocation depends on simultaneous proteolysis of both IkappaBalpha and IkappaBbeta isoforms ; IkappaBgamma is inert to TNFalpha treatment . ^^^ NF kappaB 1 translocation depends on simultaneous proteolysis of both IkappaBalpha and IkappaBbeta isoforms ; IkappaBgamma is inert to TNFalpha treatment . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In mammalian cells , two major forms of IkappaB proteins , IkappaBalpha and IkappaBbeta , have been identified . ^^^ Upon treatment with a large variety of inducers , IkappaBalpha and IkappaBbeta are proteolytically degraded , resulting in NF kappaB translocation into the nucleus . ^^^ Surprisingly , our data suggest that unlike IkappaBalpha , IkappaBbeta is constitutively phosphorylated on one or two of the critical NH 2 terminal serine residues . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
SMC express two inhibitor proteins IkappaBbeta and IkappaBalpha . ^^^ Interleukin 1beta stimulation caused transient loss of IkappaBalpha and a sustained decrease of IkappaBbeta that correlated with increased and persistent levels of p65 / p50 protein and binding activity in the nucleus . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
CsA does not interfere with the synthesis of Rel proteins , but prevents the inducible degradation of cytosolic NF kappa B inhibitors 1 kappa B alpha and 1 kappa B beta upon T cell activation . ^^^ These results indicate that CsA interferes with a specific event in the signal induced degradation of 1 kappa B alpha and 1 kappa B beta , but does not affect the processing of NF kappa B1 / p105 to p50 . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Listeria monocytogenes infection of P388D1 macrophages results in a biphasic NF kappaB ( RelA / p50 ) activation induced by lipoteichoic acid and bacterial phospholipases and mediated by IkappaBalpha and IkappaBbeta degradation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Activation of transcription factor NF kappaB involves the signal dependent degradation of basally phosphorylated inhibitors such as IkappaBalpha and IkappaBbeta . ^^^ However , recent studies have identified a hypophosphorylated pool of IkappaBbeta that shields nuclear NF kappaB from inhibition by newly synthesized IkappaBalpha . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The levels of IkappaBalpha and IkappaBbeta were analyzed during this period in an attempt to correlate NF kappaB activity with IkappaB resynthesis . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Analysis of the kappaB inhibitory ( 1 ) proteins showed an important role for IkappaBalpha in the process of NF kappaB activation , whereas the contribution of IkappaBbeta was less evident . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The NF kappa B family members p 50 , p 65 , p 52 , c Rel , and RelB as well as the inhibitor proteins 1 kappa B alpha , 1 kappa B beta , and p 105 were present in uninjured arteries as determined by immunoblotting . ^^^ Furthermore , immediately after injury , the levels of 1 kappa B alpha , 1 kappa B beta , and p 105 were dramatically reduced . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The ligase recognition signal is functionally conserved between 1 kappa B alpha and 1 kappa B beta , and does not involve the nearby ubiquitination site . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Proteasome dependent inhibitory kappa B alpha ( 1 kappa B alpha ) and 1 kappa B beta degradation occurred downstream of CD 40 ligation and preceded CD 40 mediated NF kappa B nuclear translocation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The biological activity of the transcription factor NF kappaB is mainly controlled by the IkappaB proteins IkappaBalpha and IkappaBbeta , which restrict NF kappaB in the cytoplasm and enter the nucleus where they terminate NF kappaB dependent transcription . ^^^ In addition , IkappaBepsilon appears to function predominantly in the cytoplasm to sequester p 65 homodimers , in contrast with the other two members of the family , IkappaBalpha and IkappaBbeta , which also function in the nucleus to terminate NF kappaB dependent transcriptional activation . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Three subfamilies of 1 kappa B inhibitors were also defined : ( 1 ) NF kappa B 1 and NF kappa B 2 ; ( 2 ) close to subfamily 1 , the short 1 kappa B proteins 1 kappa B alpha , 1 kappa B beta and Bcl 3 ; ( 3 ) Relish that diverged earlier than other 1 kappa B inhibitors . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Sesquiterpene lactones specifically inhibit activation of NF kappa B by preventing the degradation of 1 kappa B alpha and 1 kappa B beta . ^^^ Treatment of cells with SLs prevented the induced degradation of 1 kappa B alpha and 1 kappa B beta by all these stimuli , suggesting that they interfere with a rather common step in the activation of NF kappa B . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Regulation of NF kappa B activity by 1 kappa B alpha and 1 kappa B beta stability . ^^^ We therefore compared the turnover of 1 kappa B alpha and 1 kappa B beta in mature B cells and HeLa cells . ^^^ Both proteins display a high turnover in B cells although 1 kappa B beta is considerably more stable than 1 kappa B alpha . ^^^ TNF alpha signaling leads to a rapid depletion of cellular 1 kappa B beta pools . 1 kappa B alpha is efficiently resynthesized whereas 1 kappa B beta levels stay low for a prolonged time . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In mammalian cells , 1 kappa B alpha and 1 kappa B beta proteins have been purified and shown to be the inhibitors of NF kappa B through their association with the p 65 or c Rel subunits . ^^^ Upon treatment with a large variety of inducers , 1 kappa B alpha , 1 kappa B beta are proteolytically degraded , resulting in NF kappa B translocation into the nucleus . ^^^ Here we show that in E29 . 1 T cell hybridoma 1 kappa B alpha and 1 kappa B beta are equally associated with p 65 and that 1 kappa B beta is degraded in response to TNF alpha in contrast to what has been originally published . ^^^ Our data also suggest that , unlike 1 kappa B alpha , 1 kappa B beta is constitutively phosphorylated and resynthesized as a hypophosphorylated form . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Overexpression of TAK 1 together with its activator protein , TAK 1 binding protein 1 ( TAB 1 ) , induced the nuclear translocation of NF kappa B p50 / p65 heterodimer accompanied by the degradation of 1 kappa B alpha and 1 kappa B beta , and the expression of kappa B dependent reporter gene . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
CpG DNA rescue from anti IgM induced WEHI 231 B lymphoma apoptosis via modulation of 1 kappa B alpha and 1 kappa B beta and sustained activation of nuclear factor kappa B / c Rel . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
EGF stimulation enhances the degradation of IkappaBalpha , but not IkappaBbeta nor an N terminal deletion mutant of IkappaBalpha . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
A 549 cells expressed the IkappaB inhibitory subunits IkappaBalpha , IkappaBbeta , and p 105 ; however , following either stimulus , only the IkappaBalpha and IkappaBbeta steady state levels declined in parallel with the increase in RelA DNA binding activity . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
CsA also inhibits both the prolonged , high rate of IkappaBalpha degradation and the lower level of IkappaBbeta turnover during the second phase of the activation response . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
During persistent activation of NF kappaB at later times in infection , syntheses of inhibitors IkappaBalpha as well as IkappaBbeta were restored . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
