| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.64524906 |
| In addition , p35nck5a was colocalized with cdk 5 immunoreactivity in LB . 0.64524906^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.69768296 |
| The results of this study indicate that p35Nck5a is associated with Cdk 5 in monocytic differentiation of hematopoietic cells . 0.69768296^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| By extensive genetic analysis of the genes encoding CDK 5 ( CDK 5 ) , p 35 ( CDK5R1 ) and p 39 ( CDK5R2 ) , we excluded causal mutations in 70 familial early onset AD patients . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| The present study describes a significant amino acid sequence homology between neuronal Cdk 5 activator ( nck5a ) and an open reading frame of an unknown gene on the yeast S . cerevisiae chromosome 3 . ^^^ GST p25Y was found to block the in vitro activation of Cdk 5 by nck5a and to affinity precipitate Cdk 5 from bovine brain extract . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| In this study , we probe the relationship between the two different forms of Nck5a and their interaction with and activation of Cdk 5 in bovine brain extract . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| The kinase was found to inactivate Cdc2 / cyclin B , Cdk2 / cyclin A , and neuronal Cdc 2 like kinase ( Nclk ) , a heterodimer of Cdk 5 and neuronal Cdk 5 activator ( Nck5a ) , under phosphorylation conditions . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| Here we report that neuronal Cdc 2 like kinase ( Nclk ) , composed of Cdk 5 and a neuronal Cdk 5 activator ( p 25 ( nck5a ) ) , can bind and phosphorylate RB . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| Neuronal Cdk 5 activator ( Nck5a ) differs from other cyclin dependent kinase ( Cdk ) activators in that its amino acid sequence is only marginally similar to the cyclin consensus sequence . ^^^ In the present study , a number of truncation mutants and substitution mutants of the Nck5a were produced and tested for the Cdk 5 activation and Cdk 5 binding activity . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| In addition to the activation by Nck5a , Cdk 5 is regulated by other mechanisms including additional activator proteins and inhibition by phosphorylation of specific amino acid residues . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| The changes in Cdk 5 and Nck5a expression appear to correlate with the terminal differentiation of neurons of the mouse embryonic brain . ^^^ Transfection of cultured cortical neurons with dominant negative cdk 5 mutants or Nck5a antisense DNA may reduce neurite growth , suggesting that Nclk plays an active role in neuron differentiation . ^^^ The mechanism of activation of Cdk 5 by Nck5a differs from that of cyclin activation of Cdks in that full Cdk 5 kinase activity can be achieved in the absence of phosphorylation of Cdk 5 . ^^^ An isoform of Nck5a , a 39 kDa protein has been cloned and shown to share extensive amino acid identity and the mechanism of Cdk 5 activation with Nck5a . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| The activation of cyclin dependent kinase 5 ( Cdk 5 ) depends on the binding of its neuronal specific activator Nck5a . ^^^ In this work we show that a 29 residue peptide , denoted as the alphaN peptide , encompassing amino acid residues Gln 145 to Asp 173 of Nck5a is capable of binding Cdk 5 to result in kinase inhibition . ^^^ Direct competition experiments have shown that this inhibitory peptide does not compete with Nck5a or cyclin A for Cdk 5 or Cdk 2 , respectively . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| Tau protein kinase 2 ( TPKII ) is a heterodimer comprising a catalytic cyclin dependent kinase subunit ( Cdk 5 ) and a regulatory protein called neuronal Cdk 5 activator ( Nck5a ) . ^^^ Based on this structural inference , a 3 dimensional model of the Cdk 5 Nck5a * ATP complex was derived from the 10 ray structure of Cdk 2 cyclinA ATP complex . ^^^ The computed structure for TPKII is fully compatible with experimental data derived from studies of the Cdk 5 Nck5a system , and also predicts which amino acid residues might be involved in formation of the Cdk 5 Nck5a * interface and ATP binding pocket in TPKII . ^^^ The computational structure also shows the interactive region of Nck5a * and the T loop of Cdk 5 , a critical region in TPKII which functions as a gate control lever of the catalytic cleft . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| To date , CDK 5 purified from mammalian brains has always been associated with a truncated form of the 35 kDa major brain specific activator ( p 35 , also known as nck5a ) of CDK 5 , known as p 25 . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| We explored these issues by chromatographic and immunochemical analyses of Cdk 5 and p 25 ( nck5a ) ( a neuronal Cdk 5 activator ) and their associated proteins from bovine brain . ^^^ The majority of p 25 ( nck5a ) from Mono S fractions 1 , 2 , and 3 eluted from the gel filtration column at approximately 60 , 200 , and 400 kDa , respectively , and Cdk 5 was abundant in fractions > 400 kDa . ^^^ We characterized these macromolecular structures by immunoprecipitating p 25 ( nck5a ) , followed by a second immunoprecipitation of remaining unbound proteins using a Cdk 5 antibody . ^^^ The p 25 ( nck5a ) immunoprecipitates showed association with Cdk 5 . ^^^ The Cdk 5 immunoprecipitates , however , revealed abundant retained Cdk 5 but no remaining p 25 ( nck5a ) , indicating that Cdk 5 in macromolecular structures is mostly unassociated with p 25 ( nck5a ) . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| Cyclin dependent protein kinase 5 ( Cdk 5 ) depends on the association with neuronal Cdk 5 activator ( Nck5a ) for kinase activity . ^^^ This region of Nck5a contains an amphipathic alpha helix whose hydrophobic face is involved in Cdk 5 activation ( Chin , K . ^^^ First , the Nck5a ( 145 170 ) peptide can bind Cdk 5 and Nck5a binding proteins simultaneously . ^^^ Second , the association of Nck5a ( 145 170 ) to C 48 can be markedly reduced by high ionic strength whereas the interaction between Nck5a and Cdk 5 is not affected . ^^^ Third , substitution of Glu ( 157 ) by glutamine in Nck5a ( 145 170 ) abolishes the peptide ' s ability to bind to the three Nck5a binding proteins without diminishing its Cdk 5 binding activity . . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| To test the hypothesis that a complex of cyclin dependent kinase 5 ( Cdk 5 ) and p 35 ( nck5a ) plays an important role in sprouting in the kindling rat hippocampus , we studied the changes in kinase activity , expression level and subcellular localization during kindling progression . ^^^ These findings suggest that Cdk5 / p35 ( nck5a ) plays an important role in synaptic reorganization , and the translocation of Cdk 5 to soma from axons is a crucial regulatory mechanism of kinase activity . . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| Structure and regulation of the CDK 5 p25 ( nck5a ) complex . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| The neuronal Cdk 5 kinase is composed of the catalytic subunit Cdk 5 and the activator protein p 35 ( nck5a ) or its isoform , p 39 ( nck5ai ) . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| The active kinase consists of a catalytic subunit , Cdk 5 , and an essential regulatory subunit , neuronal Cdk 5 activator ( p 35 ( nck5a ) or p 25 ( nck5a ) ) , which is expressed primarily in neurons of central nervous tissue . ^^^ Here we show that one of these proteins , called C 42 , specifically inhibits the activation of Cdk 5 by Nck5a . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| The isoform , designated the neuronal Cdk 5 activator isoform ( p39nck5ai ) , showed a high degree of sequence similarity to p35nck5a with 57 % amino acid identity . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| These developmental changes in p35nck5a expression correlated with the changes in Cdk 5 associated kinase activity during brain development . ^^^ These data suggest that p35nck5a is the specific activator for Cdk 5 in the brain . ^^^ Our findings suggest that p35nck5a is neuron specific , and a specific activator for Cdk 5 , and the subcellular localization of the two is strictly regulated depending on brain development . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| Distinct cellular compartment of cyclin dependent kinase 5 ( Cdk 5 ) and neuron specific Cdk 5 activator protein ( p35nck5a ) in the developing rat cerebellum . ^^^ We have elucidated the spatial and temporal localization of Cdk 5 and p35nck5a in the developing rat postnatal cerebellum . ^^^ The Cdk 5 kinase activity was correlated with the expression of p35nck5a rather than that of Cdk 5 . ^^^ These results indicate that p35nck5a is a physiological activator of Cdk 5 in immature neurons and further suggest that Cdk 5 has another function in mature neurons . . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| Developmental alteration of the expression and kinase activity of cyclin dependent kinase 5 ( Cdk 5 ) / p35nck5a in the rat retina . ^^^ This kinase is a heterodimer of Cdk 5 and p35nck5a and influences neuronal maturation or sprouting in the normal brain . ^^^ The expression of Cdk 5 and p35nck5a increased between 1 week and 3 weeks after birth . ^^^ The developmental change of Cdk5 / p35nck5a kinase activity coincided with those of the expression of p35nck5a and Cdk 5 . ^^^ The expression levels of Cdk 5 and p35nck5a in retina of rats raised from fetus to 3 weeks after birth in darkness were 36 % and 40 % respectively , of the baseline for control rats . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| Both cdk 5 and p35nck5a are present and associated in proliferative stages , although cdk 5 p35 kinase activity is barely detectable . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| Cdk 5 exists in brain extracts in multiple forms , one of which is a macromolecular protein complex comprising Cdk 5 , neuron specific Cdk 5 activator p35nck5a and other protein components ( Lee , K . ^^^ The p35nck5a binding site on NF M was mapped to a carboxyl terminal region of the rod domain , in close proximity to the putative Cdk 5 phosphorylation sites in NF M . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| The active kinase consists of a catalytic subunit , Cdk 5 , and a 25 kDa activator protein ( p25nck5a ) derived from a 35 kDa neuronal specific protein ( p35nck5a ) . ^^^ The bacterially expressed glutathione S transferase ( GST ) fusion forms of these three proteins were able to co precipitate p35nck5a complexed with Cdk 5 from insect cell lysate . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| METHODS : Changes kinase activity , expression levels and subcellular localizations of Cdk 5 and p35nck5a in the rat hippocampus were studied during kindling progression by Western blot analysis , immunohistochemistry , immunoprecipitation and kinase assay . ^^^ Cdk 5 was translocated from axon to soma when kinase activity was high . p35nck5a was always localized in the soma throughout kindling progression . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| The neuronal cyclin dependent kinase 5 activator p35Nck5a and Cdk 5 activity in monocytic cells . ^^^ Cyclin dependent kinase 5 ( Cdk 5 ) and its activator p35Nck5a are an odd but interesting couple . ^^^ Recent work has established that in spite of their names , Cdk 5 is clearly not cyclin dependent , while p35Nck5a is not exclusively neuronal . ^^^ Indeed , p35Nck5a and / or Cdk 5 activity has been demonstrated to be present in human and rat promyelocytic cell lines , and in normal human monocytes . ^^^ Further , monocytes from mice in which p35Nck5a gene was deleted do not express non specific esterase , an enzyme present in normal monocytes , while transfection of Cdk 5 together with p35Nck5a into myeloblastic cells leads to the expression of this marker of the monocytic phenotype . ^^^ |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15078 and Q00535 |
Pubmed |
SVM Score :0.0 |
| NA |
|