| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Regulation of NF kappa B activity by 1 kappa B alpha and 1 kappa B beta stability . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The increase in NF kappa B binding activity is accompanied by a slow and steady decrease in 1 kappa B beta protein levels . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NF kappa B is activated following degradation of 1 kappa B alpha and 1 kappa B beta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| We report here that NF kappa B and 1 kappa B alpha ( but not 1 kappa B beta ) are also localized in the mitochondria . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Furthermore , it was found that PDE8A1 competed with the p65 / p50 NF kappaB for IkappaBbeta binding . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| There was no evidence for regulation by IkappaBbeta or the alternate pathway of NF kappaB activation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.74086766 |
| Phosphatase treatment also released active NF kappa B from its inactive complex with 1 kappa B beta suggesting that the activation of NF kappa B in intact cells might not only rely on phosphate transfer onto 1 kappa B but also on phosphate removal from one form of 1 kappa B . . 0.74086766^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.64212035 |
| The unphosphorylated 1 kappa B beta forms a stable complex with NF kappa B in the cytosol ; however , this binding fails to mask the nuclear localization signal and DNA binding domain on NF kappa B , and the 1 kappa B beta NF kappa B complex enters the nucleus . 0.64212035^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| A deletion analysis of the p 50 subunit revealed that transcriptional activation was mediated by the conserved c rel related domain . 1 kappa B beta ( or a related protein ) , which binds to the p 65 but not the p 50 subunit of NF kappa B , inhibited stimulation by natural NF kappa B but not by recombinant p 50 . ^^^ Combined with DNA binding experiments , these studies suggest a role of p 50 homodimers in transcriptional activation of certain promoters , with a possible preference for those carrying symmetric NF kappa B recognition sites , and a potential role of 1 kappa B beta in direct transcriptional regulation within the nucleus . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The rel associated pp 40 protein prevents DNA binding of Rel and NF kappa B : relationship with 1 kappa B beta and regulation by phosphorylation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Transcription factors belonging to the NF kappa B family are controlled by inhibitory 1 kappa B proteins , mainly 1 kappa B alpha and 1 kappa B beta . ^^^ In contrast to hematopoietic cells , 1 kappa B alpha / embryonic fibroblasts show minimal constitutive NF kappa B , as well as normal signal dependent NF kappa B activation that is concomitant with 1 kappa B beta degradation . ^^^ Our results indicate that 1 kappa b beta , but not 1 kappa B alpha , is required for the signal dependent activation of NF kappa B in fibroblasts . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Instead the primary difference between 1 kapp B alpha and 1 kappa B beta is in their response to different inducers of NF kappa B activity . ^^^ One class of inducers causes rapid but transient activation of NF kappa B by primarily affecting 1 kappa B alpha complexes , whereas another class of inducers causes persistent activation of NF kapa B by affecting both 1 kappa B alpha and 1 kappa B beta complexes . ^^^ Therefore , the overall activation of NF kappa B consists of two overlapping phases , a transient phase mediated through 1 kappa B alpha and a persistent phase mediated through 1 kappa B beta . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Inactivation of IkappaBbeta by the tax protein of human T cell leukemia virus type 1 : a potential mechanism for constitutive induction of NF kappaB . ^^^ In resting T lymphocytes , the transcription factor NF kappaB is sequestered in the cytoplasm via interactions with members of the 1 kappa B family of inhibitors , including IkappaBalpha and IkappaBbeta . ^^^ Despite the dual specificity of HTLV 1 Tax for IkappaBalpha and IkappaBbeta at the protein level , Tax selectively stimulates NF kappaB directed transcription of the IkappaBalpha gene . ^^^ These findings with IkappaBbeta provide a potential mechanism for the constitutive activation of NF kappaB in Tax expressing cells . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Cultured fibroblasts derived from IkappaBalpha deficient embryos exhibit levels of NF kappaB 1 , NF kappaB 2 , RelA , c Rel , and IkappaBbeta similar to those of wild type fibroblasts . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Elevated levels of NF kappa B were partially achieved through a sustained decrease in the steady state amount of the NF kappa B / Rel specific inhibitory protein , 1 kappa B alpha , and a transient decrease in 1 kappa B beta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| In most cell types other than mature B lymphocytes and macrophages , the transcription factor NF kappaB remains in an inactive form in the cytosol by being bound to the inhibitory proteins IkappaBalpha and IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Signal initiated activation of the transcription factor NF kappaB is mediated through proteolysis of its cytoplasmic inhibitory proteins IkappaBalpha and IkappaBbeta . ^^^ While most NF kappaB inducers trigger the degradation of IkappaBalpha , only certain stimuli are able to induce the degradation of IkappaBbeta . ^^^ We show that the serine / threonine phosphatase inhibitor calyculin A induces the hyperphosphorylation and subsequent degradation of IkappaBbeta in both human Jurkat T cells and the murine 70Z 3 preB cells , which is associated with the nuclear expression of active NF kappaB . ^^^ These results suggest that the degradation signal of IkappaBbeta may be controlled by the opposing actions of protein kinases and phosphatases and that both the N and C terminal sequences of IkappaBbeta are required for the inducible degradation of this NF kappaB inhibitor . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NF kappaB 1 translocation depends on simultaneous proteolysis of both IkappaBalpha and IkappaBbeta isoforms ; IkappaBgamma is inert to TNFalpha treatment . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Upon treatment with a large variety of inducers , IkappaBalpha and IkappaBbeta are proteolytically degraded , resulting in NF kappaB translocation into the nucleus . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Unlike 1 kappa B alpha and 1 kappa B beta , 1 kappa B epsilon does not contain a C terminal PEST like sequence . 