DNA PK also phosphorylated the NF kappa B inhibitory proteins IkB alpha and IkB beta in vitro , and deletion analysis demonstrated that DNA PK phosphorylates 2 distinct regions of IkB beta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Here we present evidence that beginning at around 6 h postinfection , herpes simplex virus ( HSV ) induces a persistent translocation of NF kappa B into the nucleus of C 33 cells , coincident with loss of both 1 kappa B alpha and 1 kappa B beta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In addition , overexpression of the dominant negative mutants of NF kappaB inhibitor molecules , IkappaBalpha and IkappaBbeta , abolished the Tax induced activation of IL 8 gene . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Functional redundancy of the nuclear factor kappa B inhibitors 1 kappa B alpha and 1 kappa B beta . ^^^ In an effort to address the functional redundancy of two closely related IkappaB molecules , IkappaBalpha and IkappaBbeta , we generated knock in mice by replacing the IkappaBalpha gene with the IkappaBbeta gene . ^^^ The knock in mice do not express IkappaBalpha , but express a T 7 tagged IkappaBbeta under the promoter and regulatory sequence of ikba . ^^^ These results indicate that IkappaBalpha and IkappaBbeta share significant similarities in their biochemical activity , and that they acquired their different functions from divergent expression patterns during evolution . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Lipopolysaccharide induced NF kappaB activation in human endothelial cells involves degradation of IkappaBalpha but not IkappaBbeta . ^^^ LPS induced IkappaBalpha degradation at 30 60 min after treatment , but did not induce IkappaBbeta degradation up to 120 min . ^^^ In contrast , TNF alpha rapidly induced IkappaBalpha degradation within 5 min and IkappaBbeta degradation within 15 min . ^^^ We conclude that in HUVEC , LPS induces NF kappaB dependent genes through degradation of IkappaBalpha , not IkappaBbeta , and propose that this degradation is induced in part by HMA sensitive kinase ( s ) upstream of IkappaBalpha . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
METHODS : The expression of NF kappa B subunits p 65 and p 50 and the inhibitory subunits 1 kappa B alpha and 1 kappa B beta in human and rat myocardial samples was measured by immunoblotting , using antibodies , specific to each subunit . ^^^ Depletion of the inhibitory factors 1 kappa B alpha and 1 kappa B beta was assessed by immunoblotting . ^^^ RESULTS : p 65 , p 50 , 1 kappa B alpha and 1 kappa B beta are expressed at all stages of development analysed . ^^^ In rat myocardium , p 65 , p 50 , 1 kappa B alpha and 1 kappa B beta showed a gradual decline during development , with a particularly pronounced decrease between the ten day post natal and adult samples . ^^^ CONCLUSIONS : The principal NF kappa B subunits p 65 , p 50 , 1 kappa B alpha and 1 kappa B beta are present throughout development , suggesting that this transcription complex may participate in myocardial gene regulation throughout development and in the adult . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Phosphorylation of the IkappaB cytoplasmic inhibitors , IkappaBalpha , IkappaBbeta , and IkappaBepsilon , by these kinases triggers proteolytic degradation and the release of NF kappaB / Rel proteins into the nucleus . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
This biphasic kinetics could result from observed transient degradation of the inhibitory protein IkappaBalpha and slower but sustained degradation of IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Dexamethasone injection induced the expression of IkappaBalpha and IkappaBbeta in thymocytes and down regulated NF kappaB DNA binding activated by intrathymic signals . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We recently purified a large multiprotein complex , the IkappaB kinase ( IKK ) signalsome , which contains two regulated IkappaB kinases , IKK 1 and IKK 2 , that can each phosphorylate IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In the inflamed lungs , inhibition of NF kappaB activation by SLPI was associated with elevated levels of lung IkappaBbeta ( but not IkappaBalpha ) protein in the absence of elevated mRNA for IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Within minutes , LPS through CD 14 induced the activation of NF kappaB , degradation of IkappaBalpha ( inhibitory subunit of NF kappaB ) and IkappaBbeta , and nuclear translocation of p 65 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We have examined the consequences of overexpression of the IkappaBalpha and IkappaBbeta inhibitory proteins on the regulation of NF kappaB dependent beta interferon ( IFN beta ) gene transcription in human cells after Sendai virus infection . ^^^ IkappaBalpha exhibited a strong inhibitory effect on virus induced IFN beta expression , whereas IkappaBbeta exerted an inhibitory effect only at a high concentration . ^^^ Inhibition of IFN beta expression directly correlated with a reduction in the binding of NF kappaB ( p 50 RelA ) complex to PRDII after Sendai virus infection in IkappaBalpha expressing cells , whereas IFN beta expression and NF kappaB binding were only slightly reduced in IkappaBbeta expressing cells . ^^^ These experiments demonstrate a major role for IkappaBalpha in the regulation of NF kappaB induced IFN beta gene activation and a minor role for IkappaBbeta in the activation process . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Persistent HIV infection of these cells results in increased levels of NF kappaB in the nucleus secondary to increased IkappaBalpha , IkappaBbeta , and IkappaBepsilon degradation , a mechanism postulated to regulate viral persistence . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
DNA complexes from HIV 1 infected cell extracts did not completely dissociate the complexes , suggesting that IkappaBbeta may protect NF kappaB complexes from IkappaBalpha mediated dissociation . ^^^ DNA binding complex that was sensitive to IkappaBalpha mediated dissociation , thus demonstrating the protective role of IkappaBbeta . ^^^ In addition , co transfection studies with an NF kappaB dependent reporter construct demonstrated that IkappaBbeta co expression partially alleviated inhibition of NF kappaB mediated gene expression by IkappaBalpha , implying that IkappaBbeta can maintain transcriptionally active NF kappaB . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IkappaBalpha levels were not affected by taxol but were reduced by etoposide treatment , while IkappaBbeta levels did not change with drug treatment . ^^^ E 2 did not alter the levels of IkappaBalpha or IkappaBbeta . ^^^ Interestingly , levels of IkappaBalpha and IkappaBbeta declined in etoposide treated ECV cells on ECM concomitant with the elevation of NFkappaB , suggesting that in these cells degradation of IkappaB may be responsible for NFkappaB activation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Role of IkappaBalpha and IkappaBbeta in the biphasic nuclear translocation of NF kappaB in TNFalpha stimulated astrocytes and in neuroblastoma cells . ^^^ In infectious diseases of the central nervous system astrocytes respond to inflammatory cytokines like tumor necrosis factor alpha ( TNFalpha ) by activation of the transcription factor NF kappaB , mediated by the proteolysis of its inhibitors IkappaBalpha and IkappaBbeta . ^^^ Degradation of both IkappaBalpha and IkappaBbeta contributed to the late phase of induction . ^^^ However , the second peak occurred also in the absence of IkappaBbeta proteolysis , demonstrating the importance of IkappaBalpha in the formation of the biphasic nuclear translocation of NF kappaB . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Treatment of cultured peritoneal macrophages with IFN gamma resulted in tyrosine phosphorylation of IkappaBalpha and IkappaBbeta , NF kappaB activation , and expression of inducible NO synthase ( iNOS ) . ^^^ Treatment with POV of macrophages stimulated with IFN gamma or LPS potentiated the degradation of IkappaBalpha and IkappaBbeta , the activation of NF kappaB , and the expression of iNOS . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
