| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :1.1804646 |
| Akt interacts with these phospholipids , causing its translocation to the inner membrane , where it is phosphorylated and activated by PDK 1 and PDK 2 . 1.1804646^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| The serine threonine kinase Akt is a downstream target of phosphoinositide 3 kinase ( PI 3 kinase ) ; it is activated by the phosphoinositide 3 phosphate dependent kinases PDK 1 and PDK 2 . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| The PtdIns ( 3 , 4 , 5 ) P 3 dependent activation of protein kinase B ( PKB ) by 3 phosphoinositide dependent protein kinases 1 and 2 ( PDK 1 and PDK 2 respectively ) is a key event in mediating the effects of signals that activate PtdIns 3 kinase . ^^^ The catalytic domain of serum and glucocorticoid regulated protein kinase ( SGK ) is 54 % identical with that of PKB and , although lacking the PtdIns ( 3 , 4 , 5 ) P 3 binding pleckstrin homology domain , SGK retains the residues that are phosphorylated by PDK 1 and PDK 2 , which are Thr 256 and Ser 422 in SGK . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| Akt / protein kinase B is regulated by autophosphorylation at the hypothetical PDK 2 site . ^^^ Akt / protein kinase B is regulated by autophosphorylation at the hypothetical PDK 2 site . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| Here we report that the major PKB subtype in platelets is PKBalpha , which is activated by phosphorylation of Thr ( 308 ) and Ser ( 473 ) and has a constitutively phosphorylated Thr ( 450 ) that does not contribute to PKB activation . alpha Thrombin and thrombopoietin activate PKBalpha via PI 3K and trigger the concurrent phosphorylation of Thr ( 308 ) ( via PDK 1 ) and Ser ( 473 ) ( via a not yet identified PDK 2 ) . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| Integrin linked kinase ( ILK ) is an integrin interacting protein kinase which has been identified as a potential PDK 2 , as it is capable of phosphorylating PKB / Akt on Ser 473 , and stimulating its activity . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| Akt activation requires phosphorylation of Thr ( 308 ) and Ser ( 473 ) by 3 phosphoinositide dependent kinase 1 and 2 ( PDK 1 and PDK 2 ) , respectively . ^^^ We conclude that Akt exists in a signaling complex containing p 38 kinase , MK 2 , and Hsp 27 and that p 38 dependent MK 2 activation functions as PDK 2 in human neutrophils . . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| We conclude that , at physiological concentrations , TNF alpha activates endogenous PKB by stimulating PDK 2 ( increase in Ser ( 473 ) phosphorylation ) in a PI 3 kinase dependent ( wortmannin sensitive ) manner , without causing detectable stimulation of PDK 1 ( no increase in Thr ( 308 ) phosphorylation ) or ARNO translocation . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| PDK 2 : a complex tail in one Akt . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| Because both PKCzeta and PKB have been proposed to be required for mediating a number of crucial insulin responses , formation of an active signaling complex containing PKCzeta , PKB , and PDK 2 is an attractive mechanism for ensuring that all the critical sites on targets such as glycogen synthase kinase 3 are phosphorylated . . ^^^ Characterization of PDK 2 activity against protein kinase B gamma . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| PDK 1 , an upstream effector of Akt , was also down regulated following CEES exposure , but two other upstream effectors of Akt , PI 3 K and PDK 2 , remained unchanged . 4 . ^^^ The phosphorylation of Akt at Ser ( 473 ) and Thr ( 308 ) was significantly decreased following CEES treatment , reflecting the suppressed kinase activity of both PDK 1 and PDK 2 . 5 . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| Utilizing the advantages provided by a cell free methodology , we characterized phosphoinositide dependent kinase 2 ( PDK 2 ) , the putative kinase responsible for phosphorylating Akt on Ser 473 . ^^^ Immunodepleting cytosolic PDK 1 from an in vitro reaction containing plasma membrane and cytosol markedly inhibited insulin stimulated phosphorylation of Akt at the PDK 1 site ( Thr 308 ) but had no effect on phosphorylation at the PDK 2 site ( Ser 473 ) . ^^^ Our data indicate that this PDK 2 activity is the result of a kinase distinct from PDK 1 and is not due to autophosphorylation or transphosphorylation of Akt . . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| Membrane bound Akt is then phosphorylated at two sites for its full activation ; Thr 308 in the activation loop of the kinase domain is phosphorylated by 3 phosphoinositide dependent kinase 1 ( PDK 1 ) and Ser 473 in the C terminal hydrophobic motif by a putative kinase PDK 2 . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| PI ( 3 , 4 , 5 ) P 3 is a second messenger essential for the translocation of Akt to the plasma membrane where it is phosphorylated and activated by phosphoinositide dependent kinase ( PDK ) 1 and PDK 2 . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| They further support that PDK 2 is a molecule distinct from PDK 1 and Akt , and that PDK 2 activity is not sufficient for the full activation of Akt in the absence of PDK 1 activity . . ^^^ |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15119 and P31749 |
Pubmed |
SVM Score :0.0 |
| NA |
|