1 kappa B epsilon would , therefore , appear to regulate a late , transient activation of a subset of genes , regulated by RelA / cRel NF kappa B complexes , distinct from those regulated by other 1 kappa B proteins . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Degradation of IkappaBbeta and the translocation of the NF kappaB ( p50 / RelA ) into the nucleus , which occurred at 1 . 5 hr after anti CD 3 activation , were inhibited by lactacystin . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| CsA does not interfere with the synthesis of Rel proteins , but prevents the inducible degradation of cytosolic NF kappa B inhibitors 1 kappa B alpha and 1 kappa B beta upon T cell activation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Listeria monocytogenes infection of P388D1 macrophages results in a biphasic NF kappaB ( RelA / p50 ) activation induced by lipoteichoic acid and bacterial phospholipases and mediated by IkappaBalpha and IkappaBbeta degradation . ^^^ We suggest that products of the enzymatic activity of phospholipases directly interfere with host cell signal transduction pathways , thus leading to persistent NF kappaB activation via persistent IkappaBbeta degradation . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of the PEST domain of IkappaBbeta regulates the function of NF kappaB / IkappaBbeta complexes . ^^^ Activation of transcription factor NF kappaB involves the signal dependent degradation of basally phosphorylated inhibitors such as IkappaBalpha and IkappaBbeta . ^^^ However , recent studies have identified a hypophosphorylated pool of IkappaBbeta that shields nuclear NF kappaB from inhibition by newly synthesized IkappaBalpha . ^^^ First , disruption of two basal phosphoacceptors present in the IkappaBbeta PEST domain ( Ser 313 and Ser 315 ) yields a mutant that forms ternary complexes with NF kappaB and its target DNA binding site . ^^^ Third , mutants of IkappaBbeta that are defective for phosphorylation at Ser 313 and Ser 315 fail to efficiently block NF kappaB directed transcription in vivo , whereas replacement of these two IkappaBbeta residues with a phosphoserine mimetic generates a fully functional repressor . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Rapid Up regulation of IkappaBbeta and abrogation of NF kappaB activity in peritoneal macrophages stimulated with lipopolysaccharide . ^^^ The levels of IkappaBalpha and IkappaBbeta were analyzed during this period in an attempt to correlate NF kappaB activity with IkappaB resynthesis . ^^^ IkappaBbeta degradation , which has been associated with persistent NF kappaB activation , was complete at 1 h . ^^^ However , a rapid recovery of IkappaBbeta in these tissues was observed at 3 h in parallel with the abrogation of NF kappaB activity . ^^^ These results suggest the existence of LPS and tumor necrosis factor alpha specific pathways involved in a rapid IkappaBbeta degradation and resynthesis and might explain the transient period of activation of NF kappaB in these tissues upon stimulation with these factors . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Analysis of the kappaB inhibitory ( 1 ) proteins showed an important role for IkappaBalpha in the process of NF kappaB activation , whereas the contribution of IkappaBbeta was less evident . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The NF kappa B family members p 50 , p 65 , p 52 , c Rel , and RelB as well as the inhibitor proteins 1 kappa B alpha , 1 kappa B beta , and p 105 were present in uninjured arteries as determined by immunoblotting . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| When activated by immobilized anti CD 3 monoclonal antibody , hybridoma T cells ( 5D5 ) degraded 1 kappa B beta , translocated NF kappa B into the nucleus , transcribed immediate early genes and the Fas ligand ( FasL ) gene , and expressed FasL mediated cytotoxicity . ^^^ Lactacystin strongly blocked 1 kappa B beta degradation and the translocation of NF kappa B ( p50 / RelA heterodimer ) , but had little effect on the expression of the transcription factors , Oct 1 and AP 1 . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Proteasome dependent inhibitory kappa B alpha ( 1 kappa B alpha ) and 1 kappa B beta degradation occurred downstream of CD 40 ligation and preceded CD 40 mediated NF kappa B nuclear translocation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Transfection with a plasmid containing cDNA encoding mouse IkappaBbeta , an inhibitor of NF kappaB , resulted in increased toxicity by menadione . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The biological activity of the transcription factor NF kappaB is mainly controlled by the IkappaB proteins IkappaBalpha and IkappaBbeta , which restrict NF kappaB in the cytoplasm and enter the nucleus where they terminate NF kappaB dependent transcription . ^^^ In addition , IkappaBepsilon appears to function predominantly in the cytoplasm to sequester p 65 homodimers , in contrast with the other two members of the family , IkappaBalpha and IkappaBbeta , which also function in the nucleus to terminate NF kappaB dependent transcriptional activation . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Sesquiterpene lactones specifically inhibit activation of NF kappa B by preventing the degradation of 1 kappa B alpha and 1 kappa B beta . ^^^ Treatment of cells with SLs prevented the induced degradation of 1 kappa B alpha and 1 kappa B beta by all these stimuli , suggesting that they interfere with a rather common step in the activation of NF kappa B . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Control of NF kappa B activity by the 1 kappa B beta inhibitor . ^^^ In mammalian cells , 1 kappa B alpha and 1 kappa B beta proteins have been purified and shown to be the inhibitors of NF kappa B through their association with the p 65 or c Rel subunits . ^^^ Upon treatment with a large variety of inducers , 1 kappa B alpha , 1 kappa B beta are proteolytically degraded , resulting in NF kappa B translocation into the nucleus . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Overexpression of TAK 1 together with its activator protein , TAK 1 binding protein 1 ( TAB 1 ) , induced the nuclear translocation of NF kappa B p50 / p65 heterodimer accompanied by the degradation of 1 kappa B alpha and 1 kappa B beta , and the expression of kappa B dependent reporter gene . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| However , in contrast to previous reports , prolonged nuclear localization of NF kappaB complexes is not necessarily associated with long term depletion of IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| During persistent activation of NF kappaB at later times in infection , syntheses of inhibitors IkappaBalpha as well as IkappaBbeta were restored . ^^^ However , the resynthesized IkappaBbeta was in an underphosphorylated state , which apparently prevented inhibition of NF kappaB . ^^^ Thus , RSV infection led to the activation of NF kappaB by a biphasic mechanism : a transient or early activation involving phosphorylation of the inhibitor IkappaB polypeptides , and a persistent or long term activation requiring PKC and the generation of hypophosphorylated IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| DNA PK also phosphorylated the NF kappa B inhibitory proteins IkB alpha and IkB beta in vitro , and deletion analysis demonstrated that DNA PK phosphorylates 2 distinct regions of IkB beta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Here we present evidence that beginning at around 6 h postinfection , herpes simplex virus ( HSV ) induces a persistent translocation of NF kappa B into the nucleus of C 33 cells , coincident with loss of both 1 kappa B alpha and 1 kappa B beta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| In addition , overexpression of the dominant negative mutants of NF kappaB inhibitor molecules , IkappaBalpha and IkappaBbeta , abolished the Tax induced activation of IL 8 gene . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Lipopolysaccharide induced NF kappaB activation in human endothelial cells involves degradation of IkappaBalpha but not IkappaBbeta . ^^^ We conclude that in HUVEC , LPS induces NF kappaB dependent genes through degradation of IkappaBalpha , not IkappaBbeta , and propose that this degradation is induced in part by HMA sensitive kinase ( s ) upstream of IkappaBalpha . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| METHODS : The expression of NF kappa B subunits p 65 and p 50 and the inhibitory subunits 1 kappa B alpha and 1 kappa B beta in human and rat myocardial samples was measured by immunoblotting , using antibodies , specific to each subunit . ^^^ CONCLUSIONS : The principal NF kappa B subunits p 65 , p 50 , 1 kappa B alpha and 1 kappa B beta are present throughout development , suggesting that this transcription complex may participate in myocardial gene regulation throughout development and in the adult . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of the IkappaB cytoplasmic inhibitors , IkappaBalpha , IkappaBbeta , and IkappaBepsilon , by these kinases triggers proteolytic degradation and the release of NF kappaB / Rel proteins into the nucleus . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Swapping a putative beta turn within the first ankyrin repeat between the strong Ikappa Balpha and the weak IkappaBbeta inhibitors switches their in vivo inhibitory activity on NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Dexamethasone injection induced the expression of IkappaBalpha and IkappaBbeta in thymocytes and down regulated NF kappaB DNA binding activated by intrathymic signals . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Inhibition of NF kappaB activation and augmentation of IkappaBbeta by secretory leukocyte protease inhibitor during lung inflammation . ^^^ In the inflamed lungs , inhibition of NF kappaB activation by SLPI was associated with elevated levels of lung IkappaBbeta ( but not IkappaBalpha ) protein in the absence of elevated mRNA for IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Within minutes , LPS through CD 14 induced the activation of NF kappaB , degradation of IkappaBalpha ( inhibitory subunit of NF kappaB ) and IkappaBbeta , and nuclear translocation of p 65 . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The mechanism by which these two cytokines exert their synergistic effect on NF kappaB involves the de novo degradation of the NF kappaB inhibitor , IkappaBbeta . ^^^ Our results suggest that the signal generated by IFN gamma during TNF alpha / IFN gamma cotreatment may require PKR to elicit enhanced NF kappaB activity , and this signal may affect the stability of the IkappaBbeta protein . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| We have examined the consequences of overexpression of the IkappaBalpha and IkappaBbeta inhibitory proteins on the regulation of NF kappaB dependent beta interferon ( IFN beta ) gene transcription in human cells after Sendai virus infection . ^^^ Inhibition of IFN beta expression directly correlated with a reduction in the binding of NF kappaB ( p 50 RelA ) complex to PRDII after Sendai virus infection in IkappaBalpha expressing cells , whereas IFN beta expression and NF kappaB binding were only slightly reduced in IkappaBbeta expressing cells . ^^^ These experiments demonstrate a major role for IkappaBalpha in the regulation of NF kappaB induced IFN beta gene activation and a minor role for IkappaBbeta in the activation process . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Unexpectedly , the presence of nuclear 1 ( kappa ) B ( alpha ) did not inhibit NF kappaB binding to DNA and this phenomenon was not due to the presence of IkappaBbeta at the nuclear level . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Persistent HIV infection of these cells results in increased levels of NF kappaB in the nucleus secondary to increased IkappaBalpha , IkappaBbeta , and IkappaBepsilon degradation , a mechanism postulated to regulate viral persistence . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Nuclear IkappaBbeta maintains persistent NF kappaB activation in HIV 1 infected myeloid cells . ^^^ Because IkappaBbeta has been implicated in maintaining NF kappaB . ^^^ DNA binding , we sought to investigate whether IkappaBbeta was involved in maintaining persistent NF kappaB activation in HIV 1 infected monocytic cell lines . ^^^ IkappaBbeta was present in the nucleus of HIV 1 infected cells and participated in the ternary complex formation with NF kappaB and DNA . ^^^ DNA complexes from HIV 1 infected cell extracts did not completely dissociate the complexes , suggesting that IkappaBbeta may protect NF kappaB complexes from IkappaBalpha mediated dissociation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Role of IkappaBalpha and IkappaBbeta in the biphasic nuclear translocation of NF kappaB in TNFalpha stimulated astrocytes and in neuroblastoma cells . ^^^ In infectious diseases of the central nervous system astrocytes respond to inflammatory cytokines like tumor necrosis factor alpha ( TNFalpha ) by activation of the transcription factor NF kappaB , mediated by the proteolysis of its inhibitors IkappaBalpha and IkappaBbeta . ^^^ However , the second peak occurred also in the absence of IkappaBbeta proteolysis , demonstrating the importance of IkappaBalpha in the formation of the biphasic nuclear translocation of NF kappaB . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Treatment of cultured peritoneal macrophages with IFN gamma resulted in tyrosine phosphorylation of IkappaBalpha and IkappaBbeta , NF kappaB activation , and expression of inducible NO synthase ( iNOS ) . ^^^ Treatment with POV of macrophages stimulated with IFN gamma or LPS potentiated the degradation of IkappaBalpha and IkappaBbeta , the activation of NF kappaB , and the expression of iNOS . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| PNU 156804 inhibition of NF kappa B activation is due to the inhibition of the degradation of 1 kappa B alpha and 1 kappa B beta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Negative regulation of transactivation function but not DNA binding of NF kappaB and AP 1 by IkappaBbeta 1 in breast cancer cells . ^^^ To understand the mechanisms of constitutive NF kappaB activation , we have analyzed the function of IkappaBalpha and IkappaBbeta in breast cancer cells . ^^^ In most cases , constitutive NF kappaB DNA binding correlated with reduced levels of either IkappaBalpha or IkappaBbeta isoforms . ^^^ Overexpression of IkappaBalpha but not IkappaBbeta 1 resulted in reduced constitutive DNA binding of NF kappaB in MDA MB 231 cells . ^^^ Unexpectedly , IkappaBbeta 1 overexpression moderately increased 12 O tetradecanoylphorbol 13 acetate and interleukin 1 inducible NF kappaB DNA binding . 12 O Tetradecanoylphorbol 13 acetate and interleukin 1 induced transactivation by NF kappaB , however , was lower in IkappaBbeta 1 overexpressing cells . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| TPCK inhibited constitutive NF kappaB activation in T . parva transformed T cells , with phosphorylation of IkappaBalpha and IkappaBbeta being inhibited with different kinetics . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| RESULTS : NF kappaB activation triggered by PMA was not associated with the disappearance of immunoreactive IkappaBalpha , IkappaBbeta , IkappaBgamma , or IkappaBepsilon or with the dissociation of intact IkappaB from RelA . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Taken together , DZA promotes the proteolytic degradation of IkappaBalpha , but not IkappaBbeta , resulting in an increase of DNA binding activity of NF kappaB in the nucleus in the absence of its transcriptional activity in RAW 264 . 7 cells . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| We show that while this `` classical ' ' mode of NF kappa B activation is a uniform feature of IgM+ B cell lines , all IgG+ B cells analyzed contain nuclear NF kappa B yet have stable 1 kappa B alpha , 1 kappa B beta , and 1 kappa B epsilon . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| This was not explained by degradation of IkappaBbeta , IkappaBepsilon , or p 105 nor nuclear translocation of NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NF kappaB heterodimers reside in the cytoplasm of cells bound to inhibitory proteins , the two commonest of which are IkappaBalpha and IkappaBbeta , which prevent NF kappaB from entering the nucleus . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Tax expression triggers persistent phosphorylation and degradation of the NF kappaB inhibitory proteins IkappaBalpha and IkappaBbeta , resulting in constitutive nuclear expression of NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The time dependent fall of the mRNA levels of NOS 2 and COX 2 paralleled the inhibition of NF kappaB , determined by electrophoretic mobility shift assays , and the increase of the IkappaBalpha and IkappaBbeta inhibitory proteins . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| This transcription factor is regulated by a family of structurally related inhibitors including IkappaBalpha , IkappaBbeta , and IkappaBepsilon , which trap NF kappaB in the cytoplasm . ^^^ LPS activated IKK complexes phosphorylate all 3 inhibitors of NF kappaB : IkappaBalpha , IkappaBbeta , and IkappaBepsilon . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Therefore , these results indicate that beta TrCP plays a critical role in the activation of NF kappaB by assembling the ubiquitin ligase complex for both phosphorylated IkappaBalpha and IkappaBbeta . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The release of NF kappa B factors to the nucleus requires phosphorylation and degradation of the inhibitory kappa B proteins ( 1 kappa Bs ) . 1 kappa B alpha and 1 kappa B beta phosphorylation is dependent on dual signaling by the TCR and the CD 28 accessory receptor . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The molecular mechanism mediating this effect appears to involve repression of NF kappaB activation at the level of IkappaBalpha and IkappaBbeta degradation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Our data show that TNF alpha production by LPS stimulated AMs from asymptomatic HIV seropositive and seronegative individuals is regulated via the phospholipase C pathway and by NF kappa B DNA binding activity without obvious changes in 1 kappa B alpha or 1 kappa B beta protein concentrations . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Here we show that in response to poly ( rI ) . poly ( rC ) ( pIC ) , PKR activates IkappaB kinase ( IKK ) , leading to the degradation of the inhibitors IkappaBalpha and IkappaBbeta and the concomitant release of NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| These proteins , kappaB Ras 1 and kappaB Ras 2 , interact with the PEST domains of IkappaBalpha and IkappaBbeta [ inhibitors of the transcription factor nuclear factor kappa B ( NF kappaB ) ] and decrease their rate of degradation . ^^^ In cells , kappaB Ras proteins are associated only with NF kappaB : IkappaBbeta complexes and therefore may provide an explanation for the slower rate of degradation of IkappaBbeta compared with IkappaBalpha . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| In vascular endothelial cells , 1 kappa B epsilon associates predominantly with the NF kappa B subunit Rel A and to a lesser extent with c Rel , whereas 1 kappa B alpha and 1 kappa B beta associate with Rel A only . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| IkappaBbeta is a member of the IkappaB family of structurally related proteins that are important regulators of the inducible transcription factor NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| We have applied these principles in order to form crystals of the Rel homology region of transcription factor NF kappaB in complex with its inhibitors IkappaBalpha and IkappaBbeta . ^^^ IkappaBbeta complex crystals were formed by analogy to NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| In control C57BL / 6 mice , expression levels of p 65 , IkappaBalpha , and IkappaBbeta were 5 to 18 fold higher in the HP region , yet NF kappaB was activated in a minority of endothelial cells . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Cell fractionation studies were consistent with IkappaBbeta being a major regulator of p 65 p50 NF kappaB complexes in HT 29 cells . ^^^ We propose that deficiencies in the proteasomal degradation of IkappaBbeta lead to p 106 and p 112 accumulation , which in turn alter NF kappaB regulation in colon cancer cells . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| We then delineated the pathway through which 2 AAF activates NF kappa B . 2 AAF treatment led to the increase of intracellular reactive oxygen species ( ROS ) which causes activation of IKK kinases , degradation of 1 kappa B beta ( but not 1 kappa B alpha ) , and increase in NF kappa B DNA binding activity . ^^^ Based on these results , we conclude that 2 AAF up regulates mdr1b through the generation of ROS , activation of IKK kinase , degradation of 1 kappa B beta , and subsequent activation of NF kappa B . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Activation of nuclear factor kappa B ( NF kappa B ) was demonstrated by electrophoretic mobility shift assay , presence of inhibitory kappa B alpha and beta ( 1 kappa B alpha and 1 kappa B beta ) by Western blot analysis , and TNF alpha protein production by enzyme linked immunosorbent assay . ^^^ Elastase , carboxypeptidase A , and lipase induced degradation of 1 kappa B beta ( but not 1 kappa B alpha ) , activation of NF kappa B , and production of TNF alpha protein , whereas inhibition of 1 kappa B with pyrrolidine dithiocarbamate attenuated this response . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Pretreatment ( for 1 h ) of murine macrophages or rat aortic smooth muscle cells ( activated with endotoxin ) with calpain inhibitor 1 attenuated the binding of activated NF kappaB to DNA and the degradation of IkappaBalpha , IkappaBbeta , and IkappaBvarepsilon . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The effect correlates with the rapid degradation of IkappaBalpha , a sustained degradation of IkappaBbeta and increases in tumour necrosis factor alpha ( TNF alpha ) and interleukin ( IL ) 6 production , two cytokines controlled by NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| TD IkappaBalpha , IkappaBbeta , and IkappaBepsilon were not degraded upon TNF alpha exposure , and each prevented NF kappaB activation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| To evaluate the role of the NF kappaB signaling pathway in oncogenic transformation , we expressed IkappaBbeta , a specific inhibitor of NF kappaB , in two human lung adenocarcinoma cell lines , A 549 and H 441 . ^^^ Expression of IkappaBbeta significantly reduced NF kappaB activation induced by cotransfection with p65 / RelA or TNF alpha and abrogated the basal NF kappaB activity in A 549 cells . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| IkappaBbeta , but not IkappaBalpha , functions as a classical cytoplasmic inhibitor of NF kappaB dimers by masking both NF kappaB nuclear localization sequences in resting cells . ^^^ In this study , we have combined in vitro biochemical and cell based methods to elucidate differences in NF kappaB regulation exhibited by the inhibitors IkappaBbeta and IkappaBalpha . ^^^ We show that although both IkappaBalpha and IkappaBbeta bind to NF kappaB with similar global architecture and stability , significant differences exist that contribute to their unique functional roles . ^^^ IkappaBbeta derives its high affinity toward NF kappaB dimers by binding to both NF kappaB subunit nuclear localization signals . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Similar to other NF kappaB inducers , CpG ODN caused an early IkappaBalpha and IkappaBbeta degradation plus cleavage of the p 50 precursor and subsequent NF kappaB nuclear translocation . ^^^ As inhibitory S ODN did not directly interfere with the NF kappaB DNA binding but prevented CpG induced NF kappaB nuclear translocation of p 50 , p 65 , and c Rel and blocked p 105 , IkappaBalpha , and IkappaBbeta degradation , we concluded that their putative target must lie upstream of inhibitory kinase ( IKK ) activation . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Enhanced proteolysis of IkappaBalpha and IkappaBbeta proteins in astrocytes by Moloney murine leukemia virus ( MoMuLV ) ts 1 infection : a potential mechanism of NF kappaB activation . ^^^ Here we present evidence that ts 1 infection of astrocytes in vitro activates NF kappaB by enhanced proteolysis of the NF kappaB inhibitors , IkappaBalpha and IkappaBbeta . ^^^ Our results suggest that NF kappaB activation in ts 1 infected astrocytes is mediated by enhanced proteolysis of IkappaBalpha and IkappaBbeta through two different proteolytic pathways , the calpain and ubiquitin proteasome pathways , resulting in increased expression of iNOS , a NF kappaB dependent gene . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Stimulation with TNF alpha induced a > 10x increase in 1 kappa B kinase ( IKK ) activity that quickly diminished by 20 min . 1 kappa B alpha and 1 kappa B beta proteins were degraded during this time , and 1 kappa B alpha was resynthesized subsequently by NF kappa B dependent transcription . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Sustained NF kappaB activation by ligands of RAGE was mediated by initial degradation of IkappaB proteins followed by new synthesis of NF kappaBp 65 mRNA and protein in the presence of newly synthesized IkappaBalpha and IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| IkappaBbeta , but not IkappaBalpha degradation , induced by IL 1beta was markedly attenuated when ERK activation was inhibited and could be partially responsible for the persistent NF kappaB activation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The elevated NF kappaB activity could be correlated with a reduced level of cytoplasmic IkappaBbeta and could be associated with the overexpression of p 21 ( CIP1 / WAF1 ) in papilloma cells . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The steady state level of total 1 kappa B beta protein may have resulted from the initiation of an autoregulation loop after the activation of NF kappa B . ^^^ CONCLUSIONS : The results indicated that in nasopharyngeal carcinoma cells , LMP 1 activated NF kappa B via phosphorylation and degradation of 1 kappa B alpha , but not 1 kappa B beta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The suppression of the NF kappaB activation was accomplished by blocking the dissociation of inhibitory IkappaBalpha and IkappaBbeta by CR . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Detailed examination of the NF kappaB cascade revealed that cardiac cells displayed a unique pattern of IkappaB degradation in response to LPS , with IkappaBbeta but not IkappaBalpha being degraded upon stimulation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The suppressive activity of hTid 1 on IKKbeta requires a functional J domain that mediates association with heat shock proteins and results in prolonging the half life of the NF kappaB inhibitors IkappaBalpha and IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Decreased NF kappaB activation in non CD 28 costimulation resulted from the failure to translocate a critical NF kappaB member , c Rel , to the nuclear compartment due to the lack of IkappaBbeta inactivation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| We recently reported that activation of rat mdr1b in cultured cells by 2 AAF involves a cis activating element containing a NF kappaB binding site located 167 to 158 of the rat mdr1b promoter . 2 AAF activates IkappaB kinase ( IKK ) , resulting in degradation of IkappaBbeta and activation of NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Activation of NF kappaB occurs predominantly through the p 75 receptor , and TrkA activity suppresses NF kappaB activation and retards IkappaBbeta degradation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The impaired NF kappaB activation in Bcr Abl expressing cells was not due to absence of the NF kappaB proteins p 65 , p 50 , or p 100 or of IkappaBalpha or IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Exposure of neurons to sAPPalpha resulted in a decrease in the level of IkappaBbeta and an increase in NF kappaB DNA binding activity , both of which were blocked by wortmannin , suggesting that the transcription factor NF kappaB may be a downstream target of the PI ( 3 ) K Akt pathway that may play a role in the cell survival promoting action of sAPPalpha . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Our study reports that infection of cultured astrocytes with TMEV resulted in a time dependent phosphorylation of IkappaBalpha , degradation of IkappaBalpha and IkappaBbeta , activation of NF kappaB and expression of NOS 2 . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| These events were preceded by degradation of inhibitors kappaBalpha ( IkappaBalpha ) and kappaBI ( IkappaBbeta ) , and activation of nuclear factor kappaB ( NF kappaB ) in the lung . ^^^ Degradation of IkappaBalpha and activation of NF kappaB in the lung were not altered by endotoxin tolerance , whereas kinetics of IkappaBbeta degradation was only delayed . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Although the abundance of the inhibitor protein IkappaBbeta was higher in 267B1 / Ki ras cells than in 267B1 cells , an electrophoretic mobility shift assay suggested that IkappaBalpha is responsible for the activation of NF kappaB in the former cells . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Cerulein induces NF kappaB / Rel via activation of IkappaB kinase ( IKK ) , which causes degradation of IkappaBalpha but not IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| IL 10 and IL 13 attenuate NF kappaB activation by interfering with breakdown of IkappaBalpha , while SLPI likewise suppresses NF kappaB activation , but by interfering with breakdown of IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| This is due to the Taxol mediated reactivation of RelA through phosphorylation and degradation of IkappaBbeta and the re expression of NF kappaB regulated bcl xl gene in these cancer cells as ectopic expression of the bcl xl gene confers resistance to Taxol induced apoptosis in PS 341 sensitized cells . ^^^ These results demonstrate the important function of various NF kappaB / IkappaB complexes in regulating anti apoptotic genes in response to apoptotic stimuli , and they raise the possibility that NF kappaB : IkappaBalpha and NF kappaB : IkappaBbeta complexes are regulated by different upstream activators , and that NF kappaB plays a key role in pancreatic tumorigenesis . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The NF kappaB binding proteins IkappaBbeta and IkappaBgamma were prevalent in microsomes , while IkappaBalpha and IkappaB epsilon proteins were absent . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Ikappa Bgamma and prototypical Ikappa Bs use a similar mechanism to bind but a different mechanism to regulate the subcellular localization of NF kappa B . p 105 , also known as NF kappaB 1 , is an atypical IkappaB molecule with a multi domain organization distinct from other prototypical IkappaBs , like IkappaBalpha and IkappaBbeta . ^^^ In the case of IkappaBalpha and IkappaBbeta , the specific NF kappaB subunit in the complex is p 65 . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The model demonstrates that IkappaBalpha is responsible for strong negative feedback that allows for a fast turn off of the NF kappaB response , whereas IkappaBbeta and epsilon function to reduce the system ' s oscillatory potential and stabilize NF kappaB responses during longer stimulations . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NF kappaB activity , as assessed by IkappaBalpha or IkappaBbeta degradation , electrophoretic mobility shift assay and NF kappaB gene reporter assays , is shown to be unaffected by caspase inhibition . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| IkappaBalpha and IkappaBbeta are regulators of the nuclear factor kappaB ( NF kappaB ) transcription factor family . ^^^ We have found that IkappaBbeta expression is localized within the haploid spermatid stages of spermatogenesis and follows the expression of nuclear NF kappaB . ^^^ These results therefore suggest that IkappaBbeta may be a novel target for transcription factors of the HMG box SRY / Sox family and imply a potential role for NF kappaB / IkappaBbeta in spermatogenesis . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Taken together , our data implicate distinct roles for IkappaBalpha and IkappaBbeta in regulating various NF kappaB activities . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NF kappa B p 65 mRNA and 1 kappa B beta mRNA were measured by reverse transcription PCR and in situ hybridization . ^^^ RESULTS : The expressions of NF kappa B p 65 mRNA and 1 kappa B beta mRNA 6 hours after the surgery were 16 % + / 4 % and 31 % + / 9 % respectively ( P < 0 . 01 vs . control vein ) . ^^^ The expression of NF kappa B p 65 mRNA reached in peak during the period 3 days to 7 days after the surgery ( 37 % + / 12 % and 34 % + / 10 % respectively , P < 0 . 