PNU 156804 inhibition of NF kappa B activation is due to the inhibition of the degradation of 1 kappa B alpha and 1 kappa B beta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
To understand the mechanisms of constitutive NF kappaB activation , we have analyzed the function of IkappaBalpha and IkappaBbeta in breast cancer cells . ^^^ In most cases , constitutive NF kappaB DNA binding correlated with reduced levels of either IkappaBalpha or IkappaBbeta isoforms . ^^^ Overexpression of IkappaBalpha but not IkappaBbeta 1 resulted in reduced constitutive DNA binding of NF kappaB in MDA MB 231 cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
TPCK inhibited constitutive NF kappaB activation in T . parva transformed T cells , with phosphorylation of IkappaBalpha and IkappaBbeta being inhibited with different kinetics . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
RESULTS : NF kappaB activation triggered by PMA was not associated with the disappearance of immunoreactive IkappaBalpha , IkappaBbeta , IkappaBgamma , or IkappaBepsilon or with the dissociation of intact IkappaB from RelA . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Taken together , DZA promotes the proteolytic degradation of IkappaBalpha , but not IkappaBbeta , resulting in an increase of DNA binding activity of NF kappaB in the nucleus in the absence of its transcriptional activity in RAW 264 . 7 cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We show that while this `` classical ' ' mode of NF kappa B activation is a uniform feature of IgM+ B cell lines , all IgG+ B cells analyzed contain nuclear NF kappa B yet have stable 1 kappa B alpha , 1 kappa B beta , and 1 kappa B epsilon . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NF kappaB heterodimers reside in the cytoplasm of cells bound to inhibitory proteins , the two commonest of which are IkappaBalpha and IkappaBbeta , which prevent NF kappaB from entering the nucleus . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Furthermore , we analyzed the protein levels of IkappaBalpha and found that , in me ( 5 ) , this inhibitor was significantly reduced , while the level of another member of the IkappaB family , IkappaBbeta , was not . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Tax expression triggers persistent phosphorylation and degradation of the NF kappaB inhibitory proteins IkappaBalpha and IkappaBbeta , resulting in constitutive nuclear expression of NF kappaB . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Of six flavonoids tested , myricetin was found to strongly inhibit IKK kinase activity , and prevent the degradation of IkappaBalpha and IkappaBbeta in activated endothelial cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The time dependent fall of the mRNA levels of NOS 2 and COX 2 paralleled the inhibition of NF kappaB , determined by electrophoretic mobility shift assays , and the increase of the IkappaBalpha and IkappaBbeta inhibitory proteins . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
This transcription factor is regulated by a family of structurally related inhibitors including IkappaBalpha , IkappaBbeta , and IkappaBepsilon , which trap NF kappaB in the cytoplasm . ^^^ LPS activated IKK complexes phosphorylate all 3 inhibitors of NF kappaB : IkappaBalpha , IkappaBbeta , and IkappaBepsilon . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We show that ceramide treatment results in reduced levels of phosphorylated IkappaBalpha and degradation of both IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Common pathway for the ubiquitination of IkappaBalpha , IkappaBbeta , and IkappaBepsilon mediated by the F box protein FWD 1 . ^^^ However , in contrast to IkappaBalpha and IkappaBbeta , the recognition site in IkappaBepsilon for FWD 1 is not restricted to the DSGPsiXS motif ; FWD 1 also interacts with other sites in the NH ( 2 ) terminal region of IkappaBepsilon . ^^^ Substitution of the critical serine residues in the NH ( 2 ) terminal regions of IkappaBalpha , IkappaBbeta , and IkappaBepsilon with alanines also markedly reduced the extent of FWD 1 mediated ubiquitination of these proteins and increased their stability . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Therefore , these results indicate that beta TrCP plays a critical role in the activation of NF kappaB by assembling the ubiquitin ligase complex for both phosphorylated IkappaBalpha and IkappaBbeta . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The release of NF kappa B factors to the nucleus requires phosphorylation and degradation of the inhibitory kappa B proteins ( 1 kappa Bs ) . 1 kappa B alpha and 1 kappa B beta phosphorylation is dependent on dual signaling by the TCR and the CD 28 accessory receptor . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The molecular mechanism mediating this effect appears to involve repression of NF kappaB activation at the level of IkappaBalpha and IkappaBbeta degradation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We noted that extracts containing Tax had extremely low levels of IkappaBbeta , but not IkappaBalpha , and contained predominantly a truncated form of the MAP3K MEKK 1 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Three major inhibitors of the NF kappaB / Rel family of transcription factors , IkappaBalpha , IkappaBbeta , and IkappaBepsilon , have been described . ^^^ The failure of observable augmentation of constitutive nuclear NF kappaB / Rel binding activity is probably due to compensatory mechanisms involving IkappaBalpha and IkappaBbeta , which are up regulated in several organs . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We found that FK 506 induces the degradation of both IkappaBalpha and IkappaBbeta and that the time courses of the FK 506 induced degradation are quite different from degradation induced by interleukin 1 ( IL 1 ) . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The levels of 1 kappa B alpha mRNA were similar after 30 , 60 , and 180 min , but the 1 kappa B beta mRNA concentration was initially low and increased over time under all conditions . 1 kappa B alpha and 1 kappa B beta protein production was not affected by the chemical protease inhibitors . ^^^ Our data show that TNF alpha production by LPS stimulated AMs from asymptomatic HIV seropositive and seronegative individuals is regulated via the phospholipase C pathway and by NF kappa B DNA binding activity without obvious changes in 1 kappa B alpha or 1 kappa B beta protein concentrations . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
TF 3 strongly inhibited both IKK 1 and IKK 2 activity and prevented the degradation of IkappaBalpha and IkappaBbeta in activated macrophage cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Here we show that in response to poly ( rI ) . poly ( rC ) ( pIC ) , PKR activates IkappaB kinase ( IKK ) , leading to the degradation of the inhibitors IkappaBalpha and IkappaBbeta and the concomitant release of NF kappaB . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
These proteins , kappaB Ras 1 and kappaB Ras 2 , interact with the PEST domains of IkappaBalpha and IkappaBbeta [ inhibitors of the transcription factor nuclear factor kappa B ( NF kappaB ) ] and decrease their rate of degradation . ^^^ In cells , kappaB Ras proteins are associated only with NF kappaB : IkappaBbeta complexes and therefore may provide an explanation for the slower rate of degradation of IkappaBbeta compared with IkappaBalpha . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Incubation of activated macrophages with 15dPGJ ( 2 ) inhibited the degradation of IkappaBalpha and IkappaBbeta and increased their levels in the nuclei . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
