| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.69394325 |
| The tight association between p40phox and p67phox was not affected by treatment with a mixture of deoxycholate and Nonidet P 40 , until subjected to SDS PAGE . 0.69394325^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.51446001 |
| The primary association of p40phox appears to be with p67phox , and it is present in reduced amounts in patients with CGD lacking p67phox . . 0.51446001^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.70303462 |
| Interactions between cytosolic components of the NADPH oxidase : p40phox interacts with both p67phox and p47phox . 0.70303462^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.53632995 |
| In this study , we raised separate antibodies against both the COOH and NH 2 terminal polypeptides of p40phox as well as against the COOH terminal polypeptide of p67phox to examine the mode of interaction between p40phox and p67phox in a complex . 0.53632995^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :1.0022881 |
| We investigated binding interactions between p40phox , p67phox , and Rac and found that Rac 1 GTP displaced p67phox bound to p40phox . 1.0022881^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :1.1380026 |
| P40phox associates with the neutrophil Triton 10 100 insoluble cytoskeletal fraction and PMA activated membrane skeleton : a comparative study with P67phox and P47phox . 1.1380026^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.53642924 |
| However , the interaction between p67phox and p40phox was not affected by either Galphai form . 0.53642924^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The enzyme becomes active after assembly on a membrane bound flavocytochrome b of cytosolic factors p 47 phox , p 67 phox and p 40 phox and of low molecular mass GTP binding proteins . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Examination of 45 non synonymous polymorphisms in ten genes : p47phox ( NCF 1 ) , p67phox ( NCF 2 ) , p40phox ( NCF 4 ) , p22phox ( CYBA ) , gp91phox ( CYBB ) , DUOX 1 , DUOX 2 , TLR 2 , TLR 9 and alpha 1 antitrypsin ( AAT ) in a cohort of 95 lung disease individuals and 95 control individuals did not show an association of these polymorphisms with increased susceptibility to lung disease . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Rac 1 also activates superoxide production by the components ( cytochrome b , p40phox , p67phox , and p47phox ) of the neutrophil oxidase . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Because it was found that the cytosolic oxidase components p67phox , p47phox , and p40phox reside in a complex in resting neutrophils , we studied the role of SH 3 domains in their interaction by use of an overlay technique . ^^^ A specific association of native p67phox to blotted p47phox and blotted p40phox was found . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Mapping the domains of interaction of p40phox with both p47phox and p67phox of the neutrophil oxidase complex using the two hybrid system . ^^^ The superoxide generating NADPH oxidase complex in phagocytic cells is constituted of a heterodimeric flavocytochrome b and cytosolic factors , p67phox , p47phox and p40phox as well as a small G protein Rac ( for review , see Refs . 1 3 ) . ^^^ This truncated form of p40phox consisting of the Src Homology 3 ( SH 3 ) domain to the 3 ' stop codon was also shown to interact with p67phox in the same system . ^^^ Results show that the SH 3 domain of p40phox is sufficient for interaction with p47phox , whereas the C terminus of p40phox but not its SH 3 domain is involved in the interaction with p67phox . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The phagocyte NADPH oxidase is activated during phagocytosis to produce superoxide , following assembly of a membrane integrated cytochrome b 558 with cytosolic proteins , p47phox , p67phox and p40phox , each containing Src homology 3 ( SH 3 ) domains . ^^^ While both p47phox and p67phox are indispensable for the oxidase activity , role of p40phox remains obscure . ^^^ This proline rich region is also the target for binding of p67phox , and the SH 3 domain of p40phox can inhibit the binding of the C terminal one of p67phox to p47phox . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Mechanisms of NADPH oxidase activation : translocation of p40phox , Rac 1 and Rac 2 from the cytosol to the membranes in human neutrophils lacking p47phox or p67phox . ^^^ On neutrophil stimulation , the cytosolic components of NADPH oxidase , p67phox , p47phox , p40phox , as well as the Ras related G proteins Rac 1 and Rac 2 , are translocated from the cytosol to cell membranes where they associate with a flavocytochrome b , forming a functional complex responsible for the production of oxygen radicals in phagocytes . ^^^ In this paper we show that ( a ) in neutrophils from a patient with a form of chronic granulomatous disease ( CGD ) in which p67phox is absent , p47phox and Rac 2 , but not p40phox and Rac 1 were translocated from the cytosol to the membrane on stimulation with formylmethionyl leucylphenylalanine ( fMLP ) or phorbol 12 myristate 13 acetate ( PMA ) ; ( b ) in neutrophils from a patient with a form of CGD in which p47phox is absent , p67phox , p40phox and Rac 1 failed to associate with the membrane on stimulation with fMLP or PMA , whereas Rac 2 was translocated as in normal neutrophils . ^^^ We also show that in neutrophils from a patient lacking p67phox , the amount of cytosolic p40phox was decreased by about 40 % . ^^^ These findings indicate that , on neutrophil stimulation , p67phox mediates the translocation of p40phox and Rac 1 from the cytosol to cell membranes and that Rac 2 associates with the membranes independently of p47phox and p67phox . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Pinpointing the sites of interaction between p47phoz , p67phox and p40phox using the two hybrid system . ^^^ Various constructs of p47phox , p67phox and p40phox cDNAs coding for functional domains were inserted into two hybrid system vectors , expressing fusion proteins either with the DNA binding protein Lex A or with the activation domain of Gal 4 . ^^^ The site of interation of p67phox with p40phox was found to be in the 150 amino acid stretch between the two SH 3 domains of p67phox . ^^^ As the C terminal tail of p40phox which interacts with p67phox contains neither a SH 3 domain nor a polyproline consensus site , it is concluded that a novel type of interaction occurs between p40phox and p67phox . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Genomic structure , chromosomal localization , start of transcription , and tissue expression of the human p 40 phox , a new component of the nicotinamide adenine dinucleotide phosphate oxidase complex . p 40 phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase that copurifies with p 67 phox . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| A third NADPH oxidase subunit , p67phox , is shown to contain four tetratricopeptide repeats ( TPRs ) within its N terminal RaclGTP binding region , and a 28 residue motif in p40phox is demonstrated to be present in protein kinase C isoforms iota / lambda and zeta , and in three ZZ domain containing proteins . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Recently , we have found a novel cytosolic component , p 40 phox , which is tightly associated with p 67 phox . ^^^ The translocation pattern of p 40 phox was similar to those of p 47 phox and p 67 phox . ^^^ However , immunoprecipitation assay revealed that p 40 phox was dissociated from p 47 phox and p 67 phox during activation . ^^^ Interestingly , a synthetic peptide corresponding to carboxyl terminus of p 40 phox inhibited the activation of NADPH oxidase and translocation of p 40 phox , p 47 phox , and p 67 phox , suggesting that p 40 phox might play a role in the activation of NADPH oxidase . ^^^ These observations suggest that p 40 phox is dissociated from p 67 phox during activation , and translocates to the membrane by GTP gamma s independent mechanism . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The human NADPH oxidase is a very complex enzyme , in two ways : 1 . it exists of at least 6 components : cytochrome b 558 ( a heterodimer comprised of gp 91 phox and p 22 phox ) , p 47 phox , p 67 phox , p 40 phox , rac and Rap1A , and 2 . there are multiple signal transduction pathways leading to activation of the NADPH oxidase . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The Prorich motif in p47phox interacts with both p40phox and the COOH terminal SH 3 domain of p67phox . ^^^ CONCLUSIONS : In the resting cell , the Pro rich motif of p47phox interacts with the SH 3 domain of p40phox , which in turn associates with p67phox . ^^^ Upon activation , the p 47 p40phox regulatory complex dissociates , permitting the association of p47phox with the COOH terminal SH 3 domain of p67phox . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Reverse transcriptase polymerase chain reaction ( RT PCR ) using oligonucleotides designed against the known sequences for the human phagocyte NADPH oxidase showed the expression of p 22 phox , p 40 phox , and p 47 phox mRNA , while Western blot analysis of chondrocyte extracts using polyclonal antisera raised against the human phagocyte NADPH oxidase suggested the presence of the p 67 phox polypeptide . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Membrane bound cytochrome b 558 , cytosolic p 47 phox , p 67 phox , and p 40 phox were present in both neutrophils and eosinophils , but the amounts of these components were 1 . 5 to 3 . 3 fold greater in eosinophils than neutrophils . ^^^ Upon activation , p 47 phox , p 67 phox , and p 40 phox were translocated to the membranes in both leukocytes , but larger amounts were translocated in eosinophils than in neutrophils . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Binding studies revealed that coronin attaches to the C terminal half of p40phox , a binding partner of p67phox . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The PC motif : a novel and evolutionarily conserved sequence involved in interaction between p40phox and p67phox , SH 3 domain containing cytosolic factors of the phagocyte NADPH oxidase . ^^^ Among the latter are the three proteins containing Src homology 3 ( SH 3 ) domains , p67phox , p47phox and p40phox . ^^^ While the first two factors are indispensable for the activity , p40phox is tightly associated with p67phox in resting cells and is suggested to have some modulatory role . ^^^ Here we describe a systematic analysis of the interaction between p40phox and p67phox using the yeast two hybrid system and in vitro binding assays with recombinant proteins . ^^^ Both methods unequivocally showed that the minimum requirements for stable interaction are the C terminal region of p40phox and the region between the two SH 3 domains of p67phox . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| It is composed of several subunits , two of which , gp91phox and p22phox , form the membrane bound cytochrome b 558 , while its three cytosolic components , p47phox , p67phox and p40phox , have to translocate to the membrane upon activation . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Taken together , these findings indicate that 1 ) a low expression of some NADPH oxidase components may represent the molecular basis of the low production of O 2 in B lymphocytes ; 2 ) the cytosolic components of NADPH oxidase can not bind to p22phox on the membranes in the absence of gp91phox ; 3 ) p47phox can translocate to the membranes independently of p67phox and p40phox ; and 4 ) gp91phox may have a role in mediating and / or stabilizing the binding of p67phox and p40phox to the membranes of activated cells . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The superoxide generating NADPH oxidase complex of phagocytic cells is a multicomponent system containing a membrane bound flavocytochrome b and a small G protein Rac as well as cytosolic factors p67phox , p47phox and p40phox which translocate to the membrane upon activation . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Another SH 3 domain containing protein p40phox , which is constitutively associated with p67phox in phagocytes , also enters the complex upon cell stimulation . ^^^ Here we describe how we cloned mouse cDNAs encoding p40phox and its partner in phagocytes , p67phox . ^^^ Both p40phox and p67phox comprise several protein binding modules that are structurally and functionally well conserved between mouse and human , indicating their nature as adaptor proteins . ^^^ We have also systematically investigated expression of the gene for p40phox in comparison with those for p67phox and p47phox . ^^^ These observations raise the possibility that the adaptor protein p40phox plays a heretofore unsuspected role via interacting with other proteins in the cells that do not express p67phox or p47phox . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| We also show that the two NADPH oxidase activating factors , p47phox and p67phox are substrates for DNA PK in vitro and that they are present together with p40phox in the nucleus of B cells . ^^^ These results may help solve the paradox that the phox protein triad , p40phox , p47phox and p67phox , is expressed equally in B lymphocytes and neutrophils , whereas the redox component of the NADPH oxidase , a flavocytochrome b , which is well expressed in neutrophils , is barely detectable in B lymphocytes . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The role of magnesium ions in the activation of NADPH oxidase has been investigated using flavocytochrome b 245 and either neutrophil cytosol or mixtures of recombinant p40phox , p47phox , p67phox and Rac 2 . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| It is a multicomponent enzyme comprising a membrane bound flavocytochrome ( cytochrome b 558 ) and at least four cytosolic components : p47PHOX , p67PHOX , p40PHOX , and Rac , a small GTPase . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Interestingly , the variant protein lacked PC ( Phox and Cdc24p ) motif of p40phox , which is assumed to be important for the interaction with p67phox . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| It consists of membranous cytochrome b 558 composed of gp 91 phox and p 22 phox , and four cytosolic components , p 47 phox , p 67 phox , rac p 21 and p 40 phox , which translocate to the membrane upon activation . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase , a superoxide producing enzyme in phagocytic cells , consists of membrane associated cytochrome b 558 and cytosolic components ( p 47 phox , p 67 phox , p 40 phox , rac 1 / 2 ) . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Reconstitution of oxidase activity was performed in vitro by using a heterologous cell free assay consisting of membrane suspended or solubilized and purified cytochrome b 558 that was associated with cytosol or with the isolated cytosolic activating factors ( p 67 phox , p 47 phox , p 40 phox ) from healthy or CGD patients . ^^^ In p 67 phox deficient CGD patients , two cytosolic factors are deficient or missing : p 67 phox and p 40 phox . ^^^ Moreover , the simultaneous addition of recombinant p 67 phox and recombinant p 40 phox reversed the previous complementation in a p 40 phox dose dependent process . ^^^ In vitro , the transition from the resting to the activated state of oxidase , which results from assembling , requires the dissociation of p 40 phox from p 67 phox for efficient oxidase activity . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| It consists of membranous cytochrom b 558 composed of gp 91 phox and p 22 phox , and four cytosolic components , p 47 phox , p 67 phox , rac p 21 and p 40 phox , which translocate to the membrane upon activation . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase activity depends on the assembly of the cytosolic activating factors , p 67 phox , p 47 phox , p 40 phox , and Rac with cytochrome b ( 558 ) . ^^^ In the processes that were studied , p 67 phox displayed a critical function ; it was shown to be involved in both assembly and activation of oxidase complex while p 47 phox proceeded as a positive effector and increased the affinity of p 67 phox with cytochrome b ( 558 ) , and p 40 phox stabilizes the resting state . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Superoxide generating NADPH oxidase consists of the membrane bound cytochrome b 558 ( gp91phox and p22Phox ) and the cytosolic components ( p67phox , p47phox , p40phox and rac ) . ^^^ RESULTS : Northern blot and Western blot analyses revealed that mRNAs and proteins for gp91phox , p67phox and p47phox were expressed after eosinophilic myelocyte stages , whereas mRNAs and proteins for p40phox and rac 2 were expressed from the promyelocyte stage . ^^^ Consistent with the results of Western blotting , immunocytochemical staining of butyrate induced HL 60 clone 15 cells indicated that gp91phox , p22phox , p67phox and p47phox were detected after eosinophilic myelocyte stages ( eosinophilic myelocytes , eosinophilic metamyelocytes , eosinophilic band cells and eosinophilic segmented cells ) , whereas p40phox and rac 2 were expressed from the promyelocyte stage . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| As with the neutrophil NADPH oxidase , the B lymphocyte NADPH oxidase consists of a membrane bound flavocytochrome b and regulatory factors including Rac and the cytosolic phox protein triad p67phox , p47phox , and p40phox . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| A number of cytosolic proteins are involved in its activation / deactivation : p47phox , p67phox , p40phox and the small GTP binding protein , rac . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Treatment with atorvastatin reduced vascular mRNA expression of p22phox and nox 1 and increased aortic catalase expression . mRNA expression of superoxide dismutases , glutathione peroxidase , and NAD ( P ) H oxidase subunits gp91phox , p40phox , p47phox , and p67phox remained unchanged . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Activation of the phagocyte NADPH oxidase complex requires assembly of the cytosolic factors p47PHOX , p67PHOX , p40PHOX , and Rac with the membrane bound cytochrome b 558 . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| We investigated whether VSMCs from human resistance arteries ( HVSMCs ) possess a functionally active , angiotensin 2 ( Ang 2 ) regulated NAD ( P ) H oxidase that contains neutrophil oxidase subunits , including p22phox , gp91phox , p40phox , p47phox , and p67phox . mRNA expression of gp91phox homologues , nox 1 and nox 4 , was also assessed in HVSMCs , human aortic smooth muscle cells , and rat VSMCs . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| This deficiency may have resulted from a gene mutation and / or abnormality of the NADH oxidase components , particularly cytochrome b 558 ( gp91phox and p22phox ) in membrane and cytosol factors p47phox , p67phox , p40phox and others in neutrophil . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The other components of the respiratory burst oxidase are water soluble proteins of cytosolic origin , namely p67phox , p47phox , p40phox and Rac . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The NADPH oxidase complex of phagocytes comprises a membrane associated flavocytochrome b 559 , and 4 cytosolic components : p47phox , p67phox , p40phox , and the small GTPase Rac . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Specifically , GT inhibits PMA stimulated events : p47phox phosphorylation , its incorporation into the cytoskeleton , and the membrane translocation of p67phox , p47phox , and p40phox , which are crucial steps in the assembly of the active NADPH oxidase . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase consists of a membrane heterodimeric protein , composed of gp91phox and p22phox , and cytosolic proteins , p40phox , p47phox and p67phox . ^^^ The sequences of these cDNAs showed that the pufferfish gp91phox , p22phox , p40phox , p47phox and p67phox clones contained open reading frames encoding 565 , 186 , 348 , 423 and 495 amino acids , respectively . ^^^ Comparison of the deduced amino acid sequences showed that the pufferfish gp91phox , p22phox , p40phox , p47phox and p67phox sequences shared 68 . 0 , 61 . 8 , 53 . 8 , 54 . 7 and 41 . 9 % identity with those of human components , respectively . gp91phox has three potential N linked glycosylation sites . gp91phox and p22phox have six and three hydrophobic regions , respectively , that are predicted to be transmembrane regions . p47phox and p67phox have two potential Src homology 3 domains and p40phox has one . ^^^ The level of gp91phox , p47phox and p67phox expression were high only in the blood and kidney , while p22phox and p40phox were constitutively expressed in a wide range of tissues . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Upon cell stimulation , the redox component , membrane bound flavocytochrome b 558 , is activated when the cytosolic factors ( p47phox , p67phox and p40phox , as well as the small GTPase Rac ) associate with it after translocating to the membrane . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The PB 1 ( Phox and Bem 1 ) domain is a recently identified module that mediates formation of a heterodimeric complex with other PB 1 domain , e . g . the complexes between the phagocyte oxidase activators p67phox and p40phox and between the yeast polarity proteins Bem1p and Cdc24p . ^^^ These PB 1 domains harbor either a conserved lysine residue on one side or an acidic OPCA ( OPR / PC / AID ) motif around the other side ; the lysine of p67phox or Bem1p likely binds to the OPCA of p40phox or Cdc24p , respectively , via electrostatic interactions . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase complex is composed of membrane proteins , p22PHOX and gp91PHOX , and cytosolic proteins , p40PHOX , p47PHOX and p67PHOX . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Immunological assays have demonstrated the presence of p67phox and p47phox in lens tissue , while PCR and RT PCR reactions amplified DNA products corresponding to the p67phox , p40phox , p22phox , gp91phox , and Rac 1 components of the NADPH oxidase complex from human and mouse lens cDNA libraries . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| During activation of the cell cytosolic phox proteins ( p47phox , p67phox , p40phox , and Rac 2 ) translocate to the plasma membrane and specific granules fuse with the plasma membrane increasing the amount of flavocytochrome b ( 558 ) . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis showed that both cell lines expressed the phagocyte oxidase ( phox ) cytosolic proteins Rac 1 , p40phox , and p67phox . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| In addition , RT PCR revealed that SH SY5Y cells expressed mRNA of gp91phox , p22phox and cytosolic p47phox , p67phox and p40phox , the components of the plasma membrane NADPH oxidase . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| This system is initiated by the translocation of cytosolic proteins p67phox , p47phox and p40phox to be in close contact with membrane flavocytochrome b 558 . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Structural comparison of the PKCiota and Par6alpha PB 1 complex with the p40phox and p67phox PB 1 domain complex , subunits of neutrophil NADPH oxidase , reveals that the specific interaction is achieved by tilting the interface so that the insertion or extension in the sequence is engaged in the specificity determinant . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Effects of p47phox C terminus phosphorylations on binding interactions with p40phox and p67phox . ^^^ Structural and functional comparison of p40phox and p67phox SH 3 domains . ^^^ These serines surround a polyproline motif that can interact with the Src homology 3 ( SH 3 ) module of p40phox ( SH3p40 ) or the C terminal SH 3 of p67phox ( C SH3p67 ) . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Quantitative analysis of cytosolic components showed moderately reduced amount of p 40 phox , p 47 phox , and p 67 phox in neutrophils from cord blood . ^^^ CONCLUSION : The normal to increased respiratory burst of intact cord blood neutrophils is the result of alterations to oxidase components : increased content of cytochrome b ( 558 ) in the plasma membrane and decreased levels of cytosolic components p 47 phox , p 67 phox , and p 40 phox . . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase is multi component enzyme , which comprises the cytosolic proteins p40Phox , p47Phox , and p67Phox and the two membrane proteins , gp91Phox and p22Phox , and which is well characterized in phagocytic cells . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| The active oxidase consists of two membrane proteins , gp91phox and p22phox , and four cytosolic proteins , p40phox , p47phox , p67phox , and Rac . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| These changes were associated with increased mRNA expression of endothelin ( ET ) 1 and ET A receptor ( P < 0 . 05 ) , cyclooxygenase ( COX ) 2 / TXA2 synthase ( P < 0 . 05 ) , NADPH oxidase ( p 40 phox , p 67 phox , P < 0 . 05 ) and iNOS and nNOS ( P < 0 . 05 ) . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| This multicomponent enzyme system is composed of cytosolic proteins ( p47phox , p67phox , p40phox , and rac1 / 2 ) and membrane proteins ( p22phox and gp91phox , which form cytochrome b 558 ) which assemble at membrane sites upon cell activation . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| However , this production of O 2 is dependent on translocation of the oxidase subunits , including gp91phox , p22phox , p47phox , p67phox , p40phox , and Rac 2 from the cytosol or specific granules to the plasma membrane . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Active forms of NAD ( P ) H oxidases are associated with plasma membrane and consist of at least six components , namely : NOX , p22phox peptides and p47phox , p67phox , p40phox and Rac . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| Production of NOX 4 , p22phox , p47phox , p67phox , and p40phox was confirmed by Western blot . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| DOCA SHR was characterized by increased blood pressure ( BP ) ; increased kidney weight ; decreased renal function ; increased proteinuria ; abnormal histological findings ; increased monocyte / macrophage infiltration ; increased urinary 8 isoprostran levels ; increased gene expression of collagen 1 , collagen 3 , transforming growth factor beta , and reduced nicotinamide adenine dinucleotide phosphate oxidase subunits ( p40phox , p47phox , and p67phox ) ; and decreased gene expression of endothelial NO synthase ( eNOS ) in the renal cortex as compared with Wistar Kyoto rats . ^^^ Interestingly , long term fasudil treatment significantly decreased monocyte / macrophage infiltration and urinary 8 isoprostran excretion , in association with decreased mRNA levels of transforming growth factor beta , collagen 1 , collagen 3 , and NADPH oxidase subunits ( p40phox , p47phox , and p67phox ) , and increased mRNA levels of eNOS in the renal cortex . ^^^ |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15080 and P19878 |
Pubmed |
SVM Score :0.0 |
| NA |
|