| With this approach , the nuclear protein SET was shown to interact with the N terminal regions of p 35 ( nck5a ) and p 39 ( nck5ai ) . ^^^ Our detailed characterization showed that the SET protein formed a complex with Cdk5 / p35 ( nck5a ) through its binding to p 35 ( nck5a ) . ^^^ The p 35 ( nck5a ) interacting region was mapped to a predicted alpha helix in SET . ^^^ When cotransfected into COS 7 cells , SET and p 35 ( nck5a ) displayed overlapping intracellular distribution in the nucleus . ^^^ The nuclear co localization was corroborated by immunostaining data of endogenous SET and Cdk5 / p35 ( nck5a ) from cultured cortical neurons . ^^^ |