A CRM 1 binding export sequence was identified in the N terminal domain of IkappaBalpha but not in that of IkappaBbeta or IkappaBepsilon . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In vascular endothelial cells , 1 kappa B epsilon associates predominantly with the NF kappa B subunit Rel A and to a lesser extent with c Rel , whereas 1 kappa B alpha and 1 kappa B beta associate with Rel A only . ^^^ Following stimulation with TNF alpha , pyrrolidine dithiocarbamate ( PDTC ) , N acetylcysteine , and dexamethasone prevented 1 kappa B kinase induced 1 kappa B alpha , but not 1 kappa B beta or 1 kappa B epsilon phosphorylation and degradation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The exon / intron structure of IkappaBbeta and IkappaBalpha genes were compared and found to be very similar , with individual ankyrin repeats being maintained on corresponding exons . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We have applied these principles in order to form crystals of the Rel homology region of transcription factor NF kappaB in complex with its inhibitors IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In control C57BL / 6 mice , expression levels of p 65 , IkappaBalpha , and IkappaBbeta were 5 to 18 fold higher in the HP region , yet NF kappaB was activated in a minority of endothelial cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Moreover , FGF 2 also stimulated the transient degradation of IkappaBalpha and IkappaBbeta . ^^^ In these sulfated glycosaminoglycan deficient cells , no degradation of IkappaBalpha and IkappaBbeta was observed after FGF 2 addition . ^^^ However , in chlorate treated cells , the addition of heparin or purified HSPGs simultaneously with FGF 2 restored DNA synthesis , the sustained phosphorylation of p42 / 44 ( MAPK ) and p 90 ( RSK ) , and the degradation of IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Immunohistochemical analysis of the mouse colon revealed a high level of IkappaBbeta expression in epithelial cells relative to the rest of the tissue , whereas IkappaBalpha was found primarily in cells of the lamina propria . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We then delineated the pathway through which 2 AAF activates NF kappa B . 2 AAF treatment led to the increase of intracellular reactive oxygen species ( ROS ) which causes activation of IKK kinases , degradation of 1 kappa B beta ( but not 1 kappa B alpha ) , and increase in NF kappa B DNA binding activity . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Activation of nuclear factor kappa B ( NF kappa B ) was demonstrated by electrophoretic mobility shift assay , presence of inhibitory kappa B alpha and beta ( 1 kappa B alpha and 1 kappa B beta ) by Western blot analysis , and TNF alpha protein production by enzyme linked immunosorbent assay . ^^^ Elastase , carboxypeptidase A , and lipase induced degradation of 1 kappa B beta ( but not 1 kappa B alpha ) , activation of NF kappa B , and production of TNF alpha protein , whereas inhibition of 1 kappa B with pyrrolidine dithiocarbamate attenuated this response . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Total myocardial 1 kappa B alpha protein level is elevated 3 . 5 to 6 . 5 fold with a concomitant 50 60 % decrease in the level of 1 kappa B beta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Pretreatment ( for 1 h ) of murine macrophages or rat aortic smooth muscle cells ( activated with endotoxin ) with calpain inhibitor 1 attenuated the binding of activated NF kappaB to DNA and the degradation of IkappaBalpha , IkappaBbeta , and IkappaBvarepsilon . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Here we show that , unlike IkappaBalpha , IkappaBbeta and IkappaBepsilon appear to sequester p 65 or c Rel in the cytoplasm by inhibiting nuclear import . ^^^ Furthermore , because IkappaBbeta does not undergo nucleocytoplasmic shuttling , it can not remove nuclear proteins like IkappaBalpha does . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
HTB up to 3 mM did not inhibit the nuclear translocation of NK kappaB / Rel proteins as judged from electrophoretic mobility shift assays ; however , HTB inhibited the degradation of IkappaBbeta without significantly affecting the degradation of both IkappaBalpha and IkappaBepsilon . 4 . ^^^ In keeping with their inhibitory effect on IkappaBbeta degradation in the cell lysates , both HTB and triflusal inhibited the phosphorylation of GST IkappaBbeta elicited by TNF alpha , without affecting the phosphorylation of GST IkappaBalpha . 5 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Western blots of normal human dermal fibroblast cytoplasmic extracts showed that dimethylfumarate has minor effects on the 1 kappa B alpha , beta and epsilon proteins : their cytokine induced degradation and resynthesis is only slowed down , an effect most prominently observed for 1 kappa B beta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Surprisingly , however , neither the level of endogenous p 65 nor that of IkappaBalpha and IkappaBbeta is altered by low potassium treatment . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The effect correlates with the rapid degradation of IkappaBalpha , a sustained degradation of IkappaBbeta and increases in tumour necrosis factor alpha ( TNF alpha ) and interleukin ( IL ) 6 production , two cytokines controlled by NF kappaB . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
However , aging did not affect the protein levels of the main 1 kappa B inhibitors ( 1 kappa B alpha and 1 kappa B beta ) or 1 kappa B kinase ( IKK ) complex subunits ( IKK alpha , beta , and gamma ) involved in NF kappa B activation . ^^^ Furthermore , the expression of NF kappa B mRNAs ( p 50 , p 52 , p 65 , and c rel ) and the mRNAs of their inhibitors ( 1 kappa B alpha and 1 kappa B beta ) did not show any statistically significant age related changes . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We employed a retroviral infection approach to stably express transdominant ( TD ) mutants of IkappaBalpha , IkappaBbeta , or IkappaBepsilon and dominant negative ( dn ) versions of IkappaB kinases ( IKK ) 1 or 2 as well as a constitutively active version of IKK 2 in human endothelial cells . ^^^ TD IkappaBalpha , IkappaBbeta , and IkappaBepsilon were not degraded upon TNF alpha exposure , and each prevented NF kappaB activation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We show that although IkappaBalpha and another IkappaB member , IkappaBbeta , can enter the nucleus and repress NF kappaB DNA binding activity during the postinduction phase , only IkappaBalpha allows the efficient export of nuclear NF kappaB . ^^^ Moreover , swapping the N terminal region of IkappaBbeta for the corresponding IkappaBalpha sequence is sufficient for the IkappaB chimera protein to export NF kappaB similarly to IkappaBalpha during the postinduction state . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Compound G inhibited the activation of nuclear factor kappa B through the impairment of the targeting and degradation of 1 kappa B proteins and promoted a redistribution of 1 kappa B alpha and 1 kappa B beta in the nucleus of activated cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IKKgamma / NEMO binding to wild type , but not to a kinase deficient IKKbeta protein , facilitated the association of IkappaBalpha and IkappaBbeta with the high molecular weight IKK complex . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Two of these polypeptides , IKK alpha and IKK beta , also known as IKK 1 and IKK 2 , are the catalytic subunits of the kinase complex and phosphorylate 1 kappa B alpha and 1 kappa B beta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IkappaBbeta , but not IkappaBalpha , functions as a classical cytoplasmic inhibitor of NF kappaB dimers by masking both NF kappaB nuclear localization sequences in resting cells . ^^^ In this study , we have combined in vitro biochemical and cell based methods to elucidate differences in NF kappaB regulation exhibited by the inhibitors IkappaBbeta and IkappaBalpha . ^^^ We show that although both IkappaBalpha and IkappaBbeta bind to NF kappaB with similar global architecture and stability , significant differences exist that contribute to their unique functional roles . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Similar to other NF kappaB inducers , CpG ODN caused an early IkappaBalpha and IkappaBbeta degradation plus cleavage of the p 50 precursor and subsequent NF kappaB nuclear translocation . ^^^ As inhibitory S ODN did not directly interfere with the NF kappaB DNA binding but prevented CpG induced NF kappaB nuclear translocation of p 50 , p 65 , and c Rel and blocked p 105 , IkappaBalpha , and IkappaBbeta degradation , we concluded that their putative target must lie upstream of inhibitory kinase ( IKK ) activation . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Enhanced proteolysis of IkappaBalpha and IkappaBbeta proteins in astrocytes by Moloney murine leukemia virus ( MoMuLV ) ts 1 infection : a potential mechanism of NF kappaB activation . ^^^ Here we present evidence that ts 1 infection of astrocytes in vitro activates NF kappaB by enhanced proteolysis of the NF kappaB inhibitors , IkappaBalpha and IkappaBbeta . ^^^ In in vitro studies using protease inhibitors , IkappaBalpha proteolysis in ts 1 infected astrocytes was significantly blocked by a specific calpain inhibitor calpeptin but not by MG 132 , a specific proteasome inhibitor , whereas rapid IkappaBbeta proteolysis was blocked by MG 132 . ^^^ Our results suggest that NF kappaB activation in ts 1 infected astrocytes is mediated by enhanced proteolysis of IkappaBalpha and IkappaBbeta through two different proteolytic pathways , the calpain and ubiquitin proteasome pathways , resulting in increased expression of iNOS , a NF kappaB dependent gene . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The signal progressed through the activation of the IKK complex , the phosphorylation of IkappaBalpha , and the degradation of phosphorylated IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Stimulation with TNF alpha induced a > 10x increase in 1 kappa B kinase ( IKK ) activity that quickly diminished by 20 min . 1 kappa B alpha and 1 kappa B beta proteins were degraded during this time , and 1 kappa B alpha was resynthesized subsequently by NF kappa B dependent transcription . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In this study , it is demonstrated that RelB is associated in the cytosol with p 100 but not with IkappaBalpha , IkappaBbeta , IkappaBepsilon , nor p 105 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Sustained NF kappaB activation by ligands of RAGE was mediated by initial degradation of IkappaB proteins followed by new synthesis of NF kappaBp 65 mRNA and protein in the presence of newly synthesized IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IkappaBbeta , but not IkappaBalpha degradation , induced by IL 1beta was markedly attenuated when ERK activation was inhibited and could be partially responsible for the persistent NF kappaB activation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The kinetics of the expression of proteins , including LMP 1 , 1 kappa B alpha and 1 kappa B beta , was analyzed by Western blotting . ^^^ CONCLUSIONS : The results indicated that in nasopharyngeal carcinoma cells , LMP 1 activated NF kappa B via phosphorylation and degradation of 1 kappa B alpha , but not 1 kappa B beta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The suppression of the NF kappaB activation was accomplished by blocking the dissociation of inhibitory IkappaBalpha and IkappaBbeta by CR . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Detailed examination of the NF kappaB cascade revealed that cardiac cells displayed a unique pattern of IkappaB degradation in response to LPS , with IkappaBbeta but not IkappaBalpha being degraded upon stimulation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In this study , we used immunocytochemistry to investigate the in vivo expression of the p 50 , IkappaBalpha and IkappaBbeta NFkappaB components in endometrium obtained from normal fertile women throughout the menstrual cycle . ^^^ The staining patterns for IkappaBalpha and IkappaBbeta were similar . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IL 1beta produced a prolonged stimulation of NF kappaB / Rel factors by inducing both IkappaBalpha and IkappaBbeta degradation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The suppressive activity of hTid 1 on IKKbeta requires a functional J domain that mediates association with heat shock proteins and results in prolonging the half life of the NF kappaB inhibitors IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The age related decline in the activation of NF kappa B could not be attributed to an alteration in the composition of constituent subunits or degradation of NF kappa B inhibitory proteins ( 1 kappa B alpha and 1 kappa B beta ) but rather was due to selective down regulation of RelA / p65 and NF kappa B2 / p52 proteins . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The impaired NF kappaB activation in Bcr Abl expressing cells was not due to absence of the NF kappaB proteins p 65 , p 50 , or p 100 or of IkappaBalpha or IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Our study reports that infection of cultured astrocytes with TMEV resulted in a time dependent phosphorylation of IkappaBalpha , degradation of IkappaBalpha and IkappaBbeta , activation of NF kappaB and expression of NOS 2 . ^^^ IL 4 and IL 10 caused an accumulation of IkappaBalpha in TMEV infected astrocytes without affecting IkappaBbeta levels . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
These events were preceded by degradation of inhibitors kappaBalpha ( IkappaBalpha ) and kappaBI ( IkappaBbeta ) , and activation of nuclear factor kappaB ( NF kappaB ) in the lung . ^^^ Degradation of IkappaBalpha and activation of NF kappaB in the lung were not altered by endotoxin tolerance , whereas kinetics of IkappaBbeta degradation was only delayed . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Although the abundance of the inhibitor protein IkappaBbeta was higher in 267B1 / Ki ras cells than in 267B1 cells , an electrophoretic mobility shift assay suggested that IkappaBalpha is responsible for the activation of NF kappaB in the former cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Cerulein induces NF kappaB / Rel via activation of IkappaB kinase ( IKK ) , which causes degradation of IkappaBalpha but not IkappaBbeta . ^^^ Tumor necrosis factor alpha mediated IKK activation leads to IkappaBalpha and IkappaBbeta degradation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Endotoxin , but not platelet activating factor , activates nuclear factor kappaB and increases IkappaBalpha and IkappaBbeta turnover in enterocytes . ^^^ We then examined the effect of LPS and TNF on the inhibitory molecules IkappaBalpha and IkappaBbeta . ^^^ We found that TNF causes rapid degradation of IkappaBalpha and IkappaBbeta . ^^^ In contrast , LPS did not change the levels of IkappaBalpha and IkappaBbeta up to 4 hr ( by Western blot ) . ^^^ However , in the presence of cycloheximide , there was a slow reduction of IkappaBalpha and IkappaBbeta , which disappeared almost completely at 4 hr . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IL 10 and IL 13 attenuate NF kappaB activation by interfering with breakdown of IkappaBalpha , while SLPI likewise suppresses NF kappaB activation , but by interfering with breakdown of IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