01 vs . other teams ) , however , the 1 kappa B beta mRNA expression reached its peak during 1 to 2 weeks after the surgery ( 53 % + / 17 % and 49 % + / 10 % respectively , P < 0 . 01 vs . other teams ) . ^^^ The expressions of NF kappa B p 65 mRNA and 1 kappa B beta mRNA recovered to their baseline values 6 weeks after surgery . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| KappaB Ras binds to the unique insert within the ankyrin repeat domain of IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta 10 NF kappaB complexes . ^^^ The IkappaBalpha and IkappaBbeta proteins inhibit the transcriptional potential of active NF kappaB dimers through stable complex formation . ^^^ It has been shown that inactive IkappaBalpha 10 NF kappaB complexes shuttle in and out of the nucleus , whereas IkappaBbeta 10 NF kappaB complexes are retained exclusively in the cytoplasm of resting cells . ^^^ Deletion of the IkappaBbeta insert renders IkappaBbeta 10 NF kappaB complexes capable of shuttling between the nucleus and cytoplasm , similar to IkappaBalpha 10 NF kappaB complexes . ^^^ A small Ras like G protein , kappaB Ras , participates with the IkappaBbeta insert to effectively mask the NF kappaB nuclear localization potential . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Treatment with PPARgamma agonists restored IkappaBalpha , IkappaBbeta , and HSP 70 levels to values equal or above those observed in control animals , and reduced activation of cortical NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| X ray crystal structure of an IkappaBbeta 10 NF kappaB p 65 homodimer complex . ^^^ We report the crystal structure of a murine IkappaBbeta 10 NF kappaB p 65 homodimer complex . ^^^ One NF kappaB p 65 subunit nuclear localization signal clearly contacts IkappaBbeta , whereas a homologous segment from the second subunit of the homodimer is mostly solvent exposed . ^^^ Primary sequence analysis and differences in the mode of binding at the IkappaBbeta sixth ankyrin repeat and NF kappaB p 65 homodimer suggest a model for nuclear IkappaBbeta . ^^^ These unique structural features of IkappaBbeta may contribute to its ability to mediate persistent NF kappaB activation . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| In this study , inhibitor kappaBalpha ( IkappaBalpha ) and IkappaBbeta protein production was not affected by the chemical protease inhibitors and , more importantly , BCG led to the rapid but transient induction of NF kappaB activity . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The degradation of 1 kappa B beta in the cytosol and the reappearance in the nucleus indicated a persistent NF kappa B activation in celiac disease . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| NF kappa B activity is regulated by its association with the inhibitory 1 kappa B proteins , among which 1 kappa B alpha and 1 kappa B beta are the most abundant . 1 kappa B proteins are widely expressed in different cells and tissues and bind to similar combinations of NF kappa B proteins . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Transient transfection of NF kappaB promoter construct and dominant negative plasmid of IkappaBbeta kinase showed that COX 2 transcription is mediated through p 38 ( MAPK ) and NF kappaB pathways . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| MEKK 3 is involved in the formation of the IkappaBalpha : NF kappaB / IKK complex , whereas MEKK 2 participates in assembling the IkappaBbeta : NF kappaB / IKK complex ; these two distinct complexes regulate the proinflammatory cytokine induced biphasic NF kappaB activation . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| This increase in NF kappaB activation caused by proteasome inhibitors was accompanied by an increase in IkappaB kinase activation and a degradation of IkappaBalpha but not IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Our studies reveal that a commonly used non steroid anti inflammatory drug , acetylsalicylic acid ( aspirin ) prevents H2O2 induced NF kappaB activation in a dose dependent manner through inhibition of phosphorylation and degradation of IkappaBalpha and IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Electrophoretic mobility shift assay ( EMSA ) and Western Blot were used to detect the activation of NF kappaB , nuclear translocation of p 65 subunit and degradation of IkappaBalpha and IkappaBbeta in rat renal tissue . ^^^ Significant up regulation of NF kappaB activation , nuclear translocation of p 65 subunit , and degradation of IkappaBalpha and IkappaBbeta were also observed in NTN rat renal tissue , as compared to the control group . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Foxj 1 suppressed NF kappaB transcription activity in vitro , and Foxj 1 deficient T cells possessed increased NF kappaB activity in vivo , correlating with the ability of Foxj 1 to regulate IkappaB proteins , particularly IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Furthermore , inhibited NF kappaB activation was characterized by reduced degradation , phosphorylation , or both of IkappaBalpha and IkappaBepsilon but not IkappaBbeta and by reduced phosphorylation of Ser 536 , located in the trans activation domain of p 65 . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The initial decline in IkappaBbeta occurred in as little as 30 min post NMDA exposure , coinciding with early NF kappaB activity . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Because NF kappaB translocates to the nucleus upon the phosphorylation and degradation of inhibitor of IkappaB , we also used Western blots to measure cytosolic protein levels of IkappaBalpha and IkappaBbeta , and the IkappaB kinases , IKKalpha and IKKbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Infection with wild type amastigotes resulted in a time dependent proteolytic degradation of IkappaBalpha and IkappaBbeta and the related protein NF kappaB . ^^^ Cysteine peptidase inhibitors prevented IkappaBalpha , IkappaBbeta , and NF kappaB degradation induced by amastigotes , and recombinant CPB2 . 8 , an amastigote specific isoenzyme of CPB , was shown to degrade GST IkappaBalpha in vitro . ^^^ LPS mediated IkappaBalpha and IkappaBbeta degradation was not affected by these inhibitors , confirming that the site of degradation of IkappaBalpha , IkappaBbeta , and NF kappaB by the amastigotes was not receptor driven , proteosomal mediated cleavage . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Some IkappaB proteins , including IkappaBalpha and IkappaBbeta , can regulate transcription by modulating the concentration of active NF kappaB complexes within the nucleus . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical evaluation included members of the NF kappaB ( p 50 , p 65 , p 52 , c Rel , Rel B ) and the IkappaB ( IkappaBalpha , IkappaBbeta , IkappaBepsilon , Bcl 3 ) families , as well as putative targets of NF kappaB such as Flip , Bcl xL , Cyclin D 1 , and oestrogen and progesterone receptors . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Ethyl pyruvate had no effect on the degradation of IkappaBalpha or IkappaBbeta in lipopolysaccharide stimulated RAW 264 . 7 cells , suggesting that ethyl pyruvate acts distally to this step in the activation of NF kappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| PS 341 treatment of these cells stabilized IkappaBalpha , IkappaBbeta , IkappaBvarepsilon , p 21 , p 27 and p 53 proteins and selectively inhibited Rel A DNA binding NF kappaB complexes . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Coexpression of IkappaBalpha , IkappaBbeta , IKKalpha , IKKbeta , NIK and TRAF 2 dominant negative constructs , with LMP 1 inhibited the activation of the CCL 5 promoter by LMP 1 , suggesting that LMP 1 induces CCL 5 via NF kappaB signaling . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| To elucidate the underlying mechanisms , we investigated the expression of NF kappaB subunits , p 65 and p 50 , and IkappaB proteins , IkappaBalpha and IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Through the utilization of immunoprecipitation techniques , protein protein interactions between NF kappaB family members IkappaBalpha , IkappaBbeta , p 50 , and p 65 ( Rel A ) were identified with an Xpress tagged dominant negative c Jun ( TAM 67 ) protein . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| In contrast to the defined regulatory interplay between NF kappaB and IkappaBalpha , much less is known regarding the regulation of IkappaBbeta by NF kappaB . ^^^ Using p 65 ( / ) fibroblasts , we show that IkappaBbeta is profoundly reduced in these cells , but not in other NF kappaB subunit knockouts . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The alteration in NF kappaB DNA binding activity was neither accompanied by any change in the expression of the inhibitor kappaB ( IkappaB ) kinases , IKKalpha , IKKbeta , and IKKgamma nor in the expression of the NF kappaB inhibitor proteins , IkappaBalpha and IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| We demonstrate here that Foxj 1 also inhibits humoral immune responses intrinsically in B cells ; Foxj 1 deficiency in B cells results in spontaneous and accentuated germinal center formation , associated with the development of pathogenic autoantibodies and accentuated responses to immunizations all reflecting excessive activity of NF kappaB and its target gene IL 6 , and correlating with a requirement for Foxj 1 to regulate the inhibitory NF kappaB component IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| This ability was attributed to blockage of the persistent phase of TNF alpha induced NF kappaB activation for the following reasons : ( 1 ) the initial phase of NF kappaB transcriptional activation was not affected by the MC 159 protein ; ( 2 ) the MC 159 protein inhibited TNF alpha directed degradation of IkappaBbeta , but not IkappaBalpha ; and ( 3 ) expression of the late NF kappaB regulated cell genes , TNF alpha and CCL 2 , was decreased in the presence of the MC 159 protein while transcription of the early NF kappaB regulated cell gene , CXCL 1 , was not altered . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| CoMTsAg induced maximal nuclear binding of NF kappaB p 65 and p 50 by 1 h , with IkappaBalpha and IkappaBbeta decay within 15 min . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| We show that in cells depleted of IkappaBalpha , IkappaBbeta and IkappaBepsilon proteins , a small fraction of p 65 binds DNA and leads to constitutive activation of NF kappaB target genes , even without stimulation , whereas most of the p 65 remains cytoplasmic . ^^^ These results indicate that although IkappaBalpha , IkappaBbeta and IkappaBepsilon proteins could be dispensable for cytoplasmic retention of NF kappaB , they are essential for preventing NF kappaB dependent gene expression in the basal state . ^^^ We also show that in the absence of IkappaBalpha , IkappaBbeta and IkappaBepsilon proteins , cytoplasmic retention of NF kappaB by other cellular proteins renders the pathway unresponsive to activation . . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Thus , poly 1 induced an increase in nuclear factor kappaB ( NF kappaB ) transcriptional activity due to a long term degradation of inhibitory NF kappaB ( IkappaB ) beta while lipopolysaccharide ( LPS ) induced the degradation of both IkappaBalpha and IkappaBbeta . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Eight weeks later we measured TBARS and hydroperoxide initiated chemiluminescence ( QL ) in liver as markers of oxidative stress , and activities of the antioxidant enzymes catalase , superoxide dismutase ( SOD ) , and glutathione peroxidase , NF kappaB activation by an electrophoretic mobility shift assay and expression of IkappaB kinases ( IKKalpha and IKKbeta ) , the inhibitor IkappaB ( IkappaBalpha and IkappaBbeta ) , and iNOS by Western blot . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The enhanced activation of NF kappaB by resveratrol progressed for at least 24 h and was accompanied by sustained down regulation of an endogenous NF kappaB inhibitor , IkappaBbeta , but not IkappaBalpha . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Moreover , equol blocked degradation of IkappaBalpha and IkappaBbeta and nuclear translocation of p 65 subunit of NF kappaB in activated RAW 264 . 7 cells . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| Ang 2 also decreased the cytosolic level of the inhibitor of NF kappaB ( IkappaB ) and increased the phosphorylation of IkappaB subunits , IkappaBalpha and IkappaBbeta , as well as the phosphorylation of IkappaB kinase ( IKK ) subunits , IKKalpha and IKKbeta , suggesting that Ang 2 enhanced the breakdown of IkappaB . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Compared with asymptomatic plaques , symptomatic plaques had more macrophages , T lymphocytes , and HLA DR+ cells ( p < 0 . 001 ) ; more ubiquitin proteasome activity and NFkB ( p < 0 . 001 ) ; and more markers of oxidative stress ( nitrotyrosine and O 2 production ) and MMP 9 ( p < 0 . 01 ) along with a lesser collagen content and IkB beta levels ( p < 0 . 001 ) . ^^^ |
|
| Interacting proteins: Q15653 and P19838 |
Pubmed |
SVM Score :0.0 |
| The classical NF kappaB pathway activates the IKK complex that controls the inducible degradation of most IkappaB family members that are IkappaBalpha , IkappaBbeta , IkappaBvarepsilon and p 105 . ^^^ |
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