| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Molecular interactions of the Src homology 2 domain protein Shb with phosphotyrosine residues , tyrosine kinase receptors and Src homology 3 domain proteins . ^^^ The molecular interactions of the Src homology 2 ( SH 2 ) domain and the N terminal proline rich sequence motifs ( pro 1 to pro 5 ) of the SH 2 protein Shb with other components were presently characterised . ^^^ The pro 4 / pro 5 motif of Shb binds in vitro particularly well to the Src , p 85 alpha PI 3 kinase and Eps 8 SH3 domains expressed as GST fusion proteins . ^^^ In vivo association between Shb SH 3 domain proteins 5 Src and Eps 8 was detected by coimmunoprecipitation . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Shb is a ubiquitously expressed Src homology 2 protein . ^^^ A cDNA containing a motif with strong homology to Src homology 2 ( SH 2 ) domains was found using this procedure and called Shb . ^^^ Shb contains the proline rich sequence PPPGPGR between the two proposed initiator methionines which resembles a sequence for binding to Src homology 3 ( SH 3 ) domains . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| To increase our understanding of the role of the Src homology 2 ( SH 2 ) domain containing protein Shb in the mitogenic signal transduction , Shb mRNA contents were determined in the fibroblast like NIH3T3 cells and the insulin producing beta TC 1 cells under various conditions . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Apoptosis of NIH3T3 cells overexpressing the Src homology 2 domain protein Shb . ^^^ To understand the role of the Src homology 2 ( SH 2 ) domain protein Shb in the signal transduction of tyrosine kinase receptor , NIH3T3 cells were transfected with a DNA construct expressing the Shb cDNA ( NIHSHB cells ) . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Modulation of Src homology 3 proteins by the proline rich adaptor protein Shb . ^^^ The expression of the Shb binding proteins Eps 8 , Src , and p 85 PI3 kinase , PI 3 kinase activity , and MAP kinase activation were assessed in wild type NIH3T3 cells and in NIH3T3 cells overexpressing the Shb cDNA . ^^^ The Eps 8 protein content and Eps 8 mRNA steady state levels were downregulated , whereas the protein contents of Src and p 85 PI3 kinase were unaffected by Shb overexpression . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Shb is a recently described Src homology 2 ( SH 2 ) domain containing adaptor protein . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| The Src homology 2 domain protein Shb transmits basic fibroblast growth factor and nerve growth factor dependent differentiation signals in PC 12 cells . ^^^ To assess a possible role for the Src homology 2 ( SH 2 ) domain adaptor protein Shb in PC 12 cell signal transduction and differentiation , we have investigated the expression of Shb in PC 12 cells and found that the differentiation factors nerve growth factor ( NGF ) and basic fibroblast growth factor ( bFGF ) , as well as the PC 12 cell mitogen epidermal growth factor , increased Shb protein expression and Shb mRNA steady state levels . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Requirement of the Src homology 2 domain protein Shb for T cell receptor dependent activation of the interleukin 2 gene nuclear factor for activation of T cells element in Jurkat T cells . ^^^ Overexpression of Src homology 2 domain defective Shb caused diminished phosphorylation of LAT and consequently the activation of mitogen activated protein kinases was decreased upon TCR stimulation . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Induction of apoptosis was dependent on the heparin binding ability of endostatin and the expression of Shb with a functional Src homology 2 ( SH 2 ) domain . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| The Src homology ( SH ) 2 domain adaptor protein Shb has previously been shown to interact with the platelet derived growth factor ( PDGF ) beta receptor . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| NGF Dependent neurite outgrowth in PC 12 cells overexpressing the Src homology 2 domain protein shb requires activation of the Rap 1 pathway . ^^^ The Src homology 2 ( SH 2 ) domain adaptor protein Shb has been shown to transmit NGF and FGF 2 dependent differentiation signals in PC 12 cells . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Shb is a Src homology 2 domain adaptor protein which participates in tyrosine kinase signaling . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of LAT creates binding sites for the Src homology 2 ( SH 2 ) domains of other proteins , including PLC gamma 1 , Grb 2 , Gads , Grap , 3BP2 , and Shb , and indirectly binds SOS , c Cbl , Vav , SLP 76 , and Itk . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Transgenic CBA mice expressing either the tyrosine kinase GTK ( gut tyrosine kinase ) or the adaptor protein SHB ( Src homology 2 protein of beta cells ) under the control of the rat insulin promoter exhibited an increased beta cell mass , but also elevated cytokine induced islet cell death compared with control mice . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Role of the Src homology 2 domain containing protein Shb in murine brain endothelial cell proliferation and differentiation . ^^^ To study the role of the Src homology 2 ( SH 2 ) domain containing protein Shb in angiogenesis , wild type Shb and SH 2 domain mutated Shb ( R522K Shb ) were overexpressed in murine immortalized brain endothelial cells . ^^^ The Shb induced cytoskeletal rearrangements were not dependent on phosphatidylinositol 3 ' kinase activity , but could be reversed by inhibition of Src family kinases . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| The Shb adaptor protein causes Src dependent cell spreading and activation of focal adhesion kinase in murine brain endothelial cells . ^^^ IBE cells overexpressing wild type or R522K Shb ( with an inactive Src homology 2 ( SH 2 ) domain ) displayed increased FAK phosphorylation as well as enhanced spreading when seeded on collagen . ^^^ FGF 2 induced tyrosine phosphorylation of Shb was dependent upon Src activity but independent of FAK activation . ^^^ The use of Rat 1 fibroblasts overexpressing a temperature sensitive 5 Src ( tsLA 29 ) confirmed that active Src enhanced Shb phosphorylation . ^^^ The data indicate that Shb binds directly to FAK and regulates its phosphorylation leading to enhanced cell spreading in a Src dependent manner . . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Overexpression of the Src homology 2 domain protein Shb in beta cells of transgenic mice has been shown to promote an increased beta cell mass . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Recent experiments have unravelled novel signal transduction pathways that involve the SRC homology 2 ( SH 2 ) domain adapter protein SHB . ^^^ Upstream of SHB in some cells lies the SRC like FYN Related Kinase FRK / RAK ( also named BSK / IYK or GTK ) . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrate that Shb is phosphorylated in an Src dependent manner upon vascular endothelial growth factor ( VEGF ) stimulation using porcine aortic endothelial cells expressing the human VEGF receptor 2 ( VEGFR 2 ) ( KDR ) . ^^^ Furthermore , in a glutathione S transferase pull down assay , the Src homology 2 domain of Shb was shown to interact with phosphorylated tyrosine 1175 in the C terminal tail of VEGFR 2 . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| Previous studies suggested a role of the signal transduction protein SRC homology 2 ( SH 2 ) protein of Beta cells ( SHB ) for the development of both pancreatic 3 cells and blood vessels . ^^^ |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15464 and P12931 |
Pubmed |
SVM Score :0.0 |
| NA |
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