These results demonstrate the important function of various NF kappaB / IkappaB complexes in regulating anti apoptotic genes in response to apoptotic stimuli , and they raise the possibility that NF kappaB : IkappaBalpha and NF kappaB : IkappaBbeta complexes are regulated by different upstream activators , and that NF kappaB plays a key role in pancreatic tumorigenesis . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The NF kappaB binding proteins IkappaBbeta and IkappaBgamma were prevalent in microsomes , while IkappaBalpha and IkappaB epsilon proteins were absent . ^^^ In cytosol , IkappaBalpha , IkappaBbeta , and IkappaB epsilon , but not IkappaBgamma , were clearly observed but were not changed by TCDD . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Ikappa Bgamma and prototypical Ikappa Bs use a similar mechanism to bind but a different mechanism to regulate the subcellular localization of NF kappa B . p 105 , also known as NF kappaB 1 , is an atypical IkappaB molecule with a multi domain organization distinct from other prototypical IkappaBs , like IkappaBalpha and IkappaBbeta . ^^^ In the case of IkappaBalpha and IkappaBbeta , the specific NF kappaB subunit in the complex is p 65 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Human T lymphotropic virus type 1 tax activates 1 kappa B kinase by inhibiting 1 kappa B kinase associated serine / threonine protein phosphatase 2A . 1 kappa B kinase ( IKK ) is a serine / threonine kinase that phosphorylates 1 kappa B alpha and 1 kappa B beta and targets them for polyubiquitination and proteasome mediated degradation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The model demonstrates that IkappaBalpha is responsible for strong negative feedback that allows for a fast turn off of the NF kappaB response , whereas IkappaBbeta and epsilon function to reduce the system ' s oscillatory potential and stabilize NF kappaB responses during longer stimulations . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Second phase activity correlates with persistent down regulation of both IkappaBalpha and IkappaBbeta proteins , derived from a continuous TNF signal . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NF kappaB activity , as assessed by IkappaBalpha or IkappaBbeta degradation , electrophoretic mobility shift assay and NF kappaB gene reporter assays , is shown to be unaffected by caspase inhibition . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IkappaBalpha and IkappaBbeta are regulators of the nuclear factor kappaB ( NF kappaB ) transcription factor family . ^^^ To better understand how these two IkappaB isoforms differ biologically , we have characterized the expression of IkappaBbeta in testis , a tissue in which IkappaBalpha is only minimally expressed . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NFkappaB translocation from cytoplasm to the nucleus is initiated by its separation from the inhibitory IkappaB proteins which include IkappaBalpha , IkappaBbeta , and IkappaB . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Differential usage of IkappaBalpha and IkappaBbeta in regulation of apoptosis versus gene expression . ^^^ Further analysis showed that while 3 CPA inhibited breakdown of IkappaBalpha , Na 3 CPA inhibited breakdown of IkappaBbeta . ^^^ Taken together , our data implicate distinct roles for IkappaBalpha and IkappaBbeta in regulating various NF kappaB activities . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The expressions of 1 kappa B alpha and 1 kappa B beta decreased to 1 / 3 to 1 / 2 of the normal vein 6 hours to 24 hours after the surgery and then increased to 5 times that of the control vein 2 weeks after surgery ( 35 % + / 11 % and 44 % + / 13 % respectively ) . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The IkappaBalpha and IkappaBbeta proteins inhibit the transcriptional potential of active NF kappaB dimers through stable complex formation . ^^^ It has been shown that inactive IkappaBalpha 10 NF kappaB complexes shuttle in and out of the nucleus , whereas IkappaBbeta 10 NF kappaB complexes are retained exclusively in the cytoplasm of resting cells . ^^^ Deletion of the IkappaBbeta insert renders IkappaBbeta 10 NF kappaB complexes capable of shuttling between the nucleus and cytoplasm , similar to IkappaBalpha 10 NF kappaB complexes . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Treatment with PPARgamma agonists restored IkappaBalpha , IkappaBbeta , and HSP 70 levels to values equal or above those observed in control animals , and reduced activation of cortical NF kappaB . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In this study , inhibitor kappaBalpha ( IkappaBalpha ) and IkappaBbeta protein production was not affected by the chemical protease inhibitors and , more importantly , BCG led to the rapid but transient induction of NF kappaB activity . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Differential phosphorylation of the signal responsive domain of 1 kappa B alpha and 1 kappa B beta by 1 kappa B kinases . ^^^ NF kappa B activity is regulated by its association with the inhibitory 1 kappa B proteins , among which 1 kappa B alpha and 1 kappa B beta are the most abundant . 1 kappa B proteins are widely expressed in different cells and tissues and bind to similar combinations of NF kappa B proteins . ^^^ To better understand the underlying mechanism of the differences in degradation kinetics , we have carried out a systematic , comparative analysis of the ability of the IKK catalytic subunits to phosphorylate 1 kappa B alpha and 1 kappa B beta . ^^^ These findings provide the first systematic analysis of the ability of 1 kappa B alpha and 1 kappa B beta to serve as substrates for IKKs and help provide a possible explanation for the differential degradation kinetics of 1 kappa B alpha and 1 kappa B beta . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We have now generated mice deficient in beta TrCP 1 and shown that the degradation of 1 kappa B alpha and 1 kappa B beta is reproducibly , but not completely , impaired in the cells of these animals . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IMPLICATION OF THE HYPOTHESIS : IkappaBalpha contains the required SUMOylation motif but IkappaBbeta does not . ^^^ The suggested study would provide evidence whether or not IkappaBalpha and IkappaBbeta can substitute each other . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
MEKK 3 is involved in the formation of the IkappaBalpha : NF kappaB / IKK complex , whereas MEKK 2 participates in assembling the IkappaBbeta : NF kappaB / IKK complex ; these two distinct complexes regulate the proinflammatory cytokine induced biphasic NF kappaB activation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Moreover , we observed that two IkappaB subunits ( IkappaBalpha and IkappaBbeta ) were tyrosine phosphorylated after Ang 2 stimulation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
