| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitations revealed that five components of the complex are the splicing associated factors SRm 160 , DEK and RNPS 1 , the mRNA associated shuttling protein Y 14 and the mRNA export factor REF . ^^^ |
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| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| When assembled in vitro , this so called ' exon exon junction complex ' ( EJC ) contains at least five proteins : SRm 160 , DEK , RNPS 1 , Y 14 and REF . ^^^ |
|
| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| These proteins , including Y 14 , Aly / REF , RNPS 1 , SRm 160 , and DEK , are assembled into a stable complex near exon exon junctions of spliced mRNAs . ^^^ |
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| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| Consistent with this evidence , we demonstrate that RNPS 1 , Y 14 , SRm 160 , REF / Aly , TAP , Upf3X and Upf 2 are detected in the nuclear fraction on CBP 80 bound but not eIF4E bound mRNA . ^^^ |
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| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| Mass spectrometry and Western blotting of purified H , C , and mRNP complexes revealed that at least one EJC component , REF / Aly , can interact with pre mRNA prior to spliceosome assembly , whereas Y 14 , Magoh , RNPS 1 , UAP 56 , and SRm 160 are found in intermediate containing spliceosomes . ^^^ Upon exon ligation , association of RNPS 1 , UAP 56 , and SRm 160 is destabilized . ^^^ |
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| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| The proteins of the EJC , Y 14 , Magoh , Aly / REF , RNPS 1 , Srm 160 , and Upf 3 , play critical roles in postsplicing processing , including nuclear export and cytoplasmic localization of the mRNA , and the nonsense mediated mRNA decay ( NMD ) surveillance process . ^^^ |
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| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| The EJC components RNPS 1 , REF , UAP 56 , and Y 14 interact with SRm 160 . ^^^ However , when these factors were tethered to transcripts , only SRm 160 and RNPS 1 stimulated 3 ' end cleavage . ^^^ Whereas SRm 160 stimulated cleavage to a similar extent in the presence or absence of an active intron , stimulation of 3 ' end cleavage by tethered RNPS 1 is dependent on an active intron . ^^^ These results suggest that SRm 160 stimulates cleavage independently of its association with EJC components and that the cleavage stimulatory activity of RNPS 1 may be an indirect consequence of its ability to stimulate splicing . ^^^ |
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| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| Conversely , RNA tethering of the EJC components SRm 160 or RNPS 1 boosts the expression of intronless mRNAs but not of spliced mRNAs . ^^^ |
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| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| Three of the EJC components , Aly / REF , RNPS 1 , and SRm 160 , are found on pre mRNA by the time the spliceosome is formed , whereas Upf3b associates with splicing intermediates during or immediately after the first catalytic step of the splicing reaction ( cleavage of exon 1 and intron lariat formation ) . ^^^ |
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| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| A second EJC protein , RNPS 1 , also has an ATP dependent mobility , but SRm 300 , a protein that binds to SRm 160 and participates with it in RNA splicing , remains immobile after ATP supplementation . ^^^ |
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| Interacting proteins: Q8IYB3 and Q15287 |
Pubmed |
SVM Score :0.0 |
| Using in situ hybridization and confocal microscopy , we observe accumulation of EJC proteins ( REF / Aly , Y 14 , SRm 160 , UAP 56 , RNPS 1 , and Magoh ) and core spliceosome components ( U snRNPs ) at sites of transcription . ^^^ |
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