| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.7862823 |
| We hypothesized that SAFB 1 functions as a scaffolding protein to recruit proteins such as RFP into proximity with ESR 1 . 0.7862823^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : The predictive accuracy of HET for future ischemic events in the thrombolytic era is markedly reduced . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| SAFB 2 , a new scaffold attachment factor homolog and estrogen receptor corepressor . ^^^ We have characterized previously the nuclear matrix protein / scaffold attachment factor ( SAFB ) as an estrogen receptor corepressor and as a potential tumor suppressor gene in breast cancer . ^^^ As shown previously for SAFB 1 , SAFB 2 functions as an estrogen receptor corepressor , and its overexpression results in inhibition of proliferation . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation assays revealed that estrogen receptor and corepressors were bound to the E cadherin promoter , and that overexpression of corepressors such as scaffold attachment factor B resulted in enhanced repression of E cadherin . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Using the DE domain of human peroxisome proliferator activated receptor gamma ( PPARgamma ) as bait in a yeast two hybrid screen of a human adipose tissue library , we isolated the scaffold attachment factor B 1 ( SAFB1 / HET / HAP ) , which was previously shown to be a corepressor of estrogen receptor alpha . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| We first documented that ER so measured agreed with results by established radioligand based assays [ dextran coated charcoal ( DCC ) and hydroxylapatite ( HAP ) ] for in house breast carcinomas and for proficiency testing specimens . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| To test the possibility that the hap protein might also be a retinoid receptor , a chimaeric receptor was created by replacing the putative DNA binding domain of hap with that of the human oestrogen receptor ( ER ) . ^^^ The resulting hap ER chimaera was then tested for its ability to trans activate an oestrogen responsive reporter gene ( vit tk CAT ) in the presence of possible receptor ligands . ^^^ Here we show that retinoic acid ( RA ) at physiological concentrations is effective in inducing the expression of this reporter gene by the hap ER chimaeric receptor . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| The calf uterine estrogen receptor labeled with [ 3H ] E 2 at 0 C ( state with low affinity for E 2 ) was immobilized on hydroxylapatite ( HAP ) in the absence ( 8S oligomer ) or presence ( 4S monomer ) of 0 . 4 M KCl and heated at 28 C in the presence of unlabeled diethylstilbestrol . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Samples of human breast cancer tissue were analyzed for estrogen receptor content ( ER ) with a hydroxylapatite method ( HAP ) as well as a dextran coated charcoal method ( DCC ) . ^^^ It was also found that the DCC assay underestimated the ER activity in the tumors compared to the HAP assay . ^^^ A significant higher background radioactivity was obtained in the HAP assay , and in some tumors one assay would classify them as ER negative and another as ER positive . . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| When ERC from rats treated with estradiol for 30 min was applied to HAP or DEAE columns , two different ER binding components were seen . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Hydroxylapatite ( HAP ) is known to adsorb ER . ^^^ Scatchard plot analysis of [ 3H ] estradiol binding patterns of HAP batches to which cytosolic ER had been adsorbed revealed that AB and / or C domains are mainly responsible for this property . ^^^ This did not occur with ER preparations devoid of exposed ABC domains obtained by selective immunoextraction with H 226 anti ER monoclonal antibody prior to HAP assay . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| The phosphocalcic nature of the HAP matrix may explain this phenomenon which was observed with cytosolic ER from various origins . . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Two vectors were introduced into the genome of recipient ES cells , successively : ( 1 ) a bicistronic gene trap vector encoding the beta galactosidase / neo ( R ) fusion protein and the Cre ER ( T 2 ) ( Cre recombinase fused to a mutated ligand binding domain of the human estrogen receptor ) and ( 2 ) a bicistronic gene trap vector encoding the hygro ( R ) protein and the human alkaline phosphatase ( hAP ) , the expression of which is prevented by tandemly repeated stop of transcription sequences flanked by loxP sites . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| The ESR signal intensities at near g = 2 in DAp , c DAp , and stoichiometric hydroxyapatite ( HAp ) after 10 ray irradiation , were proportional to the absorbed dose in the range from 6 to 380 Gy . ^^^ These ESR signal intensities decreased when the 10 ray irradiated DAp , c DAp , and HAp was suspended in the simulated body fluid . ^^^ This fact suggests that the surface layer contained high density of ESR active species in DAp , c DAp , and HAp dissolved in the simulated body fluid . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| The novel endoplasmic reticulum ( ER ) targeted protein HAP induces cell apoptosis by the depletion of the ER Ca ( 2+ ) stores . ^^^ HAP , a novel human apoptosis inducing protein , was identified to localize exclusively to the endoplasmic reticulum ( ER ) in our previous work . ^^^ In the present work , we reported that ectopic overexpression of HAP proteins caused the rapid and sustained elevation of the intracellular cytosolic Ca ( 2+ ) , which originated from the reversible ER Ca ( 2+ ) stores release and the extracellular Ca ( 2+ ) influx . ^^^ The HeLa cells apoptosis induced by HAP proteins was not prevented by establishing the clamped cytosolic Ca ( 2+ ) condition , or by buffering of the extracellular Ca ( 2+ ) with EGTA , suggesting that the depletion of ER Ca ( 2+ ) stores rather than the elevation of cytosolic Ca ( 2+ ) or the extracellular Ca ( 2+ ) entry contributed to HAP induced HeLa cells apoptosis . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| We measured interleukin 6 ( IL 6 ) , C reactive protein ( CRP ) , alpha 1 antitrypsin ( a1AT ) , acid alpha 1 glycoprotein ( a1AG ) , haptoglobin ( HAP ) , transferrin ( TRF ) , hemoglobin ( Hb ) , beta 2 microglobulin ( beta2M ) and erythrocyte sedimentation rate ( ESR ) in 42 newly diagnosed multiple myeloma patients and 25 normal controls . ^^^ Mean + / SD values of IL 6 , CRP , a1AT , a1AG , HAP , beta2M , and ESR were significantly higher and Hb significantly lower than those found in the controls . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| In attempting to produce the HAP , endoplasmic reticulum ( ER ) targeted apoptosis inducing protein , as a GST fusion protein we found that the expression of HAP , but not GST alone , induced bacterial cell death . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Adsorption of Gd3+ and Mn2+ into hydroxyapatite ( HAP ( Ca 5 ( PO 4 ) 3 ( OH ) 2 ) was studied by ESR , ion chromatography and ICP AES to investigate the U uptake model . ^^^ The ESR signals of Gd3+ and Mn2+ were observed overlapping the CO 2 signals , however , the dose response curves of the CO 2 signal were similar to that of control HAP . . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| ER Ca2+ depletion triggers apoptotic signals for endoplasmic reticulum ( ER ) overload response induced by overexpressed reticulon 3 ( RTN3 / HAP ) . ^^^ Our previous study demonstrated that the overexpression of reticulon 3 ( RTN 3 , also named HAP , homologue of ASY protein ) caused apoptosis with the depletion of ER Ca ( 2+ ) stores . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Previous experiments have suggested that het erodimer formation in the ER arises from interaction between the luminal , NH 2 terminal domains of the subunits . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Gel shift analysis revealed the binding of a protein ( termed HET for Hsp 27 ERE TATA binding protein ) to this region that was neither the estrogen receptor nor TATA binding protein . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Tamoxifen bound estrogen receptor ( ER ) strongly interacts with the nuclear matrix protein HET / SAF B , a novel inhibitor of ER mediated transactivation . ^^^ Here we report that the nuclear matrix protein / scaffold attachment factor HET / SAF B is an ER interacting protein . ^^^ ER and HET / SAF B interact in in vitro binding assays , with HET binding to both the ER DNA binding domain and the hinge region . ^^^ Coimmunoprecipitation experiments reveal that HET / SAF B and ER associate in cell lines in the presence or absence of estradiol , but binding is increased by the antiestrogen tamoxifen . ^^^ HET / SAF B enhances tamoxifen antagonism of estrogen induced ER mediated transactivation , but at high concentrations can inhibit both estrogen and tamoxifen induced ER activity . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Ovariectomized ( OVX ) estrogen receptor alpha knockout ( ERalphaKO ) mice displayed impaired performance on the IA task and OVX heterozygotic ( HET ) mice exhibited performance that was intermediate between ERalphaKO and wild type ( WT ) mice . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| The ER glucosidase inhibitor , castanospermine , decreased secretion of both hLPL and hHL from het cells by approximately 70 % , but by only approximately 45 % from cld cells . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Patients involved in a high energy trauma ( HET ) are usually admitted for clinical observation , even when no significant injury is found after standard care in the emergency room ( ER ) . ^^^ Patients after a HET with two minor injuries or less , diagnosed during the standard ER care , were included . ^^^ CONCLUSION : There is no evidence for the necessity of clinical observation of HET patients with minimal or no injuries diagnosed after standard ER stabilization and evaluation . . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| We have previously shown that SAFB 1 can repress estrogen receptor ( ERalpha ) mediated transactivation . ^^^ Removal of the RD from SAFB 1 resulted in a dominant negative SAFB 1 protein that increased ligand dependent and independent ERalpha activity . ^^^ We propose a model in which SAFB 1 represses ERalpha activity via indirect association with histone deacetylation and interaction with the basal transcription machinery . . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Immortalized SAFB 1 / mouse embryonic fibroblasts showed cell intrinsic defects including increased transcriptional estrogen receptor alpha activity and enhanced responsiveness to IGF 1 . ^^^ |
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| Interacting proteins: Q15424 and P03372 |
Pubmed |
SVM Score :0.0 |
| Scaffold attachment factor SAFB 1 suppresses estrogen receptor alpha mediated transcription in part via interaction with nuclear receptor corepressor . ^^^ |
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