This increase in NF kappaB activation caused by proteasome inhibitors was accompanied by an increase in IkappaB kinase activation and a degradation of IkappaBalpha but not IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Our studies reveal that a commonly used non steroid anti inflammatory drug , acetylsalicylic acid ( aspirin ) prevents H2O2 induced NF kappaB activation in a dose dependent manner through inhibition of phosphorylation and degradation of IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Electrophoretic mobility shift assay ( EMSA ) and Western Blot were used to detect the activation of NF kappaB , nuclear translocation of p 65 subunit and degradation of IkappaBalpha and IkappaBbeta in rat renal tissue . ^^^ Significant up regulation of NF kappaB activation , nuclear translocation of p 65 subunit , and degradation of IkappaBalpha and IkappaBbeta were also observed in NTN rat renal tissue , as compared to the control group . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Inducible activation of the transcription factor NF kappaB ( nuclear factor kappaB ) is classically mediated by proteasomal degradation of its associated inhibitors , IkappaBalpha ( inhibitory kappaBalpha ) and IkappaBbeta . ^^^ However , this sequence was insufficient to target IkappaBbeta to the non proteasome degradation pathway , suggesting that there was an additional cis element ( s ) in IkappaBalpha that was required for complete targeting . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IkappaBalpha and IkappaBbeta possess injury context specific functions that uniquely influence hepatic NF kappaB induction and inflammation . ^^^ Studies evaluating IkappaBbeta knock in mice ( AKBI ) , in which the IkappaBalpha gene is replaced by the IkappaBbeta cDNA , have uncovered divergent properties of IkappaBalpha and IkappaBbeta that influence their ability to activate hepatic NF kappaB and subsequent downstream proinflammatory processes in a stimulus specific manner . ^^^ Molecular studies suggest that the specificity of IkappaBalpha , but not IkappaBbeta , to properly regulate NF kappaB induction during the acute phase of I / R injury is due to injury context specific activation of c Src and subsequent tyrosine phosphorylation of IkappaBalpha on Tyr 42 . ^^^ These results demonstrate that IkappaBalpha and IkappaBbeta play unique injury context specific roles in activating NF kappaB mediated proinflammatory responses and suggest that strategies aimed at inhibiting IkappaBalpha gene expression may be of potential therapeutic benefit in hepatic I / R injury . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Our studies reveal that acetylsalicylic acid ( aspirin ) prevents TNFalpha and IL 1 induced NF kappaB activation in a dose dependent manner through inhibition of phosphorylation and degradation of IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Investigation of the mechanisms of NF kappaB activation following HCMV infection showed a rapid and sustained decrease in the inhibitors of NF kappaB , IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IL 1beta and TNF , alone or in combination with IFNgamma , failed to induce IkappaBalpha or IkappaBbeta degradation in HT 29 cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Furthermore , inhibited NF kappaB activation was characterized by reduced degradation , phosphorylation , or both of IkappaBalpha and IkappaBepsilon but not IkappaBbeta and by reduced phosphorylation of Ser 536 , located in the trans activation domain of p 65 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
IkappaBbeta exhibited marked degradation in response to the excitotoxity , while changes in the levels of IkappaBalpha and p 105 isoforms were not detected . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Chimeric analyses of IkappaBalpha and IkappaBbeta further revealed that the ankyrin repeats of IkappaBalpha , but not IkappaBbeta , contained information necessary for PIR degradation , thereby explaining IkappaBalpha selectivity for the PIR pathway . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Moreover , CHS 828 has also been shown to inhibit the LPS induced degradation of the 1 kappa B alpha and 1 kappa B beta in THP 1 cells , leading us to identify the 1 kappa B kinase complex as a molecular target of CHS 828 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Here we report that trophic factor deprivation in cultured cerebellar granule neurons leads to a rapid and sustained increase in the level of IkappaBalpha and IkappaBbeta , the inhibitory proteins of NF kappaB , causing prolonged NF kappaB inactivation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Because NF kappaB translocates to the nucleus upon the phosphorylation and degradation of inhibitor of IkappaB , we also used Western blots to measure cytosolic protein levels of IkappaBalpha and IkappaBbeta , and the IkappaB kinases , IKKalpha and IKKbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Infection with wild type amastigotes resulted in a time dependent proteolytic degradation of IkappaBalpha and IkappaBbeta and the related protein NF kappaB . ^^^ Cysteine peptidase inhibitors prevented IkappaBalpha , IkappaBbeta , and NF kappaB degradation induced by amastigotes , and recombinant CPB2 . 8 , an amastigote specific isoenzyme of CPB , was shown to degrade GST IkappaBalpha in vitro . ^^^ LPS mediated IkappaBalpha and IkappaBbeta degradation was not affected by these inhibitors , confirming that the site of degradation of IkappaBalpha , IkappaBbeta , and NF kappaB by the amastigotes was not receptor driven , proteosomal mediated cleavage . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Some IkappaB proteins , including IkappaBalpha and IkappaBbeta , can regulate transcription by modulating the concentration of active NF kappaB complexes within the nucleus . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Immunohistochemical evaluation included members of the NF kappaB ( p 50 , p 65 , p 52 , c Rel , Rel B ) and the IkappaB ( IkappaBalpha , IkappaBbeta , IkappaBepsilon , Bcl 3 ) families , as well as putative targets of NF kappaB such as Flip , Bcl xL , Cyclin D 1 , and oestrogen and progesterone receptors . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
METHODS : The effect of VSL # 3 conditioned media on the nuclear factor kappaB pathway in young adult mouse colonic epithelial cells was assessed by using monocyte chemoattractant protein 1 enzyme linked immunosorbent assays ; IkappaBalpha , IkappaBbeta , and p 105 immunoblot analysis ; and nuclear factor kappaB luciferase reporter gene and proteasome assays . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Ethyl pyruvate had no effect on the degradation of IkappaBalpha or IkappaBbeta in lipopolysaccharide stimulated RAW 264 . 7 cells , suggesting that ethyl pyruvate acts distally to this step in the activation of NF kappaB . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Finally , we found that TCR / CD28 costimulation induces IkappaBalpha , IkappaBbeta , and IkappaBepsilon degradation , and PKC is required for IkappaBalpha and IkappaBepsilon but not IkappaBbeta degradation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
PS 341 treatment of these cells stabilized IkappaBalpha , IkappaBbeta , IkappaBvarepsilon , p 21 , p 27 and p 53 proteins and selectively inhibited Rel A DNA binding NF kappaB complexes . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Whereas NFATc 1 expression was increased significantly at days 3 and 5 following RANK L exposure , there were no significant increases in the expression of NFkappaB subunits , namely p 65 , p 50 , c Rel , IkappaBalpha , and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Subsequent coimmunoprecipitation experiments revealed that , in addition to IkappaBbeta , other IkappaB family members examined ( p 105 , p 100 , and IkappaBalpha ) also associated with PDE8A1 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Coexpression of IkappaBalpha , IkappaBbeta , IKKalpha , IKKbeta , NIK and TRAF 2 dominant negative constructs , with LMP 1 inhibited the activation of the CCL 5 promoter by LMP 1 , suggesting that LMP 1 induces CCL 5 via NF kappaB signaling . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Ethacrynic acid inhibited degradation of IkappaBalpha and IkappaBbeta in lipopolysaccharide stimulated RAW 264 . 7 cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
