| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| The human NADPH oxidase is a very complex enzyme , in two ways : 1 . it exists of at least 6 components : cytochrome b 558 ( a heterodimer comprised of gp 91 phox and p 22 phox ) , p 47 phox , p 67 phox , p 40 phox , rac and Rap1A , and 2 . there are multiple signal transduction pathways leading to activation of the NADPH oxidase . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| Following incubation of monocytes with IL 15 for 18 h , there was no significant increase in mRNA transcripts for components of the NADPH oxidase complex , p 40 phox , p 47 phox , and gp 91 phox , suggesting a posttranscriptional modulation of enhanced superoxide production . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| It is composed of several subunits , two of which , gp91phox and p22phox , form the membrane bound cytochrome b 558 , while its three cytosolic components , p47phox , p67phox and p40phox , have to translocate to the membrane upon activation . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| Taken together , these findings indicate that 1 ) a low expression of some NADPH oxidase components may represent the molecular basis of the low production of O 2 in B lymphocytes ; 2 ) the cytosolic components of NADPH oxidase can not bind to p22phox on the membranes in the absence of gp91phox ; 3 ) p47phox can translocate to the membranes independently of p67phox and p40phox ; and 4 ) gp91phox may have a role in mediating and / or stabilizing the binding of p67phox and p40phox to the membranes of activated cells . . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| It consists of membranous cytochrome b 558 composed of gp 91 phox and p 22 phox , and four cytosolic components , p 47 phox , p 67 phox , rac p 21 and p 40 phox , which translocate to the membrane upon activation . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| Upon stimulation , a functional NADPH oxidase is assembled when the cytosolic proteins , Rac , p67phox , p47phox , and possibly p40phox , associate with the gp91phox and p22phox transmembrane proteins . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| It consists of membranous cytochrom b 558 composed of gp 91 phox and p 22 phox , and four cytosolic components , p 47 phox , p 67 phox , rac p 21 and p 40 phox , which translocate to the membrane upon activation . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Superoxide generating NADPH oxidase consists of the membrane bound cytochrome b 558 ( gp91phox and p22Phox ) and the cytosolic components ( p67phox , p47phox , p40phox and rac ) . ^^^ RESULTS : Northern blot and Western blot analyses revealed that mRNAs and proteins for gp91phox , p67phox and p47phox were expressed after eosinophilic myelocyte stages , whereas mRNAs and proteins for p40phox and rac 2 were expressed from the promyelocyte stage . ^^^ Consistent with the results of Western blotting , immunocytochemical staining of butyrate induced HL 60 clone 15 cells indicated that gp91phox , p22phox , p67phox and p47phox were detected after eosinophilic myelocyte stages ( eosinophilic myelocytes , eosinophilic metamyelocytes , eosinophilic band cells and eosinophilic segmented cells ) , whereas p40phox and rac 2 were expressed from the promyelocyte stage . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| Treatment with atorvastatin reduced vascular mRNA expression of p22phox and nox 1 and increased aortic catalase expression . mRNA expression of superoxide dismutases , glutathione peroxidase , and NAD ( P ) H oxidase subunits gp91phox , p40phox , p47phox , and p67phox remained unchanged . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| We investigated whether VSMCs from human resistance arteries ( HVSMCs ) possess a functionally active , angiotensin 2 ( Ang 2 ) regulated NAD ( P ) H oxidase that contains neutrophil oxidase subunits , including p22phox , gp91phox , p40phox , p47phox , and p67phox . mRNA expression of gp91phox homologues , nox 1 and nox 4 , was also assessed in HVSMCs , human aortic smooth muscle cells , and rat VSMCs . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| This deficiency may have resulted from a gene mutation and / or abnormality of the NADH oxidase components , particularly cytochrome b 558 ( gp91phox and p22phox ) in membrane and cytosol factors p47phox , p67phox , p40phox and others in neutrophil . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase complex is composed of membrane proteins , p22PHOX and gp91PHOX , and cytosolic proteins , p40PHOX , p47PHOX and p67PHOX . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| Immunological assays have demonstrated the presence of p67phox and p47phox in lens tissue , while PCR and RT PCR reactions amplified DNA products corresponding to the p67phox , p40phox , p22phox , gp91phox , and Rac 1 components of the NADPH oxidase complex from human and mouse lens cDNA libraries . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| The active oxidase consists of two membrane proteins , gp91phox and p22phox , and four cytosolic proteins , p40phox , p47phox , p67phox , and Rac . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| This multicomponent enzyme system is composed of cytosolic proteins ( p47phox , p67phox , p40phox , and rac1 / 2 ) and membrane proteins ( p22phox and gp91phox , which form cytochrome b 558 ) which assemble at membrane sites upon cell activation . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| However , this production of O 2 is dependent on translocation of the oxidase subunits , including gp91phox , p22phox , p47phox , p67phox , p40phox , and Rac 2 from the cytosol or specific granules to the plasma membrane . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| RESULTS : DS rats were characterized by increased blood pressure , decreased renal function , abnormal histological findings , and increased gene expression of collagen 1 and 3 , transforming growth factor beta ( TGF beta ) , and NADPH oxidase subunits ( p40phox , p47phox , and gp91phox ) in the renal cortex compared with DR rats . ^^^ Only combined treatment decreased mRNA levels of p40phox , p47phox , and gp91phox . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| In addition , RT PCR revealed that SH SY5Y cells expressed mRNA of gp91phox , p22phox and cytosolic p47phox , p67phox and p40phox , the components of the plasma membrane NADPH oxidase . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase consists of a membrane heterodimeric protein , composed of gp91phox and p22phox , and cytosolic proteins , p40phox , p47phox and p67phox . ^^^ The sequences of these cDNAs showed that the pufferfish gp91phox , p22phox , p40phox , p47phox and p67phox clones contained open reading frames encoding 565 , 186 , 348 , 423 and 495 amino acids , respectively . ^^^ Comparison of the deduced amino acid sequences showed that the pufferfish gp91phox , p22phox , p40phox , p47phox and p67phox sequences shared 68 . 0 , 61 . 8 , 53 . 8 , 54 . 7 and 41 . 9 % identity with those of human components , respectively . gp91phox has three potential N linked glycosylation sites . gp91phox and p22phox have six and three hydrophobic regions , respectively , that are predicted to be transmembrane regions . p47phox and p67phox have two potential Src homology 3 domains and p40phox has one . ^^^ The level of gp91phox , p47phox and p67phox expression were high only in the blood and kidney , while p22phox and p40phox were constitutively expressed in a wide range of tissues . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| Finally , increases in the RVLM mRNA and protein expression of Ang 2 type 1 ( AT 1 ) receptor and subunits of NAD ( P ) H oxidase ( p40phox , p47phox , and gp91phox ) were demonstrated in CHF rabbits . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NADPH oxidase is multi component enzyme , which comprises the cytosolic proteins p40Phox , p47Phox , and p67Phox and the two membrane proteins , gp91Phox and p22Phox , and which is well characterized in phagocytic cells . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| The primary association of p40phox appears to be with p67phox , and it is present in reduced amounts in patients with CGD lacking p67phox . . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| In this paper we show that ( a ) in neutrophils from a patient with a form of chronic granulomatous disease ( CGD ) in which p67phox is absent , p47phox and Rac 2 , but not p40phox and Rac 1 were translocated from the cytosol to the membrane on stimulation with formylmethionyl leucylphenylalanine ( fMLP ) or phorbol 12 myristate 13 acetate ( PMA ) ; ( b ) in neutrophils from a patient with a form of CGD in which p47phox is absent , p67phox , p40phox and Rac 1 failed to associate with the membrane on stimulation with fMLP or PMA , whereas Rac 2 was translocated as in normal neutrophils . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| Although it has been found that this p40phox protein is defective in patients with chronic granulomatous disease ( CGD ) who lack p67phox , evidence to functionally relate it to the NADPH oxidase system has hitherto been lacking . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| Reconstitution of oxidase activity was performed in vitro by using a heterologous cell free assay consisting of membrane suspended or solubilized and purified cytochrome b 558 that was associated with cytosol or with the isolated cytosolic activating factors ( p 67 phox , p 47 phox , p 40 phox ) from healthy or CGD patients . ^^^ In p 67 phox deficient CGD patients , two cytosolic factors are deficient or missing : p 67 phox and p 40 phox . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| Examination of 45 non synonymous polymorphisms in ten genes : p47phox ( NCF 1 ) , p67phox ( NCF 2 ) , p40phox ( NCF 4 ) , p22phox ( CYBA ) , gp91phox ( CYBB ) , DUOX 1 , DUOX 2 , TLR 2 , TLR 9 and alpha 1 antitrypsin ( AAT ) in a cohort of 95 lung disease individuals and 95 control individuals did not show an association of these polymorphisms with increased susceptibility to lung disease . . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| In this report we describe a novel cytosolic component , p40phox , which consistently behaves with p67phox through immunoprecipitation and column works , and is missing in patients with CGD who lack p67phox . ^^^ |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04839 and Q15080 |
Pubmed |
SVM Score :0.0 |
| NA |
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