To elucidate the underlying mechanisms , we investigated the expression of NF kappaB subunits , p 65 and p 50 , and IkappaB proteins , IkappaBalpha and IkappaBbeta . ^^^ The transcript and protein levels of p 50 , p 65 , and IkappaBbeta remained relatively unchanged during the course of infection , but Ser 32 phosphorylation of IkappaBalpha at 3 h was significantly increased over the basal level in uninfected cells concomitant with a significant increase in the expression of IkappaBalpha mRNA . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The predicted amino acid sequence is 61 . 5 % similar and 54 % identical to human IkappaBalpha , while only 42 % similar and 35 % identical to IkappaBbeta , and 38 % similar and 32 % identical to IkappaBvarepsilon . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The levels and stability of IkappaBepsilon have been examined in unstimulated and stimulated splenic B cells and compared with that of IkappaBalpha and IkappaBbeta . ^^^ Most agents that activate IkappaBalpha and IkappaBbeta degradation do not induce rapid degradation of IkappaBepsilon . ^^^ Interestingly , however , the levels of IkappaBepsilon , but not of IkappaBalpha or IkappaBbeta , are dramatically reduced upon the stimulation of B cells both in vivo and in vitro . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Interestingly , PPARgamma agonists induced an increase in IkappaBalpha and IkappaBbeta protein levels , which was prevented with CD 40 engagement . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Through the utilization of immunoprecipitation techniques , protein protein interactions between NF kappaB family members IkappaBalpha , IkappaBbeta , p 50 , and p 65 ( Rel A ) were identified with an Xpress tagged dominant negative c Jun ( TAM 67 ) protein . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Ang 2 treatment did not increase phosphorylation of inhibitor of kappaBalpha ( IkappaBalpha ) or IkappaBbeta or decrease their levels . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In contrast to the defined regulatory interplay between NF kappaB and IkappaBalpha , much less is known regarding the regulation of IkappaBbeta by NF kappaB . ^^^ Significantly , in both p 65 ( / ) cells and tissues , IkappaBalpha is also reduced , but not nearly to the same extent as IkappaBbeta , thus highlighting the degree to which IkappaBbeta is dependent on p 65 . ^^^ Finally , we show that in p 65 ( / ) fibroblasts , expression of a proteolysis resistant form of IkappaBbeta , but not IkappaBalpha , causes a severe growth defect associated with apoptosis . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The alteration in NF kappaB DNA binding activity was neither accompanied by any change in the expression of the inhibitor kappaB ( IkappaB ) kinases , IKKalpha , IKKbeta , and IKKgamma nor in the expression of the NF kappaB inhibitor proteins , IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
This process consists of an initial , IkappaBalpha mediated transient phase and a later , persistent phase dependent on IkappaBbeta degradation . ^^^ This ability was attributed to blockage of the persistent phase of TNF alpha induced NF kappaB activation for the following reasons : ( 1 ) the initial phase of NF kappaB transcriptional activation was not affected by the MC 159 protein ; ( 2 ) the MC 159 protein inhibited TNF alpha directed degradation of IkappaBbeta , but not IkappaBalpha ; and ( 3 ) expression of the late NF kappaB regulated cell genes , TNF alpha and CCL 2 , was decreased in the presence of the MC 159 protein while transcription of the early NF kappaB regulated cell gene , CXCL 1 , was not altered . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
CoMTsAg induced maximal nuclear binding of NF kappaB p 65 and p 50 by 1 h , with IkappaBalpha and IkappaBbeta decay within 15 min . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
We show that in cells depleted of IkappaBalpha , IkappaBbeta and IkappaBepsilon proteins , a small fraction of p 65 binds DNA and leads to constitutive activation of NF kappaB target genes , even without stimulation , whereas most of the p 65 remains cytoplasmic . ^^^ These results indicate that although IkappaBalpha , IkappaBbeta and IkappaBepsilon proteins could be dispensable for cytoplasmic retention of NF kappaB , they are essential for preventing NF kappaB dependent gene expression in the basal state . ^^^ We also show that in the absence of IkappaBalpha , IkappaBbeta and IkappaBepsilon proteins , cytoplasmic retention of NF kappaB by other cellular proteins renders the pathway unresponsive to activation . . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Thus , poly 1 induced an increase in nuclear factor kappaB ( NF kappaB ) transcriptional activity due to a long term degradation of inhibitory NF kappaB ( IkappaB ) beta while lipopolysaccharide ( LPS ) induced the degradation of both IkappaBalpha and IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Eight weeks later we measured TBARS and hydroperoxide initiated chemiluminescence ( QL ) in liver as markers of oxidative stress , and activities of the antioxidant enzymes catalase , superoxide dismutase ( SOD ) , and glutathione peroxidase , NF kappaB activation by an electrophoretic mobility shift assay and expression of IkappaB kinases ( IKKalpha and IKKbeta ) , the inhibitor IkappaB ( IkappaBalpha and IkappaBbeta ) , and iNOS by Western blot . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The enhanced activation of NF kappaB by resveratrol progressed for at least 24 h and was accompanied by sustained down regulation of an endogenous NF kappaB inhibitor , IkappaBbeta , but not IkappaBalpha . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Moreover , equol blocked degradation of IkappaBalpha and IkappaBbeta and nuclear translocation of p 65 subunit of NF kappaB in activated RAW 264 . 7 cells . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Ang 2 also decreased the cytosolic level of the inhibitor of NF kappaB ( IkappaB ) and increased the phosphorylation of IkappaB subunits , IkappaBalpha and IkappaBbeta , as well as the phosphorylation of IkappaB kinase ( IKK ) subunits , IKKalpha and IKKbeta , suggesting that Ang 2 enhanced the breakdown of IkappaB . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
However , NFkappaB activation occurred without degradation of inhibitory IkappaB proteins ( IkappaBalpha , IkappaBbeta , and IkappaBepsilon ) . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
In these cells , NFkappaB retains DNA binding activity for up to 72 h despite the presence of resynthesized IkappaBalpha and in the absence of IkappaBbeta . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
APDC was actually confirmed to suppress the degradation of IkappaBalpha and IkappaBbeta in microglia , indicating a role for the inhibitor of NFkappaB activation . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
Ectopic expression of the Bcl 3 related molecules IkappaBalpha , IkappaBbeta , and IkappaBepsilon in SEB activated T cells increased survival during in vitro culture in the absence of adjuvant , suggesting that these IkappaB molecules could exert a survival function in antigen activated T cells in place of Bcl 3 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
The classical NF kappaB pathway activates the IKK complex that controls the inducible degradation of most IkappaB family members that are IkappaBalpha , IkappaBbeta , IkappaBvarepsilon and p 105 . ^^^
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
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Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q15653 and P25963	Pubmed	SVM Score :0.0
NA